Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,708,9100GC26.8% 347.3 / 31.5 193A664A (GCG→GCCrsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base G (100/31);  new base C (45/3);  total (153/40)
Fisher's exact test for biased strand distribution p-value = 8.97e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 196 total aligned reads displayed.

CGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCG  >  NC_000913/1708766‑1709060
                                                                                                                                                |                                                                                                                                                        
cGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGCTCCGCGCAAAGCCGCAGTAGCCGCGGCTATTGCCCGCGCCAAAGCACCCaa                                                                                                                                                     >  1:80300/1‑150 (MQ=11)
        gcAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCCGTAGCCGCCGCTATTGCCCGCGCACACGCCCGCAA‑‑Acccgcac                                                                                                                                           >  1:1315884/1‑144 (MQ=14)
          aaaGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTGGATCCGCGCAAAGCGGCCGTGGACGCGGCTATTGCCCGCGCGCACGCCCGAAA‑‑ACgcgcccagc                                                                                                                                        >  2:729585/1‑143 (MQ=14)
           aaGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAGCTGGAACAGCGGAAGGCTAAAGCTGAGCCATAAAATCAGGTAGATCCGCGCAAAGCGGCCGTCGAAGCGGCTATTGAACGAGCCAAAGCGCGGaaggggggagagcg                                                                                                                                         >  2:436794/1‑139 (MQ=2)
            aGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCCCGCAA‑‑CCGCGAACAGCa                                                                                                                                       >  2:1321730/1‑150 (MQ=255)
              ccgccgTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTAGAAGCGGCTATTGCCCGAGCAAAAGCCAACAA‑‑ACACGAACAGCaga                                                                                                                                     >  7:1354979/1‑148 (MQ=14)
                gccgTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCCGTCGCAGCCGCTATTGCCCGCGCGCAAGCCCACAA‑‑ACCCGAACAGCacaagg                                                                                                                                  >  7:867232/1‑146 (MQ=14)
                   gTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCGGCCGTAGACGCCGCTATTGCCCGCGCGCACGCGCGCAA‑‑CCGGGACCAGCACCAGGCt                                                                                                                                >  6:684395/1‑150 (MQ=14)
                   gTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTa                                                                                                                               <  2:1078435/151‑1 (MQ=255)
                    tCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCCGTCGCAGCCGCTATTGCCCCCGCCCACGCCCCCAA‑‑ACCCGACCAGCACCAGGCtc                                                                                                                               >  8:752894/1‑149 (MQ=14)
                    tCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTAGCCGCCGCTATTGCCCGAGCACAAGCCAGCAA‑‑ACCCGAACAGCaaaaggata                                                                                                                               >  1:928948/1‑143 (MQ=14)
                     cGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTAGATCCGCGCAAAGCGGCAGTAGACGCGGCTATAGAACGCAACAGAGACCTCTA‑‑GCGGAAACAGCAACCGGATAAt                                                                                                                             >  3:570231/1‑151 (MQ=2)
                      gAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGACGCCGCTATTGCCCGAGCCCACGCGCGCAA‑‑ACGGGAACAGCACCCGGCTAATg                                                                                                                            >  2:297235/1‑151 (MQ=255)
                      gAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTAGAAGCCGCTATTGCCCGAGCGAAAGCCACCAA‑‑ACGGGACCAGCagaaggataatg                                                                                                                            >  3:433508/1‑140 (MQ=14)
                           cGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCCGTCGCCGCGGCTATTGCCCGCGCGCAGGCCCGAAA‑‑ACGGGGAGAGGAAGCGGCGACTGCGGac                                                                                                                       >  5:1095266/1‑150 (MQ=14)
                           cGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGACGCGGCTATTGCCCGCGCCAACGCCCCCAA‑‑ACCCGCACAGCACCGCGCTAATGCggcc                                                                                                                       >  3:403992/1‑149 (MQ=14)
                             cTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTGGATCCGCGCAAAGCCGCAGTCGCCGCGGCTATTGCCCGCGCCCAGGCGCGCAA‑‑ACGGGACCAGCAGCAGGCTCAAGCGGAggc                                                                                                                     >  2:1116730/1‑149 (MQ=14)
                              tATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTGGATCCGCGCAAAGCGGCAGTGGCCGCGGCTATGGGCCGAGGCCCAGCCGGCTC‑‑ACGGGCACAGCCCCAGGCGAATGCggtaggc                                                                                                                    >  6:1390831/1‑146 (MQ=2)
                              tATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCAGTCGAAGCCGCTATTGCCCGCGCCAAAGCCCGCAA‑‑ACCGGCACAGCAAAAGGCTCATGACGAGca                                                                                                                     >  5:1345154/1‑149 (MQ=11)
                              tATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCCGTAGAAGCGGCTATTGCCCGCGCGAAGGCCAACAA‑‑ACCGGACCAGCAACAgggtgttgacgagcc                                                                                                                     >  1:362189/1‑137 (MQ=11)
                                       tGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTGGATCCGCGCAAAGCCGCAGTAGAAGCCGCTATTGCCCGAGCCAAAGCCAGCAA‑‑ACCCGACCAGCAGCAGGCTAAATCCGAGCCagaagacc                                                                                                             >  6:702895/1‑147 (MQ=11)
                                         ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGCCGCCGCTATTGCCCGCGCCAACGCCAGCAA‑‑ACCCGAACAGCACAAGGCTCAAGCGGAGCCataaaacgaggt                                                                                                         >  6:1233737/1‑143 (MQ=14)
                                             aGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTAGAAGCCGCTATTGCCCGCGCGAAAGCCCGCAA‑‑ACGGGAACAGCACCAGGCTAATGCGGAGCCAGAAGAACAGGTTgg                                                                                                      >  8:537253/1‑149 (MQ=14)
                                               cACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCGCACGCGAGCAA‑‑ACGGGACCAGCACCAGGGTAATGAGGAGCAAGAAGAACAGGttcctac                                                                                                   >  6:999664/1‑146 (MQ=14)
                                                aCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTGGCAGCGGCTATTGCCCGCGCCCAGGCCACCAA‑‑ACCCGACCAGCACCCGGGTATTCACGAGCAAGAAGACGAGGtaggacac                                                                                                  >  8:1040611/1‑144 (MQ=2)
                                                aCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTAGAAGCCGCTATTGCCCGCGCAAAAGCGAGAAA‑‑ACCCGAACAACAACCGAATAAAGACGAGGCAGAAGAAAAGGTTGATcat                                                                                                  >  4:1047318/1‑149 (MQ=14)
                                                  gCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCAGCCGTCGAAGCCGCTATTGCACGAGCCAAAGCACGCAA‑‑GCTGGAGCTCCAAACAGCTACTTCGAAGCAAAAAGAAGAggaaaaacagcg                                                                                                >  1:508489/1‑141 (MQ=11)
                                                   cAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTAGATCCGAGCAAAGAAGACCTACAAGAAGCTATTGACCGTACCAAATAGAGGAA‑‑AAATGAGCAGGAACCGGATAATTAAAAAACAGAAGAACAGGTTGATCCgggc                                                                                               >  7:429252/1‑151 (MQ=2)
                                                   cAAGCTGGAACAGCAACAGGCTAATGCCGGGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTAGAAGCCGCTATTGCCCACCACAAAACACCCAA‑‑GAGTGAACAGAAACAGTATAATTATGAGAAAGAAGAACAGGTTgcacat                                                                                                  >  1:13893/1‑143 (MQ=2)
                                                      gCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCAGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGAGGAGCCAGAAGAACAGTTTGCTACGCGCac                                                                                             >  6:1024350/1‑149 (MQ=17)
                                                         ggAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTAGACGCCGCTATTGCCCGCAGAAAAGAACACAC‑‑TCGTGAACAGAAACAGGCTAATCAAGAGCAATAATAACAGGTGGATCcgctaaaata                                                                                          >  4:1007965/1‑143 (MQ=2)
                                                          gAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCGGCAGTAGCAGCCGCTATTGCCCGCGCAAACGCCAAAAA‑‑ACCCGACCAGCACCCTCCTAATTCGGTGACAGAAGAACAGGTTGCTACTCGCAAAGCTg                                                                                        >  4:688835/1‑151 (MQ=9)
                                                              aGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCAGTCGCAGCCGCTATTGCCCGAGCCAAAGCCACCAA‑‑ACGCGAACAGCACCAGGCTAATCCGGAGCCAGAACAACACGTCCAACCGCGCAAAGCTGc                                                                                       >  8:102438/1‑148 (MQ=14)
                                                                cAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAA‑‑ACGGGACCAGCACCCGGCTAATGCGGAGCCAGAAGAACAGGTTGCTCCGCGCAAAGCTGCAGTCg                                                                                  >  2:363297/1‑151 (MQ=255)
                                                                 aaCAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGtagc                                                                                 >  8:50320/1‑148 (MQ=255)
                                                                 aaCAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCAGCCGTCGAAGCCGCTATTGCCCGAGCAAAAGCGAGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGtagc                                                                                 >  4:1379064/1‑148 (MQ=255)
                                                                  aCAGGCTAATGCCGACGCAGAACAAGTGGCCGATCCACGCAAAGCGGCCGTGGAAGCAGCTATTGCCCGAGCCAAAGCGCGCAA‑‑TCGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTc                                                                                   <  5:1438764/148‑1 (MQ=18)
                                                                    aGGCTAACGCCGAGCCACAACAACAGGTCGATCCCCGCAAAGCGGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCCCGCAA‑‑GCGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGc                                                                              <  2:827028/151‑1 (MQ=14)
                                                                            cccccaccAGAACAACAGGGCGATCCGCGCAAAGCCGCCGTCGAACCCGCTATTGCCCGAGCAAAACGGCCCAA‑‑GCGGGAAGACCACCCGGCTACGGCGGACCCAGAACAACAGGTCGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTg                                                                      <  2:418570/145‑1 (MQ=11)
                                                                             ccGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTAGAAGCGGCTATTGc                                                                     >  5:749303/1‑151 (MQ=255)
                                                                              cGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCGCGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGCGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCAc                                                                   >  2:156216/1‑151 (MQ=255)
                                                                               gctcccgAACACCAGGCCGATCCCCGCAAACCTGCCGTAGAAGCCGCTATCGCCCGAGCCAAAGCGCGCAA‑‑TCGGGAACAGCAACCGGCTAATGCGGAGCCAGAACAACAGGGTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCAc                                                                   <  8:1411487/145‑1 (MQ=255)
                                                                               gAGCCAGAACAACAGGTCGATCCGCGCAAAGCGGCAGTCGAAGCCGCTATTGCCCGCGCCAAAGCCCGCAA‑‑ACCGGAACAGCAACCGGCTAATGCCGAGCCAGAAGAACAGGTTCATCAGCGCAAAGCGGCAGTCGCCGCGGCAATTGCAc                                                                   >  7:1364589/1‑151 (MQ=14)
                                                                                aGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGACCAGGTTGATCCGCGCAAAGCTGCCGAAGACGCGGCTATTGCAcg                                                                  >  6:470759/1‑151 (MQ=255)
                                                                                  ccAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTAGCAGCGGCTATTGCAcgcg                                                                >  1:1115181/1‑151 (MQ=255)
                                                                                   cAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTAGCAGCGGCTATTGCAcgctc                                                               >  6:577383/1‑149 (MQ=255)
                                                                                   cAGAACAACAGGTCGATCCGCGCAAAGCCGCAGTAGAAGCGGCTATTGCCCGCGCCAAAGCCCGAAA‑‑ACGCGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCCGTCGAAGCGGCTATTGCACgcgc                                                               >  1:1281505/1‑151 (MQ=14)
                                                                                    aGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTAGAAGCGGCTATTGCACGCGcc                                                              >  2:219475/1‑151 (MQ=255)
                                                                                     gAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTGGCAGCGGCTATTGCACGCGcc                                                              >  8:1278581/1‑150 (MQ=255)
                                                                                       acaacaGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAAcc                                                                                                                                    >  4:743702/1‑78 (MQ=255)
                                                                                       acaacaGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCCCCCAA‑‑ACGGGAACAGCACCCGGCTAATGCGGAGCCAGAAGACCAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACCCGCCaac                                                           >  2:281416/1‑150 (MQ=255)
                                                                                          acaGGTCGATCCGCGCAAAGCGGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCCCGCAA‑‑ACGGGAACAGCAACAGGCTAATGCGGACCCAGAAGAACAGGTAGCTACGCCCAAAGCTGCGGTAGCACCGGCGAATGCCCgcgcacaccc                                                         >  7:73436/1‑144 (MQ=11)
                                                                                           cagcgcgcaccGCCCAACGCCGCCGTCCCCCCCGCTATCGCCCGCGCCAAAGCGCGCAA‑‑TCTGGAACAGCAACCGGCTAATGCGGACCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCAc                                                       <  2:187017/142‑1 (MQ=14)
                                                                                            aGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTAGCAGCGGCTATTGCACGCGCCAAAGCCCg                                                      >  4:146148/1‑151 (MQ=255)
                                                                                               tCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGACAAAGCACGCAA‑‑GCTGGAACAGCAACAGGCTAATGCAGAGCCAGAAGAACAGGTAGATCCGCGCAAAGCAGCAGTAGAAGCGGCTATTGCCCACGGCACAACACGctc                                                   >  3:122055/1‑149 (MQ=11)
                                                                                                 cccccccgcAAAGCATGCGTCGAAGCCGCTATTGCCCGACCCAAACCGATCAC‑‑GCGTGATGAGCTAAGTCGTTATCTCTAGCCAGAAGAACAGGTCGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGc                                                 <  6:1222715/145‑1 (MQ=9)
                                                                                                  ccccGCGCCACCCCGCCGGCGAAGCCGCTATTGCCCGAGCCAAATCGCTCAA‑‑ACTGGAACAGCAACCCGCTAATCCTGCGGCAGACGACCAGTGAGATCGGCGCAAAGGAGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCt                                                <  5:581109/149‑1 (MQ=255)
                                                                                                   tCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTg                                               >  8:962894/1‑151 (MQ=255)
                                                                                                     cgctctaaGCCGCCGTCGAAGCCGATATTGAAATTGACAAAGCGCGCAA‑‑CGTGTAAAAGAAAATTGCTTATTCTGATGCAGAAGAAGAGGGTGATCCGAGCTAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGa                                             <  4:437405/145‑1 (MQ=2)
                                                                                                     cgcgCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCCACGCACGCAAGCtcg                                              >  6:542994/1‑148 (MQ=255)
                                                                                                      gcgcAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTAGCAGCGGCTATTGCACGCGCGAAAGCACGCAAGCTGGac                                            >  7:71257/1‑150 (MQ=255)
                                                                                                      ccgcAAACCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAA‑‑TCGGCAACAGCAACAGGCTAATGCCGAGCCACAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGaa                                            <  8:340689/150‑1 (MQ=14)
                                                                                                       cgcAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTCGAAc                                           >  1:437302/1‑151 (MQ=255)
                                                                                                       cgaaaaGACGCCGTCGAAGCCGCTATTCCCCGAGCCAAAGCGCGCAA‑‑ACGCGAACAGCACCCTGCTATTGCGGAGGCAGAAGAACAGGTGGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAAc                                           <  2:1102127/148‑1 (MQ=17)
                                                                                                         cccaGCCGCCGGCGCAGCCGCTCTCGCCCGCGCCAAAGCGCGCAA‑‑TCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTTGAGCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAg                                         <  2:1138100/148‑1 (MQ=11)
                                                                                                         cAAAGCCGCCGGCGAAGACTCTATTGCCGAGCCAAAGCGCGCTA‑‑ACGTGAACAGCTACCGTCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGc                                        <  4:156584/151‑1 (MQ=17)
                                                                                                          aaaGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAA‑‑TCGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGcc                                                                                      <  3:201/105‑1 (MQ=255)
                                                                                                          aaaGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAAGATGGGAAGAGAACGGCTAATAACTACACAGAAGAAGAGGTAGATCCGAGCAAAGCGTCCGTCGAAGCAGATATTGCCCGCGCCAACGCGCGCAAgggggggcagc                                        >  5:278259/1‑141 (MQ=2)
                                                                                                          aaaGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGCAGCGGCTATTGCACGCGCCAACGCACGCAAGCTGGAACAGc                                        >  6:317895/1‑151 (MQ=255)
                                                                                                          aaaGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGc                                        >  6:261189/1‑151 (MQ=255)
                                                                                                           aaGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCCCGCAAGCTCGAACAGc                                        >  2:1021162/1‑150 (MQ=255)
                                                                                                             gccgccGTCGCCCCCTCTATTGCCCGTGCCAAAGCTCTTAA‑‑TATTGAACAGCAACCGTCTAATTCGTAGACATAAGAACAGTTTGATCCGCTCAAAGATGCTGTCGATTCGTCTATTGCACGTGCCAAAGCACGCAAGCTGGAACAGCAAc                                     <  4:819530/151‑1 (MQ=2)
                                                                                                             gccgccGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCCACGCCCTCAAGGAGGAACAGCacc                                     >  8:612724/1‑149 (MQ=17)
                                                                                                               cgccgTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAAc                                     >  2:508894/1‑149 (MQ=255)
                                                                                                                gccgTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAgg                                  >  5:1176239/1‑151 (MQ=255)
                                                                                                                 ccgTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGACCAGCACCAGGc                                 >  8:1111584/1‑151 (MQ=255)
                                                                                                                   gTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCACCAGGCt                                >  5:1363073/1‑150 (MQ=255)
                                                                                                                   gTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTCGAACAGCAACAGGCt                                >  3:462674/1‑150 (MQ=255)
                                                                                                                    tCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTCGAAGCGGCTATTGCACGCGCAAAAGCAAGCAAGCAGGAACAGCAACAGGATa                               >  3:1149952/1‑150 (MQ=255)
                                                                                                                     cGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAAt                             >  2:1245998/1‑151 (MQ=255)
                                                                                                                       aaGCCGCTATTGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCCCGCAAGCTGGAACAGCAACAGGGTAATGc                           >  7:213944/1‑151 (MQ=255)
                                                                                                                       aaGCCGCTATTGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTAGAAGCGGCTATTGCACGCGCAAAAGCAAACAAGCAGGAACAGCAACAGGATaaatc                           >  7:1121170/1‑148 (MQ=25)
                                                                                                                         gCCGCTATTGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATcc                                                                                                   <  5:1281799/77‑1 (MQ=255)
                                                                                                                         gCCGCTATTGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTACACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCgg                         >  8:1375022/1‑151 (MQ=255)
                                                                                                                          cctctatgccccGAGGCAACCCGCGCAA‑‑ACTGCACCAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGt                        <  8:404359/142‑1 (MQ=17)
                                                                                                                          ccGCTATTGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCCCGCAAGCTGGACCAGCAACAGGCTAATGCGGt                        >  4:1271039/1‑151 (MQ=255)
                                                                                                                           cGCTATTGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATCCGGt                        >  4:641149/1‑150 (MQ=255)
                                                                                                                            gCTATTGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCAAAGAGGCTATTGCACGCGCAAAAGCAAGAAAGCTCGAACAGCAACAGGATAATCCGGAAc                      >  2:141627/1‑151 (MQ=25)
                                                                                                                              tATTGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTCGAACAGCACCAGGCTAATGCCGTAccc                    >  4:288298/1‑150 (MQ=255)
                                                                                                                               aTTGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTagc                     >  6:41305/1‑147 (MQ=255)
                                                                                                                                ttGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCAGAACTCAAAACCGAAATTGAACGAAACAAAGAACGCAATATTGAACAGCAACAGGCTaaaccgatacaat                   >  1:1234420/1‑139 (MQ=12)
                                                                                                                                  gCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGACGCGGCTATTGCACGCGCAAAAGCACGCAAGCAGGACCAGCAGCAGGCTAATGCGGTagaaga                  >  3:1269707/1‑144 (MQ=25)
                                                                                                                                  gCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCGAACGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCagaa                 >  1:21580/1‑150 (MQ=255)
                                                                                                                                  gCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTCGCAGCGGCTATTGCACGCGCAAAAGCAAGCAAGCTCGCACAGCAGCAGGCTAATGCGGTACAagaa                 >  3:546641/1‑150 (MQ=25)
                                                                                                                                   cccGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAACGCCAGCAAGCTGGCACAGCACCAGGCTatggcccgagcaga                  >  5:225708/1‑135 (MQ=25)
                                                                                                                                     cGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCCCGCGCCAACGCACGCAAGCTGGCACAGCACCAGGCTAATGCGGTACCaga                  >  6:1253383/1‑146 (MQ=255)
                                                                                                                                      gAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAAc             >  2:601928/1‑150 (MQ=255)
                                                                                                                                         ccAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGATAATTCGGTCCAAGAAGAACAGGt         >  4:1179769/1‑151 (MQ=255)
                                                                                                                                                gcgcAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGCTCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAACCAGGAACAGCAACAGGCTAAAGCGCTACCAGAAGAACAGGATGATccc  >  4:255910/1‑150 (MQ=255)
                                                                                                                                                gcgcAA‑‑ACGGGAACAGCAACCCGCGAATGCGCACCCAGAACAACAGGTTGATCCGCCCAAACCGGCCCTAGAAGCGGCAATAGCCCGCGCCAAAGCAAGCAACCGCGCACAGCACCACGGTAAAGCCGTACCCGAACAACACGTTgcacac  >  7:577749/1‑146 (MQ=255)
                                                                                                                                                |                                                                                                                                                        
CGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAA‑‑ACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCG  >  NC_000913/1708766‑1709060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: