Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913423,5520TG21.8% 576.9 / 41.3 262G345G (GGT→GGG) malZmaltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (152/52);  new base G (0/57);  total (153/109)
Fisher's exact test for biased strand distribution p-value = 2.37e-27
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 269 total aligned reads displayed.

GCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATGCCGCCATGAACTATCGTGG  >  NC_000913/423403‑423701
                                                                                                                                                      |                                                                                                                                                     
gCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGt                                                                                                                                                       <  7:1178329/150‑1 (MQ=255)
gCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTg                                                                                                                                                      <  6:241273/151‑1 (MQ=255)
gCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTCGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTg                                                                                                                                                      >  3:689665/1‑151 (MQ=255)
gCCAGCTTGCAGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTTTCGCGGGGAAGACAGTTTTGTCCGCCTCTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGt                                                                                                                                                       <  8:1032596/150‑1 (MQ=255)
 ccAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTgg                                                                                                                                                     >  7:449813/1‑151 (MQ=255)
 ccAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTgg                                                                                                                                                     <  2:1255296/151‑1 (MQ=255)
  cAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTggg                                                                                                                                                    <  8:117009/151‑1 (MQ=255)
  cAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTggg                                                                                                                                                    >  2:105393/1‑151 (MQ=255)
   aGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTgggg                                                                                                                                                   >  4:1473111/1‑151 (MQ=255)
     cTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGgcg                                                                                                                                                 >  4:1394036/1‑151 (MQ=255)
       tGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGAGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGTCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGgcgcg                                                                                                                                               >  6:46658/1‑151 (MQ=255)
             aGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCaata                                                                                                                                          >  6:1374727/1‑150 (MQ=255)
               cTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCaataat                                                                                                                                        >  8:335933/1‑150 (MQ=255)
                gggATTATCAGTCGGAAAGTCTGGTGAATGAAATTTCTCGCGGGGACGACAGTATTGTCCGCCACGGGCTGAACGCGCGGTGTAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGGGGGGCGCGCAATAatat                                                                                                                                      <  8:758657/150‑1 (MQ=255)
                   aTTATCAGTCGGAAAGTCTGGTGAATGAATTTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGCC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATgc                                                                                                                                    <  6:929606/150‑1 (MQ=255)
                     aaTCAGGCGCAAAGGCTGGTCAATGAAATTTATCGCGGGGAACACCGTATTGTCCGCCACTGCCTCAAAGCGCCGTGTAATATGGAC‑GGCTGCCGGGTGCATGTGGGGCATATGCTGGGGGAGGGGGGGGGGGCGCGCAATAATATgcagc                                                                                                                                 <  4:1145480/150‑1 (MQ=255)
                      aTCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATgcagg                                                                                                                                 >  5:1158183/1‑149 (MQ=255)
                      aTCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATgcagc                                                                                                                                 >  8:677084/1‑150 (MQ=255)
                        cAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACg                                                                                                                              >  6:67297/1‑151 (MQ=255)
                        cAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGGCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGGGGGGCGCGCAATAATATGCAGCAc                                                                                                                               <  5:371385/150‑1 (MQ=255)
                             ggaacggcgggTCAATGAAATTAACCGCGGGGAAGACTGAATTGGCCGCCACGGGCGCAACGCGCCGTGAAATATGGCG‑GGCGGGGGGCGGGCGGGGGTGCATAGGCGGGGGGCGGGGGGGGGGGCGCGCAATAATATGCAGCACGTTGcc                                                                                                                         <  6:44051/142‑1 (MQ=255)
                             gcaagggcTGTTGAATTAAATTATTCGCGGGGACGACTGTCTGGACCCCAACTGGCGGAAGGCGCGGGGTATTTTGCAC‑GGCTGGGGGGGGCTGGTGGGGAATATGTTGGGGGAGGGGGGTGGGGCGCGCAATAATATGCAGCACGTTGcc                                                                                                                         <  6:1047424/144‑1 (MQ=255)
                              gAAAGGCTGGTGAATGAAATTAATCGGGGGGAAGACAGTATTGGCCGCCAGTGGCTCAAAGCGCCGTGTATTATGGCC‑GGGGGGCGGCTGGATGTGGTGCATATGCGGGGGGCGGCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCCg                                                                                                                        <  8:505494/151‑1 (MQ=255)
                              caaaGTCTGGTCAATGAAATTTATCGCGGGGAACACAGTATTGCCCCCCACTGGCTTAAAGCGCCGTGTAATAGGGCC‑GGCTGGCGGCGGTAGGGGGGCCATATGCGGGGGGCGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCg                                                                                                                        <  2:33393/150‑1 (MQ=255)
                                aaGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAC‑CGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATCCAGCACGTTCCCggg                                                                                                                      >  1:265115/1‑151 (MQ=255)
                                  gTCTGGTGAATGAAATTTATCGCGGGTAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGAt                                                                                                                    >  5:202823/1‑151 (MQ=255)
                                  gTCTGGTGAATGAAATTTATCGCGCGGAACACACTATTGTACGCCACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGATGCATATGCTGGGGCAGGCGGGTGGGGCGCGCAATAATATGCACAACGTTGCCGGCAt                                                                                                                    >  3:1293137/1‑151 (MQ=255)
                                   tCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCCCGTTGCCGGGATc                                                                                                                   >  7:1304604/1‑151 (MQ=255)
                                   gcTGGTGAATGAAATTTAGCGCGGGGAAGACAGTATTGGCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATc                                                                                                                   <  2:500707/150‑1 (MQ=255)
                                      ggTTAATCAAATTTAGCGGGGGCAACCCAGTATTGTCCGCCAGTGGCTTAAAGCGCCTTGGATTTTGTAC‑GGCTGGCGGCTGGATGTGGTGCATTTGCTGGGGGCGGCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCAc                                                                                                                 <  6:822615/150‑1 (MQ=255)
                                       gTGAATGAAATTTATGGGGGGGAAGCCAGTATTGTCCGCCACTGGCTGAACGCGCCGTGGAATATGGAG‑GGCTGGGGGCTGGTTGGGGTGCATTTGCTGGGGGTGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGTATCACCg                                                                                                               <  3:1100464/151‑1 (MQ=255)
                                         gaatgaaATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGa                                                                                                              <  8:1110181/150‑1 (MQ=255)
                                           atgaaATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAgc                                                                                                           >  2:412922/1‑151 (MQ=255)
                                             gaaATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAgcgg                                                                                                         >  5:1208677/1‑151 (MQ=255)
                                             caaaTTTATCGCGGGAAACCCATTTTTGCCCGCCACTGGCGCAACGCGCGGTGAAATATGGAC‑GGCTGGCGGGTGGTTGGGGTCCATATGCGGGGGGGGGGGGGGGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAgcg                                                                                                          <  5:822191/149‑1 (MQ=255)
                                                     tCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAAGATGCAGCACGTTGCCGGGATCACCGAAGCGGCgaaaga                                                                                                  >  4:1349892/1‑150 (MQ=255)
                                                      gggggggAAGACAGTATTGTCCGCCACTGGCTTAAAGCGCGGGGTAATTTGGCC‑GGTTGGCGGGTGTTTGTGGTGATTATGGTGGGGGTGGCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCgaaagaaa                                                                                                <  1:794106/148‑1 (MQ=255)
                                                      cgggggtAAGCCAGAATTGGCCGCCCGTGCGTCACACCGCCGTGTAATATGGAG‑GGCGGGGGGCTGGTGGGGGGGCAAATGGGGGGGGAGCCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCgaaagaaa                                                                                                <  6:296445/148‑1 (MQ=255)
                                                      cggggggACTACAGTATGGTCCGCCACTGGCTTAACGCGCCG‑GGTATTTGGCCGGGCGGGCGGCTGGTTGTGGGCCATTTGCGGGGGGCGCCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCgaaagaa                                                                                                 <  1:919246/147‑1 (MQ=255)
                                                        cgGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAAcc                                                                                              <  5:165767/151‑1 (MQ=255)
                                                         ggggAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAAccc                                                                                             >  8:1415588/1‑151 (MQ=255)
                                                           ggAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGGGAAAGAAAccc                                                                                             >  7:82149/1‑149 (MQ=255)
                                                           ggAAGACAGTATTGTCCGCCACGGGCTGAACGCGCCGTGGAATATGGAC‑GGCGGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAg                                                                                           <  7:178513/151‑1 (MQ=255)
                                                            gAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCCCCGAAGCGGCGAAAGCAACCCAGc                                                                                          >  5:715089/1‑151 (MQ=255)
                                                            gAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGc                                                                                          >  8:221661/1‑151 (MQ=255)
                                                              acaCAGTATTGGCCGCCACGGGGGTAAACCGCCGGGTAATATGGAC‑GGGGGGGGGGGGGATTGGGGGCATTTGGGGGGGGCGGCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCg                                                                                        <  2:821175/149‑1 (MQ=255)
                                                              aGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCg                                                                                        >  6:372654/1‑151 (MQ=255)
                                                                  aGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGa                                                                                      >  8:626752/1‑149 (MQ=255)
                                                                  aGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGa                                                                                      >  6:554798/1‑149 (MQ=255)
                                                                      ttGTCCGCCACTCGCTGAAAGCGCCGTGTAATATGGAC‑GGCTGGCGGCTGCATGTGGTGCATATGCTGGGGGTGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGt                                                                                 <  8:826785/150‑1 (MQ=255)
                                                                       gggccGCCAGGGGCTTAACGGGCGGTGTAATTTGGCC‑GGGTGGGGGGTGGTTGGGGTGCATTGGCTGGGGGCGGCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGtat                                                                               <  5:957487/148‑1 (MQ=255)
                                                                          cggctacggggttaaaGCGCGGTGTAATAGGTCG‑GGGTGGCGGCTGTATGTGGTGCTTTTGCTGGGGGTGGCGGGTGGGGAGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTatat                                                                             <  7:61309/137‑1 (MQ=255)
                                                                           cGCCACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTg                                                                           >  8:324095/1‑151 (MQ=255)
                                                                            cccAGTGGGTTAACGCGCGGTGTAAAATGGAC‑GGCTGGGGGCTGTTTGGGGGGCATATGGGGGGGGCGGGGGGGGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGt                                                                          <  3:1186057/150‑1 (MQ=255)
                                                                              cACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGGTGGGGGAGGCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCg                                                                        <  5:1263872/151‑1 (MQ=255)
                                                                              cACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCg                                                                        >  7:579659/1‑151 (MQ=255)
                                                                               agtGGTTGAACGCGCCGTGTAATATGGCG‑GGGGGGCGGCTGTTTGTGGGGCATATGCGGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCgg                                                                       <  7:698519/149‑1 (MQ=255)
                                                                                ggggCTGAACGCGCCGTGGAATATGGAC‑GGCGGGCGGCTGGATGTGGTGCATAGGGTGGGGGCGGCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGc                                                                      <  5:1024159/149‑1 (MQ=255)
                                                                                    gtGAACGCGCCGTGGAATAGGGAC‑GGCGGGCGGCGGGTTGTGGGGCATATGCTGGGGGAGGCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAAc                                                                  <  1:1377631/150‑1 (MQ=255)
                                                                                        aaGCGCCGTGGAATATGGCC‑GGCTGGCGGCTGGAGGTGGTGCATATGCTGGGGGCGGCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACAttt                                                              <  1:1027132/151‑1 (MQ=255)
                                                                                        aaGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGACCCCCAGCCGGAAGCGTATATTGTCGGCGAACAttt                                                              >  5:658330/1‑151 (MQ=255)
                                                                                        aaGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACAttt                                                              >  6:1402516/1‑151 (MQ=255)
                                                                                        aaGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGAGGGGGTGCATATGCTGGGGGAGGCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACAttt                                                              <  5:1487269/151‑1 (MQ=255)
                                                                                         aGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAACCCCAGCCGGAAGCGTATATTGTCGGCGAACaggtt                                                             >  4:526036/1‑147 (MQ=255)
                                                                                          gcggggtgtAATTTGGCC‑GGGTGGCGGTTGTATGTGGGCCATATGGTGGGGGCGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTg                                                            <  6:1234011/146‑1 (MQ=255)
                                                                                           cgcCGTGTAATTGGGAC‑GGCTGGCGGCTGGAGGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTgg                                                           <  4:131271/151‑1 (MQ=255)
                                                                                             cggtgtAATATGCAC‑GGCTGGCGGGGGGATTTGGTCAATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGATCATTTTGGCg                                                         <  1:501731/149‑1 (MQ=255)
                                                                                               gtgtattatGGAC‑GGCTGGGGGCTGGATGTGGTGCATATGCTGGGGGAGGGGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGAt                                                       <  3:870615/145‑1 (MQ=255)
                                                                                               gTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGAt                                                       >  3:2691/1‑151 (MQ=255)
                                                                                                gggAATATCGCC‑GGCTGGGGGCTGGATGGGGTGCATATGGTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATg                                                      <  2:1392849/150‑1 (MQ=255)
                                                                                                         gAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAAt                                             >  7:917823/1‑151 (MQ=255)
                                                                                                          aC‑GGCTTGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCAGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCCCGGCAATg                                            >  1:1104599/1‑151 (MQ=255)
                                                                                                                tggcggctggATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTAc                                       >  4:957143/1‑151 (MQ=255)
                                                                                                                tggcggctggATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACAGTTTGGCGATGCACGGCAATGGTTAc                                       >  6:1011093/1‑151 (MQ=255)
                                                                                                                   cggctggATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAgg                                    >  2:1159679/1‑151 (MQ=255)
                                                                                                                     gctggATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGcc                                  >  3:73072/1‑151 (MQ=255)
                                                                                                                      ggggATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCCCCGAAGCGGCGAATGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGcgg                                 >  8:1141296/3‑149 (MQ=255)
                                                                                                                         gATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGAtg                              >  6:491777/1‑151 (MQ=255)
                                                                                                                         gATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAAACCAGCCGGAAGCGTATATTGTAGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGAtg                              >  1:1232088/1‑151 (MQ=255)
                                                                                                                          cggggttccctttGCGGGGGGCGCCGGGGGGGGCGCGCAATAATATGCAGCACGGTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGAtgt                             <  3:936544/139‑1 (MQ=255)
                                                                                                                          aTGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGCCGATGCACGGCAATGGTTACAGGCCGAtgt                             >  1:1486661/1‑151 (MQ=255)
                                                                                                                            gtggtgCATATGGTGGGGGAGGCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTgg                           <  3:140206/151‑1 (MQ=255)
                                                                                                                            gtggtgCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGAtgtcg                           >  3:175095/1‑149 (MQ=255)
                                                                                                                              ggtgCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAAAATTTTGGCGATGCACGGCAATGCTTACAGGCCTATGTGGa                          >  3:58638/1‑150 (MQ=255)
                                                                                                                               gtgCATATGCT‑GGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGa                          >  2:615158/1‑148 (MQ=255)
                                                                                                                               gggcttatGCGGGGGTTGGCTGGTGGGGCGATCAATAATATCCAGCACGTTGGCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCCGCGAACATTTTGGCGATGCACGGCAATGGGTACAGGCCGATGTGGAAg                        <  5:497934/146‑1 (MQ=255)
                                                                                                                                   atatGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATgc                    >  8:292788/1‑151 (MQ=255)
                                                                                                                                     atGCTGTGGGAGGGGGGTGGGGCGCTGAATAATATGCAGCACGTTGCCGGGATCACCGATGCGGCGAAAGAAACCCAGCCGGAAGCTTATATTTTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGACGATGTGGAAGATgcc                   <  1:588484/150‑1 (MQ=255)
                                                                                                                                     atGCTGGGTGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATgccc                  >  7:1396334/1‑150 (MQ=255)
                                                                                                                                     atGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATgccg                  >  5:1019679/1‑151 (MQ=255)
                                                                                                                                      tGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCAAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCAGATGTGGAAGATgccgc                 >  3:1003815/1‑151 (MQ=255)
                                                                                                                                       gCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATgccgcc                >  4:687760/1‑151 (MQ=255)
                                                                                                                                        cTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATgccgcc                >  5:894173/1‑150 (MQ=255)
                                                                                                                                            gggAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATGCCGCCATGa            >  8:148022/1‑150 (MQ=255)
                                                                                                                                               aGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAACCCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATGCCGCCATGAACt         >  4:1031935/1‑150 (MQ=255)
                                                                                                                                                ggCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATGCCGCCATGAACTAt       <  7:28010/151‑1 (MQ=255)
                                                                                                                                                  cGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATGCCGCCATGAACTATCg     >  4:369987/1‑151 (MQ=255)
                                                                                                                                                  cGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATGCCGCCATGAACTATCg     >  3:490958/1‑151 (MQ=255)
                                                                                                                                                  cGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATGCCGCCATGAACTATCg     >  6:88211/1‑151 (MQ=255)
                                                                                                                                                   gggTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATGCCGCCATGAACTATCGt    >  5:578108/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAC‑GGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATGCCGCCATGAACTATCGTGG  >  NC_000913/423403‑423701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑