Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,151,759 G→A E49K (GAA→AAA)  acpP → acyl carrier protein (ACP)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,151,7590GA94.2% 1187.6 / 40.3 344E49K (GAA→AAA) acpPacyl carrier protein (ACP)
Reads supporting (aligned to +/- strand):  ref base G (8/12);  new base A (166/158);  total (174/170)
Fisher's exact test for biased strand distribution p-value = 3.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01

Only 100 of 356 total aligned reads displayed.

AGTATGAGCACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACT  >  NC_000913/1151612‑1151906
                                                                                                                                                   |                                                                                                                                                   
agTATGAGCACTATCGAAGAACGGGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACAGCGTTGAGCTGGTAATGGCTCTGGaaaaag                                                                                                                                                  <  1:1422986/151‑1 (MQ=255)
 gTATGAGCACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAaaaaga                                                                                                                                                 <  8:314993/151‑1 (MQ=255)
  tATGAGCACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGaaaaag                                                                                                                                                  >  5:124830/1‑149 (MQ=255)
   aTGAGCACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAGAagag                                                                                                                                                <  2:816305/150‑1 (MQ=255)
   aTGAGCACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGt                                                                                                                                               <  5:1058368/151‑1 (MQ=255)
   aTGAGCACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGt                                                                                                                                               >  3:83789/1‑151 (MQ=255)
    tGAGCACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGtt                                                                                                                                              >  8:847693/1‑151 (MQ=255)
     gAGCACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAGAAGAGttt                                                                                                                                             <  4:501506/151‑1 (MQ=255)
     gAGCACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAGAAGAGttt                                                                                                                                             <  2:87229/151‑1 (MQ=255)
         aCTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGAt                                                                                                                                          >  4:688134/1‑150 (MQ=255)
          cTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGATGTTACCAACAATGCTTCTTTCGGTGAAGCCCTGGGCGCGGATTCTGTTGACACCGTTGCGCTGGTAATGGCTCTGGAAAATGAGTTTGAt                                                                                                                                          >  3:525239/1‑149 (MQ=255)
          cTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATAc                                                                                                                                        >  8:749028/1‑151 (MQ=255)
            aTCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGCTTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTg                                                                                                                                      >  2:251696/1‑151 (MQ=255)
            aTCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATAc                                                                                                                                        <  8:1195108/149‑1 (MQ=255)
            aTCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACt                                                                                                                                       <  7:709442/150‑1 (MQ=255)
              cGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTGAAGCAGGAAGAAGTTACCAACAATGCTTCGTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGGAATGGCTCTGGAAAAAGAGTTTGATACTg                                                                                                                                      >  1:875312/1‑149 (MQ=255)
                aagaaCGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTg                                                                                                                                      >  2:119295/1‑147 (MQ=255)
                aagaaCGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGAt                                                                                                                                  <  6:1331104/151‑1 (MQ=255)
                aagaaCGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGAt                                                                                                                                  <  7:232604/151‑1 (MQ=255)
                 agaaCGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGCGCGCGCATTCTCTTGACAACGTTGAGCTGGAAATGGCTCTGGAAAAAGAGTTTGATACTGAGAtt                                                                                                                                 >  8:1370123/1‑151 (MQ=255)
                   aaCGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTcc                                                                                                                               <  2:1117430/151‑1 (MQ=255)
                   aaCGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTcc                                                                                                                               <  6:194558/151‑1 (MQ=255)
                    aCGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCg                                                                                                                              <  5:179424/151‑1 (MQ=255)
                      gcgTTAAGATAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATAc                                                                                                                                        <  8:1268262/139‑1 (MQ=255)
                      gcgTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGa                                                                                                                            <  4:1477103/151‑1 (MQ=255)
                       cgTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGAc                                                                                                                           >  6:723963/1‑151 (MQ=255)
                          tAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACga                                                                                                                         >  5:232105/1‑150 (MQ=255)
                           aaGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACgaag                                                                                                                       <  6:312981/151‑1 (MQ=255)
                           aaGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACgaa                                                                                                                        <  7:1342327/150‑1 (MQ=255)
                           aaGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACgaa                                                                                                                        <  3:325472/150‑1 (MQ=255)
                            aGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACgaaga                                                                                                                      <  6:794025/151‑1 (MQ=255)
                            aGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACgaaga                                                                                                                      <  3:6608/151‑1 (MQ=255)
                              aaaaTTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTATGGAAAAAGAGTTTGATACTGAGATTCCGGACGaagaag                                                                                                                    <  5:1314531/151‑1 (MQ=255)
                              aaaaTTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCCGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACgaaga                                                                                                                      >  6:1369240/1‑149 (MQ=255)
                                 aTTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTg                                                                                                                 >  7:1452205/1‑151 (MQ=255)
                                      cGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGaa                                                                                                             <  7:403360/150‑1 (MQ=255)
                                           aaCAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATcac                                                                                                       <  7:532877/151‑1 (MQ=255)
                                              aGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATcaccac                                                                                                    >  1:170787/1‑151 (MQ=255)
                                              aGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATcaccac                                                                                                    >  7:314528/1‑151 (MQ=255)
                                                cTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCg                                                                                                  >  4:612961/1‑151 (MQ=255)
                                                cTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCg                                                                                                  >  1:1013805/1‑151 (MQ=255)
                                                 tGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATcacca                                                                                                     >  7:1128196/1‑147 (MQ=255)
                                                  gggCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCg                                                                                                  <  2:670359/149‑1 (MQ=255)
                                                      gTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCACTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAgc                                                                                            >  8:90347/1‑150 (MQ=255)
                                                       ttAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAgg                                                                                            <  5:580223/150‑1 (MQ=255)
                                                         aaGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCt                                                                                          <  4:474178/150‑1 (MQ=255)
                                                         aaGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTg                                                                                         <  4:1282529/151‑1 (MQ=255)
                                                         aaGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTg                                                                                         >  5:1428638/1‑151 (MQ=255)
                                                          aGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGc                                                                                        >  6:952559/1‑151 (MQ=255)
                                                          aGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGc                                                                                        >  2:189893/1‑151 (MQ=255)
                                                            cAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGcc                                                                                       >  8:1034058/1‑150 (MQ=255)
                                                             aGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCAt                                                                                     >  1:384171/1‑151 (MQ=255)
                                                                aagaagTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTg                                                                                   >  2:1422316/1‑150 (MQ=255)
                                                                 agaagTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGAt                                                                                 <  5:485841/151‑1 (MQ=255)
                                                                      ttACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACAt                                                                            >  3:640640/1‑151 (MQ=255)
                                                                        aCCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCCCTGGAAGAAGAGTTTCATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATc                                                                           >  1:127902/1‑150 (MQ=255)
                                                                           aacaaTGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACg                                                                       <  8:444160/151‑1 (MQ=255)
                                                                            acaaTGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACgg                                                                      <  6:557267/151‑1 (MQ=255)
                                                                             caaTGCTTCTTTCGTTGAAGACCTGGGCGCGGATTGTGTTGAGAGCGTTGAGCTGGTTTTGTTTCTTGATATAGAGTTTGATACTGAGTTTCCGGATGAAGAATCTGAGTAAATCACGACCGTTCAGTCTGCGATTGATTACATCAAGGGc                                                                     <  7:770503/151‑1 (MQ=255)
                                                                                   ttcttTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGccacc                                                                 >  3:886354/1‑149 (MQ=255)
                                                                                    tcttTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGccacca                                                                <  3:1009877/149‑1 (MQ=255)
                                                                                    tcttTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGccacca                                                                <  8:557506/149‑1 (MQ=255)
                                                                                     cttTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGc                                                             >  4:1238157/1‑151 (MQ=255)
                                                                                       ttCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGt                                                           >  4:195638/1‑151 (MQ=255)
                                                                                        tCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGt                                                           >  5:728792/1‑150 (MQ=255)
                                                                                         cGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTa                                                          <  1:1156400/150‑1 (MQ=255)
                                                                                          gTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATTCTGAGATTCCGGACGAAGCAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTa                                                          >  4:995496/1‑149 (MQ=255)
                                                                                               aGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAAc                                                   <  3:41238/151‑1 (MQ=255)
                                                                                                 aCCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACAt                                                 >  7:1246868/1‑151 (MQ=255)
                                                                                                   cTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAg                                                                                             <  8:654943/105‑1 (MQ=255)
                                                                                                   cTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATAACATCAACGGCCACCAGGCGTAAGTGAACAtc                                                >  1:731394/1‑150 (MQ=255)
                                                                                                      ggCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCa                                            <  1:62690/151‑1 (MQ=255)
                                                                                                      ggCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATAGATTACATCAACGGCCACCAGGCGTAAGTGAACAtctac                                             >  2:1357562/1‑148 (MQ=255)
                                                                                                          cgGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACAGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCg                                        >  6:66900/1‑151 (MQ=255)
                                                                                                             aTTCTGTTGACACCGTTGAGCTTGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCTGACGAAGAAGCTGTGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGt                                      <  7:988649/150‑1 (MQ=255)
                                                                                                              ttCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCg                                    >  5:671594/1‑151 (MQ=255)
                                                                                                               tctcTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGt                                   >  5:1151880/1‑151 (MQ=255)
                                                                                                                 tctTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTc                                 >  1:525055/1‑151 (MQ=255)
                                                                                                                    tGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTc                                 <  3:751488/148‑1 (MQ=255)
                                                                                                                    tGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCg                                <  5:925691/149‑1 (MQ=255)
                                                                                                                    tGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGAc                              >  6:932365/1‑151 (MQ=255)
                                                                                                                     gACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGAcc                             <  6:619441/151‑1 (MQ=255)
                                                                                                                     gACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGAcc                             <  6:415477/151‑1 (MQ=255)
                                                                                                                        acCGTTGAGCTGGTAATGGCTCTGGAAACAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGTCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGGTCGACCGcc                          <  7:845889/151‑1 (MQ=255)
                                                                                                                             tGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGttgatgtaatcaat                      <  4:744164/150‑13 (MQ=255)
                                                                                                                             tGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAg                      <  2:456181/150‑1 (MQ=255)
                                                                                                                              gAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGtt                    <  3:994986/151‑1 (MQ=255)
                                                                                                                                gCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAATATCTCCAGGCGGTCGTTCGACCGCCTGAGtttt                  <  2:517515/151‑1 (MQ=255)
                                                                                                                                  tGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTAt                >  6:1169337/1‑151 (MQ=255)
                                                                                                                                   ggTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTAt                <  2:1291905/150‑1 (MQ=255)
                                                                                                                                      aaTGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCAGCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCttt            <  3:1459147/151‑1 (MQ=255)
                                                                                                                                       aTGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGGTCGACCGCCTGAGGTTTATCttat           >  3:1242773/1‑149 (MQ=255)
                                                                                                                                       aTGGCTCTGGAAAAAAAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCtttt           >  8:412418/1‑151 (MQ=255)
                                                                                                                                        tGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCttttt          >  6:1016760/1‑151 (MQ=255)
                                                                                                                                         ggCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGATGAAGCTGAGAAAATCACCACAGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGGCGTTCGACCGCCTGAGTTTTATCTTTTTg         <  7:487714/151‑1 (MQ=255)
                                                                                                                                         ggCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCtttttt         >  2:905064/1‑150 (MQ=255)
                                                                                                                                           ctctGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTc       >  8:639709/1‑151 (MQ=255)
                                                                                                                                             ctGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGACTTTTATCTTTTTGTccc     >  1:949289/1‑151 (MQ=255)
                                                                                                                                              tGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTccc     >  5:874376/1‑150 (MQ=255)
                                                                                                                                                gaaaaaGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACt  >  4:910349/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AGTATGAGCACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACT  >  NC_000913/1151612‑1151906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: