Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,818,1450TC26.1% 296.7 / 115.0 178intergenic (‑10/+53)argZ/argYtRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base T (54/65);  new base C (7/35);  total (77/101)
Fisher's exact test for biased strand distribution p-value = 8.52e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.49e-01

Only 100 of 197 total aligned reads displayed.

CTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTG  >  NC_000913/2817995‑2818295
                                                                                                                                                       |                                                                                                                                                        
     gCCTGCGGTTAATTCGGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAatttatt                                                                                                                                                     <  7:1460375/151‑1 (MQ=255)
      ggttcttttAATTACTCTACATATTCTTAACCTTAATTTATGAATATTTAGTATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAacttactt                                                                                                                                                    <  3:908244/144‑1 (MQ=11)
      ggtcattttttttCCTCTAGATCTTGTATACCTTCATTTCTGAATATTTCGTATAAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAacttactt                                                                                                                                                    <  4:671555/140‑1 (MQ=11)
      gcttcttttAATTCCTGTACATCTTCCATACGTTCATTGCTGAATATTTCGGATTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAacttactt                                                                                                                                                    <  1:523739/144‑1 (MQ=14)
      ccTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAGatctgaat                                                                                                                                                    >  1:10457/1‑145 (MQ=255)
      ccTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAagttagtt                                                                                                                                                    >  6:561458/1‑151 (MQ=255)
       gtGCTGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTAtttt                                                                                                                                                   <  1:1108719/150‑1 (MQ=255)
         gCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTATTTTAc                                                                                                                                                 >  8:8355/1‑151 (MQ=255)
         ccGGTTATTTCCGCTACCTCTTCGATACCTTCATTTCTGAAGATTTCGGACAAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTAc                                                                                                                                                 <  1:398872/150‑1 (MQ=14)
          cGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTAct                                                                                                                                                <  2:683314/151‑1 (MQ=255)
          cGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTAct                                                                                                                                                <  2:1433515/151‑1 (MQ=255)
          aggTTAATTCCGCTACCTCTTCCATACCTTCATTGCTGAATATTTCGGATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACttactttact                                                                                                                                                <  4:1221793/150‑1 (MQ=255)
            gTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGTTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAatttatt                                                                                                                                                     <  2:484359/144‑1 (MQ=9)
              aaaTTCCTCTTCCTCTTGGAAACGTTCATAGCTGAATATTTCGGAGTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTActgca                                                                                                                                             <  7:695324/149‑2 (MQ=14)
               aaTACCGCTACCTCTTCGATACCTTCATTGCTGAAGATTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGa                                                                                                                                           <  4:1030096/151‑1 (MQ=255)
                  tactgttcatcttctATACCTTCATATATGAATATTTAGTATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGa‑‑tt                                                                                                                                       <  7:811379/141‑2 (MQ=11)
                  tactgcacatattCCATACCTTCATTCCTCAAGATTTAGGATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGa‑‑tt                                                                                                                                       <  4:520597/139‑2 (MQ=11)
                   cgtcttcatctTCGTTACGTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGa‑‑ttt                                                                                                                                      <  8:167547/142‑3 (MQ=11)
                   ccGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TAtt                                                                                                                                     <  6:66604/151‑1 (MQ=255)
                   ccGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATAGATCGGAAGAGATAAGTTAATGCTAA‑‑TAtt                                                                                                                                     >  7:513616/1‑151 (MQ=11)
                   ccGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACATCCGACAGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTAATTTACTGCAGA‑‑tttt                                                                                                                                     >  4:428156/1‑151 (MQ=14)
                   ccGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTAGAACATCCGACCGCTCGGTTCGTAGCCGAGTACAATATCCACCTGAGCTACGGATCCATCGGGAAACTTAGTTTACTGCAGA‑‑TAtt                                                                                                                                     >  7:359869/1‑151 (MQ=14)
                     gCTACCTCTTCGATACCTTCATTTCTGAAGATTTCTGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGa                                                                                                                                   <  4:616229/151‑1 (MQ=255)
                     gCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActg                                                                                                                                               >  8:900896/1‑141 (MQ=255)
                     gCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAATTAATTTACTGCAGA‑‑TAttt                                                                                                                                    >  1:607711/1‑149 (MQ=255)
                      cTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTAGTTTACTGCAGA‑‑TATTTAt                                                                                                                                  >  6:392580/1‑151 (MQ=255)
                        atctctTCGATACCTTCATTGCTGAAGTTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGAt                                                                                                                                  <  3:60995/147‑1 (MQ=255)
                        acatattCCATACGTTCATTCCTGAAGATTTCGGACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGA‑‑tttttt                                                                                                                                   <  3:649538/143‑3 (MQ=11)
                        aCCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGAt                                                                                                                                  <  8:1375964/149‑1 (MQ=255)
                        aCCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATa                                                                                                                                 <  2:776630/150‑1 (MQ=255)
                         gctctTCGATACCTTCATTGCTGAAGTTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATAcc                                                                                                                               <  2:1213645/150‑1 (MQ=25)
                         ccTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAATTAATTTACTGCAGA‑‑TTTTtatat                                                                                                                                >  1:340473/1‑149 (MQ=14)
                         ccTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCAAACCTCCGACACCTCGGATCGTAGACGAGTACTCTATCCAGCTGAGCTAAGTATGAATCGGGAAATTTATTTTAATTATGA‑‑TATTGATAcc                                                                                                                               >  4:1251076/1‑151 (MQ=14)
                          ctctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTTATAccc                                                                                                                              >  2:1047871/1‑150 (MQ=255)
                          ctctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATACAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCg                                                                                                                              >  7:359838/1‑151 (MQ=255)
                            ctTCGATACCTTCATTGCTGAAGCTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATAc                                                                                                                                <  5:1137712/147‑1 (MQ=255)
                            attCAATACCTTAATTGCTGAATATTTCGGACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGA‑‑ttttttgataccg                                                                                                                            <  6:809559/150‑10 (MQ=11)
                              tccatcccctCATTCCTGAAGATTTCGTACCAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGc                                                                                                                             <  3:170856/141‑1 (MQ=14)
                              tCGATACCTTCATTGCTGAAGTTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGcta                                                                                                                           <  2:209698/150‑1 (MQ=25)
                               cGATACCTTCATTGCTGAAGTTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGctact                                                                                                                         <  6:1359033/151‑1 (MQ=25)
                               cGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGctac                                                                                                                          <  2:262122/150‑1 (MQ=255)
                               cGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTAGTTTACTGCTGA‑‑TATTGagagagctagt                                                                                                                         >  5:1285079/1‑141 (MQ=255)
                                 aTACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAatttattt                                                                                                                                                    >  3:888338/1‑124 (MQ=11)
                                   aCCTTCATTGCTGCAGATTTCGTAGTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTaa                                                                                                                       <  1:1370833/151‑1 (MQ=11)
                                   aCCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTaaa                                                                                                                      <  7:1227133/150‑1 (MQ=255)
                                       taattgaggAAGATATCGGACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGc                                                                                                                    <  5:607168/142‑1 (MQ=14)
                                       tCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTAGTTTACTGCTGA‑‑TTTTGTGAGCGCTAGTAAAGcg                                                                                                                   >  2:592489/1‑148 (MQ=14)
                                       tCATTGCTGAAGATTTAGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGAAGATTTTTTGATACCGCTACTAAAGc                                                                                                                    <  5:954480/150‑1 (MQ=11)
                                        cATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTTAAGCCat                                                                                                                 <  5:1334252/150‑1 (MQ=255)
                                        cATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCat                                                                                                                 <  6:1134093/150‑1 (MQ=255)
                                           ttatGAATATTTCGTATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGctac                                                                                                                          <  1:8254/137‑1 (MQ=14)
                                           tGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTAGTTTACTGCTGA‑‑TAttttgatagctatta                                                                                                                        >  5:8257/1‑127 (MQ=14)
                                           agCTGAATATATAGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCgta                                                                                                                <  8:429422/149‑1 (MQ=14)
                                            gCTGAAGTTTACGTATAATATGGTGCATCCGGCAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGAATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCaa                                                                                                            <  4:1206896/151‑1 (MQ=11)
                                             cggAATATCTCGTATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCa                                                                                                             <  6:358349/149‑1 (MQ=14)
                                             cgaaattttttGGAGAAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATc                                                                                                              <  3:1104272/143‑1 (MQ=14)
                                               gAAGATTTCGGAGAATATGGTGGATCCGGGTGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGGTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGt                                                                                                          <  8:1095650/150‑1 (MQ=255)
                                               gAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGt                                                                                                          <  7:1340998/150‑1 (MQ=255)
                                                aaGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTaa                                                                                                        <  6:1224566/151‑1 (MQ=255)
                                                   aTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAATTAATTTACTGCTGA‑‑TATTTATAAAGCTAATATAGCAATATAAAgaatcat                                                                                                     >  3:979488/1‑145 (MQ=11)
                                                     ttCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAgaga                                                                                                    <  1:121504/150‑1 (MQ=255)
                                                     ttCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAgaga                                                                                                    <  2:1091972/150‑1 (MQ=255)
                                                      tCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGAt                                                                                                   <  8:971427/150‑1 (MQ=40)
                                                       cGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACAGCTGA‑‑AAttt                                                                                                                                    >  1:1297753/1‑115 (MQ=255)
                                                       cGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTAGTTTACTGCTGA‑‑TATTGATAGCGCTAGTAAAGCCATATCAAGTAag                                                                                                       >  6:974550/1‑145 (MQ=255)
                                                                    gCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTAGTAAAGCCATATCAAGTAAGAGATGTTGCATCCGggagg                                                                                    >  2:329864/1‑151 (MQ=255)
                                                                    gCATCCGGGAGGATTCGAACCTCAGACCGCACGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTAGTTTACTGCTGA‑‑TTTTGATAACCCTAGTATAGACATATAAAGTATAAGAtattgaatagcggagg                                                                                    >  3:732495/1‑136 (MQ=14)
                                                                     cATCCGGGAGGATTCGAACCTCCGCCCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTCCGGATGCATCGGGAAAGTTATTTTACTGCTGA‑‑TATTGAGACAGCTAGTAACGCCATATCAAGTAAGAGATGGTGCATCAGggaggc                                                                                   >  2:55386/1‑150 (MQ=11)
                                                                      aTCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGAt                                                                                  <  6:358323/151‑1 (MQ=39)
                                                                      aTCCGGGAGGATTCGAACCTCCGACCGCTCGGTGCGTAGCCGAGTATTCTTTATATATGATCTTCGTATCCATGGGTTAATTTTTTTTACTTATTA‑‑TATTGATAACGCTACTTAAGCAATATCAATTAAGAGATGGTGCATCCGGGAGGAt                                                                                  <  3:400959/151‑1 (MQ=2)
                                                                         cGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGt                                                                                                          <  7:638640/124‑1 (MQ=40)
                                                                         cGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAATTATTTTACTGCTGA‑‑TATTTATAAAGCTAATAAAGACATATAAAATAAGAGATGTTGAAtaaagaacgatac                                                                                >  7:1116270/1‑138 (MQ=11)
                                                                           ggtggaTTCTAACCTCCGACCGGGCGGTTCGTAGCCGAGTACTCTATCCAGCTGTGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGa                                                                              <  5:301457/147‑1 (MQ=255)
                                                                            gaggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTTATACCGCTAATAAAGCCATATCAAGTAAGAGATGTTGAATCAGCGAGGAttatac                                                                             >  4:581407/1‑146 (MQ=14)
                                                                             aggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgct                                                                                                                                             >  3:1428551/1‑87 (MQ=38)
                                                                                aTTCGAACCTCCGACCGCTCGGGTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTcc                                                                        <  2:525555/151‑1 (MQ=255)
                                                                                aTTCAAACCTCCTACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGGTACGGATGCATAGGGAATTTTTTTTTACTTCTGA‑‑TATTGATACCGCTAATACAGCCATATCAAGTACCAGATGGTGCATCCGGGAGGATTCGAACCTcc                                                                        <  2:1249852/151‑1 (MQ=14)
                                                                                  tCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGa‑‑t                                                                                                                                        >  1:1094624/1‑85 (MQ=40)
                                                                                      aCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTAGTTTACTGCTGA‑‑TTTTGATAGCGCTAGTAAAGAGATATCTAGTAAGAGATGGTGCCTCCGGGAGGATTAGacgcgccgagcgg                                                                  >  6:726512/1‑139 (MQ=11)
                                                                                      aCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTAGTTTACTGCTGA‑‑TATTGATAGAGCTAGTCAAGCCATATCTAGTAAGAGATCTTGCAGCCGCGAGGATTAGacagagcgacagc                                                                  >  8:338702/1‑139 (MQ=11)
                                                                                            gACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTTATAGCGCTACTAAAGCCATCTCAAGTAAGAGATGTTGACTCCGCGAGGATTAGACTCGCCGAgcgcctcgcg                                                            >  5:1243104/1‑145 (MQ=14)
                                                                                             aCCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGAAAGAGATGGTGCATCCGGGAGGATTCGACGCGCCGACCGccacgctc                                                           >  8:986541/1‑144 (MQ=14)
                                                                                                    ggTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActg                                                                                                                                               >  7:949752/1‑62 (MQ=39)
                                                                                                              cGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGACCCTCCGACCGACCCCGTCCTAGCCGAGTActcaa                                           >  2:616993/1‑148 (MQ=17)
                                                                                                                   aCTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTATAATGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGTTGCATCCGGGAGGATTCCACCCACGGCCCCTCCGGTTCGTGGCCGattcaaaaaaaac                                       >  7:1153283/1‑138 (MQ=11)
                                                                                                                        aTCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TAtt                                                                                                                                     <  8:1015954/50‑1 (MQ=255)
                                                                                                                        aTCCAGCTGAGCTACGGATGCATCGGGAAATTTAATTTACTTCTGA‑‑TAtt                                                                                                                                     >  3:1019934/1‑50 (MQ=17)
                                                                                                                         tCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTAGTAGCCGAGTACACATACCAGCTGacc                                >  5:1423028/1‑148 (MQ=21)
                                                                                                                          ccAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTTA‑‑TATTGATACACCTACTAAAGAAATATCAAGTAAGAGATGTTGCATCCGGGAGGATTCGAACCTCAGAAAGATCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCt                               >  4:836905/1‑150 (MQ=21)
                                                                                                                                      cGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCaaatg                  >  4:181565/1‑147 (MQ=255)
                                                                                                                                      acgtctgctcttcgtaaTTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATc                    <  5:1054199/135‑1 (MQ=21)
                                                                                                                                       ggATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCaaatgg                 >  6:1090559/1‑146 (MQ=255)
                                                                                                                                         aTGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTACACCCGAAGACCCAACGGATAATATCGGAATACACTATACATCTGAGATACggatggatatgga                >  4:707366/1‑145 (MQ=9)
                                                                                                                                            cATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCaaggtggtaat            >  8:1206761/1‑141 (MQ=255)
                                                                                                                                             aTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTCGAAGCTCAGACCCATCGCTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAact            >  3:571376/1‑151 (MQ=18)
                                                                                                                                               ggggaaaaCTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAACGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAactta          <  8:568858/146‑1 (MQ=255)
                                                                                                                                                   aaaaGTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACttacttt      <  6:1149595/150‑1 (MQ=255)
                                                                                                                                                    aaaCTTATTGTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTg                                   <  6:700216/121‑1 (MQ=255)
                                                                                                                                                      atttatttTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACttactttact   <  2:836338/151‑1 (MQ=255)
                                                                                                                                                       tttatttTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATAGGGATccttacatgcgtg  >  8:237558/1‑144 (MQ=255)
                                                                                                                                                       |                                                                                                                                                        
CTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTG  >  NC_000913/2817995‑2818295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: