Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913422,4290TG23.6% 795.3 / 88.2 408intergenic (+70/‑89)proY/malZproline‑specific permease/maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (68/240);  new base G (93/2);  total (166/242)
Fisher's exact test for biased strand distribution p-value = 4.34e-44
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 424 total aligned reads displayed.

GTTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGC  >  NC_000913/422280‑422578
                                                                                                                                                     |                                                                                                                                                     
gTTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGGTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCGCGtc                                                                                                                                                      >  8:358990/1‑151 (MQ=255)
  ttCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtcc                                                                                                                                                     <  1:655592/150‑1 (MQ=255)
     gcgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGCCTATTCCGAAAGTTATCCGCGCCCGtcctccg                                                                                                                                                 >  5:1234147/1‑150 (MQ=255)
         ggATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGTCCTCCGcccc                                                                                                                                             >  1:886796/1‑151 (MQ=255)
             tgttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCTGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCaa                                                                                                                                          <  3:485218/150‑1 (MQ=255)
              gttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAat                                                                                                                                        <  2:1489350/151‑1 (MQ=255)
               ttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCCGCGTCCCCCAAat                                                                                                                                        >  7:1231295/1‑150 (MQ=255)
                tgtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAata                                                                                                                                       <  7:353674/150‑1 (MQ=255)
                 gtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGCCCCCAACTATc                                                                                                                                     >  4:1044865/1‑151 (MQ=255)
                     tGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTc                                                                                                                                 >  3:1417706/1‑151 (MQ=255)
                      gTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCtt                                                                                                                                  <  6:1245736/149‑1 (MQ=255)
                      gTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCtt                                                                                                                                  <  6:1277675/149‑1 (MQ=255)
                      gTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCtt                                                                                                                                  <  3:1055743/149‑1 (MQ=255)
                        tGATTGGCTGGATGTTTAAACGCCGCCACGAGCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAg                                                                                                                               <  8:1165941/150‑1 (MQ=255)
                            tGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCCTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGACCACCGACCCCTAAAAGCCGTCacaaga                                                                                                                           >  2:1072195/1‑145 (MQ=255)
                             ggCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgatgat                                                                                                                          <  3:743993/150‑1 (MQ=255)
                             ggCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGatgatg                                                                                                                         <  2:1420139/151‑1 (MQ=255)
                                 ggATGTTTAAACGCCGCCACGATCGTCAGCTGTCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGt                                                                                                                      <  4:863434/150‑1 (MQ=255)
                                 ggATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGt                                                                                                                      <  3:424008/150‑1 (MQ=255)
                                  gatgatTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGTAGGAAATCCTGATCCGTCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGa                                                                                                                    <  8:1633439/151‑1 (MQ=255)
                                  gATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGa                                                                                                                    <  5:1125109/151‑1 (MQ=255)
                                   aTGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGAt                                                                                                                   <  7:11452/151‑1 (MQ=255)
                                      tttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCGCCTGCTCCGCCCCCAACTATCCTTCAGATGATGAGTGATCCt                                                                                                                >  4:706336/1‑151 (MQ=255)
                                       ttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCGCGGCCGCCGCCGCCAAAGATCCTTCAGATGATGGGTGATGCTg                                                                                                               >  5:600692/1‑151 (MQ=255)
                                       ttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGCTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCt                                                                                                                <  2:91606/150‑1 (MQ=255)
                                           aCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGGCCGCCGCCCCCCCAGATCCTTCACATGCTGAGGGATccgggagt                                                                                                           >  4:41301/1‑145 (MQ=255)
                                           aCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCAtt                                                                                                           <  1:112087/151‑1 (MQ=255)
                                              ccgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGATCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAgg                                                                                                        <  6:1392727/151‑1 (MQ=255)
                                              ccgcaACGATCGTGAGCTCGCTGAAAACCTTTTATCCTTGGGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTGCGAAAGTTATCGGCCGCCGTCCTGCTCCCGCATATATCCTTCAGATGATGAGTGATCCCGCATTAgg                                                                                                        <  3:577080/151‑1 (MQ=255)
                                                gccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGACCACCGCCCCCACAAAACCTTAAGATGATGGGTgttagggctttaggc                                                                                                       >  5:757744/1‑136 (MQ=255)
                                                gccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCAAAAGTTATCCGCGCCCGGCCTCCGCCCCCCAAGATCCGTCAGGGGATGAGGGATCCTgggtgagggg                                                                                                      >  7:803793/1‑142 (MQ=255)
                                                  cACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAAAAACCTTCAGCTGATGAGGGATCCTGCGTTAGGCTAt                                                                                                    >  1:1427911/1‑151 (MQ=255)
                                                          tCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATACTTCAGATGATGAGTGATGCTGCAGTAGGCTATGGGAAGGt                                                                                            >  5:513851/1‑151 (MQ=255)
                                                          gcAGCTGGCTTTAATCCAGTAATGCTTGCGTTCTGTTAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGt                                                                                            <  2:1797173/150‑1 (MQ=255)
                                                           cAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCACATAACCAGCCGATGATGAGGGCTCCGGCCTGAGTGTATGGCAAgggg                                                                                           >  2:519664/1‑149 (MQ=255)
                                                              ctggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGGCGGGGCGCCGGCGCCCCATCTCCGTCCGCTGATGAGTGATCCTGCATTAGGCGATGGCAAGGGGagg                                                                                        >  7:97751/1‑149 (MQ=255)
                                                                ggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATc                                                                                        <  7:576356/149‑1 (MQ=255)
                                                                ggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGCCCCCAAATATCCTTCAGATGTTGAGTGATCCTGCATTAGGGTATGGAAAGGTGATc                                                                                        >  6:685360/1‑149 (MQ=255)
                                                                 gctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGa                                                                                     <  1:316414/151‑1 (MQ=255)
                                                                    gAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAtt                                                                                   <  8:765841/150‑1 (MQ=255)
                                                                    gAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAt                                                                                    <  1:535912/149‑1 (MQ=255)
                                                                    gAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAt                                                                                    <  6:911682/149‑1 (MQ=255)
                                                                      aaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGGCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGGATTAGGCTATGGGTAGGGGATCAGATTTtc                                                                                >  1:1725543/1‑151 (MQ=255)
                                                                       aaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtca                                                                               <  6:1793908/151‑1 (MQ=255)
                                                                          cAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGGTCCTGCATTAGGCTATGGCAAGGTGATCAGATTTttatc                                                                             >  4:1445198/1‑150 (MQ=255)
                                                                          cAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCCACAATCCGTCGGAAGATGAGGGATCCTGCAAGAGGGTATGGCAAGGTGAGCAGCTTTtcata                                                                             >  8:652779/1‑149 (MQ=255)
                                                                           aGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATcac                                                                           <  7:168129/151‑1 (MQ=255)
                                                                            gTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATcaca                                                                          <  6:169105/151‑1 (MQ=255)
                                                                              aaTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAg                                                                         <  7:1727395/150‑1 (MQ=255)
                                                                                tCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGGCCGGGCGCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGGAGTAGGCTATGGCAAGGTGATGAGATTTTCTTCACAgggg                                                                      >  1:1546320/1‑151 (MQ=255)
                                                                                 ccTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGa                                                                     <  3:1546499/151‑1 (MQ=255)
                                                                                  cTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAAAATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGGGATCAGATTTTGTTAAAAGGGGat                                                                    >  3:1737905/1‑150 (MQ=255)
                                                                                    tGCGGTCTGTAAGCCGGGTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAAt                                                                   <  2:296942/150‑1 (MQ=255)
                                                                                     gCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTa                                                                 <  7:445283/151‑1 (MQ=255)
                                                                                       gtgcTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatg                                                               <  4:1196524/148‑1 (MQ=255)
                                                                                       gTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCAGAGGGGAATTatg                                                               <  1:1381287/151‑1 (MQ=255)
                                                                                         tCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCGCCCCCAAAAAACCATCAGAGGAAGAGGGCTCCTGAATTAGGGAGAGGGAAGGTGATCTGATTTTCATCACAGGGGAATTttgat                                                             >  4:182119/1‑151 (MQ=255)
                                                                                           tGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCGGCGGCCGGCGCGCCCGGAGAGCCGGCAGGTGATGAGTGAGGCTGGAGTAGGCGGTGGCTAGGGGATGAGGGGTTGATGTCAGGGGTATTATGATGt                                                           >  5:916969/1‑151 (MQ=255)
                                                                                            gTAAGCCGGTTCAGGATGAAATCCTGATGCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTGCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgat                                                             <  5:141317/148‑1 (MQ=255)
                                                                                            gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgat                                                             <  6:220808/148‑1 (MQ=255)
                                                                                              aaGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTa                                                         <  2:1244486/150‑1 (MQ=255)
                                                                                                   ggTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCa                                                   <  5:1621899/151‑1 (MQ=255)
                                                                                                   ggTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCa                                                   <  5:442654/151‑1 (MQ=255)
                                                                                                      tCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCTCCTCCCCCAAATATGCTTAAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTGATCAAAGGGGAATTATGATGTTTAATTCATgg                                                >  1:1007123/1‑151 (MQ=255)
                                                                                                      tCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATg                                                 <  8:243252/150‑1 (MQ=255)
                                                                                                      tCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATg                                                 <  3:1153219/150‑1 (MQ=255)
                                                                                                      tCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCAAATATACTTCAGATGATGAGTGATCCAGCATTAGGCTATGGCAAGGTGAGCAGATAATAAAAAAACGGGAATTATGATGTTTAATGCATgc                                                >  8:1783914/1‑150 (MQ=255)
                                                                                                       cAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGc                                               <  8:546731/151‑1 (MQ=255)
                                                                                                       cAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGc                                               <  4:1375448/151‑1 (MQ=255)
                                                                                                                aTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCgg                                      <  2:1659346/151‑1 (MQ=255)
                                                                                                                 tCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCCGCCTCCCCCCAAAAACAGACAGATGATGAGTGATCCTGGAGTAGGCTATGGCATGGTGATCAGATTTTGATAACAGGGGAATTATCATGATAGATGAATggaagatgcaggt                                     >  3:965118/1‑140 (MQ=255)
                                                                                                                  ccTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATGACAGGGGATTTATGTTGTTAAATGCATGGCACCTGCCGGTg                                    >  3:195666/1‑151 (MQ=255)
                                                                                                                     gATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGc                                   <  4:1676324/149‑1 (MQ=255)
                                                                                                                     gATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTAAGATGATGAGTGATCCTGCATTAGGCTATGGGAAGGTGATGAGATTTTGATCACACGGGAATTATGATTTTAAATTCATGTAACCTGCCGGTGccc                                 >  1:1894359/1‑151 (MQ=255)
                                                                                                                      aTCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAAAATCCTTCAGATGATGAGTGATAATGCATTATGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATTAATGGAAACTCCCGGTGcccc                                >  8:855184/1‑151 (MQ=255)
                                                                                                                        ccGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTCCCCCCa                              <  1:757274/151‑1 (MQ=255)
                                                                                                                        ccGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCAATAGGCTATGGCAAGGTGGTAAGATTTTAATCACAGGGGAATTATGATTTTAAATTCATTTCACCTGCCGGTGccccc                               >  6:334006/1‑150 (MQ=255)
                                                                                                                         cGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGt                                                                                            <  6:463310/88‑1 (MQ=255)
                                                                                                                          ggCTATTCCGAAAGTTATCCGCGCCCCGCCGCCGCCCCCAAAAATCATTCAGATGATGAGTGATACTGCAATAGGCTATGGGAAGGTGATAATAGATTCATAAAAAGGGAATTATGATGTTAAATGAATGTCACCTGCAGGTGCCCCCaat                            >  2:1319201/1‑149 (MQ=255)
                                                                                                                           gCTATTCCGAAAGTTATCCGGCCCCGGCCGGCGCCCCCACATAGCATACAGAGGATGAGTGAGCCTGCATTAGGGTATGGAAGGGTGATCAGAGTTTCAACAGAGGGGAATAATTATGTTAAATGAATGGAACGTGCGGGGGGCCCCaagt                           >  2:563110/1‑148 (MQ=255)
                                                                                                                           gCTATTCCGAAAGTTATCCGCGCCCGGCCGCCGCCCCCCCAAAGGCTTGACATGAGGAGTGCTCCTGGATTAGGGGATGGGAAGGTGGTCAGATTTTAATCTCAGGGGATGTATGATGTTTAATGAATGGCAGCGGCGGGGGCCCCCaggt                           >  5:1822874/1‑148 (MQ=255)
                                                                                                                           gCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATGCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCAttt                           <  3:1101640/151‑1 (MQ=255)
                                                                                                                           gCTATTCCGAAAGTTATCCGCCCCCGTCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGATCATGCATTAGGCTATGGCAAGGTGCTCAGATTTTAATCACAGGGGAATTATGATTTTAAATTCATTTAAAAAGCCGGTGCCCCCAatt                           >  4:383284/1‑151 (MQ=255)
                                                                                                                            gtATTCCGAAAGTTATCCTCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTg                          <  8:1297372/150‑1 (MQ=255)
                                                                                                                            gtATTCCGAAAGTGATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTg                          <  4:1123514/150‑1 (MQ=255)
                                                                                                                                 ccGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTaa                      <  8:1050740/150‑1 (MQ=255)
                                                                                                                                 ccGAAAGTTATCCGCCCCCGTCCTCCGCCCCCAAAAATACTTCAGATGATGACTGATCCAGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCAGCACCTGCCCCCATTTGTTaa                      >  6:833010/1‑150 (MQ=255)
                                                                                                                                   gAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGGATTAGGGTATGGCAAGGTGATCAGATTTTGATCACAGGGGAATTTTGATGTTTAATGCATGGCACCGGCCGGGGCCCCCCGTTGTTaaaa                    >  2:1583806/1‑149 (MQ=255)
                                                                                                                                    aaaGTTATCCGCCCCCGTCCTCCTCCCCCAAAAATCGTAAAAATGTGGAGTGAGCGGGCATTTGGGTATGGCAAGGGGTGAAGATTTTAAAAACAGGGGAATTATAATGTTAAATGAATGCCCCCTCCCGGGGCCCCCATTTGTTaaaaa                   >  8:1860371/1‑148 (MQ=255)
                                                                                                                                      aGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaag                <  1:1811457/151‑1 (MQ=255)
                                                                                                                                         tATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagca              <  7:214101/150‑1 (MQ=255)
                                                                                                                                         tATCCGACCCCGTGCTCCTGCACCAAATATCCTTGAGTTGATGAGTGATCCTGCATTAGGGTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGGATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagca              <  3:65965/150‑1 (MQ=255)
                                                                                                                                              gCCCCCGTCCTACTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATc        <  4:570436/151‑1 (MQ=255)
                                                                                                                                                ccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCaa      <  7:913966/151‑1 (MQ=255)
                                                                                                                                                ccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCa       >  7:1401533/1‑150 (MQ=255)
                                                                                                                                                ccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCa       >  6:109398/1‑150 (MQ=255)
                                                                                                                                                 cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAAc     >  7:231822/1‑151 (MQ=255)
                                                                                                                                                  ccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt    <  6:1187801/151‑1 (MQ=255)
                                                                                                                                                   cGTCCTCCTCCCCCAAATATCCTTGAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTg   <  2:744285/151‑1 (MQ=255)
                                                                                                                                                   cGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTg   >  3:1304160/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGC  >  NC_000913/422280‑422578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑