Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,818,1580TA24.4% 342.5 / 113.9 225intergenic (‑23/+40)argZ/argYtRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base T (77/93);  new base A (15/40);  total (92/133)
Fisher's exact test for biased strand distribution p-value = 1.87e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.50e-01

Only 100 of 241 total aligned reads displayed.

TAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTT  >  NC_000913/2818009‑2818305
                                                                                                                                                      |                                                                                                                                                      
tAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTAGTTTActgctg                                                                                                                                                       >  6:717718/1‑151 (MQ=255)
 atttcctcgagctctTCGAAACCTTAATTGCTCAATATGACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGa                                                                                                                                                      <  6:1613685/140‑1 (MQ=11)
 atttCCTCTACATCTTCCAAACGATAATTCCTGAATATTTCGGAGTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGa                                                                                                                                                      <  5:1037069/149‑1 (MQ=11)
 aaTTCCGCTACCTCTTCTATACCTTCATTGCTGAAGATTTCGGATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa                                                                                                                                                      <  8:209840/151‑1 (MQ=255)
 aaTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa                                                                                                                                                      <  6:1746205/151‑1 (MQ=255)
 aaTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa                                                                                                                                                      <  7:1370931/151‑1 (MQ=255)
   ttCCGGTACATCTTCTATACGTTACTTCCGGAATATTTCGTACTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGa‑‑t                                                                                                                                                   <  2:564360/150‑1 (MQ=11)
    tCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑ta                                                                                                                                                  <  6:1430031/150‑1 (MQ=255)
     cctcTACATCTTCCATACCTTAATACCTGAATATTTAGTATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑tttt                                                                                                                                                <  6:1341852/148‑1 (MQ=11)
     ccGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TAtt                                                                                                                                                <  1:157480/151‑1 (MQ=255)
      cGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTg                                                                                                                                               <  6:565548/151‑1 (MQ=255)
       gttatctctTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTg                                                                                                                                               <  1:292099/145‑1 (MQ=9)
       ggtagatctTCGATACGTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑tttttt                                                                                                                                              <  8:1491037/145‑3 (MQ=11)
       gCTACCTCTTCGATACCTTCATTGCTGAAGATTTCTGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCAAATACTATATCCCTCTTAGCTAGCGATTCAGAGGG‑AAAATTATTTTAATGCTGA‑‑TATTg                                                                                                                                               >  3:1421369/1‑150 (MQ=14)
       gCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGATCTACGGATCCATCTGG‑AAATTTATTTTACTGCTGA‑‑TATTg                                                                                                                                               >  8:55793/1‑150 (MQ=255)
       gCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTATTTTActgcttcatctg                                                                                                                                                 >  6:503894/1‑143 (MQ=255)
       gCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTAATTTACTGCAGa‑‑gatagc                                                                                                                                              >  1:1058856/1‑148 (MQ=255)
        gtagatcttgcaTACCTTCATATCTGAATATTTAGTACTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑tttttt                                                                                                                                              <  5:596157/139‑3 (MQ=11)
         tACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTAATTTACTGCTGA‑‑TATTTAt                                                                                                                                             >  3:1182132/1‑150 (MQ=255)
          aCCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATa                                                                                                                                            <  7:249033/150‑1 (MQ=255)
          aCCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTACTTTACTGCAGA‑‑tttttaga                                                                                                                                            >  3:836723/1‑146 (MQ=14)
          aCCTCTTCGATACCTTCATTGCTGAAGATTTCGGACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑ttttttgat                                                                                                                                           <  5:1256438/151‑6 (MQ=14)
           gctctTGCATACCGTCATAGCTGCATATTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑tttttttat                                                                                                                                           <  4:1119392/149‑6 (MQ=11)
           ccTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTTAATCCGCGAGGATTCGAACCTCCGACCGCTCGCTTCGTAGCCGAGTACTCTATCCACCTGAGATACGTATTCATCGGT‑AAATTTATTTTACTGCTGA‑‑TATTGATAcc                                                                                                                                          <  5:1544582/151‑1 (MQ=14)
            ctctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATAGGT‑AAATTTATTTTACGTCTGA‑‑TATTGagtcac                                                                                                                                         >  7:71466/1‑146 (MQ=14)
            ctctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTACAAGCATAGAGGCAACGCATACAACACGAATACTCTAACAAGATGAGATACGTATGCATCGAT‑ATTTTTATATTACTGCTGA‑‑TAttttaaaa                                                                                                                                          >  8:720920/1‑144 (MQ=2)
              attCGAAACCATCATTCCTCAATATAACGTATTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑ttttttgataccg                                                                                                                                       <  8:1551638/150‑10 (MQ=14)
               ttCGATACCTTCATTGCTGACGTTTTCGGATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCg                                                                                                                                         <  2:1141756/148‑1 (MQ=255)
                tCGATACCTTCATTGCTGAAGATTTCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑ttttttgataccg                                                                                                                                       <  6:1009445/149‑10 (MQ=11)
                tCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGct                                                                                                                                       <  1:755379/149‑1 (MQ=255)
                 cGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGctac                                                                                                                                     <  4:1491368/150‑1 (MQ=255)
                 cGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTATTTTACTGCTGA‑‑TATTTAGAACGctaa                                                                                                                                     >  7:126121/1‑149 (MQ=255)
                   aTACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACAGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATAGGG‑AAATTTATTTTACTGCAGA‑‑TAtttagatacctcata                                                                                                                                   >  1:1077851/1‑137 (MQ=14)
                    tACCTTCATTTCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTaa                                                                                                                                  <  5:1861131/150‑1 (MQ=255)
                    tACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTtata                                                                                                                                            >  8:1221330/1‑140 (MQ=16)
                    tACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATa                                                                                                                                            <  7:1223359/140‑1 (MQ=18)
                     aCCTTCATTGCTGAAGTTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAg                                                                                                                                <  8:1017129/151‑1 (MQ=255)
                     aCCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTACTTTACTGCAGA‑‑tttttttaaacctataata                                                                                                                                 >  3:365689/1‑135 (MQ=14)
                      ccTTCATTGCTGAATATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGCTACTaaa                                                                                                                                 <  3:546164/151‑1 (MQ=11)
                       gctcttactcgcttaTTTAGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAg                                                                                                                                <  5:689083/137‑1 (MQ=14)
                       cTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTAAATCTTA‑‑TATTTATACCGCTAATAAAGcc                                                                                                                              >  4:951181/1‑151 (MQ=11)
                       cTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGATCCGTTCCTCGCCGAGGACTCTATCCAGGTCAGCTACGGCTGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAACGcc                                                                                                                              >  4:643843/1‑151 (MQ=14)
                         tactacctccaGATGTAGGACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAg                                                                                                                                <  7:442637/139‑1 (MQ=14)
                         tCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGcc                                                                                                                              <  6:800467/149‑1 (MQ=255)
                         tCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTAATTTACTGCTGA‑‑TATTTATAAAGCTAGAAAAGcga                                                                                                                             >  2:346796/1‑148 (MQ=11)
                         tCATTCCTGAATATTTAGGATTATATGGTGCATCCGGGAGGATTCGAACCTCCGAGCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAg                                                                                                                                <  2:235978/149‑1 (MQ=14)
                          cATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCata                                                                                                                           <  4:1057338/151‑1 (MQ=255)
                          agttgatcaatatatCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGcc                                                                                                                              <  1:1804052/136‑1 (MQ=14)
                             tGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTAGTTTACTGCTGA‑‑TATTTATATAGCTAGTaaaacgagata                                                                                                                         >  6:1840023/1‑142 (MQ=14)
                             catgtcataTTTCGTATAATATGGTGCATCCGGGAGTATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCgtat                                                                                                                          <  8:877174/143‑1 (MQ=14)
                              actcaaGATTTAGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATc                                                                                                                         <  7:1540863/147‑1 (MQ=14)
                                  aaGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTAATTTACTGCTGA‑‑TATTTATACCCCTCCTAAAGCCAGATCTAGTa                                                                                                                    >  7:878691/1‑150 (MQ=14)
                                   ttcctttgtagaATATGGTGCATCCGGGAGTATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTaa                                                                                                                   <  2:485110/141‑1 (MQ=255)
                                         cGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGCCCCATCGGTTCGTAGCCGAGTACTCTATCAAGCTGAGCTACGGATGCATCGGG‑AAATTTAGTTTACTGCTGA‑‑TATTGAGAGCGCTCCTAAAGAGATCTATAGTAAgagagtg                                                                                                            >  1:848464/1‑148 (MQ=14)
                                         cGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTAGTTTACTGCAGa                                                                                                                                                      >  4:410082/1‑111 (MQ=9)
                                           gagaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTg                                                                                                          <  5:778984/151‑1 (MQ=39)
                                            agaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑tat                                                                                                                                                 <  3:1605300/111‑1 (MQ=40)
                                            agaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGc                                                                                                         <  2:1541510/151‑1 (MQ=39)
                                             gaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCa                                                                                                        <  1:1199464/151‑1 (MQ=39)
                                                        aTCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTTATAAACCTACTAAAAACAAATAAAATAAAAAAATCTGAATCCGGGAGTAt                                                                                             >  8:62272/1‑151 (MQ=11)
                                                          ccGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTTCTGCTGA‑‑TATTtata                                                                                                                                            >  7:1647082/1‑102 (MQ=255)
                                                          ccGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCAGATCAAGTAAGAGATGGTGAATCCGGGAGGAtt                                                                                            >  4:1561985/1‑150 (MQ=255)
                                                          ccGGGAGGATTCGAACCTCAGACCGATCGGTTCCTAGCCGAGTACTCTATACAGCTGAGCTACGGATGCATCGGG‑AAAGTTATTTTACTGCAGA‑‑TATTGATACCGCTACAAAAAACAAATAAAGAAAGAGAGGGTggcgcggcggggcta                                                                                            >  3:690125/1‑136 (MQ=11)
                                                          ccGGGACGACTCCAACCTCCGCCCGCGCCGCTCGTAGCCGAGTACTCTATCCAGCTGCGCTACGTACGCAGCGGG‑AAATTTAATTTACTGCTGA‑‑TATTCAAACCCCTACTACAGCCATATCAAGTACCAGATGGTGCATCCGGGAGGATTc                                                                                           <  5:1504803/151‑1 (MQ=14)
                                                           cGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCg                                                                                          <  1:956622/151‑1 (MQ=40)
                                                           cGGGAGGATTCGAACCTCCGACC‑CTCGGTTCCTAGCCGACTACTCTATCCAGCTGAGCAACCGATGCAACGGG‑AAATTTAATTTACTGCTGA‑‑TATTTATAACGCTACTAAAACCAAATCAACTAAGAGATGGTGCATCCGGGAGGAtt                                                                                            >  5:881117/1‑148 (MQ=11)
                                                            gggAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTActgct                                                                                                                                                        >  6:86661/1‑90 (MQ=38)
                                                            gggAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTATGAGATGGTGAATCCGGGAGGAttac                                                                                          >  6:1301005/1‑148 (MQ=255)
                                                            gggAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGggagagatcgc                                                                                         >  4:1244582/1‑144 (MQ=255)
                                                              gaggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCAGATCAAGTAAGAGATGGTGCATCCGggaggagtagacc                                                                                       >  6:1400391/1‑144 (MQ=255)
                                                              gaggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTATTTTACTGCTGA‑‑TATTGATAACCCTAATAAAGCCATATAAATTAAAAGATCGTGAAtaagcgagaataataa                                                                                        >  8:1413373/1‑135 (MQ=11)
                                                                ggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCt                                                                                     >  7:94285/1‑151 (MQ=255)
                                                                ggaATCCAACCTCCCACCGCGCCGCGCGGAGCCGCGGACTCCATCCAGCTGATCTACCTCCCCATCGGG‑ACATTTAATTTACTGCACA‑‑TATTCATCACGCTACTACAGCCATATCAAGTAATAGATGGTGCATCCGGGAGGATTCGAACCt                                                                                     <  4:1779862/151‑1 (MQ=9)
                                                                 gaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACAGCTACTAAAGAGATATCAAGTAAGAGATCGTGCATCCGGGAGGATTAGacgcgc                                                                                    >  6:315640/1‑146 (MQ=14)
                                                                 gaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTAATTTACTGCTGA‑‑TATTTATAAAGCTAGTATAGCGATCTCAAGAAAAAGATATTGAATCCCGGAGCAtaatactctc                                                                                    >  5:348226/1‑142 (MQ=11)
                                                                     cGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTATTTTACTGCTGA‑‑TTTTTATACCGCTACTAAAGCCATATCAAGTAAAAGATGGTGAATCCGCGAGGATTAGacgcgacgac                                                                                >  8:1727896/1‑142 (MQ=11)
                                                                       atccTCCCACCGCGCCGCGCCTAGCCGCGCTCTCTATCCAGCTGTGCTACATCTTCATCGGC‑ACATTTTATTTACTGTTGA‑‑TATTCCAACCGATACTACCGCCATATCAAGTACCAGATGGTGCATCCGGGAGGATTCGAACCTCCGa                                                                                 <  5:1299912/146‑1 (MQ=9)
                                                                       aaCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGacgcgagcgcac                                                                              >  7:807607/1‑140 (MQ=255)
                                                                               acggctagtgtcttAGCCGAGGAGTCTATCCAGCTGCGCTACTTAGGCAGCGGG‑ATCTTTATATTCCTGCTCC‑‑TATTGATAACGCTAATACCGGAATATCACGTACCATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTc                                                                     <  4:450870/138‑1 (MQ=12)
                                                                                            gaGCCGAGTACTCTAACCAGCTGAGCTACGGATGCATCGGG‑AAATTTAATTTACTGCTGC‑‑TATTGATAGAGCGACTAAAGCGATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTAct                                                        <  1:542072/150‑1 (MQ=21)
                                                                                                  aGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TAtt                                                                                                                                                <  6:1468771/58‑1 (MQ=255)
                                                                                                  aGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGCGAGGATTCGAACCTCCGAGCGCGAG‑GATCGCCGCCGAGCAcacatccc                                                  >  4:229788/1‑144 (MQ=17)
                                                                                                     actctttactgcaccgCTACGGAGGCATCGGC‑AAAGTTATATTCCGCCTGC‑‑TCTTCATACCGGTCCTATAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTATCCAGc                                               <  4:273113/136‑1 (MQ=17)
                                                                                                       tctATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTTATAACGCTACTAAAGACATATAAAGTAAGAGATGGTGCATCCGGGAGGATTACACGCGCCGACCCCCAC‑GCTCGTAGCCGATTAcaaaaacccgatt                                             >  1:1830485/1‑139 (MQ=14)
                                                                                                        ctATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGAATCAGCGAGGATTAGACGCGCCGAGCGGCGC‑GCTCGTCGCCGAGTAGAAAATCCAGCttt                                            >  7:1104452/1‑149 (MQ=14)
                                                                                                         tATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATAAAGTAAGAGATGGTGCATCCGGGAGGATTAGACGCGCCGACCGGAGC‑GCTCGTCGCCGAGTACTCAAACCAGCttac                                           >  7:492007/1‑148 (MQ=14)
                                                                                                          aTCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGACGCGCCGAGCG‑TCGCGCTCGTCGCCGAGTAGAATAACCAGCtttcc                                          >  6:647295/1‑147 (MQ=14)
                                                                                                            ttcttccgatctcGGTTGCATGGGGAAAAGTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGATCGCTCG‑GTTCGTAGCCGAGTACTCTATCCAGCTGAg                                           <  8:1292313/137‑1 (MQ=18)
                                                                                                            acggctgtgtatcctaTGGATCGGG‑ATATTTTTTTTAGTAGTTC‑‑TCTTGTCACCGCTACTTCAGGCATATCAAGTACCAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTATCCAGCTGAGCTa                                        <  6:754999/136‑1 (MQ=9)
                                                                                                              aGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATAGCGCTAGTAAAGCCATATCAAGTAAGAGATGGTGCATCAGGGAGGATTAGACGCGCCGAGCGGAGC‑GAACGTGGCCGAGTAGTCAAACCAGATCAGCTa                                        >  8:366329/1‑149 (MQ=9)
                                                                                                                  taGCTACGGAGGCAGAGGG‑AAATTTTTATTCCTCTTCA‑‑TATTGCAAACGCTAATTAAGGAATATTTAGTATGATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGAt                                   <  7:1480079/149‑1 (MQ=2)
                                                                                                                        cGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTCGACCCGCCGATCGCGCG‑GTTCGTCGCCGAGTACTCTATCCAGCTGAg                                           >  7:1294432/1‑137 (MQ=14)
                                                                                                                         ggATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAACggg                           >  1:1152277/1‑151 (MQ=255)
                                                                                                                                        acttattcTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTCCAACCTCCGACCg                                                                              >  6:558072/3‑87 (MQ=11)
                                                                                                                                             ttctACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTACCTCCG‑CCGCTAGCGTTGGTAGCCGTGTACTATATCCAGCTAACCTACGTATTCATCGGGCATAATACTTAACTgt           >  1:1259548/4‑147 (MQ=2)
                                                                                                                                              tctACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCg                                                                                  <  5:222591/75‑1 (MQ=255)
                                                                                                                                                 aCTGCTGA‑‑TCATGTCAGCGCTCCTATAGGCATATCTAGTATTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGAtttt    <  7:1060894/151‑1 (MQ=14)
                                                                                                                                                   tgctgA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTACACCCTCAGCCCCTAGG‑GTTGGTCGCCGATGACTCTATCAAGGAGAGCGACGGATCCAACGGGTCACGTCCATTACGCGAGAtttttt  >  7:1017779/1‑151 (MQ=2)
                                                                                                                                                     ctgA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGt                                                           <  2:1178865/92‑1 (MQ=255)
                                                                                                                                                      tgA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGaa                                                                                        <  7:1893350/63‑1 (MQ=40)
                                                                                                                                                      |                                                                                                                                                      
TAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTT  >  NC_000913/2818009‑2818305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑