Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,187,667 T→A F773Y (TTC→TAC)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,187,6670TA93.8% 1228.0 / 45.8 363F773Y (TTC→TAC) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base T (13/9);  new base A (163/172);  total (181/182)
Fisher's exact test for biased strand distribution p-value = 3.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 365 total aligned reads displayed.

TGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCATGAT  >  NC_000913/4187520‑4187814
                                                                                                                                                   |                                                                                                                                                   
  atgGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACAtct                                                                                                                                                >  6:1350550/1‑151 (MQ=255)
    ggCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTcc                                                                                                                                              <  7:124253/151‑1 (MQ=255)
      ccGACACCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCCGCTTGCTGGTATGCGTGGTCTGAAGGCGAAGCCGGATGGCTCCATCCTCGAAACTCCAATCACCGCGAACTTCCGTGAAGGACTCAACGTACTCCAGTACTACATCTccac                                                                                                                                            >  4:894337/1‑151 (MQ=255)
          cTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTTGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTccacccac                                                                                                                                        >  5:458381/1‑151 (MQ=255)
            ccGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTccacccac                                                                                                                                        <  4:460966/149‑1 (MQ=255)
             cGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGt                                                                                                                                     <  1:115026/151‑1 (MQ=255)
              ggTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTg                                                                                                                                    >  5:987336/1‑151 (MQ=255)
                 gcgcGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTc                                                                                                                                 <  5:119511/151‑1 (MQ=255)
                  cgcgTGGGTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGACGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCg                                                                                                                                <  7:85607/151‑1 (MQ=255)
                   gcgTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGTGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGt                                                                                                                               <  8:1150188/151‑1 (MQ=255)
                       ggTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTaaa                                                                                                                            <  8:205177/150‑1 (MQ=255)
                       ggTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAg                                                                                                                           <  2:1405105/151‑1 (MQ=255)
                          tCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGt                                                                                                                         <  2:1472116/150‑1 (MQ=255)
                          tCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGt                                                                                                                         <  2:334216/150‑1 (MQ=255)
                          tCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTc                                                                                                                        >  4:682665/1‑151 (MQ=255)
                          tCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGCCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTc                                                                                                                        >  7:1457582/1‑151 (MQ=255)
                             gCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTgg                                                                                                                     <  3:1193000/151‑1 (MQ=255)
                              cGGCACACATTCGTCAGCTTGCTGGTATGCGTGGTCTGATAGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGc                                                                                                                    >  4:838396/1‑151 (MQ=255)
                                 cacaGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCgg                                                                                                                  >  2:459191/1‑150 (MQ=255)
                                  acaGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGAt                                                                                                                <  1:1067007/151‑1 (MQ=255)
                                    aGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATAc                                                                                                              >  1:705609/1‑151 (MQ=255)
                                    aGATTCGTCAGCGTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGGCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATAc                                                                                                              <  1:1475111/151‑1 (MQ=255)
                                       ttCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACAGc                                                                                                           >  2:846553/1‑151 (MQ=255)
                                          gTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCTTCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACt                                                                                                        >  3:209548/1‑151 (MQ=255)
                                           tCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTg                                                                                                       >  2:992845/1‑151 (MQ=255)
                                            cAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTg                                                                                                       >  4:448088/1‑150 (MQ=255)
                                              ggttGCTGGTTTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGaa                                                                                                     <  1:984044/148‑1 (MQ=255)
                                              gCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGaaa                                                                                                    <  8:454247/151‑1 (MQ=255)
                                              gCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGaaa                                                                                                    <  3:765150/151‑1 (MQ=255)
                                              gCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACACACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGaa                                                                                                     >  7:910100/1‑150 (MQ=255)
                                                ttGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAAc                                                                                                  >  8:719618/1‑151 (MQ=255)
                                                ttGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAAc                                                                                                  >  5:746341/1‑151 (MQ=255)
                                                 tGCTGGTATGCTTGGTCTGATCGCGAAGCCGGATGGCTACATCATATAAACGCCAATCACCGCGAACTTCAGTGAAGGTCTGAACGTACTCGAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAAc                                                                                                  <  2:736596/150‑1 (MQ=255)
                                                 tGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGaaaa                                                                                                   <  3:13925/149‑1 (MQ=255)
                                                  gCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTg                                                                                                <  6:183186/151‑1 (MQ=255)
                                                   cTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGc                                                                                               >  3:700367/1‑151 (MQ=255)
                                                    tGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCg                                                                                              >  8:1224532/1‑151 (MQ=255)
                                                     gttATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGc                                                                                               <  6:1291389/147‑1 (MQ=255)
                                                        aTGCGTGGGCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACt                                                                                          <  1:784253/151‑1 (MQ=255)
                                                          gCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTcc                                                                                        >  5:1420329/1‑151 (MQ=255)
                                                           cGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCg                                                                                       <  5:366804/151‑1 (MQ=255)
                                                              ggTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGtt                                                                                    >  5:1110140/1‑151 (MQ=255)
                                                              ggTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGtt                                                                                    >  7:1312706/1‑151 (MQ=255)
                                                                tCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTAc                                                                                  >  2:679852/1‑151 (MQ=255)
                                                                tCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTAc                                                                                  >  5:1006983/1‑151 (MQ=255)
                                                                gcTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTAc                                                                                  <  4:550695/150‑1 (MQ=255)
                                                                  tGATGTCGAAGCCGCATGGCTCCATCATCGAAACGCCAATCCCCGAGAACATCCGTGAAGGTCTGAACGTACTCAAGAACTACATCTCCACCCACGGAGCTCCTAAAGGACTGGAGGATACCGCACTGAAAACTGCGAACTACGGTTACCt                                                                                >  4:326172/1‑151 (MQ=255)
                                                                  tGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCCCCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCCCCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCt                                                                                >  2:713911/1‑151 (MQ=255)
                                                                   gATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTAcc                                                                                 <  2:762781/149‑1 (MQ=255)
                                                                   gATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTAc                                                                                  <  1:285636/148‑1 (MQ=255)
                                                                   gATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTg                                                                               <  8:142865/151‑1 (MQ=255)
                                                                       gCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACtc                                                                           >  3:1324766/1‑151 (MQ=255)
                                                                         naaGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACtcgt                                                                         >  1:420541/2‑151 (MQ=255)
                                                                          aaGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACtcgtc                                                                        >  5:690581/1‑151 (MQ=255)
                                                                          aaGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACtcgtc                                                                        <  4:1463545/151‑1 (MQ=255)
                                                                          aaGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACtcgtc                                                                        >  3:349012/1‑151 (MQ=255)
                                                                          aaGCCGGACGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGGCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACtcgtc                                                                        <  7:726333/151‑1 (MQ=255)
                                                                                gATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTgg                                                                  <  6:33571/151‑1 (MQ=255)
                                                                                   ggCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGtt                                                                <  7:372792/150‑1 (MQ=255)
                                                                                      gccATCATCGAACCGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACg                                                            <  2:1255224/150‑1 (MQ=255)
                                                                                       ccATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGAc                                                             <  6:951060/149‑1 (MQ=255)
                                                                                       ccATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCAGGTTACCTGACTCGTCGTCTGGTTGAc                                                             <  7:597134/149‑1 (MQ=255)
                                                                                         atgatcGAAACCCCAATCTCCGGGAACTTCCGTGAAGGTCTGAACTTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTATGGCGGATACCGCACTGAAAACTGAGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTgg                                                         <  2:1143807/148‑1 (MQ=255)
                                                                                           catcGAAACGCCAATCACCGCGAACTTCCGGGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGgc                                                        <  5:174877/150‑1 (MQ=255)
                                                                                              cGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAg                                                    <  6:706524/151‑1 (MQ=255)
                                                                                               gAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAgg                                                   >  8:883443/1‑151 (MQ=255)
                                                                                                aaaCGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAgg                                                   >  7:958772/1‑150 (MQ=255)
                                                                                                  aCGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCAGCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGAcc                                                <  3:850434/151‑1 (MQ=255)
                                                                                                       aaTCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAAc                                                                                           >  3:435962/1‑103 (MQ=255)
                                                                                                       aaTCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCtggtg                                           >  6:1433957/1‑151 (MQ=255)
                                                                                                        aTCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAAc                                                                                                  >  8:40009/1‑95 (MQ=255)
                                                                                                        aTCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCtggtgg                                          >  8:528851/1‑151 (MQ=255)
                                                                                                          cACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGtt                                        >  8:1276277/1‑151 (MQ=255)
                                                                                                          cACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGtt                                        <  7:114170/151‑1 (MQ=255)
                                                                                                               cgAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCg                                    >  6:1101477/1‑150 (MQ=255)
                                                                                                                 aaCTTCCGTGAAGCTCTGAACGTACTCCAGTACTTCATCTCCACACACGGTGCTCGTAAAGCTCTCCCGGATCCCGCACTGAAAACTGCGAACTCCGCTTACCTGACTCGTCGGCTGGTTGACGTGGCGCAGCACCTGGTGGTTACCCaac                                 >  7:26392/1‑150 (MQ=255)
                                                                                                                     tCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACg                              <  3:577451/150‑1 (MQ=255)
                                                                                                                     tCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACg                              >  2:906273/1‑150 (MQ=255)
                                                                                                                      ccGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGAt                            <  6:997080/151‑1 (MQ=255)
                                                                                                                      ccGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGAt                            >  3:826454/1‑151 (MQ=255)
                                                                                                                       cGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGAtt                           <  2:209543/151‑1 (MQ=255)
                                                                                                                        gTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGAttt                          >  4:1390848/1‑150 (MQ=255)
                                                                                                                         tGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATtgt                         >  6:886179/1‑151 (MQ=255)
                                                                                                                          gAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATtgtg                        >  4:399173/1‑151 (MQ=255)
                                                                                                                            aGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGt                      >  6:598188/1‑151 (MQ=255)
                                                                                                                                  gAACGTACTGCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATAGCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGGCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTTGTAGCCa                 <  4:1026189/150‑1 (MQ=255)
                                                                                                                                  gAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCAt                >  4:1255100/1‑151 (MQ=255)
                                                                                                                                     cGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGa              >  1:1419730/1‑150 (MQ=255)
                                                                                                                                     cGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGaa             <  6:55934/151‑1 (MQ=255)
                                                                                                                                     cGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGa              >  5:42118/1‑150 (MQ=255)
                                                                                                                                        aCTCCAGTACTACATCTCCACCCACGCTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAgg           <  1:223202/150‑1 (MQ=255)
                                                                                                                                         cTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGt          >  1:1373189/1‑150 (MQ=255)
                                                                                                                                           ccAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTAt        >  4:156994/1‑150 (MQ=255)
                                                                                                                                           ccAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATc       <  2:568643/151‑1 (MQ=255)
                                                                                                                                           ccAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATc       <  7:636656/151‑1 (MQ=255)
                                                                                                                                             aGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCat     >  3:1113659/1‑151 (MQ=255)
                                                                                                                                              gTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCatg    >  6:833075/1‑151 (MQ=255)
                                                                                                                                               tactacATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCatg    >  1:1232675/1‑150 (MQ=255)
                                                                                                                                                aCTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCatgat  <  8:665186/151‑1 (MQ=255)
                                                                                                                                                aCTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGGTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCatga   <  8:109847/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCATGAT  >  NC_000913/4187520‑4187814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: