Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,0600CT25.3% 630.5 / inf 487intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base C (166/164);  new base T (58/54);  total (256/231)
Fisher's exact test for biased strand distribution p-value = 8.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.87e-15

Only 100 of 491 total aligned reads displayed.

AGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTAC  >  NC_000913/4295910‑4296210
                                                                                                                                                      |                                                                                                                                                      
aGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAAc                                                                                                                                                        >  2:1618/1‑151 (MQ=31)
  gTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGc                                                                                                                                                      >  6:779138/1‑151 (MQ=31)
     ggATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTg                                                                                                                                                   >  2:26981/1‑151 (MQ=31)
      taTAATGCGTTTATGGCTTATGCGATTTGAATGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTg                                                                                                                                                   <  4:601450/149‑1 (MQ=11)
       aTAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAt                                                                                                                                                 >  6:255070/1‑151 (MQ=14)
       aTAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGAACTTTACACCGAATCCGACATCAACGCCTGAt                                                                                                                                                 >  4:228674/1‑151 (MQ=255)
        tAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGACAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCCTCCGACATCAACGCCTGATg                                                                                                                                                >  2:545164/1‑151 (MQ=14)
         aaGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGc                                                                                                                                               >  4:1456507/1‑151 (MQ=14)
         aaGGCGTTTATGCCGCAGCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATg                                                                                                                                                <  8:1273233/150‑1 (MQ=14)
             gttttATGCCGGATCAGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACg                                                                                                                                           <  3:150077/149‑1 (MQ=14)
              gTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGc                                                                                                                                          >  8:671207/1‑151 (MQ=14)
              gTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGc                                                                                                                                          >  4:817184/1‑151 (MQ=14)
              gTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGc                                                                                                                                          >  1:1009674/1‑151 (MQ=14)
                 tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTg                                                                                                                                       >  2:741879/1‑151 (MQ=17)
                  aTGCCGCATCCGACATCAAAGCCTGATGCGACACATAACAAGACATAACAGGACTAAAACAGACAACGCAATAGACTAATTTAGCGTGATTTTCTATTACTTAAAAAAAGTTTACGCCGCATCCGACATCAAGGCCTGATGCGCCGGTTgg                                                                                                                                      >  3:520709/1‑150 (MQ=255)
                    gCCGCTTCCGACTTCAAGGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGGCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGcc                                                                                                                                     <  1:1104940/150‑1 (MQ=255)
                    gCCGCATCCGACATCAACGCCTGATGCGACGTGTACCGCGTCTTTTCAGGCCTACGCCAGACACCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGcc                                                                                                                                     <  6:701070/150‑1 (MQ=11)
                    gCCGCATCCGACATCAACGCATGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCTCAATAGACTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGcc                                                                                                                                     <  1:449555/150‑1 (MQ=255)
                    cccGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGcc                                                                                                                                     <  1:698438/149‑1 (MQ=18)
                     ccGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCcgc                                                                                                                                   >  6:1353686/1‑151 (MQ=21)
                         aTCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATATAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAAGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCt                                                                                                                               >  6:476991/1‑151 (MQ=18)
                           ccGACATCAACGCCTGATGCGACTCTTTACTCGTCTTATCAGGCCTACGCCAGACAGCGAAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTa                                                                                                                             <  7:395220/151‑1 (MQ=255)
                           ccGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCGAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCttt                                                                                                                             >  5:1480984/1‑150 (MQ=25)
                            cGACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                            <  6:1259195/151‑1 (MQ=25)
                             gACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAAGCCGCATCCGACATCAACGCCTGATGCTACGCTTGCCGCGTCTTAt                                                                                                                            >  3:1210508/1‑150 (MQ=18)
                             cacctCAACCCCCGAGGCGACGCGCCACCCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATc                                                                                                                           <  6:948429/147‑1 (MQ=11)
                              agatgaaCTCCTGATGCTAAGATTTAGGCGTCTTATCAGGCTTACGCGAGTCTGATAAATATCCTGATTTAGCTTGATTTTGTATGTAGTATATGGCTTTTACTCCGCATAAGACATGAACGCCTGATGCGACGCTTGCCGCGTCTTATCa                                                                                                                          <  3:1088185/146‑1 (MQ=2)
                              aCATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTTATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCa                                                                                                                          <  5:1183576/151‑1 (MQ=21)
                               cATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCATGATGCGACGCTTGCCTCGTCTTATc                                                                                                                           >  8:10060/1‑149 (MQ=25)
                                aTCAACGCCTGAGGCGAGGCTACCCGCGTCTTTCCACGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg                                                                                                                        <  5:626830/151‑1 (MQ=11)
                                 tCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGACAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAAAGCCTGATGCGACGCTTGCCGCGTCTTATAAGGc                                                                                                                       >  1:218150/1‑151 (MQ=255)
                                   aaCTCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGAGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCt                                                                                                                     <  5:774648/151‑1 (MQ=25)
                                   aaCGCCTGAGGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCt                                                                                                                     <  2:1278548/151‑1 (MQ=21)
                                    aCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTa                                                                                                                    <  7:694272/151‑1 (MQ=35)
                                     cGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTat                                                                                                                   >  1:773900/1‑150 (MQ=18)
                                      gacTGATGCTACGCTTTCCGCTTCTTATGAGTCCTACTCCTCACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatc                                                                                                                  <  5:1283691/149‑3 (MQ=11)
                                       ccTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatct                                                                                                                 >  8:288735/1‑148 (MQ=17)
                                          gATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGcc                                                                                                                >  7:490201/1‑149 (MQ=35)
                                          gATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAg                                                                                                              <  5:317099/151‑1 (MQ=35)
                                           aTGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAAGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAGGGCAgc                                                                                                             >  8:1125279/1‑150 (MQ=16)
                                             gCGACGCTTCACGCGTCTTATCAGGCCTACGCCAGACAGAGCAATAGCCTGATTTAGCTTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACa                                                                                                           <  7:83728/151‑1 (MQ=255)
                                             gCGACGCTTAACGCGTCTTATCAGGCCTACGCCATACAGCGCAATAGCCTGATTTAGCTTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACa                                                                                                           <  3:756513/151‑1 (MQ=25)
                                                 cGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAAAGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTaacaaacacagagc                                                                                                       >  3:989824/1‑138 (MQ=255)
                                                    ttAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCATCATTTAGCGTGATATTGTAGGTCGGAAAAGGAGTTTAACACGCATCAGACAACAAAGCCAGATGCGACGCTTCCATCGTCTTATCAGGCCTACggtacccagagcta                                                                                                     >  1:88971/1‑137 (MQ=2)
                                                      aaCTCGTCTTATCAGGGCTACGCCAGTCAGCGAAATAGCCTGATTTAGGGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCTTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATa                                                                                                   <  2:451067/150‑1 (MQ=11)
                                                         gcgTCTTTTCAGGCGTAAGCCATACAGATCAATAGGCTGATTTTCTGTGATTTTGTTTGGCTGATAAGGGTTTTACGCCGCTTAAGTGATCAAGGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCt                                                                                               <  3:827673/151‑1 (MQ=2)
                                                          cgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTAAGAAAGACATAGCAATAgaatt                                                                                              >  4:521518/1‑147 (MQ=11)
                                                               tATCAGGCCTACGCCAGTCAGCGCAATAGCCTGATTTAGCGTGATTTTGTATGTCGGATAAGGCGTTTACGCCGCATAAGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAtt                                                                                           <  3:632898/149‑1 (MQ=255)
                                                               tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTCGCCTGATTTTGTAGGGCGGATACGGCTTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAttt                                                                                          <  6:659157/150‑1 (MQ=255)
                                                               tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATGAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAt                                                                                            <  2:1186543/148‑1 (MQ=25)
                                                               tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAAAGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAAAAGCCTGAtgt                                                                                          >  2:1049797/1‑148 (MQ=255)
                                                               tATCACTCCTACGCAAGACTGCGCAATAGGCTGATTTAGCGTTATTTTTTTTTTAGTTTATGGCTTTTACTCCGCTTAATATTTCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAt                                                                                            <  7:930765/148‑1 (MQ=2)
                                                                aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGACGCGTCTTATCAAGCCTACGCCAGACAGCGCAATAGGCTGAttt                                                                                          >  6:1191063/1‑149 (MQ=9)
                                                                       cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTCATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAtt                                                                                 >  2:68610/1‑151 (MQ=25)
                                                                           gccagccaTCGCTATAGGCTGATTTAGCTTGATATTTTATTTCTTATCAGGGTTATACCCCGCTTCATTTATGAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGt                                                                             <  2:936796/151‑1 (MQ=2)
                                                                           gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCTCAAAAGAATCAATTAGAGTGAttttat                                                                             >  4:1360911/1‑149 (MQ=11)
                                                                             cAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGGCATCAAAGACTGCAGCTCGGCTTTCCGCGACTTATAACGCCTACGCCAGCCCGCGCAATAGCCTGAAATAGAGTGATTTTGt                                                                             >  5:456410/1‑149 (MQ=11)
                                                                              cgccAGCGCACCAGCCGGACTTAGCCTGATTGTGCAGGTCGGATAATTCTTTTATGCCTCATCCGACATCAACGCATGAAGCGACGATTGCCGCTTATTATCAGGCGGACGCGATACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAgg                                                                          <  8:1460387/148‑1 (MQ=2)
                                                                               caCAGCGCAATACCCTGATTTAGCGTGATTTGGTAGGTCGCATACGGCGGTTACGCCGGATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGt                                                                         <  5:421914/150‑1 (MQ=11)
                                                                                 cAGCGCACTACCCTGATGAAGCGTGATTATGTCCGCCGGATAATTCTTTTTCCCGCCCTCCGACATCTACGCCTTATGCTACGCTTACGGCCTCTCATCAGTGCGAGGTTACAATGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCg                                                                       <  5:2873/151‑1 (MQ=2)
                                                                                     gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGAATAAGGAATATAGGCCTCAACCGAAATAAACGACTGANTAGACTATATCAGCGTCTTATCAGTAATAATAAAAACAAAGNAATAGCCTGATTTAGCGTGCTTTTGTAGGTCgggtt                                                                   >  1:2869/1‑148 (MQ=2)
                                                                                        aTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCAGCGCTATAGCCTGAGTTAGCGTGATTTTGTAGGACGGATAAgg                                                                >  8:212764/1‑151 (MQ=255)
                                                                                               gATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAt                                                         >  2:328755/1‑151 (MQ=35)
                                                                                                aTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAACAAAGACAGAGCAATAGCATCATTTAACGTGATTTTGTAGGAAGGATAAGGCCTTTATg                                                        >  4:114543/1‑151 (MQ=11)
                                                                                                  ttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCGTACCACAGACATAGCAATATAATGATTTATCGTGAATATGTAGGTCTGATAAGGCGTTTaagac                                                      >  7:73518/1‑147 (MQ=11)
                                                                                                  ttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGcc                                                      >  8:50667/1‑151 (MQ=35)
                                                                                                   tAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCg                                                     >  6:572640/1‑151 (MQ=35)
                                                                                                   tAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCAAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAAGca                                                      >  4:70655/1‑149 (MQ=255)
                                                                                                       gTGATTTAGTCGCGCGGATCCGGCTTTTACCCCGCATAAGAGATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATc                                                 <  5:517625/151‑1 (MQ=11)
                                                                                                          aTTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCg                                               >  4:62746/1‑150 (MQ=35)
                                                                                                            tttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTCCCGCGTCTTATCAGGCCTAACAAAAACAGCGCAAAATACTGATTAAAATTCATAAAGTAGCTCACAAAAGGCGTTTATGCAGAATcacac                                             >  1:1343290/1‑146 (MQ=2)
                                                                                                             ttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTTAATTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCAGCATCCGACAt                                           >  8:886025/1‑151 (MQ=255)
                                                                                                              tgtagcgcgtctccggCGTCTACCCCGCACAATACATGAAGGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACAt                                           <  5:1089827/136‑1 (MQ=11)
                                                                                                               gTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCAGCATCAGACATc                                          >  1:1185984/1‑150 (MQ=25)
                                                                                                               ctAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGATTGCCGCGTCTTATCAGGCCTACGCAACACAGCGCAATAGCCTGAATTAGCGTCATATAGAAGCACCGAAAAGGCGATAATGCAGCATCAGACATc                                          >  7:1444691/2‑150 (MQ=11)
                                                                                                                tAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAGGCAAGACAGCGCAATATGGTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCa                                         >  6:852060/1‑150 (MQ=255)
                                                                                                                 aGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCAAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAAc                                       >  5:234793/1‑151 (MQ=25)
                                                                                                                      ggATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTg                                  >  2:819432/1‑151 (MQ=35)
                                                                                                                       gATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTg                                  >  2:616705/1‑150 (MQ=35)
                                                                                                                          aaGGCGTTTTAGGCGCTTCCGATATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATg                               <  3:1308037/150‑1 (MQ=11)
                                                                                                                                 ttACGCCGCATCCGACATCAACGCCTGATGCGACGCTTCCCGAGTCATAACAGGACTAAGCCAGACAGCGCAATAGACTAATTTACCGTGATATTCTAGGTCGTATAAAGAGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCtt                       >  4:931104/1‑151 (MQ=11)
                                                                                                                                     gccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGAATCCGACATCAAAGCCTGATGCGACGCTTAAcg                   >  4:346438/1‑151 (MQ=18)
                                                                                                                                      cctcagccGACTTCACCGCCGGCCGCGACGGTTCCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAAcgc                  <  8:1182763/145‑1 (MQ=11)
                                                                                                                                      ccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAAcgc                  >  2:1405206/1‑151 (MQ=33)
                                                                                                                                       cgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAAcgcg                 >  5:847421/1‑151 (MQ=33)
                                                                                                                                       cgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAAcgcg                 >  6:408264/1‑151 (MQ=33)
                                                                                                                                        gcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGt                >  6:1048508/1‑151 (MQ=33)
                                                                                                                                        gcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAGGCCGCATCCGACATCAACGCCTGATGCGACGCTTAAcgcgg                >  5:1039183/1‑150 (MQ=18)
                                                                                                                                        gcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAAGCCGCAGACGACATCAACGCCTGATGCGACGCTTAACGCGt                >  8:595453/1‑151 (MQ=16)
                                                                                                                                         cATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTc               >  8:1076063/1‑151 (MQ=33)
                                                                                                                                          aTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCt              >  7:1216975/1‑151 (MQ=33)
                                                                                                                                          aTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCt              >  2:845286/1‑151 (MQ=33)
                                                                                                                                            ccGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCtt             >  6:199779/1‑150 (MQ=33)
                                                                                                                                             cGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAAGCCGCATCCGACATCAAAGCCTGATGCGACGCTTAACGCGTCTTAt           >  7:1107099/1‑151 (MQ=18)
                                                                                                                                             cGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCAAGACAGCGCAATAGCATGATTTAGCTTGATTTTCTAGTTCGGATAATTCGTTTATGCAGAATCAGACAACAACGCCTGATGCGACGATTAACTCGTCTTAt           >  3:318824/1‑151 (MQ=11)
                                                                                                                                             cGACAGCAACGCCTGATGCGACGCTTGCGGCGGCTTAGCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTAt           <  6:339556/151‑1 (MQ=255)
                                                                                                                                               acctCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCGACGCCAGACAGCGCAATAGCCTGATTTCGCGTGATTTTGTAGGTCGGATACGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCa         <  6:1112498/148‑1 (MQ=255)
                                                                                                                                               aCATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATc          >  4:29554/1‑150 (MQ=33)
                                                                                                                                                 aTCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAg        <  3:42146/150‑1 (MQ=33)
                                                                                                                                                   cAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGcc     >  4:416686/1‑151 (MQ=32)
                                                                                                                                                      |                                                                                                                                                      
AGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTAC  >  NC_000913/4295910‑4296210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: