Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,270,1670CG22.4% 296.3 / 32.6 196intergenic (+565/+458)yhaC/garKpentapetide repeats‑related protein/glycerate kinase I
Reads supporting (aligned to +/- strand):  ref base C (77/51);  new base G (36/1);  total (136/60)
Fisher's exact test for biased strand distribution p-value = 4.05e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 198 total aligned reads displayed.

TCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGC  >  NC_000913/3270032‑3270317
                                                                                                                                       |                                                                                                                                                       
 cGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGACGTGGCCAGTCCTTAATGTTTCGCCCGCGCCaaaacc                                                                                                                                          >  6:1070436/1‑148 (MQ=11)
           cattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAATTTTTTTTTTAAGTAAACCAGGTGAAACTGAACGATAATCCGCTTTCTTTTTGGTATAGAGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgcc                                                                                                                               <  4:326112/151‑1 (MQ=11)
               ccgctcccccccccccCAAACGCAAACACCCGCCGACGCGGGTTTTTACGTATTTTTTTTTAAACTGCCCTATAAGCAGATTTCTTTTTTTTATTTCTTTGTTTACATTCATTAATCTTTTCCCAGCTCCAAAAGCAAAAACCCGCCGAAg                                                                                                                           <  1:346802/141‑1 (MQ=2)
                    ttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGGGTTCTGTGGTGTAGAGCGTGGTGGCGAGTCCTTCAAGCTGTGGCTCCGTCAAAAACAAAAACCCGCCGCAggcggg                                                                                                                      >  6:1256165/1‑146 (MQ=2)
                      cTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCGAGGCATTCATCGTTGTGCCCCTCCAAAAGCAAAAACCCGCCGCAG‑CGGGgtt                                                                                                                   >  8:1146464/1‑151 (MQ=11)
                      agcgcacTCCACAAGCAGAAACCCGCCGCACCGGGTTTTTACGTAAAGCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgcc                                                                                                                               <  6:1243565/134‑1 (MQ=11)
                        gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTAGAGCGTCGTGGCCAGGGCGGCATCGTTGGGCGGCAGCAAAAGCAAAACACCGCCGCAG‑Cgggggtgg                                                                                                                 >  2:38387/1‑146 (MQ=2)
                        ctcactcaacaaGCACAAACCCGCCGCACCGGGTTATTACGTAAATCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGttt                                                                                                                   <  5:1458353/139‑1 (MQ=11)
                        cccccTCCACAAGCACAAACCCGCCGTACCGGTTTATTACGTACAGCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGttttt                                                                                                                 <  8:1301214/150‑1 (MQ=11)
                         ccccTCCACAAGCACAAACCCGCCGCACCGGGTTTTTACGTACACCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTa                                                                                                                <  2:409629/151‑1 (MQ=11)
                          cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGAGGCGAGGCGGTCATGGGTGGCCCCCAGCAAAACCAATAACCCGCCGCAG‑Tggggtggtt                                                                                                                >  8:1391766/1‑144 (MQ=2)
                          cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTGGGGTATAGCGTCTTGGAGAGGCAATCATAATTCAGCACCAACAAAAACAAAAACccgcagcagagcggtaata                                                                                                                 >  1:449422/1‑135 (MQ=11)
                              ccAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGGATAGCGTCGTGGACCGGCCTTCAGCGTGCGGCGCCGCCCAACCCCAAAACCCGCCGCAG‑Ccgggggtgacggt                                                                                                            >  8:1271215/1‑140 (MQ=2)
                               caaccccacacaCCCGCCGAATCGGTTTTTTACGTAAACCCCCTGACAGTGACCCATACGCCCCTGTCTTTCCGCCCTCGGCTCTTCCCCACTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTaaa                                                                                                          <  8:344753/140‑1 (MQ=2)
                                aaaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACCGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTAATATTTCTAACCCTCCAAAATCAAAAACCCCCCGAAG‑CGGGTTTTTACGTAAAt                                                                                                         >  3:336110/1‑151 (MQ=11)
                                aaaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACCGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGTCATTCATATTTATTCACCTACAAAAGCAAAAACCCGCCGAAG‑ACGGTTTTTACGTAAAt                                                                                                         >  3:343090/1‑151 (MQ=11)
                                   aGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGTGGAGAGCGACGGGGAGCGTCATTCATGGGGGCGCGCCGGCAACACCAAAAACCCGCCGCAG‑gggggggtggcggagaag                                                                                                        >  2:1407677/1‑135 (MQ=2)
                                       aaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCAAGTCAATCATAATTATGCCCCACCAAAAGCAAAAACCCGCCGAAG‑CCGGTGTTTACGGAAATCAAGTg                                                                                                   >  3:821278/1‑150 (MQ=11)
                                          aCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAAc                                                                                               <  2:1484699/151‑1 (MQ=35)
                                           ccccccGCACCGGGTTATTACGTACACCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACt                                                                                              <  8:37293/151‑1 (MQ=11)
                                            ccgccgACCCGGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCGTATCGGCCTAGTGGCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTg                                                                                             <  5:434940/151‑1 (MQ=11)
                                            ccgcccAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATACGCCGCTTGCTTTTGGCTATAGCGTCGGGCCCTGTCATTCCTCCTTCTCCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTg                                                                                             <  2:1089236/151‑1 (MQ=11)
                                             cgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTAAAAGTTATGCCCCGCCAAAACCAAAAACCCGCCGCAG‑GCGGGTTTTACGTTAATCACGTGaaaattt                                                                                            >  5:53381/1‑147 (MQ=11)
                                                 gtaccGGGTTTATACGTAAATCATTTTAAACTGAAAGATAAGCCGCTTTCTTTTTTGTATATAGTCTTGGACATTCATTAATATTTCTGTCTTTCGAAATTCAAAAACCCGCCGAAG‑CGGTTTTTTACGTAAATCAGGTGAAACTGACCGa                                                                                        <  7:686337/147‑1 (MQ=255)
                                                    gCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGTGTATAGAGTCGTGTAAAGTCAATCAAAATAAAACACCAACAAAAACAAAAACCCGCATAAG‑AAGGGTTATTCGTAAATGAAGTGaaaattgacgata                                                                                      >  7:966398/1‑140 (MQ=2)
                                                      gggTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGGAAGGCAATCATGTTTTGTCCCCTCCAAAAACAAAAACCCGCAGAAGACCGTTATTTAC‑TAAATCAAGGGAAAATTCCCGATaatc                                                                                   >  4:536360/1‑149 (MQ=2)
                                                       ggTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATGGTTGGGGGCCGGCAAAAGCAAAAACCCGCCGCAG‑GGGGGTGTTAAGGAAATCAGGGGAAACTGGCCGGTGAGcc                                                                                  >  4:1139283/1‑151 (MQ=11)
                                                       ggTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGGCCTTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGCAG‑GGGGTTGTTACGTAAATCAGGGGCAACTGCCCGATAATcc                                                                                  >  6:1097795/1‑151 (MQ=11)
                                                         tttttACGTAAATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGa                                                                                                                             <  5:300277/107‑1 (MQ=12)
                                                                gTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATCTTTCAGACCCTCCAAAAGCAAAAAACCGCCGCAG‑CGGGTTTTTACGGAAATCAGGTGAAACTGACCGAAAACCCGGGTTCTGt                                                                         >  2:1338043/1‑151 (MQ=14)
                                                                 tAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATCTTTCTGCCCCACCAAAACCAAAAACCCGCCGCAG‑CGCGTTTTTAAGTAAATCAGGTGAAACTGCCCCATAAGCCCGGTTCTGtg                                                                        >  2:590018/1‑150 (MQ=14)
                                                                   aaCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCGTTGAGCCCCGGCAAAAACAAAAACCCGCCTAAG‑GCGGTTTTTAAGTAAATCACGGGCAATTGCCCCATAAGCCGGGTGCTGtgtt                                                                      >  5:883321/1‑148 (MQ=14)
                                                                       aGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGCCAGTCATTCATGTTTGAGCCCCACCAAAACCAAAAACCCGCCGAAGCCGGGGTTTTA‑GTAAAACACGTCAAAATTACCCATAATCCGGGTTgtgtttgggat                                                                  >  6:302978/1‑144 (MQ=2)
                                                                       aGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGCAAGGCCTTCAAGTTTGTGCCCCAGCAAAAACAAAAAACCGCCGCAG‑CCGGTGTTTACCGTAATCATGGGCAACTGGCCGATCAGCCGGGTTgtgtgtgggtt                                                                  >  8:249394/1‑144 (MQ=2)
                                                                       aGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑ACGGGTATTAAGTAAATCACGTGAAAATTACCCATAAGCCGGGTTCAGTAGTGGa                                                                   >  7:54001/1‑150 (MQ=14)
                                                                       aGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGGCATTCATCTTTCGGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑GGGGTTTTTACGTAAATCAGGTGCAAATGCCCGATAATCCGGGGTGGGTCGTgggc                                                                  >  2:906416/1‑149 (MQ=11)
                                                                         gTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGGCAGTCATTCATATTTGTGCCCCGCCAAAAACAAAAAACCCCCGCAG‑CGGGGTTTTTCGTAAATAACGGGAAAATGACCGCTAAGCCGGGTTGTGtggtggcc                                                                  >  2:664408/1‑147 (MQ=14)
                                                                          tGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGCCAGGCAATAAAAATTAAGCCCCAACAAAAACAAAAAACCGCCGAAG‑ACCGTTTTTAAGTAAAACAAGAGAAAATTACCCATAAAACGGGttatttttgggaaatg                                                               >  4:836409/1‑137 (MQ=2)
                                                                            tatattatataTAATCCTCTTTTTTTTTTGTATAGGGTCTTGGTATGTCATTCATCTTTCTGCCCCTCCAAAAGGAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtc                                                              <  3:492187/140‑1 (MQ=14)
                                                                                 gtcctaTAAGCAGCTTTCTTTTGGGTAAAGCCGAGCTGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CTGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtcattca                                                         <  2:1005413/145‑1 (MQ=17)
                                                                                 gACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTGATTGATGTTTATGCACCTCCAAAAGCAAAAACACGACGAAG‑CGGGGTTTTACGTAAATCAGGTGAAACTGCCCGATAACACGGGTTCTGTCGTCGACAGtccttca                                                         >  8:122541/1‑150 (MQ=14)
                                                                                  aCCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAACCGCCGAAG‑CGGGTTTTTAAGTAAATAAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGGGGCCCGTGCTTCATc                                                       >  3:641832/1‑151 (MQ=14)
                                                                                   ccGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGAATAGCCGGGTTCTGTGGTGGACAGTCATTAATCt                                                      >  1:600764/1‑151 (MQ=255)
                                                                                    cGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAACCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGCCAGTCATTCATCt                                                      >  8:1054916/1‑150 (MQ=255)
                                                                                    cGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTa                                                     <  3:1175465/151‑1 (MQ=255)
                                                                                      aTAAGCCGCTTTCTTTTTTTTTTAGGTTCGTGGAAAGTCATTCATCTTTATTATAGTCTAAAAGCATTAACTCGCCGAAG‑CTGTTTTTTTCTTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAgg                                                   <  3:307565/151‑1 (MQ=12)
                                                                                       tAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCAGCCCCTCCAAAAGCAAAAACCCGCCGCAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGCCAGTCATTCATCTAGGc                                                  >  3:142278/1‑151 (MQ=255)
                                                                                       tAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGGCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGCCAGTGATTCATCTAGGc                                                  >  8:895222/1‑151 (MQ=255)
                                                                                        aaGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTAAATAATAATTCTGCCCCAACAAAAAAAAAAAAACCCCGAAG‑AGGGTTTTTACCTAAAAAACGTGAAAATGAACCATAAGCCCCGTTCTTTGGTGGAAAGtatataggaaaatcc                                                 >  4:1459022/1‑137 (MQ=2)
                                                                                           ccGCTTTCTTTTGGGTATAGCGTCGTGGACAGTAATTCATATTTATGCCCCTACAAAAGCAAAAAACCCCCGAAG‑CGGGTTTTTAAGTAAATAAGGTGAAACTGACCGATAAGCCGGGTTGTTTGGGGGACAGTCATTAATCTAGGCAAGc                                              >  4:693967/1‑151 (MQ=14)
                                                                                           ccGCTTTCTTTTGGGTATAGCGTCGTGGAAACTAATTAATATTTCTTACCCTAAAAAATCAAAAAACCCCCAAAG‑CGTGTTTTTAAATAAATAAGGTGAAAATTACCAATAAACCGGGTTCTGTAGTGGCAAGTCATTCATATATGCCAGc                                              >  3:1211123/1‑151 (MQ=12)
                                                                                            cGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGc                                              >  5:621602/1‑150 (MQ=255)
                                                                                             gCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCa                                             >  5:749194/1‑150 (MQ=255)
                                                                                                ttctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAACCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGCCCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCg                                         >  2:455212/1‑151 (MQ=255)
                                                                                                 tctttTGGGTATAGCGTCGTGGAAAAAAAATTAAATTTAAGGCCCTTCAAAAGCAAAAACCCCACGAAC‑AGCGTTTTTACATAAATAATCTGAAAATAACCGATAAACCGGGTACAGTCATTGAAACTAAATCATCTAGGCCatccaaacc                                        >  3:1226834/1‑143 (MQ=255)
                                                                                                    tttGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTAAGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTc                                      >  1:491695/1‑150 (MQ=255)
                                                                                                      tgggttttcggtagcggACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCAc                                    <  6:1040998/135‑1 (MQ=255)
                                                                                                      tGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTTCTCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCAc                                    <  4:908651/150‑1 (MQ=255)
                                                                                                       gggTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTg                                  >  8:602749/1‑151 (MQ=255)
                                                                                                        ggTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAACCGCCGAAG‑CGGGTTTTTACGTTAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGGCAGTCCTTCATCTAGGCCAGCAATCGCTCACTgg                                 >  2:919347/1‑151 (MQ=255)
                                                                                                          tataGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTAAAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCt                               >  7:500643/1‑151 (MQ=255)
                                                                                                           tcagatctgtGGACAGTCATTCATCTTTCTGCATCTACAAAAGTAAAAACCAGCAGATG‑CTTGTTTTTACTTAAATCAGGTGAAACTGACCTATAAGCGGTTTTGTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCt                               <  3:154163/142‑1 (MQ=18)
                                                                                                           atcgcttggtGGACAGTCATTCATCTCGCATCACCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTc                              <  2:9615/143‑1 (MQ=21)
                                                                                                            taGTGTCGTGGACAGTCATTCATTTTTTTGTAATTACAATTGTAAAAACGAGATGAAG‑AGTTTTTTTACGTAAATTAGGTTAAACTTAGCGATAAGGATGTTTGTGTCGTGGACAGTCATTCATCTAGGcc                                                 <  7:825194/131‑1 (MQ=9)
                                                                                                             aGCGTCGTGGACAGTGATTGATCTTTATGCCCCTCCAAAATCAAAAACCCGCCGAAG‑CGGGTTTTTAAGTAAATCAGGTGAAACTGCCCGATAAGCCGGGATCTGTCGTGGCCAGTCATTCATCTAGGCCAGCAATCGCGCACTGGCTCa                             >  1:1233102/1‑150 (MQ=21)
                                                                                                              gtttcgtTGACATTCATTCTTCTTTCTTTCCCTACAATAGGATAAACCGTCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAag                           <  1:71933/148‑1 (MQ=21)
                                                                                                               tgtcgtGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATaa                                                                                     <  6:1185526/91‑1 (MQ=14)
                                                                                                               tgtcgtGGACAGTCATTCATCTTTCTGACCCTCCAAAAGAAAAAACCCGCCGAAT‑AGGGTTTTTACGTAATACAGGTGAAACTGACCCATAAGCCGGTTTCTGTAGTGTAGAGTCATTAATCTAGGAAATCAAAAGTTCGCTGGCGCAagc                          >  2:1044666/2‑151 (MQ=2)
                                                                                                               ggtcgtGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagc                          <  5:1302709/150‑1 (MQ=255)
                                                                                                                gtcgtAGAAAGTAATTCTTCTTTATGGCCTTCAAAATTTAAATACACGACGATG‑TTGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagca                         <  3:429886/151‑1 (MQ=255)
                                                                                                                 tcttGGACATTCATTCAGCTTTCTCCCCCTCCAAAAGCTACTCGAGGGCCTAG‑GTGGTTTTTTCGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagca                         <  8:439874/147‑1 (MQ=25)
                                                                                                                   gtGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTAGTGGa                                                                   >  3:387555/1‑106 (MQ=255)
                                                                                                                   gtGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCCTCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGcc                      >  5:820625/1‑151 (MQ=255)
                                                                                                                   gtGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGcc                      >  6:621493/1‑151 (MQ=255)
                                                                                                                    tGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGTCCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCt                     >  7:1237639/1‑151 (MQ=255)
                                                                                                                    tGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCt                     >  1:532113/1‑151 (MQ=255)
                                                                                                                     ggACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCt                     >  6:1466739/1‑150 (MQ=255)
                                                                                                                     ggACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCt                     >  1:1117274/1‑150 (MQ=255)
                                                                                                                     ggACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCt                     >  5:71640/1‑150 (MQ=255)
                                                                                                                         aGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCg                >  2:268418/1‑151 (MQ=255)
                                                                                                                         aGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCg                >  5:1184791/1‑151 (MQ=255)
                                                                                                                            cattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGt             >  1:754935/1‑151 (MQ=255)
                                                                                                                            cattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGt             >  8:569179/1‑151 (MQ=255)
                                                                                                                            cattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGt             <  4:394216/151‑1 (MQ=255)
                                                                                                                             attcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGtt            >  7:1385135/1‑151 (MQ=255)
                                                                                                                             attcatCTATCAGCCCCTACAAAAGCAAATACGGGCCGACG‑CGTGTTATTACGTTAAGCCGGCGAACCTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGtt            <  6:903185/151‑1 (MQ=37)
                                                                                                                             atgcatCTTTCTGCACCTCCAACACGAAAACCCCTCCGAAG‑CTTGTTTTTAGTTAAATCAGGTGAAACTGAGCGATAAGCGGTCTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGtt            <  2:1016528/148‑1 (MQ=25)
                                                                                                                              ttcatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTGTCGTGGAAAGTGAATGGTCTAGGCCATCAATCGGTGCCGGGCGCAagcacaataacccgctgc           >  5:1315413/1‑137 (MQ=11)
                                                                                                                              ttcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTc           >  2:1230654/1‑151 (MQ=255)
                                                                                                                               tcatcaTTGTGCGCATCCCAACTCTAAAACCCGCCGCAG‑CGGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTc           <  2:749861/150‑1 (MQ=37)
                                                                                                                                tatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCa          <  4:779261/149‑1 (MQ=255)
                                                                                                                                gatCTTACTGGCACTCCAAAAGGAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCa          <  8:769829/149‑1 (MQ=255)
                                                                                                                                catCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTc           >  8:846696/1‑149 (MQ=255)
                                                                                                                                catCTTTCTGCACCTCCAAAAGCAAAAACCTTCCGAAG‑CTGGTTTTTACTTAAATCTGGTGAATCTGAGCGATATTCCGGTTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCa          <  1:100410/150‑1 (MQ=25)
                                                                                                                                aatCTTTCTGCCCCTCAAATAGTAAAAAGCCTACTATG‑TTTGTTTTTACGTATATCTGGTGATATTGAGTGATTATGGGGTTTGTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAg         <  3:229341/150‑1 (MQ=255)
                                                                                                                                  tCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGt        >  6:580541/1‑150 (MQ=255)
                                                                                                                                   cTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGGGTTCTTTAGTGTAAAGTGTGTGATCTAGTCAATCAAACGCTCCCTGCCTCAACCACACTACCCCGGGTCaagcg      >  2:778558/1‑147 (MQ=2)
                                                                                                                                    tttCTGCCCCTCCTAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACg     >  1:291122/1‑151 (MQ=255)
                                                                                                                                     ttCTGACCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTCGCTCAAGCAGCGTACCCGGGTTCAGTACgg    >  5:1164720/1‑151 (MQ=255)
                                                                                                                                      gcTGCCCGTCCAAAAGAAAAACCCGGCGGAAG‑CGGGTTTTTACTTAAAGCAGG‑GTAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCACTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGc  <  6:8969/150‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                                       
TCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGC  >  NC_000913/3270032‑3270317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑