Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913422,4320TG22.3% 754.1 / 55.0 344intergenic (+73/‑86)proY/malZproline‑specific permease/maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (76/186);  new base G (75/0);  total (157/187)
Fisher's exact test for biased strand distribution p-value = 9.15e-33
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 357 total aligned reads displayed.

TCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCAT  >  NC_000913/422283‑422582
                                                                                                                                                     |                                                                                                                                                      
gcgcgTGGATTGTTGGGCTGTTGATTGGCGGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTTAAAACCAGTAATCCATGCGTTCTGTAAGCCGGTTCAGGAGGAAATGCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtcctc                                                                                                                                                       <  7:769415/150‑1 (MQ=255)
  gcgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGgccgccg                                                                                                                                                     >  6:974152/1‑150 (MQ=255)
        aTTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGccccc                                                                                                                                                >  7:1190836/1‑150 (MQ=255)
           gttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGgcctggggcccccaa                                                                                                                                             >  7:289809/1‑139 (MQ=255)
              gtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCGTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATc                                                                                                                                         <  5:683556/151‑1 (MQ=255)
              gtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAatat                                                                                                                                          <  7:572001/150‑1 (MQ=255)
                gCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATcc                                                                                                                                        <  3:357370/150‑1 (MQ=255)
                      gATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgat                                                                                                                                 <  1:908477/151‑1 (MQ=255)
                       aTTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAAAAACCATCAcatg                                                                                                                                >  3:860265/1‑151 (MQ=255)
                       aTTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCTTCAgatt                                                                                                                                >  4:870226/1‑150 (MQ=255)
                          ggCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgatgat                                                                                                                              <  6:1420026/150‑1 (MQ=255)
                          ggCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgatgat                                                                                                                              <  2:1086724/150‑1 (MQ=255)
                          ggCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgatgat                                                                                                                              <  3:542490/150‑1 (MQ=255)
                           gCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGAtgatga                                                                                                                            <  6:1253853/151‑1 (MQ=255)
                            cTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCTTCAGAggatgat                                                                                                                           >  1:308832/1‑150 (MQ=255)
                              ggATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAg                                                                                                                           <  2:596575/149‑1 (MQ=255)
                              ggATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGt                                                                                                                          <  4:370826/150‑1 (MQ=255)
                                   tttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAAGATCCTTCAGCTGAGGACTGCTccg                                                                                                                    >  2:258038/1‑150 (MQ=255)
                                    ttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCt                                                                                                                    <  7:351153/150‑1 (MQ=255)
                                     tAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTg                                                                                                                   <  8:1281859/150‑1 (MQ=255)
                                       aaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCTTTCTGTAAGCCGGTTCAGGAGTAAATCCTGATCCGGCTATTCCTAAAGTTATCCGCCCACGTCCTCCTACACCAAATATCCTTCAGATGATGAGTGATCCTGCAt                                                                                                                <  6:514270/151‑1 (MQ=255)
                                          gccgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAg                                                                                                             <  5:1367906/151‑1 (MQ=255)
                                            cgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCCTGCTCGCCCAAATATCCTTCAGATGATGGGTGGTCCGGCAGTAGGc                                                                                                           >  8:836241/1‑151 (MQ=255)
                                             gccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCt                                                                                                          <  1:851509/151‑1 (MQ=255)
                                             gccACGATCGGCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGGGATCCTGCATTAGGCt                                                                                                          <  5:806945/151‑1 (MQ=255)
                                              ccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGGGGCGGCCGCGGCCCCCACATCGCCGTCCGATGATGCGTGATGCTggcgtaggcgt                                                                                                         >  8:442668/1‑141 (MQ=255)
                                               cACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTAt                                                                                                        <  4:1251594/151‑1 (MQ=255)
                                                 cGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGGCGCCCCCCAATATCCTTCAGATGATGAGTGATCCTGCGGTAGGCTATgg                                                                                                      >  8:1370997/1‑151 (MQ=255)
                                                  gATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGGCGCCGCGCCCAACGAGCCTTGAGAGGATGAGTGCTCCTGCATGAGGCTATGGc                                                                                                     >  5:429406/1‑151 (MQ=255)
                                                  gATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGc                                                                                                     <  5:877091/151‑1 (MQ=255)
                                                  gATCGTCAGCTGGCAGAAAACCAGTAATCCTTCCCTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCAGGCTATTCCGAAAGTTATCAGCCCCGCAGGACATCCCACAAATATCCGAAAGATGATGAGAGTTCCAGccaaacgaaatcg                                                                                                      >  4:565241/1‑138 (MQ=255)
                                                   aTCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGGCGTCCGCGCCCCACGAACGAGCAGAGGATGAGGGATGCTGCAGTAGGGTATggg                                                                                                     >  1:677555/1‑149 (MQ=255)
                                                   aTCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCCGCGGCCGCCCCCCAATAACCTTCAAAAGCTGAGGGGTCCTGCATTAggaaatgccg                                                                                                    >  1:63518/1‑143 (MQ=255)
                                                     cGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAg                                                                                                  <  6:1216430/151‑1 (MQ=255)
                                                        cAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGt                                                                                                <  6:351046/150‑1 (MQ=255)
                                                             ggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGGCTGCGCCCGCAAATATGCGTCAGATGATGAGTGATGCTGGATTAGGGTATGGGAAGGTGAtg                                                                                            >  7:21722/1‑148 (MQ=255)
                                                             ggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCGGCCGCCCCCAAATATCATTCAGATGATGAGGGATGCTGCATTAGGCGATGGGAAGGGGAtgcg                                                                                          >  6:104700/1‑148 (MQ=255)
                                                              gctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGa                                                                                         <  2:7274/151‑1 (MQ=255)
                                                                tgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGGCCCCCAATATCCTTCACTTGATGCGTGGTCCTGGCTTAGTCTCTGGCATGTGGCTCAGAtt                                                                                       >  5:994022/1‑151 (MQ=255)
                                                                 gAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCAGTAGGCTATGGGAGGGGGCTCAGAttt                                                                                      >  3:622599/1‑151 (MQ=255)
                                                                  aaaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCGGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGAGCAGAtttt                                                                                     >  4:18907/1‑151 (MQ=255)
                                                                   aaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATGCGGGGGCGGCCAGCTCCCCCAAAAATAGGAGAGCTGTGGAGGGATGCAGCATTAGGCTATTGGAAGGTGTTCAGAttttt                                                                                    >  8:1402288/1‑150 (MQ=255)
                                                                   aaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtc                                                                                    <  6:321081/151‑1 (MQ=255)
                                                                     aCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCGCCTCCCCCCCATACCCATCAGATCATGAGTGATCCTGCATTAGGGTATGGCAAGGTGATGAGATTTtcat                                                                                  >  2:539943/1‑151 (MQ=255)
                                                                     aCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcat                                                                                  <  5:1322683/151‑1 (MQ=255)
                                                                      ccAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTGCGCCCCCCAATATCCTTCAGATGATGAGTGATGCTGCATTAGGGTATGGGAAGGTGATCAGATTTtcatc                                                                                 >  8:766098/1‑151 (MQ=255)
                                                                           aaTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgg                                                                            <  4:1074871/151‑1 (MQ=255)
                                                                                ttGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCAGGCTATTCCGAAAGTTATCCGCCCGCGTCGGCCGGCCCCAACGATACGACAGAGTAGGCGGGATCCGGCAGTAGGATATTGCAAGGGGGGCAGATGTTCATGACACGGGact                                                                       >  4:269233/1‑149 (MQ=255)
                                                                                 tGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCGAATATCCTTCAGCTGATGAGTGCTCCGGGGTTGGGGTATGGGCAGGGGAGGAGAttttagtgagggggggtgt                                                                      >  4:774311/1‑136 (MQ=255)
                                                                                  gCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAAAATCCTTCAGATGATGAGTGATGCTGCATTAGGGGATGGCAAGGTGGTCAGATTTTCATGACAGGGGaagtt                                                                     >  8:634081/1‑148 (MQ=255)
                                                                                   cGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTat                                                                    <  1:408617/151‑1 (MQ=255)
                                                                                    gTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGCTGCTGGGTTAGGCTTTGGCACCGGGATCAGATTTTCATCACAGGGGATTTatt                                                                   >  2:1190433/1‑150 (MQ=255)
                                                                                    gTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatg                                                                   <  6:717624/151‑1 (MQ=255)
                                                                                        tGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGGCGCCTCCCCCAAAGAAACAACACATGATGAGTGAGACTGCAGTAGGCTAGGGCAAGGTGCTCAGATTTTATTCTCAGGGGAATTATTATGt                                                               >  2:892325/1‑151 (MQ=255)
                                                                                           aaGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATACTTAAGATGATGAGTGATCGTGAATTAGGCTATGGCAAGGTGATGAGATTTTAATGAAAGGGGAATTATGATGttt                                                             >  3:305982/1‑149 (MQ=255)
                                                                                            aGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGGCCACCTGCCCCAAATATCCTTCAGATGATGAGTGATGCTGCAGTATGGTATGGGAAGGTGATAAGATTTTGATCAGAGGGGAATTATGATGtttat                                                           >  1:761397/1‑148 (MQ=255)
                                                                                             cccGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAAt                                                          <  2:968592/150‑1 (MQ=255)
                                                                                              ccGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATg                                                         <  5:327396/151‑1 (MQ=255)
                                                                                              ccGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATg                                                         <  6:210511/151‑1 (MQ=255)
                                                                                              ccGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATg                                                         <  8:1338697/151‑1 (MQ=255)
                                                                                               cGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGc                                                        <  2:121295/151‑1 (MQ=255)
                                                                                               cGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATAGACTTCAGATGATGAGTGATGCTGCATTAGCCTAAGGAAAGGTGATAAGATTTTCATCACAGGCGAATTATGATGTTAATTGc                                                        >  6:587726/1‑151 (MQ=255)
                                                                                                  ttCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTGCTCCGCCCCCCCAAATACTTCAGATGATGAGTGATCGTGGATTAGGGTATGGGAAGGTGATGAGATTTTCATCACCGGGGAGTTTTTATGTTTAATGCATg                                                     >  7:388022/1‑151 (MQ=255)
                                                                                                     aggaggAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGGCCGGCCGCCGCCCCCCCATATCCTTCAGATGATGATTGATGCTGCATTAGGCTATGGCAAGGTGATCAGATTTTGATCACAGGGGAATTATGATGTTAAATGCATTGc                                                   >  2:1040581/1‑150 (MQ=255)
                                                                                                         ggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCCGCGGCCCCCAAATATACTTCAGATGATGACTTAAACAGCCTGAGGCTATGGCAATGTGATCAGCTTTTCATAAAAGGCGAATTATGATGTTAAATTCATgtctactg                                              >  5:1421635/1‑144 (MQ=255)
                                                                                                         ggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCATTCAGATGATGAGTGATCCTGCATTAGGGTATGGGAAGGTGATAAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTg                                              >  1:521484/1‑151 (MQ=255)
                                                                                                         ggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAAAATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGCTCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGccccgg                                              >  5:10024/1‑149 (MQ=255)
                                                                                                           aaaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGGGATCAGATTTTCATAACAGGGGAATTATGATGTTAAATGCATGGCGCCGGcc                                            >  1:293255/1‑151 (MQ=255)
                                                                                                            aaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCCAATAACCTTCAGATGATGAGTGAGCCTGCATTAGGCGATGGGAAGGTGAGCAGCTTTTAATCACAGGGGAATTATGATTTTAAATGAATGGCACCTGCCg                                           >  8:151968/1‑151 (MQ=255)
                                                                                                               ccTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCGCCGCCCCCAAATATCCTTCAGATGAGGAGTGCTGCGGGAGGTGTCGATGGGACGGTGATCCGAGTTTCATGACAGGGGAATGATGATGTTTAGTGCATGGCGCCGGCCGGTg                                        >  3:950183/1‑151 (MQ=255)
                                                                                                                      cGGCTATTCCGAAAGTTATCCGCCCCCGTCCTGCGCCCCCAAATATACTTCAGATGATGAGTGATCCTGCATTAGGGTATGGCAAGGTGATCAGATTTTGATAACAGGGGAATTTTGATGTTATATTCATGGCACCTGCCGGTGGCCCCAt                                 >  4:652000/1‑151 (MQ=255)
                                                                                                                        gCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCAttt                               <  2:1416803/151‑1 (MQ=255)
                                                                                                                        gCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAAAAACCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTATTCACAGGGGAATTATGATGTTAAATGCAAGGCACCTGCCGGTGACCCCAatt                               >  6:771359/1‑151 (MQ=255)
                                                                                                                         cTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAACTATCCTTCAGATGATGAGTGATCCTGCATTAGGCGATGGCAAGGTGATCAGATTTTCATCACAGCGGAAGTATTATGTTAAATTAATGTCACCTGCCGGTGCCCCCATATg                              >  8:242604/1‑151 (MQ=255)
                                                                                                                          tATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAATATCCTTAATAAGATGAGTGATCCTGCATTAGTCAATGGGAAGGTGATCAGATTTTGATAACATGGGAATTATGATGTTAAATAAATGGCACCTCACCGAGCCGCCAGTTGt                             >  1:1035827/1‑151 (MQ=255)
                                                                                                                           aTTCCGAAAGTTATCCGCCCCCTTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTg                              <  8:121772/149‑1 (MQ=255)
                                                                                                                           aTTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCGTGGCACCTGCCGGTGCCCCCATTTGt                             <  3:1262844/150‑1 (MQ=255)
                                                                                                                           aTTCCGAAAGTTATCCCCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTg                              <  6:1144327/149‑1 (MQ=255)
                                                                                                                              ccGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTATGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTaaa                         <  2:434176/151‑1 (MQ=255)
                                                                                                                              ccGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTaaa                         <  7:524289/151‑1 (MQ=255)
                                                                                                                               cGAAAGTTATCCGCCCGCGGGCGCCTCCCCCAAAAATCATTAAGATGAAGAGTGATACTGAAATAGTGTATGGCAAGGTGATCAGATTTTAATAACAGGTGAATTATAATTTTAAAATAATTGAACATGACGGTGCCCCCATATCTTaaa                         >  4:44915/1‑150 (MQ=255)
                                                                                                                               cGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAc                        <  2:131723/151‑1 (MQ=255)
                                                                                                                                    gTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagc                   <  7:979126/151‑1 (MQ=255)
                                                                                                                                    gTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagc                   <  8:497580/151‑1 (MQ=255)
                                                                                                                                    gTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagc                   <  6:913409/151‑1 (MQ=255)
                                                                                                                                      tATCCGCCCCCCGCCTCCTCCCCCAAATATACTTCAGATGATGAGTGATCCTGCATTAGTCTATGGCAAGGTGATCAGATTTTAATAACAGGGGAATTATGATGTTAAATGAATGGCACCAGCCGGTGCCCCCAATTGTTAAAaaaagca                  >  3:109227/1‑150 (MQ=255)
                                                                                                                                         ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaaag               <  5:526356/150‑1 (MQ=255)
                                                                                                                                         ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaaag               <  2:194542/150‑1 (MQ=255)
                                                                                                                                          cGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGAt             <  6:1324033/151‑1 (MQ=255)
                                                                                                                                            cccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATc            >  5:562104/1‑150 (MQ=255)
                                                                                                                                            cccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCa           <  8:87589/151‑1 (MQ=255)
                                                                                                                                             ccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCa           >  7:896848/1‑150 (MQ=255)
                                                                                                                                               ccGTCGTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt        <  6:1172346/151‑1 (MQ=255)
                                                                                                                                               ccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt        >  5:1144397/1‑151 (MQ=255)
                                                                                                                                                 gTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGc      >  2:803268/1‑151 (MQ=255)
                                                                                                                                                 gTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGc      >  3:452427/1‑151 (MQ=255)
                                                                                                                                                 gTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGc      >  2:1231862/1‑151 (MQ=255)
                                                                                                                                                 gTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGc      >  6:826176/1‑151 (MQ=255)
                                                                                                                                                    ctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTc    >  8:601046/1‑150 (MQ=255)
                                                                                                                                                    ctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACATGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTtctc    >  1:1212242/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
TCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCAT  >  NC_000913/422283‑422582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑