Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913422,4350TG21.8% 772.5 / 51.1 344intergenic (+76/‑83)proY/malZproline‑specific permease/maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (79/186);  new base G (74/0);  total (157/187)
Fisher's exact test for biased strand distribution p-value = 7.20e-32
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 358 total aligned reads displayed.

GTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATT  >  NC_000913/422287‑422583
                                                                                                                                                    |                                                                                                                                                    
gTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGacctccgcc                                                                                                                                                    >  6:908177/1‑151 (MQ=255)
  ggATGGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGcccc                                                                                                                                                  >  3:1010622/1‑151 (MQ=255)
     ttgttgTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCa                                                                                                                                                >  4:21361/1‑150 (MQ=255)
     ttgttgTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGAACTCCTACcaca                                                                                                                                                >  4:975966/1‑150 (MQ=255)
       gttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCGCCAAat                                                                                                                                             >  5:410912/1‑151 (MQ=255)
       gttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAat                                                                                                                                             <  8:1411391/151‑1 (MQ=255)
               gTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAATATCCTTcc                                                                                                                                     >  2:1359012/1‑150 (MQ=255)
               gTTGATTGGCGGGATGATTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCtt                                                                                                                                       <  8:288614/149‑1 (MQ=255)
                ttGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCCAATATCCTTc                                                                                                                                      >  5:1257394/1‑149 (MQ=255)
                  gATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgat                                                                                                                                  <  8:612817/151‑1 (MQ=255)
                  gATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGGCGCGTCCCCCCAATATCCttg                                                                                                                                      >  5:512801/1‑146 (MQ=255)
                   aTTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCACCCCCAAATATCCTTCAgatg                                                                                                                                 >  1:308389/1‑151 (MQ=255)
                     tGGCTGGATGTGTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgatga                                                                                                                                <  5:1082930/150‑1 (MQ=255)
                      ggCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAACTATCCTTCAgatgatt                                                                                                                              >  6:1150826/1‑150 (MQ=255)
                           gATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGa                                                                                                                         <  2:1076597/151‑1 (MQ=255)
                             tttttAAACTCCGGCACGATCGTCATCTGTCTGAAAACCATTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATGCTGATCCGTCTATTCCGAAAGTTATCCGCCCACGTCCTCCTCCCCCAAATATCCTTGAGATGATTAGTGAt                                                                                                                        <  4:629625/148‑1 (MQ=255)
                              gTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATc                                                                                                                       <  6:467770/150‑1 (MQ=255)
                               tttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAAGATCCTTCAGCTGAGGACTGCTccg                                                                                                                     >  2:258038/1‑150 (MQ=255)
                                ttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCt                                                                                                                     <  7:351153/150‑1 (MQ=255)
                                ttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGACCGCCGCCCCCGAATAACCATCAGAGGAAGAGGGAtgcgg                                                                                                                    >  1:1479830/1‑147 (MQ=255)
                                  aaaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCAGc                                                                                                                   <  2:592600/150‑1 (MQ=255)
                                   aaaggcggcaagATGGTGAGGTGGCTGAAAAACAGTAATCCTTTGGTTCTGTAAGCCGGTTCATGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCa                                                                                                                  <  4:124183/139‑1 (MQ=255)
                                    aCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCAtt                                                                                                                <  3:348898/151‑1 (MQ=255)
                                     cgccgcCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCAAAAGTTATCCGCCCCCGGCCTCCTGCCCCAAATATCCTTCAGATGCTGAGTGATCCTGCCtt                                                                                                                >  7:630421/1‑150 (MQ=255)
                                      gccgccACGCGCGTCAGATGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGTAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAg                                                                                                              <  5:1361014/151‑1 (MQ=255)
                                      gccgccACGATCGTCAGCTGGCTGAAAAGCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAg                                                                                                              <  7:1013376/151‑1 (MQ=255)
                                          ccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGGGGCGGCCGCGGCCCCCACATCGCCGTCCGATGATGCGTGATGCTggcgtaggcgt                                                                                                          >  8:442668/1‑141 (MQ=255)
                                           cACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGGCCCCCAATATACTTCAGATCATTAGTGATGCTGCCTTAGGGTAt                                                                                                         >  2:755701/1‑151 (MQ=255)
                                            aCGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGGCCCCAAAGAACCTTCAGATCATGAGTGATCCTGCTTTAGGCTATg                                                                                                        >  6:1321755/1‑151 (MQ=255)
                                            aCGAGCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTGCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATg                                                                                                        <  5:238751/151‑1 (MQ=255)
                                             cGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCGCGGCCGCCGGCCGCGACGATCCGAGAGATGCTGAGTGATGCTGCATTAGGCGATgg                                                                                                       >  4:310579/1‑151 (MQ=255)
                                               aTCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGGCGTCCGCGCCCCACGAACGAGCAGAGGATGAGGGATGCTGCAGTAGGGTATggg                                                                                                      >  1:677555/1‑149 (MQ=255)
                                                 cGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAg                                                                                                   <  1:82944/151‑1 (MQ=255)
                                                 cGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAg                                                                                                   <  7:783364/151‑1 (MQ=255)
                                                 cGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATggga                                                                                                     >  7:917981/1‑147 (MQ=255)
                                                    cAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTg                                                                                                <  1:35817/151‑1 (MQ=255)
                                                      gctggctgAATACCAGTTATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATGCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGAt                                                                                              <  8:1357951/151‑1 (MQ=255)
                                                         ggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGGCTGCGCCCGCAAATATGCGTCAGATGATGAGTGATGCTGGATTAGGGTATGGGAAGGTGAtg                                                                                             >  7:21722/1‑148 (MQ=255)
                                                          gctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGACAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGa                                                                                          <  3:430269/151‑1 (MQ=255)
                                                           gtgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAt                                                                                         <  7:429513/150‑1 (MQ=255)
                                                           ctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAATATCCGTCAGATGATGAGTGATCCTGCATTAGGGTATGGCAAGGTGAGCGGAt                                                                                         >  8:521956/1‑151 (MQ=255)
                                                             gAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGGTCCTGGAGTAGGCTATGGCAAGGTGTTCAGAttt                                                                                       >  6:341136/1‑151 (MQ=255)
                                                                 aCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCGCCTCCCCCCCATACCCATCAGATCATGAGTGATCCTGCATTAGGGTATGGCAAGGTGATGAGATTTtcat                                                                                   >  2:539943/1‑151 (MQ=255)
                                                                     gTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATcaca                                                                               <  1:424159/151‑1 (MQ=255)
                                                                     gTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGGCCCCAAATATCCTTCAGATGATGAGTGATGCTGCAGTAGGGTATGGCCAGGTGAGGAGATTTTGTTcac                                                                                >  8:844747/1‑150 (MQ=255)
                                                                       aaTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGGCTCGTCCCCCAAATAAACTTCAGAGGAAGAGTGATCCGGCATTAGGAGATGGGAAGGTGATCAGATTTTGATAAGAgg                                                                             >  3:941984/1‑151 (MQ=255)
                                                                       aaTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAg                                                                              <  6:1474423/150‑1 (MQ=255)
                                                                        aTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAggg                                                                            <  2:1279643/151‑1 (MQ=255)
                                                                        aTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAggg                                                                            <  5:870379/151‑1 (MQ=255)
                                                                          ccTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgggg                                                                           <  5:1406793/150‑1 (MQ=255)
                                                                          ccTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgggg                                                                           <  8:1291232/150‑1 (MQ=255)
                                                                           cTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGGCCGCCGCCCCCAAAAATCCGTCAGATGATGCGTGATGCTGCCTGTGGGTGTGGCTAGGTGATCAGCTTTTGATCACAGGGGat                                                                         >  8:138013/1‑150 (MQ=255)
                                                                                gTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatg                                                                    <  6:717624/151‑1 (MQ=255)
                                                                                  tCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGGCGCCTCCCCCAAATATACTGGAGAGGATGGGTGTTCCTGCATTAGGCTATGGGGAGGTGATCAGCTTTTGATCACAGGGGAATTatgtt                                                                  >  5:890368/1‑149 (MQ=255)
                                                                                    tGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTGCGAAAGTTATCAGCGGCCGTGCGCCTGCCCCGAATATGCTGCAGATGCTGAGGGGTCGAGCTTTGGGGTAGGGCGAGGGGGTCGGAAGTTGATCACAGGGGCATCatgtagt                                                                >  6:1089837/1‑147 (MQ=255)
                                                                                     gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgat                                                                  <  4:832441/148‑1 (MQ=255)
                                                                                     gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgat                                                                  <  6:1369475/148‑1 (MQ=255)
                                                                                      tAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGGGGCCGCCCCCACAGAGCCGTCAGAGGATGAGTGAGCCTGCATTAGGCGAGGGGAAGGGGATGAGATTTTGATGACAGGGGAGTTTGGATGttt                                                              >  4:55490/1‑150 (MQ=255)
                                                                                       aaGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCGCCGCCCCCAAATATCCTTCAGATGAGGAGTGATGCTGCGGTAGGCTTTGGGAAGGGGATCAGAGTTTGATGACAGGGGAATTATGTTGTTat                                                             >  2:1008086/1‑150 (MQ=255)
                                                                                        aGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGTCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTGATCAAAGGGGATTTATGAttttta                                                             >  3:61866/1‑148 (MQ=255)
                                                                                         cccGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAAt                                                           <  2:968592/150‑1 (MQ=255)
                                                                                           cGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGc                                                         <  2:121295/151‑1 (MQ=255)
                                                                                           cGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATAGACTTCAGATGATGAGTGATGCTGCATTAGCCTAAGGAAAGGTGATAAGATTTTCATCACAGGCGAATTATGATGTTAATTGc                                                         >  6:587726/1‑151 (MQ=255)
                                                                                           cGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGc                                                         <  2:873016/151‑1 (MQ=255)
                                                                                            ggTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTAGCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCa                                                        <  8:115075/151‑1 (MQ=255)
                                                                                             gTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCAt                                                       <  6:739302/151‑1 (MQ=255)
                                                                                                 aggaggAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCa                                                   <  3:683738/151‑1 (MQ=255)
                                                                                                    aggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCt                                                <  1:900956/151‑1 (MQ=255)
                                                                                                    aggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCt                                                <  5:530725/151‑1 (MQ=255)
                                                                                                     ggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTg                                               <  2:384289/151‑1 (MQ=255)
                                                                                                       aaaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGcc                                             <  4:1352381/151‑1 (MQ=255)
                                                                                                       aaaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCGGCCCCCAAAAAAACTTCAGAAGATAACTGATCATGCAGTAGGGTATGGCAAGATTATAAGAATTTAAAAACAGGGTAATAAATATATTAAATGAAttgaaactga                                              >  4:40423/1‑141 (MQ=255)
                                                                                                                  cGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGCTGACTGATCCTGCATTAGGCTATGGGAAGGGGATCAGATTTTGATCACAGGGGAATTATGTTGTTAAATGCATGGGCCCTGCCGGGGCCCCCa                                   >  1:619455/1‑150 (MQ=255)
                                                                                                                   ggCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCAtt                                 <  6:1198602/151‑1 (MQ=255)
                                                                                                                      tATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAATATCCTTAATAAGATGAGTGATCCTGCATTAGTCAATGGGAAGGTGATCAGATTTTGATAACATGGGAATTATGATGTTAAATAAATGGCACCTCACCGAGCCGCCAGTTGt                              >  1:1035827/1‑151 (MQ=255)
                                                                                                                       aTTCCGAAAGTTATCCGCCCCCTTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTg                               <  8:121772/149‑1 (MQ=255)
                                                                                                                       aTTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCGTGGCACCTGCCGGTGCCCCCATTTGt                              <  3:1262844/150‑1 (MQ=255)
                                                                                                                       aTTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGtt                             <  6:280662/151‑1 (MQ=255)
                                                                                                                       aTTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGtt                             <  2:1221929/151‑1 (MQ=255)
                                                                                                                       aTTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGt                              <  5:767210/150‑1 (MQ=255)
                                                                                                                          ccGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTaaa                          <  7:524289/151‑1 (MQ=255)
                                                                                                                             aaaGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCa                                                        <  2:170538/118‑1 (MQ=255)
                                                                                                                             aaaGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaa                       <  2:1173845/151‑1 (MQ=255)
                                                                                                                              aaGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGGGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaa                      <  6:1241542/151‑1 (MQ=255)
                                                                                                                               aGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaag                     <  5:646332/151‑1 (MQ=255)
                                                                                                                                   aTCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCTGTGCCCCCATTTGTTAAAcaaagcaa                  <  8:823348/150‑1 (MQ=255)
                                                                                                                                   aTCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaaa                 <  6:1043718/151‑1 (MQ=255)
                                                                                                                                   aTCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaa                  <  2:687327/150‑1 (MQ=255)
                                                                                                                                   aTCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCGATGGCAAGGTGATCAGATTTTCATGACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaa                  <  4:1021301/150‑1 (MQ=255)
                                                                                                                                      cGCCCCCGTCCTCCTGCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGAt              <  8:192645/151‑1 (MQ=255)
                                                                                                                                       gCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGAt              >  1:759127/1‑150 (MQ=255)
                                                                                                                                        cccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCa            <  8:87589/151‑1 (MQ=255)
                                                                                                                                          cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGATTGATCCTGCATTAGGCTATGGCAAGGTGATGAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAAc          <  8:658754/151‑1 (MQ=255)
                                                                                                                                           ccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGTGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt         <  7:314236/151‑1 (MQ=255)
                                                                                                                                           ccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt         >  3:276212/1‑151 (MQ=255)
                                                                                                                                             gTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGc       >  6:826176/1‑151 (MQ=255)
                                                                                                                                               cctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTc     >  4:744990/1‑151 (MQ=255)
                                                                                                                                                ctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACATGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTtctc     >  1:1212242/1‑150 (MQ=255)
                                                                                                                                                  cctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCa    <  7:1225803/149‑1 (MQ=255)
                                                                                                                                                   ctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCAtt  >  3:506406/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATT  >  NC_000913/422287‑422583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑