Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 962,358 G→C V122L (GTT→CTT)  rpsA → 30S ribosomal subunit protein S1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913962,3580GC95.4% 1224.7 / 30.6 326V122L (GTT→CTT) rpsA30S ribosomal subunit protein S1
Reads supporting (aligned to +/- strand):  ref base G (5/10);  new base C (164/145);  total (170/156)
Fisher's exact test for biased strand distribution p-value = 1.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 326 total aligned reads displayed.

GGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTGTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTCGTC  >  NC_000913/962210‑962505
                                                                                                                                                    |                                                                                                                                                   
ggACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCtt                                                                                                                                                   <  7:118948/151‑1 (MQ=255)
 gACGCAGTAGAAGACGGCTTCGGTGAATCTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTGTTg                                                                                                                                                  <  2:346184/151‑1 (MQ=255)
  aCGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTg                                                                                                                                                  >  3:1188475/1‑150 (MQ=255)
  aCGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGa                                                                                                                                                 <  8:697890/151‑1 (MQ=255)
  aCGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGa                                                                                                                                                 <  8:200402/151‑1 (MQ=255)
      aGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTg                                                                                                                                             <  7:1096328/151‑1 (MQ=255)
      aGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTg                                                                                                                                             <  2:737175/151‑1 (MQ=255)
         agaagaCGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAAc                                                                                                                                          <  6:320495/151‑1 (MQ=255)
         agaagaCGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGGGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAAc                                                                                                                                          <  4:731810/151‑1 (MQ=255)
          gaagaCGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACg                                                                                                                                         >  5:490171/1‑151 (MQ=255)
           aagaCGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACgg                                                                                                                                        >  4:52180/1‑151 (MQ=255)
            agaCGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGt                                                                                                                                       <  1:1037416/151‑1 (MQ=255)
               cGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTAtt                                                                                                                                    <  4:640169/151‑1 (MQ=255)
               cGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTAtt                                                                                                                                    <  1:4559/151‑1 (MQ=255)
                ggCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTc                                                                                                                                   >  2:1471589/1‑151 (MQ=255)
                  cTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGt                                                                                                                                 >  4:1126235/1‑151 (MQ=255)
                    tCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTg                                                                                                                                <  5:717922/150‑1 (MQ=255)
                      ggTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGt                                                                                                                             >  3:1035134/1‑151 (MQ=255)
                       gTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACCTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTCTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTATTGAGGAGAACGGTATTCGTGCGtt                                                                                                                            >  8:55801/1‑151 (MQ=255)
                            aCTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGc                                                                                                                       <  2:677084/151‑1 (MQ=255)
                                tgctgTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAgg                                                                                                                   >  1:1455070/1‑151 (MQ=255)
                                 gctgTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTGTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGt                                                                                                                  <  8:487810/151‑1 (MQ=255)
                                  ctgTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGtt                                                                                                                 >  2:585569/1‑151 (MQ=255)
                                  ctgTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGAGGCTTCACTCTTGAGCTGAACGGGATTCGTGCGTTCCTGCCAGGtt                                                                                                                 >  5:302537/1‑151 (MQ=255)
                                   tgTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGttg                                                                                                                >  1:1148715/1‑150 (MQ=255)
                                   tgTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTtc                                                                                                                >  5:747642/1‑151 (MQ=255)
                                    gTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTtct                                                                                                               >  2:1279134/1‑151 (MQ=255)
                                    gTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTtct                                                                                                               >  1:353685/1‑151 (MQ=255)
                                     tCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTtctc                                                                                                              >  4:331852/1‑151 (MQ=255)
                                           gagaAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAg                                                                                                        <  1:562233/151‑1 (MQ=255)
                                            agaAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGa                                                                                                       <  2:1101110/151‑1 (MQ=255)
                                              aaaGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACg                                                                                                     <  6:17375/151‑1 (MQ=255)
                                              aaaGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACg                                                                                                     >  5:963797/1‑151 (MQ=255)
                                               aaGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACGCTTGAGCTGAACGGTATTGGTGCGTTCCTGCCAGGTTCTCTGGTAGACGt                                                                                                    >  3:15735/1‑151 (MQ=255)
                                                  cTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTGTTGAGCTGCACGGTATTCGTGCGTTCCTGCCCGGTACTCTGGTAGACGTTCg                                                                                                 >  4:1376123/1‑151 (MQ=255)
                                                   tAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGt                                                                                                >  6:1479617/1‑151 (MQ=255)
                                                    aaaCGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCg                                                                                                 <  6:1327775/149‑1 (MQ=255)
                                                    aaaCGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTc                                                                                               >  8:1429240/1‑151 (MQ=255)
                                                       agTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATAAACGGCAAAGTAAAGCGCGGCTTCACTCTTGAGCTGAACGGTATTCGTCAGTTCCTCCAAGGATCTCTGGTAGACGTTCGTACgg                                                                                            >  7:130882/2‑151 (MQ=255)
                                                          cACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTGTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTACGGTGc                                                                                         <  7:98429/151‑1 (MQ=255)
                                                           aCGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCg                                                                                        <  3:799338/151‑1 (MQ=255)
                                                           aCGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCg                                                                                        <  8:43820/151‑1 (MQ=255)
                                                             gAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTg                                                                                      <  1:1112795/151‑1 (MQ=255)
                                                             gAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGATCGTCCGGTGCGTg                                                                                      >  5:1283908/1‑151 (MQ=255)
                                                                   tGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACActc                                                                                >  6:1049980/1‑151 (MQ=255)
                                                                        cACGCTGGAAAAAGCTTACGAAGATGCTGACACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTGCACTCTTGAGCTGAACGGTATTCGTGCGTTCTTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCAc                                                                           <  3:140831/151‑1 (MQ=255)
                                                                         aCGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCAcc                                                                          <  8:638597/151‑1 (MQ=255)
                                                                         aCGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCAcc                                                                          <  8:1332606/151‑1 (MQ=255)
                                                                          cGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCTTGACACTCTGccccg                                                                         >  8:906787/1‑150 (MQ=255)
                                                                          cGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCt                                                                         <  8:368054/151‑1 (MQ=255)
                                                                              ggAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGaa                                                                     <  2:1054053/151‑1 (MQ=255)
                                                                                aaaaaGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTGTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAgg                                                                   <  6:539169/151‑1 (MQ=255)
                                                                                aaaaaGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAgg                                                                   <  5:1207846/151‑1 (MQ=255)
                                                                                  aaaGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCa                                                                 <  7:551610/151‑1 (MQ=255)
                                                                                    aGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCaa                                                                >  3:667167/1‑150 (MQ=255)
                                                                                     gCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAag                                                              <  1:164635/151‑1 (MQ=255)
                                                                                        tACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAaga                                                             <  3:533401/149‑1 (MQ=255)
                                                                                        tACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTAAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCAAGGTTATCTGGTAGAAGTTCGTCCGGTGCGTGACACTCTGCAACTGGAAGGAAAAGAGc                                                           >  5:619330/1‑151 (MQ=255)
                                                                                         aCGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCt                                                          >  3:814359/1‑151 (MQ=255)
                                                                                          cGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCtt                                                         <  3:635734/151‑1 (MQ=255)
                                                                                          cGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGACTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCtt                                                         <  3:490499/151‑1 (MQ=255)
                                                                                           gAAGATGCTGAAACTGTTTCCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTg                                                        <  7:101209/151‑1 (MQ=255)
                                                                                             aGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGaa                                                      <  4:21841/151‑1 (MQ=255)
                                                                                               aTGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAAtt                                                    >  5:84986/1‑151 (MQ=255)
                                                                                                  cTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTa                                                  >  3:203487/1‑150 (MQ=255)
                                                                                                   tGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGCCACTCTGCACCTGGAAGCCAAAGAGCTTGAATTTaa                                                 >  3:898847/1‑150 (MQ=255)
                                                                                                      aaCTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTa                                             <  7:86719/151‑1 (MQ=255)
                                                                                                      aaCTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTa                                             <  8:1197355/151‑1 (MQ=255)
                                                                                                       aCTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTa                                             >  2:86682/1‑150 (MQ=255)
                                                                                                       aCTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTa                                             >  8:307638/1‑150 (MQ=255)
                                                                                                         tGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATc                                          >  5:1307021/1‑151 (MQ=255)
                                                                                                         tGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATc                                          >  5:1442466/1‑151 (MQ=255)
                                                                                                         tGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAAGTTAAAGTAAt                                           >  7:809636/1‑150 (MQ=255)
                                                                                                          gTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCa                                         <  7:156809/151‑1 (MQ=255)
                                                                                                          gTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTGCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATc                                          <  1:66111/150‑1 (MQ=255)
                                                                                                            tACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCaa                                        <  8:1086636/150‑1 (MQ=255)
                                                                                                            tACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCaa                                        <  7:1447151/150‑1 (MQ=255)
                                                                                                             aCCTGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGc                                      >  1:218623/1‑151 (MQ=255)
                                                                                                             aCCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGc                                      >  8:1269967/1‑151 (MQ=255)
                                                                                                               cGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTGTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTg                                    <  1:1183516/151‑1 (MQ=255)
                                                                                                                     tATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATc                                >  8:150494/1‑149 (MQ=255)
                                                                                                                     tATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATc                                >  4:563136/1‑149 (MQ=255)
                                                                                                                     tATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATc                                >  6:808041/1‑149 (MQ=255)
                                                                                                                      aTCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGa                             <  2:52278/151‑1 (MQ=255)
                                                                                                                        cAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAg                           <  1:932295/151‑1 (MQ=255)
                                                                                                                         aaCGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAgc                          >  7:276532/1‑151 (MQ=255)
                                                                                                                           cGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAgcgc                        >  8:825960/1‑151 (MQ=255)
                                                                                                                               aaaGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGcaaca                    <  4:1441061/151‑1 (MQ=255)
                                                                                                                                     aaGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACgtt               <  6:1158688/150‑1 (MQ=255)
                                                                                                                                       gggCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACgttgtt            <  3:577795/151‑1 (MQ=255)
                                                                                                                                        ggcggcTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGGGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACgttgttg           <  4:1061615/151‑1 (MQ=255)
                                                                                                                                        ggcggcTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACgttgttg           >  1:832471/1‑151 (MQ=255)
                                                                                                                                         gcggcTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACgttgttgt          <  4:1252835/151‑1 (MQ=255)
                                                                                                                                          cggcTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACgttgttgtt         <  5:262961/151‑1 (MQ=255)
                                                                                                                                           ggcTTCACTGTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGttt        >  5:1493464/1‑151 (MQ=255)
                                                                                                                                           ggcTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGttt        >  7:117153/1‑151 (MQ=255)
                                                                                                                                               tCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAATGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtct      <  1:1286187/149‑1 (MQ=255)
                                                                                                                                               tCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtct      <  3:30390/149‑1 (MQ=255)
                                                                                                                                               tCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtct      <  2:252830/149‑1 (MQ=255)
                                                                                                                                                 aCTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACCTTGTTGTTTCtcgtc  >  5:1011464/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTGTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTCGTC  >  NC_000913/962210‑962505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑