Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,269,9940GT28.8% 317.9 / 32.2 222intergenic (+392/+631)yhaC/garKpentapetide repeats‑related protein/glycerate kinase I
Reads supporting (aligned to +/- strand):  ref base G (17/114);  new base T (0/53);  total (20/202)
Fisher's exact test for biased strand distribution p-value = 3.61e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 224 total aligned reads displayed.

GAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGT‑GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAGCG‑GGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTAT  >  NC_000913/3269845‑3270140
                                                                                                                                                      |                                                                                                                                                    
gAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGT‑GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTg                                                                                                                                                      <  6:928966/150‑1 (MQ=255)
gAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGT‑GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGa                                                                                                                                                     <  1:338849/151‑1 (MQ=255)
 aaaCCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGT‑GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGaa                                                                                                                                                    <  1:124992/151‑1 (MQ=255)
  aaCCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGT‑GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGaaa                                                                                                                                                   <  1:1831256/151‑1 (MQ=255)
    cccGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGT‑GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACt                                                                                                                                                 <  2:338656/151‑1 (MQ=255)
    cccGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGT‑GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACt                                                                                                                                                 <  5:1281629/151‑1 (MQ=255)
    cccGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGT‑GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACt                                                                                                                                                 <  8:365860/151‑1 (MQ=255)
     ccgccgGAACTGTTTATTACGTAAATCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGT‑GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTg                                                                                                                                                <  7:1515066/151‑1 (MQ=255)
     ccgccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGT‑GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCTAAGCGGGTTTTTACGTAAAACAGGTGAAACTg                                                                                                                                                <  6:1542596/151‑1 (MQ=255)
     ccgccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGT‑GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTg                                                                                                                                                <  6:1265633/151‑1 (MQ=255)
     ccgccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGT‑CGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTCAAACTg                                                                                                                                                <  1:1499254/151‑1 (MQ=255)
      cgccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGT‑GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGa                                                                                                                                               <  3:1235199/151‑1 (MQ=255)
       gccgGAACGGTTTATTATGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGT‑GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAc                                                                                                                                              <  8:1516210/151‑1 (MQ=255)
        ccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGT‑GGCCAGTCATTCATCTTTGTGCCCCACCAAAAGAAAAAACCCCCCGCACCGGGTTTTTAACTAAAACAACTGAAAcacccc                                                                                                                                             >  7:1463833/1‑146 (MQ=17)
        ccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGT‑GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAcc                                                                                                                                             <  4:1557344/151‑1 (MQ=255)
           gAACGGTTTATGACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGT‑GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGAt                                                                                                                                          <  4:1367545/151‑1 (MQ=255)
             aCGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGT‑GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATaa                                                                                                                                        <  6:1865470/151‑1 (MQ=255)
             aCGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGT‑GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATaa                                                                                                                                        <  5:1591321/151‑1 (MQ=255)
             aCGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGT‑GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATaa                                                                                                                                        <  1:1325471/151‑1 (MQ=255)
             aCGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCCAATTCATATCGGTATATTGTCGT‑AGACAGTAAATCAAATTTAAGACCAACCAAAAAAAAAAAACCAAAAAAAAAAAATATTAAGTAAAACAGGTGAAACAGACCCATa                                                                                                                                         >  2:944267/1‑150 (MQ=255)
              cGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGT‑GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAg                                                                                                                                       <  2:634286/151‑1 (MQ=255)
               ggTTTATTACGTACATCAGGTAATACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGT‑GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGc                                                                                                                                      <  5:18679/151‑1 (MQ=255)
               ggTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGT‑GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGc                                                                                                                                      <  4:262744/151‑1 (MQ=255)
                gTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGT‑GGACAGTGATTCATCTTTATGCCCCTCCAAAAGAAAAAAACCCCCGAAGCCGGTTTTTAAGTAAAAAACGTGaaaaggaagcagaagcg                                                                                                                                     >  8:702542/1‑137 (MQ=14)
                 tttATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGT‑GGACAGTCATTCATCTTTCTGCCCATAAAAAAATAAAAACCAGAAGAAAAAGATTTTTACGGAAAAGATATAAAACGGACCGATAAgga                                                                                                                                     >  5:728941/1‑148 (MQ=14)
                   tattaCGTACATCAGGTAAAACTGACCGATAAACCTCTTAATATAGGCTATAATGTAAA‑GCAAATTCATAAATCTTTATAACACTCAAAAAGAAAAAACAATAAAAAAAAAGTTTTTAAGTAAATCAGGTGAAACTGACCGATaatgatcg                                                                                                                                  >  2:447242/1‑145 (MQ=255)
                    attaCGATCATCACCGAAAACTGACCGATTAGCCGCTTTCTTTTGTCTATAGTGTCGT‑CGACAGGCATTTATCTTTCTCCCACTCCAAAAGTAAAAACCCGCCGAAGCGGTTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGctt                                                                                                                                 <  1:1683482/151‑1 (MQ=11)
                      taCTTACATCAGTTATAACTGTGCGATAAGCCGGTTTCTTTTGTGTATAGTGTCGT‑GGACAGTCTTTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGcttt                                                                                                                                <  8:346265/150‑1 (MQ=14)
                      taCGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGT‑GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGcttt                                                                                                                                <  4:593555/150‑1 (MQ=40)
                      taCGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGT‑GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGcttt                                                                                                                                <  8:238786/150‑1 (MQ=40)
                      taCGTAATTAAGGTAAAAGTCTCCGTTATTCCCCTTTGTTTTTTGTATATTGTCTT‑CTTAAGTCATTGATGTTTCTGCCCCTCCAAAATTATAAACCCGCCGAACCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGcttt                                                                                                                                <  1:489789/150‑1 (MQ=2)
                       aCGTACATCAGTTAAAACTTACCGATAAGCGGCTTTCTTTTGGGTATAGTGTCGT‑GGACAGTGATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGctttc                                                                                                                               <  7:1347367/150‑1 (MQ=255)
                       aCGTACATCAGGTAAATCTGTCCGATAAGCCGCTTTCTTTTGGTTTTAGTGTCGT‑GGACAGTGATTCATCTTTATGCCCCTCCAAAATTATAAACCCGCCGTAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGctttct                                                                                                                              <  5:1661663/151‑1 (MQ=14)
                       aCGTACATCAGGTAAAACCGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGT‑GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGctttc                                                                                                                               <  6:1801777/150‑1 (MQ=255)
                        cGTACCTCACGTAAAACTGACCGATAAGCCGCTTTCTTTTGTGTATAGTGTCGC‑GGACAGTCATTCATCTATCTGCCACTCCAAAAGTAAAAACCCGCCGCAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGctttct                                                                                                                              <  2:271745/150‑1 (MQ=14)
                        cGTACATAACTTAATAATTACCGATAATCAGATTTCTTTTGCCTATCGTGTCGT‑CGACAGTCTTTCCTCTAAATCCCACTCAAAAATTAAAAACCCGCCGCACCGGGTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGctttctt                                                                                                                             <  2:1408876/151‑1 (MQ=2)
                         gTACATCAGGTAAAACTGACCGATAAGCCGCTGTCTATTGGGTATAGTCTCGTCGCACAG‑AATTCTACTTTGGGGCACACCAAAACTAAAAAAAAAAACAAGCGGGGTTTTACGTATATCCGGTGCAACTGACCgcgaaagcgcgggcg                                                                                                                              >  1:927704/1‑135 (MQ=9)
                             atcatttAAAACTTACCGATAAGCGGCTTTCTTTTGTCTCTAGTGTCGG‑GGACAGTCATTCATCTTTCTGCCCCTCAAAAATTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTggg                                                                                                                        <  6:392508/145‑1 (MQ=11)
                             atcacttAAAACTGACCGATAATCCGCTTTCTTTTGGCTTTCGTGTCGT‑GGACTGTCATTCCTCTTTCTGGCACTCCAAAAGTACAAACCCGCCGCACCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTggg                                                                                                                        <  3:422345/145‑1 (MQ=11)
                              acAGGTAAAACTGACCGATTATATTATTTCTTTTGGTTATAGTGTAGT‑TTACATTCATTCATCTTTCTTCCCCTCCAATAGGAAATTCCGTTCTTATCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGt                                                                                                                       <  8:1186210/150‑1 (MQ=2)
                               aaGGTAAAACTGACCGATAAGCGGCTTTCTTTTGGGTATAGTGTCGT‑GGACAGTCATTCATCTTTCTGCCACTCCAAAAGTAAAAACCCGCCGAATCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGta                                                                                                                      <  5:61710/150‑1 (MQ=255)
                                   tAACACTGACCGATAAGCCGCTTTCTTTTTGGTATAGTGTCGT‑GGACAGTGTTTCATCTATCTGCCCCTCCAAAAGTAAAAACCCGCCGAATCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAg                                                                                                                   <  3:258436/150‑1 (MQ=11)
                                     aaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGT‑GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTATGGGTATAgtgt                                                                                                                <  4:830892/151‑1 (MQ=25)
                                      aaCTGACCGATCAGCCGCATTCTTTTGCGTATAGTCTCGT‑GGACAGTCATTCAACTTTCTGCCACTCCAAAATTAAAAACCCGCCGCAGCGGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTc                                                                                                               <  4:884572/151‑1 (MQ=11)
                                          gACCGATCAGCAGCTTTCTTTTGGGTCTCGTGTCGT‑GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTgg                                                                                                           <  7:1422639/151‑1 (MQ=255)
                                          gACCGATAATCCGCTTTCTTTTGCCTCTAGTCTCGA‑GGACAGTCATTCATCTATCTGCCCCTCCAAAAGTAAAAACCCGCCGAACCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTg                                                                                                            <  4:1023317/150‑1 (MQ=11)
                                          gACCGATAAGCCGCTTTCTTTAGCGTAGCGTGTCGT‑CGACAGTCATTCATCTTTCTGCCCCTCAAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGt                                                                                                             <  1:709394/149‑1 (MQ=11)
                                            gcgttcatctgtattctttTGCTTTTATTGTCGT‑GGCCAGTCTTTTATATTAATGCCACTCAAAAATTATAAACCCGCCGCATCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAc                                                                                                         <  2:1226624/138‑1 (MQ=2)
                                            ccGATCAGCGGCATTCTTTTGCGGACCGTGTCGT‑CGACACGGTCTCAACTATCTCCCACTCCAAAATCACAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAc                                                                                                         <  4:709194/151‑1 (MQ=2)
                                            ccGATAAGCAGCATTCATTAGCTTAGAGTGACGT‑GGAAAGGCAATCATATATATGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAc                                                                                                         <  6:795787/151‑1 (MQ=11)
                                              gatatgttgCTTTCTTTTTTCTATTGTGTCGG‑CGACAGTGTTTTTTCTTTATCGCACTCCAAAAGTTCAAACCCGCCGCATCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAg                                                                                                       <  3:746526/143‑1 (MQ=2)
                                                tAAGCCGCATTCTTTAGGCTCTTGTGTCGT‑GGAAAGTCATTCTTATTTCTGCCCCTCAAAAAGTATAAACCCGCCGAATCGGTTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGt                                                                                                      <  8:1744490/150‑1 (MQ=11)
                                                 aaGCTGCTTTCTTTTTGGTTTAGTGTCGT‑GGACTGTGTTACATCTTTATGCCACTCCAAAAGTTCAAACCCGCCGTAGCGGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGtca                                                                                                    <  3:1864058/151‑1 (MQ=11)
                                                    gggCTTTCTTTTGTGTATGGTGTCGT‑CGACTGTCATTGATCTTTCTGCCACTCCAAAATTAAAAACCCGCCGAAGCGGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGtcattc                                                                                                 <  7:368077/149‑1 (MQ=11)
                                                                  attttgtgtCGT‑GGAATGTGTTTATTATTTCTGGCACTCAAAAAGTTTAAACCCGCCGTATCGGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGcccc                                                                                    <  8:1052175/145‑1 (MQ=11)
                                                                   tataGTGTCGT‑CGAAAGGCATTCATCTATCTGCCCCTCCAAAAGGAAAAACCCGCCGCACCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCt                                                                                   <  7:1890930/150‑1 (MQ=11)
                                                                        tgtCGT‑GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAATCCGCTTTCGTTTGGGGATAGTGTCGTGGCCAGTAATTGATCTTTTTTGCCCTCCaaa                                                                              >  7:638449/1‑150 (MQ=11)
                                                                         gtCGT‑GGACAGTGATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGCAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAg                                                                            <  7:108716/151‑1 (MQ=25)
                                                                         gacGT‑CGACAGTCCTTAATCTTACTGCCACTCAAAAATAAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaaa                                                                             <  5:1634258/148‑1 (MQ=11)
                                                                          cacggggacacactatcatCTATCTGCCACTCCAAAAGGACAAACCCGCCGCAGCGGGTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaaa                                                                             <  4:530363/135‑1 (MQ=11)
                                                                                gcctgcgcgatgacTTACTGCCCCTCAAAAAGCTAAAACCCGCCGTACCGGGTTATTACGTACAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaaaaa                                                                      <  1:1078934/138‑1 (MQ=11)
                                                                                    gtgttagctatttCTGGCACTCAAAAAGTTAAAACCCGCCGTAGCGGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccg                                                                  <  8:47135/141‑1 (MQ=11)
                                                                                       gtaccaataacTGCCACTCCAAAAGGACAAACCCGCCGAACCGGGTTTTTACGTAAAACAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgccg                                                               <  1:1847017/141‑1 (MQ=11)
                                                                                        cgcacctcccagCCCCTCCAAAACCAAAAACCCGCCGAAGCGGGTTTTTATTTAAAAAATGTTAATCTGACCTATAAGACGATTTCTTTTGGGTATAGTGTTGTGGACAGTGATTCTTTTTTCTGCTCCTCCAAAAGCAAAAACCCGCCG‑a                                                             <  8:949895/140‑1 (MQ=2)
                                                                                          catCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATaa                                                                                                                                        >  1:304967/1‑75 (MQ=255)
                                                                                            aatttatcccacTCCAAAATAAAAAACCCGCCGCACCGGTTTATTACGTAAATCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAGCg                                                         <  5:1828490/140‑1 (MQ=11)
                                                                                               aaatcgcacTCAAAAATTAAAAACCCGCCGAACCGGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAGCG‑GGt                                                     <  3:586412/143‑1 (MQ=11)
                                                                                                tctcCCACTCAAAAATGACAAACCCGCCGTAGCGGTTTTTTACGTACAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGGCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAGCG‑GGt                                                     <  4:1067307/150‑1 (MQ=11)
                                                                                                actcccacTCCACAAGCACAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAGCG‑GGtt                                                    <  6:113231/144‑1 (MQ=11)
                                                                                                   cccacTCCACAAGCAAAAACCCGCCGCAGCGGGTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAGCG‑GGtttt                                                  <  7:816310/146‑1 (MQ=11)
                                                                                                     cccTCCAAAAGGACAAACCCGCCGCAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAGCG‑GGTTTTTAc                                               <  7:128634/151‑1 (MQ=16)
                                                                                                      ccTCCAATATTATAATCTCTCCGTATCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAGCG‑GGTTTTTACg                                              <  8:1461451/151‑1 (MQ=11)
                                                                                                        gccACAAGCACAAACCCGCCGCAGCGGGTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAGCG‑GGTTTTTACGTa                                            <  6:505494/150‑1 (MQ=255)
                                                                                                         cctaaaTTATAAACCCGCCGTAACGGTTTTTTACGTAAAACAGGTTAAACTGACCGATAAGGCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAGCG‑GGTTTTTACGTa                                            <  8:1549842/147‑1 (MQ=11)
                                                                                                         caaaaatcataaaCCCGCCGCATCGGTTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAGCG‑GGTTTTTACGTaa                                           <  3:1155397/141‑1 (MQ=11)
                                                                                                          aaaaaGTATAAACCCGCCGTATCGGGTTTTTACGTACAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAGCG‑GGTTTTTACGTaaa                                          <  3:1673954/150‑1 (MQ=255)
                                                                                                           acaaGTAGAAACCCGCCGCAGCGGTTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAGCG‑GGTTTTTACGTAAAc                                         <  3:505700/149‑1 (MQ=255)
                                                                                                             aagattaaaCCCGCCGTATCGGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAGCG‑GGTTTTTACGTAAACCa                                       <  2:1450605/145‑1 (MQ=11)
                                                                                                                tAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGTTGATACTGACCGATAAGCCGCTTTCTTTTGTTTATAGAGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAGCG‑GGTTTTTACGTAAATCAg                                      <  5:1688140/149‑1 (MQ=11)
                                                                                                                tAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGAGAAGGCGCTTTCTTTTGGGGATAGTGTCGTGGCCGGTCAGTGCTGGTTCTGGCCCTCCAAAAGCAAAAACCCGCCG‑CGGCG‑GGGTTTTTCggatagccgcg                                    >  6:1688670/1‑141 (MQ=11)
                                                                                                                 tcaaaCCCGCCGAAGCGGTTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAGCG‑GGTTTTTACGTAAACCAGGt                                    <  3:923366/148‑1 (MQ=255)
                                                                                                                 acaaccccGCCGCAACGGTTTATTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGGCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAGCG‑GGTTTTTACGTAAACCAGGTg                                   <  7:457903/146‑1 (MQ=11)
                                                                                                                 acaaaCCCGCCGAATCGGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAGCG‑GGTTTTTACGTAAACCAGGt                                    <  5:381327/148‑1 (MQ=255)
                                                                                                                 aaaaaCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAGCG‑GGTTTTTACGTAAACCAGGTg                                   <  7:1102203/151‑1 (MQ=35)
                                                                                                                           ggtcttccgTTTTTACGTAAATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAGCG‑GGTTTTTACGTAAACCAGGTGAAACTGACCg                         <  5:99238/143‑1 (MQ=255)
                                                                                                                            gAAGCGGGTTTTTACGTAAAAAAGGTGAAACTGACCTATAATCCTCTTTGTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAGCG‑GGTTTTTACGTAAACCAGGTGAAACTGACCGa                        <  8:637405/151‑1 (MQ=255)
                                                                                                                              agcgttttattACGTACAACATTTTAAACTGACCGATAAGCCGCTTTCTTTTGCGTATAGTGTCGTGGAAAGTCTTTCATCTTTCTGCCACTCAACAAGCAAAAACCCGCCG‑AAGCG‑GGTTTTTACGTAAACCAGGTGAAACTGACCGATa                      <  3:1326110/142‑1 (MQ=11)
                                                                                                                              agaggttttttACGTAAAACATTTTTAACTTACCGATAAGTTGCTTTCTTTTGTGTATAGTGTCGTGGAATGTCATTCCTCTTTCTGCCCCTCAAAAAGCAAAAACCCGCCG‑AATCG‑GGTTTTTACGTAAACCAGGTTAAACTGACCGAt                       <  5:1452821/144‑1 (MQ=11)
                                                                                                                              acagttttattACGTACAAAATTTTAAACTGACCGATAAGCTGCTTTCTTTTTTGTTTAGTGTCGGGGAAAGTCAATCATCTTTCTGCCCCTCAAAAATCAAAAACCCGCCG‑AAGCG‑GGTTTTTACGTAAACCAGGTTAAACTGACCGATa                      <  3:297960/142‑1 (MQ=11)
                                                                                                                               cgggttttttACGTAAAACAGGTTTTACTGACCGATAAGATGTTTTCTTTTGTGTTTCGTGTCGTGGACAGGGATTCATCTATCTGGCCCTCCAAAAGAATAAACCCGCCG‑AAGCG‑GGTTTTTACGTAAACCAGGTGAAACTGACCGATa                      <  3:289465/145‑1 (MQ=11)
                                                                                                                                cgttttattACTTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGTGTTTTGTGTCGTGGAATGTCATTCATCTTTCTGCCACTCCAAAATCAAAAACCCGCCG‑AACCG‑GGTTTTTACGTAAACCAGGTGAAACTGACCGATaa                     <  2:1347336/143‑1 (MQ=11)
                                                                                                                                cGGTTTTTTACGTAAATCATTTTAAACTGACCGATAAGCCTCTTTCTTTTGGGTATATTGTCGTCGACATTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAGCG‑GGTTTTTACGTAAACCAGGTGAAACTGACCGATAAg                    <  4:806568/151‑1 (MQ=11)
                                                                                                                                  tgTTTTTACGTACCTCAGGTGAAAGTGACCGATCAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAGCG‑GGTTTTTACGTAAACCAGGTGAAACTGACCGATa                      <  4:1629847/146‑1 (MQ=11)
                                                                                                                                   gattttACGTACCACATGTGAAACTGACCGATAAGCCGGTTTCTTTTGGGTATAGTGTCGTGGAAAGTCATTCTTCTTTCTGCCCCTCCAAAAGCATAAACCCGCCG‑AAGCG‑GTTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCg                 <  1:618162/149‑1 (MQ=11)
                                                                                                                                      attACGTAAAACAGGTAAATCTGACCTCTAACGCGGTTTCTTTTTGGGATAGTGTCGCGGCCAGGCACTCCTCTTTATCCCCCTCCAAAAGCAAAAACCCGCCG‑CAGCG‑GGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctt              <  4:1462544/150‑1 (MQ=11)
                                                                                                                                        tACGTAAAAGAGGTGATACTTACCGATAATTAGCTTGCTTTTGGCGATAGTGTCGTCGCCAGTGTTTCTTCTATCTGCCCCTCCAAAATCAAAAACCCGCCG‑AAGCG‑GGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGcttt             <  7:959281/150‑1 (MQ=14)
                                                                                                                                         aCGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTATAGTGTCGTGGCGAGTCCTTCATAGTTGGCCCCCGGCAAAACCAAAAACCCGCCG‑CAGCGCGGTTGTTA‑GTAAACCACGTGCAAGTGCCCCATCATCCgggttgt           >  4:1753862/1‑145 (MQ=2)
                                                                                                                                             acaaCAGCTCAAACAGACCGATACGCCTCTTTGTTTAAGGAATAGAGTCGTTCAGAGTCATTCTTATTTGAGTCGATACAACACGTCATACCCTCCG‑AAGCG‑GGTTTTTACGTAAACCAGGTCAAACTGACCGATAAGCCGctttcttt         <  6:458461/147‑1 (MQ=2)
                                                                                                                                             acaaCACGTTAAACTGACCGATAAGCCGCTTTCTTTTGCCTCTAGTGTCGTCGACAGTCATTCCTCTTTCTGCCCCTCCACAAGCAAAAACCCGCCG‑AACCG‑GGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCtttt        <  7:849341/148‑1 (MQ=11)
                                                                                                                                                  aGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGTACAGTCATTCAAGGTTGTGCACCAGCAAAAACAAAAACCCGCAGCAGGGG‑GGGTTTTAC‑TATATGAAGTGAAAAAGACCGATAATCCGCGTTCATTTGTGtat  >  4:860159/1‑151 (MQ=2)
                                                                                                                                                      |                                                                                                                                                    
GAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGT‑GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAGCG‑GGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTAT  >  NC_000913/3269845‑3270140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑