Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,818,1450TC23.3% 359.1 / 128.1 243intergenic (‑10/+53)argZ/argYtRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base T (61/94);  new base C (6/41);  total (103/140)
Fisher's exact test for biased strand distribution p-value = 6.55e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.40e-03

Only 100 of 275 total aligned reads displayed.

CTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACT  >  NC_000913/2817995‑2818289
                                                                                                                                                      |                                                                                                                                                  
  agagCCTGAGGTTATTTCCTCTACCTCTTGCATACCTTCATTGCTGAAGATTTCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAatt                                                                                                                                                   <  4:169195/147‑1 (MQ=14)
  aGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAattt                                                                                                                                                  <  5:1796103/151‑1 (MQ=40)
  aGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAatt                                                                                                                                                   <  4:910289/150‑1 (MQ=40)
  aGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAatt                                                                                                                                                   <  1:1374787/150‑1 (MQ=40)
  aGTGCCTGAGGTTAATTCCGCTTCCTCTTCGATACCTTCATTGCTGAAGATTTCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAat                                                                                                                                                    <  8:862091/149‑1 (MQ=255)
     gCCTGCGGTTAATTCCTCTACCTCTTCGATACCTTCATTGCTGAAGAATTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAatttatt                                                                                                                                               <  3:1590764/151‑1 (MQ=255)
      cgttcttttATTTCCTCTTCCTCTTCTATCCGTTCATTCATGAATATTTCGTACTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAacttactt                                                                                                                                              <  5:139304/144‑1 (MQ=11)
       cTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTActtt                                                                                                                                             >  7:1493509/1‑151 (MQ=255)
         gCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTAtttt                                                                                                                                             <  8:118054/149‑1 (MQ=255)
          cGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTAct                                                                                                                                          <  1:1522507/151‑1 (MQ=255)
           ggTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTAGTTTActg                                                                                                                                         >  1:479406/1‑151 (MQ=255)
             tttaTTCCTCGACCTCTTCCATACCCTCATTCCTGAAGATTTCGGACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTActgc                                                                                                                                        <  2:1119328/147‑1 (MQ=14)
               atttcctcgagctctTCGAAACCTTAATTGCTCAATATGACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGa                                                                                                                                     <  6:1613685/140‑1 (MQ=11)
               atttCCTCTACATCTTCCAAACGATAATTCCTGAATATTTCGGAGTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGa                                                                                                                                     <  5:1037069/149‑1 (MQ=11)
               aaTTCCGCTACCTCTTCTATACCTTCATTGCTGAAGATTTCGGATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGa                                                                                                                                     <  8:209840/151‑1 (MQ=255)
               aaTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgctg                                                                                                                                      <  5:1268810/150‑1 (MQ=255)
               aaTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGa                                                                                                                                     <  7:1370931/151‑1 (MQ=255)
                aTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGa‑‑t                                                                                                                                  <  4:1835317/151‑1 (MQ=255)
                  tCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGa‑‑ta                                                                                                                                 <  6:1430031/150‑1 (MQ=255)
                  tCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAATTAATTTACTGCAGa‑‑tat                                                                                                                                >  1:56216/1‑151 (MQ=255)
                   ccGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TAtt                                                                                                                               <  1:157480/151‑1 (MQ=255)
                   ccGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTAGTTTACTGCAGA‑‑TAtt                                                                                                                               >  6:68177/1‑151 (MQ=255)
                    cGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCCCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTATTTTACTGCTGA‑‑TAttt                                                                                                                              >  7:743754/1‑150 (MQ=255)
                     ggtagatctTCGATACGTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGA‑‑tttttt                                                                                                                             <  8:1491037/145‑3 (MQ=11)
                     gCTACCTCTTCGATACCTTCATTGCTGAAGATTTCTGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCAAATACTATATCCCTCTTAGCTAGCGATTCAGAGGGAAAATTATTTTAATGCTGA‑‑TATTg                                                                                                                              >  3:1421369/1‑150 (MQ=14)
                     gCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGATCTACGGATCCATCTGGAAATTTATTTTACTGCTGA‑‑TATTg                                                                                                                              >  8:55793/1‑150 (MQ=255)
                     gCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTAATTTACTGCAGa‑‑gatagc                                                                                                                             >  1:1058856/1‑148 (MQ=255)
                      gtACATCTTCGATACCTTCATTCCTGAATATTTCGTATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGA‑‑ttttttg                                                                                                                            <  5:328985/150‑4 (MQ=11)
                      gatccTCTTCGAAACCATCATAGCTGAATATGTAGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGA‑‑ttttt                                                                                                                              <  7:955478/146‑2 (MQ=11)
                       tACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTAGTTTAATGCTCA‑‑TATTGAt                                                                                                                            >  8:109167/1‑150 (MQ=255)
                       tACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAATTAATTTACTGCTGA‑‑TATTTAt                                                                                                                            >  3:1182132/1‑150 (MQ=255)
                       cacctcccccaaaCCTTCATTTATTAACATGACGCAGAATAATGTGCATCCGGCCTTATTCGAAGGTCCGACCGCGGGGTTCGTATCCGAGCTCTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATa                                                                                                                           <  6:612171/139‑1 (MQ=2)
                        aCCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATa                                                                                                                           <  2:436805/150‑1 (MQ=255)
                        aCCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATa                                                                                                                           <  1:899335/150‑1 (MQ=255)
                        aCCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATAc                                                                                                                          <  5:1676851/151‑1 (MQ=255)
                        aCCTCTTCCATACCATCATTCCTGCAGATATCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGA‑‑ttttttgat                                                                                                                          <  4:1072064/151‑6 (MQ=11)
                         ccTCTTCCCTACCTTCCTTGCTGCAGATTTCGGAGAATATGGTGCTTCTTTGAGGATTCGAACCTCCGTCCTGTTTCTTAGTAGCCGAGTACTCTATCCACCTGTTCTAGGTATGGATCTGTAAATTTATTTTACTGCTGA‑‑TATTGATAcc                                                                                                                         <  2:854940/151‑1 (MQ=255)
                              tCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGcta                                                                                                                     <  6:1587593/150‑1 (MQ=255)
                              tCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGct                                                                                                                      <  1:755379/149‑1 (MQ=255)
                               cGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGctac                                                                                                                    <  5:1169030/150‑1 (MQ=255)
                                catcccTTCATTGCTGACGATTTCGGAGAATATGGTGCATCCTTTTTTATTCGAACCTCCGACCTCTCTGTTCTTATCATAGTACTATATTCTTCTGATATACGTATCTATCGGGATATTTATTTTACTGCTGA‑‑TATTGATACCGctacta                                                                                                                  <  8:1718644/147‑1 (MQ=9)
                                 aTACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTaa                                                                                                                 <  4:501425/151‑1 (MQ=255)
                                 aTACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTaa                                                                                                                 <  5:548404/151‑1 (MQ=255)
                                 aTACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACAGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATAGGGAAATTTATTTTACTGCAGA‑‑TAtttagatacctcata                                                                                                                  >  1:1077851/1‑137 (MQ=14)
                                  tACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgctg                                                                                                                                      >  4:1555791/1‑131 (MQ=18)
                                   aCCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGTATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTaaa                                                                                                                <  6:1397272/150‑1 (MQ=255)
                                   aCCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTaaa                                                                                                                <  6:1775772/150‑1 (MQ=255)
                                   aCCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAg                                                                                                               <  3:567018/151‑1 (MQ=255)
                                   aCCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCGCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAg                                                                                                               <  2:180496/151‑1 (MQ=255)
                                     gctcttactcgcttaTTTAGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAg                                                                                                               <  5:689083/137‑1 (MQ=14)
                                     cTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTTCTACCGCTTAGAAAGcc                                                                                                             >  6:779557/1‑151 (MQ=11)
                                     cTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGATCCGTTCCTCGCCGAGGACTCTATCCAGGTCAGCTACGGCTGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAACGcc                                                                                                             >  4:643843/1‑151 (MQ=14)
                                      ttCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTATTTTACTGCTGA‑‑TTTTTATAAAGCTAGTAAAGcgc                                                                                                            >  7:1270381/1‑149 (MQ=14)
                                       tCATTCCTGAATATTTAGGATTATATGGTGCATCCGGGAGGATTCGAACCTCCGAGCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAg                                                                                                               <  2:235978/149‑1 (MQ=14)
                                        cATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCata                                                                                                          <  6:532273/151‑1 (MQ=255)
                                        agttgatcaatatatCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGcc                                                                                                             <  1:1804052/136‑1 (MQ=14)
                                         cgcgcgccccctggcGGAGAATTTTTTGAATCCGGCACGAGTCTAACCTCCGCCCGATCGGGTCGTGTCGTACTACGGTTTCCATTTCCGCTTGGCATGCTTCGGCAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCatat                                                                                                         <  7:29361/137‑1 (MQ=2)
                                           tGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATc                                                                                                        <  5:953555/150‑1 (MQ=255)
                                           agcggaatatataggAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCgta                                                                                                          <  7:1444575/137‑1 (MQ=14)
                                            tatGAATATGTCGTATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCgtat                                                                                                         <  8:1268300/148‑1 (MQ=14)
                                            gCTTAAGTTTTCGTATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTTAGCTACGTATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCa                                                                                                       <  3:950309/150‑1 (MQ=14)
                                            actcaaGATTTAGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATc                                                                                                        <  7:1540863/147‑1 (MQ=14)
                                               gAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGt                                                                                                    <  1:1165357/150‑1 (MQ=255)
                                               gAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGt                                                                                                    <  6:430722/150‑1 (MQ=255)
                                                 aGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATAAAGCTAATAAACCCATATCAATTatt                                                                                                 >  1:371446/1‑149 (MQ=14)
                                                  gATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTAGTTTACTGCTGA‑‑TATTTAGAGCCCTATTAAAgagctctctaatata                                                                                                 >  4:1386404/1‑136 (MQ=14)
                                                   ccccccgcgaacctGGTCCATCCGCGAGGATTCGAATCTTCTACCGATCGGTTCCTAGCCGAGTACTCTATCCAGCGGACCTACGGTTGCATCGCGTAATCTATTTTTATGTTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAagag                                                                                               <  8:668488/138‑1 (MQ=2)
                                                      tCGGAGATTTTTTTGCATCCTGGAGGATTTGACCCTCGGACGGGTCGGTTCGTAGCGTAGTAGTCTATCCAGCTGAGCTACGGATGCATCTGTAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGAt                                                                                             <  8:416875/150‑1 (MQ=14)
                                                       cGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTAGTTTACTGCAGa                                                                                                                                     >  4:410082/1‑111 (MQ=9)
                                                         gagaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTg                                                                                         <  7:888417/151‑1 (MQ=255)
                                                               atGGTTCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTATCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATcc                                                                                    <  1:215940/150‑1 (MQ=255)
                                                               atGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATcc                                                                                    <  6:132524/150‑1 (MQ=40)
                                                                        ccGGGAGGATTAGAACCTAAGACCGATCGGTTCGTAGCCTAGTAATATATACAGCTGAGCTACGGATGAATCGGGAAATTTATTTTACTGCTGA‑‑TTTTTATAAAGATAAAAAAAAGATATCTAGATATAGATGGTGAATAAGGGAGGtttt                                                                          >  3:720437/1‑150 (MQ=255)
                                                                        ccGGGACGACTCCAACCTCCGCCCGCGCCGCTCGTAGCCGAGTACTCTATCCAGCTGCGCTACGTACGCAGCGGGAAATTTAATTTACTGCTGA‑‑TATTCAAACCCCTACTACAGCCATATCAAGTACCAGATGGTGCATCCGGGAGGATTc                                                                          <  5:1504803/151‑1 (MQ=14)
                                                                         cGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACTGATGCATCGGGAAATTTATTTTACTGGTGA‑‑TATTGATACAGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCg                                                                         >  2:541208/1‑151 (MQ=255)
                                                                         cGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCg                                                                         <  1:956622/151‑1 (MQ=40)
                                                                         cGGGAGGATTCGAACCTCCGACC‑CTCGGTTCCTAGCCGACTACTCTATCCAGCTGAGCAACCGATGCAACGGGAAATTTAATTTACTGCTGA‑‑TATTTATAACGCTACTAAAACCAAATCAACTAAGAGATGGTGCATCCGGGAGGAtt                                                                           >  5:881117/1‑148 (MQ=11)
                                                                          gggAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgct                                                                                                                                       >  6:86661/1‑90 (MQ=38)
                                                                          gggAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGggagagatcgc                                                                        >  4:1244582/1‑144 (MQ=255)
                                                                          gggAGGATTAGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAATTAATTTACTGCAGA‑‑TATTTATAAAGCTAGTATAGAGAGATAAAAAAAGAGATCATGGATCAGggcggcgtgg                                                                         >  3:324446/1‑145 (MQ=0)
                                                                            gaggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGTTGCATCCGGGAGGAttatacc                                                                      >  7:1004450/1‑146 (MQ=255)
                                                                            gaggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAATTATTTTACTGCTGA‑‑TATTGATAACCCTAATAAAGCCATATAAATTAAAAGATCGTGAAtaagcgagaataataa                                                                       >  8:1413373/1‑135 (MQ=11)
                                                                              ggaTTCGAACCTCCGACCGCTTTTTTCTTATCATAGTACTATATCCATCTGATATACTTATTGATAGTGAAATTAATTTTACTCCTGA‑‑TATTGCATCCCCTCCTACATCAATATCAAGTAAAAGATGGTGCATCCGGGAGGATTCGAACCt                                                                    <  3:643614/151‑1 (MQ=9)
                                                                               gaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACAGCTACTAAAGAGATATCAAGTAAGAGATCGTGCATCCGGGAGGATTAGacgcgc                                                                   >  6:315640/1‑146 (MQ=14)
                                                                                 ttCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAattta                                                                                                                                                 <  8:1526861/73‑1 (MQ=33)
                                                                                   cGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATAGCGCTACTAAAGCCATCTCAAGTAAGAGATGGTGCATCCGGGAGGTTTCGACccgccg                                                                 >  7:1243670/1‑149 (MQ=255)
                                                                                        cTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGAATCCGGGAGGATTCGACGCGCCGACCGcac                                                          >  7:444464/1‑149 (MQ=255)
                                                                                           cGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTTATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGACGCTCCGAgcgccgcgc                                                       >  6:735180/1‑146 (MQ=14)
                                                                                            gACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAatttat                                                                                                                                                <  5:155298/63‑1 (MQ=35)
                                                                                                gcgctgTTCGTAGCGGATTACGCTATCCCGCTGCGCTACGTATGCATGGGCAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTa                                                  <  6:1235023/146‑1 (MQ=18)
                                                                                                  tCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTAATTTACTGCTGA‑‑TATTGATACCGCTAAAAAAGCCAGATCAAGGAAGAGATGCAGGATCCTCGAGGATAAGAACAGACGACCGCtacgggcgtagc                                                >  7:240487/1‑140 (MQ=14)
                                                                                                                aGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGCGAGGATTCGAACCTCCGAGCGCGAGGATCGCCGCCGAGCAcacatccc                                  >  4:229788/1‑144 (MQ=17)
                                                                                                                   actctttactgcaccgCTACGGAGGCATCGGCAAAGTTATATTCCGCCTGC‑‑TCTTCATACCGGTCCTATAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGc                               <  4:273113/136‑1 (MQ=17)
                                                                                                                   aCTCTATCCAGCTGAGCTACGGATGCATCGGGAAat                                                                                                                                                    >  2:1401559/1‑36 (MQ=32)
                                                                                                                   aCTCTATCAAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTACAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGc                               <  7:55971/151‑1 (MQ=255)
                                                                                                                      ctATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGAATCAGCGAGGATTAGACGCGCCGAGCGGCGCGCTCGTCGCCGAGTAGAAAATCCAGCttt                            >  7:1104452/1‑149 (MQ=14)
                                                                                                                            aGCTGATCTACGGATGCATCGGGAAATTTATTTTACTGATGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACg                      <  7:1693653/151‑1 (MQ=255)
                                                                                                                                gAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGAt                   <  7:109554/150‑1 (MQ=255)
                                                                                                                                     aCGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGAt                   >  5:727206/1‑145 (MQ=255)
                                                                                                                                                gcgttctttatttTGCTGTTGA‑‑TATTGATACCGCTACTATAGGCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAacttac   <  3:370419/144‑1 (MQ=18)
                                                                                                                                                      |                                                                                                                                                  
CTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACT  >  NC_000913/2817995‑2818289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑