Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,818,1580TA23.2% 302.4 / 83.8 169intergenic (‑23/+40)argZ/argYtRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base T (62/67);  new base A (12/27);  total (75/94)
Fisher's exact test for biased strand distribution p-value = 6.67e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01

Only 100 of 179 total aligned reads displayed.

AATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGC  >  NC_000913/2818010‑2818296
                                                                                                                                                     |                                                                                                                                            
   tactgttcatcttctATACCTTCATATATGAATATTTAGTATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGa‑‑tt                                                                                                                                        <  7:811379/141‑2 (MQ=11)
   accGCTATCTCTTCGATACCTTCATTGCTGAAGTTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑tat                                                                                                                                       <  4:649598/150‑1 (MQ=12)
    tctcTACCTCTTCGAACCCTTCATTGCTGAAGATTTCGTACTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGa‑‑ttt                                                                                                                                       <  5:1247775/147‑3 (MQ=14)
    cgtcttcatctTCGTTACGTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGa‑‑ttt                                                                                                                                       <  8:167547/142‑3 (MQ=11)
    ccGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TAtt                                                                                                                                      <  6:66604/151‑1 (MQ=255)
    ccGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑TAtt                                                                                                                                      >  7:359866/1‑151 (MQ=255)
     cGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTg                                                                                                                                     <  8:1101665/151‑1 (MQ=255)
      gCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGa                                                                                                                                    <  4:836861/151‑1 (MQ=255)
       gcaCCTATTGGATCCCTTCATAGAGGAATATTTCGGACAATATGGTGCATCCGGGATGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑ttttt                                                                                                                                     <  2:427526/147‑2 (MQ=11)
       cTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTAGTTTACTGCAGA‑‑TATTTAt                                                                                                                                   >  6:392580/1‑151 (MQ=255)
       cTACATCTTCCAAACGTTCCTTTCTGCAGATTTAGTATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑ttttt                                                                                                                                     <  3:427032/149‑2 (MQ=11)
         atctctTCGATACCTTCATTGCTGAAGTTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGAt                                                                                                                                   <  3:60995/147‑1 (MQ=255)
         aCCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGAt                                                                                                                                   <  8:1375964/149‑1 (MQ=255)
         aCCTATTCGATCCCTTCATTCCTCAATATTTCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑ttttttga                                                                                                                                  <  7:137024/150‑5 (MQ=11)
           ctctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTTATAccc                                                                                                                               >  2:1047871/1‑150 (MQ=255)
           ctctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATACAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCg                                                                                                                               >  7:359838/1‑151 (MQ=255)
             attCAATACCTTAATTGCTGAATATTTCGGACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑ttttttgataccg                                                                                                                             <  6:809559/150‑10 (MQ=11)
                cGATACCTTCATTGCTGAAGTTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGctac                                                                                                                           <  4:1490725/150‑1 (MQ=25)
                  aaaCCTTACTTCCTGAATATGTAGTACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGctact                                                                                                                          <  2:867313/149‑1 (MQ=14)
                  aTAACTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCTCTCGGTTCTTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTaa                                                                                                                        <  2:676356/151‑1 (MQ=255)
                    aCCTTCATTGCTGCAGATTTCGTAGTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGCTACTaa                                                                                                                        <  1:1370833/151‑1 (MQ=11)
                      gttaaTTTCTGAATATTTCGTATTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAg                                                                                                                      <  7:394558/147‑1 (MQ=14)
                      cTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTAATTTACTGCAGA‑‑TTTTtataaacctaaaaaaaac                                                                                                                    >  7:1250573/1‑137 (MQ=14)
                        taattgaggAAGATATCGGACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGc                                                                                                                     <  5:607168/142‑1 (MQ=14)
                        taaTTGATGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGc                                                                                                                     <  1:653523/148‑1 (MQ=11)
                        tCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTAGTTTACTGCTGA‑‑TTTTGTGAGCGCTAGTAAAGcg                                                                                                                    >  2:592489/1‑148 (MQ=14)
                        tCATTGCTGAAGATTTAGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGAAGATTTTTTGATACCGCTACTAAAGc                                                                                                                     <  5:954480/150‑1 (MQ=11)
                         cATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTTAAGCCat                                                                                                                  <  5:1334252/150‑1 (MQ=255)
                         aatttatGAATATATCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGcc                                                                                                                    <  3:8338/144‑1 (MQ=14)
                         aaTTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGTGCGCTCGGTTCGTAGCCGAGTACTCTATCCTGCTGAGCTACGGATGCATCGTG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCata                                                                                                                 <  3:1464418/150‑1 (MQ=255)
                          atatctcaaGATCTAGGACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGccgt                                                                                                                  <  7:560934/144‑3 (MQ=14)
                          aTTGCTGAAGATTTCTGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCatat                                                                                                                <  1:306031/151‑1 (MQ=255)
                            ttatGAATATTTCGTATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGctac                                                                                                                           <  1:8254/137‑1 (MQ=14)
                            agCTGAATATATAGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCgta                                                                                                                 <  8:429422/149‑1 (MQ=14)
                             gCTGAAGTTTACGTATAATATGGTGCATCCGGCAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGAATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCaa                                                                                                             <  4:1206896/151‑1 (MQ=11)
                              cggAATATCTCGTATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCa                                                                                                              <  6:358349/149‑1 (MQ=14)
                              cgaaattttttGGAGAAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATc                                                                                                               <  3:1104272/143‑1 (MQ=14)
                               tGAATATTTCGTATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCa                                                                                                              <  4:602896/150‑1 (MQ=14)
                                gAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTa                                                                                                          <  7:1223542/151‑1 (MQ=255)
                                    aTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTAATTTACTGCTGA‑‑TATTTATAAAGCTAATATAGCAATATAAAgaatcat                                                                                                      >  3:979488/1‑145 (MQ=11)
                                      ttCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAgaga                                                                                                     <  1:121504/150‑1 (MQ=255)
                                      ttCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAgaga                                                                                                     <  2:1091972/150‑1 (MQ=255)
                                       tCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGAt                                                                                                    <  8:971427/150‑1 (MQ=40)
                                        cGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACAGCTGA‑‑AAttt                                                                                                                                     >  1:1297753/1‑115 (MQ=255)
                                        cGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTAGTTTACTGCTGA‑‑TATTGATAGCGCTAGTAAAGCCATATCAAGTAag                                                                                                        >  6:974550/1‑145 (MQ=255)
                                        cGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCAGAGCGCTAGCGACGTAGCCGCGTCGACTGTCAAGCTGAGCTACGGAGGCATCCGG‑AAATTTATTTTACTCATCA‑‑TATTGAGCCCGCTCCGACCGCGATATCAAGTACGAGATgg                                                                                                  >  6:1138671/1‑151 (MQ=9)
                                                     gCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTAGTAAAGCCATATCAACTAAGAGATGGTGCATCCGggtgg                                                                                     >  2:322269/1‑151 (MQ=255)
                                                      cATCCGGGAGGATTCGAACCTCCGCCCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTCCGGATGCATCGGG‑AAAGTTATTTTACTGCTGA‑‑TATTGAGACAGCTAGTAACGCCATATCAAGTAAGAGATGGTGCATCAGggaggc                                                                                    >  2:55386/1‑150 (MQ=11)
                                                          cGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGt                                                                                                           >  6:636099/1‑124 (MQ=40)
                                                          cGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGt                                                                                                           <  7:638640/124‑1 (MQ=40)
                                                          cGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTAGTTTACTGCTGA‑‑TATTTATAGCGCTACTAAAGCCATATCAAGTAAGAGAGGGTGCATCCGGGAGGAtt                                                                                  >  6:444279/1‑149 (MQ=255)
                                                          cGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTATTTTACTGCTGA‑‑TATTTATAAAGCTAATAAAGACATATAAAATAAGAGATGTTGAAtaaagaacgatac                                                                                 >  7:1116270/1‑138 (MQ=11)
                                                           gggAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTg                                                                                                                                     >  7:801212/1‑97 (MQ=255)
                                                            ggtggaTTCTAACCTCCGACCGGGCGGTTCGTAGCCGAGTACTCTATCCAGCTGTGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGa                                                                               <  5:301457/147‑1 (MQ=255)
                                                                 aTTCGATCCTGCGACGGCTCGGTTCGTATCGGAGTAGTCTATCCAGCTGATCTACGTACGCATCGGG‑AAATTTATTTTACTGGTGA‑‑TATTGATACCGCTACTACATCCATATCAAGTAATAGATGGTGCATCCGGGAGGATTCGAACCTcc                                                                         <  5:681444/151‑1 (MQ=14)
                                                                 aTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGaagcgcc                                                                         >  2:178225/1‑146 (MQ=255)
                                                                 aTTCGAACCTCCGACCGCTCGGGTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTcc                                                                         <  2:525555/151‑1 (MQ=255)
                                                                  ttCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATAACGCTACTAAAGCCATATCAAGTAAGAGATGGTGAATCAGGGAGGATTAGacgcgccg                                                                        >  6:719263/1‑144 (MQ=14)
                                                                   tCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑t                                                                                                                                         <  5:1093635/85‑1 (MQ=40)
                                                                   tCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑t                                                                                                                                         >  1:1106018/1‑85 (MQ=40)
                                                                   tCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑t                                                                                                                                         >  1:1094624/1‑85 (MQ=40)
                                                                       aCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTAGTTTACTGCTGA‑‑TTTTGATAGCGCTAGTAAAGAGATATCTAGTAAGAGATGGTGCCTCCGGGAGGATTAGacgcgccgagcgg                                                                   >  6:726512/1‑139 (MQ=11)
                                                                       aCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTAGTTTACTGCTGA‑‑TATTGATAGAGCTAGTCAAGCCATATCTAGTAAGAGATCTTGCAGCCGCGAGGATTAGacagagcgacagc                                                                   >  8:338702/1‑139 (MQ=11)
                                                                        ccTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGAt                                                                                                                                   <  2:700988/86‑1 (MQ=255)
                                                                        ccTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGTATCGaaagaccgcccggg                                                                  >  2:261414/1‑139 (MQ=14)
                                                                        ccTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCAGCGAGGATTAGacgcgaagagcctc                                                                  >  7:941464/1‑138 (MQ=14)
                                                                        ccTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCAGA‑‑TATTGAt                                                                                                                                   >  4:701695/1‑86 (MQ=255)
                                                                             gACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTTATAGCGCTACTAAAGCCATCTCAAGTAAGAGATGTTGACTCCGCGAGGATTAGACTCGCCGAgcgcctcgcg                                                             >  5:1243104/1‑145 (MQ=14)
                                                                             gACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGtt                                                             >  2:1235803/1‑151 (MQ=255)
                                                                              aCCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTAGTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGACGCTCCGACCGcgacgatc                                                            >  2:829025/1‑144 (MQ=255)
                                                                              aCCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGAAAGAGATGGTGCATCCGGGAGGATTCGACGCGCCGACCGccacgctc                                                            >  8:986541/1‑144 (MQ=14)
                                                                                  cTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa                                                                                                                                            >  3:705488/1‑69 (MQ=255)
                                                                                  cTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTAGTTTACTGCTGA‑‑TATTGATACCCCTAGTAAAGAGATATCAAGTAAGAGATCGTGAATCACCGAGGATTAGACGCGCCGACCgtcgcgctcgtcg                                                        >  8:1090615/1‑139 (MQ=14)
                                                                                    cGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAg                                                        <  4:1237039/149‑1 (MQ=255)
                                                                                          gTAGCCGAGTACTCTATCCAGCTGAGCTACGTATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAATAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTAc                                                <  8:474447/151‑1 (MQ=255)
                                                                                                  gTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGAACCAGCGCCCGCGCCGAAGTTAGCCGAGTAgtcacgac                                         >  2:1353294/1‑145 (MQ=14)
                                                                                                   tACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑tat                                                                                                                                       <  3:251102/55‑1 (MQ=255)
                                                                                                    aCTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTATAATGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGTTGCATCCGGGAGGATTCCACCCACGGCCCCTCCGGTTCGTGGCCGattcaaaaaaaac                                        >  7:1153283/1‑138 (MQ=11)
                                                                                                       ctATCCAGCTGAGCAACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTaagatctcaagagc                                    >  8:1284904/1‑137 (MQ=21)
                                                                                                         aTCCAGCTGAGCTACGGATGCATCGGG‑AAATTTAATTTACTTCTGA‑‑TAtt                                                                                                                                      >  3:1019934/1‑50 (MQ=17)
                                                                                                          tCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTAGTAGCCGAGTACACATACCAGCTGacc                                 >  5:1423028/1‑148 (MQ=21)
                                                                                                              gCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCACCGATCGTAGCCGAGTACTATATCCAGCTGAGCTaac                             >  3:600041/1‑148 (MQ=255)
                                                                                                                   gCTTCGTATGCATCGCG‑ATATTTAATTTACTGTAGA‑‑TATTTTAAACGCTACTATATCCATATTTAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGc                        <  4:569879/150‑1 (MQ=17)
                                                                                                                        ggATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCaaatgg                  >  6:1090559/1‑146 (MQ=255)
                                                                                                                          aTGCATTGGGAAAACTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCt                                     <  3:132454/131‑1 (MQ=18)
                                                                                                                          aTGCATCGGGAAAACTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGaa                                                                              <  1:590598/90‑1 (MQ=255)
                                                                                                                              aTCGCG‑AGATTTAATTTACTCATGC‑‑TCTTCTTTACGTTACTAGCGGCATATTACGTACTAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAactt            <  6:337624/151‑1 (MQ=11)
                                                                                                                                 ggg‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTACAACCTAAGCCCGAACCCATAGTAGCCGATTACTCTATACAGGTGAGCTACGTATGCAAAGGGAAaattac          >  4:51645/1‑150 (MQ=17)
                                                                                                                                   ttacatttaattTTCTCTTCA‑‑TATTTATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAactt            <  1:386742/143‑1 (MQ=18)
                                                                                                                                   gcttatttttttTACTCATGA‑‑TATTCTTACCGTTCCTTCTTCCATATTTAGTAATATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAacttactt        <  4:948640/147‑1 (MQ=14)
                                                                                                                                   g‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCAGACCGATCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACttacttt       >  4:1079371/1‑151 (MQ=255)
                                                                                                                                    aaaaGTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACttacttt       <  6:1149595/150‑1 (MQ=255)
                                                                                                                                      tgttttttttACTGTTGA‑‑TATTGATACCGCTACTATAGCAATATATATTAATATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACttacttta      <  4:392736/148‑1 (MQ=17)
                                                                                                                                        tttatttTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATAGGGATccttacatgcgtg   >  8:237558/1‑144 (MQ=255)
                                                                                                                                          ttattttttgctgA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATACATCGGAAAACATACTTTAATga  >  4:328224/9‑149 (MQ=21)
                                                                                                                                           atttTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGAACCTCAGAGCGCACGGGTGGTAGCCGATTACTCTATCCAGGTTAGCTACGGGTGGATGGGGATACTTACTTTACTGc  >  8:727089/1‑149 (MQ=21)
                                                                                                                                              ctACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCt                                     <  1:741948/110‑1 (MQ=255)
                                                                                                                                                   gctgA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATcc                                                                                           <  6:1345234/52‑1 (MQ=35)
                                                                                                                                                    ctgA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCCAACGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGAt                          >  2:609331/1‑116 (MQ=255)
                                                                                                                                                    ctgA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGAt                                                                                   <  6:690657/59‑1 (MQ=35)
                                                                                                                                                     |                                                                                                                                            
AATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGC  >  NC_000913/2818010‑2818296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑