Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,392,4570GT26.2% 379.8 / 24.4 245intergenic (+22/‑15)glyV/glyXtRNA‑Gly/tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base G (61/97);  new base T (2/54);  total (67/178)
Fisher's exact test for biased strand distribution p-value = 1.03e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 250 total aligned reads displayed.

CCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑ATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAG  >  NC_000913/4392309‑4392604
                                                                                                                                                    |                                                                                                                                                     
cccGTAACGACGCAGAAATGCGAAAATTACGATAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGc                                                                                                                                                      <  6:135527/150‑1 (MQ=255)
cccGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAg                                                                                                                                                       <  7:238945/149‑1 (MQ=255)
cccGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAg                                                                                                                                                       <  5:1211828/149‑1 (MQ=255)
 ccGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGcac                                                                                                                                                    <  8:1096276/151‑1 (MQ=255)
  cGTAACGACGCAGAAATGCGAAAATTACGAAAGGAAAATTAAGTAGTACGCGGGAATAGGTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTGGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGcaca                                                                                                                                                   <  1:605791/151‑1 (MQ=255)
   gTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAg                                                                                                                                                  <  2:328680/151‑1 (MQ=255)
     aaCGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑ac                                                                                                                                               <  4:760750/151‑1 (MQ=255)
     aaCGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑a                                                                                                                                                <  3:1292896/150‑1 (MQ=255)
      acgacgCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑acc                                                                                                                                              <  3:1224078/151‑1 (MQ=255)
        gacgCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑accac                                                                                                                                            >  4:1721520/1‑151 (MQ=255)
          cgCAGAAATGCTAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCTCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑ACCAccc                                                                                                                                          <  4:815026/151‑1 (MQ=255)
          cgCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑ACCAccc                                                                                                                                          <  4:546882/151‑1 (MQ=255)
           gCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGTCGTCGCCTCTCGGGTCCCCCGCCAAAAAAGGAAAATGGCTGCCGTGCGCCG‑ACCAccc                                                                                                                                          >  6:1539813/1‑150 (MQ=18)
           gCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑ACCACCCa                                                                                                                                         <  4:127712/151‑1 (MQ=255)
            cAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑ACCACCCaa                                                                                                                                        <  7:642854/151‑1 (MQ=255)
             aGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑ACCACCCAAg                                                                                                                                       <  2:1527737/151‑1 (MQ=255)
              gAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑ACCACCCAAGc                                                                                                                                      <  3:522429/151‑1 (MQ=255)
                   gCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑ACCACCCAAGCGGGaa                                                                                                                                 <  6:797669/151‑1 (MQ=255)
                   gCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTAAAAAAATAGAAAAGTGGTGCAAAACACTG‑acccacaatggggaa                                                                                                                                  >  8:1515130/1‑138 (MQ=18)
                    cGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑ACCACCCAAGCGGGAAt                                                                                                                                <  1:1008736/151‑1 (MQ=255)
                    cGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTATCCCGCGCCAAAAGTTTAAACGGGCGGCCACGCACAG‑ACCACCCCCGCGGGacg                                                                                                                                >  6:422420/1‑149 (MQ=18)
                     gAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCATAATTTGAAAAGTGCTGCAAAGCACAG‑ACCACCCAAGCGGGAATa                                                                                                                               <  3:195881/151‑1 (MQ=255)
                     gAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑ACCACCCAAGCGGGAATa                                                                                                                               <  1:325514/151‑1 (MQ=255)
                     gAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑ACCACCCAAGCGGGAATa                                                                                                                               <  8:1295950/151‑1 (MQ=255)
                      aaaaTTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑ACCACCCAAGCGGGAATAg                                                                                                                              <  2:1302545/151‑1 (MQ=255)
                      aaaaTTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑ACCACCCAAGCGGGAATAg                                                                                                                              <  7:357380/151‑1 (MQ=255)
                       aaaTTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGACAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAATTCATGGAAAGCAACG‑CCAACCCaatgtggtatagc                                                                                                                             >  1:1850261/1‑140 (MQ=17)
                           tACTAAAGATAAATTAACTCGTACGCGGGAATATCTCAGTTTGTAGTGCACGACATTGCCAAGGTCGGGGTCGCTATTTCGAGTCTCGTTTCCCGCTCCAAAATTT‑TAAAGTGCTGTAAGGCACAG‑ACCACCCAAGCGGGAATAGCTCAg                                                                                                                         <  3:1696514/150‑1 (MQ=11)
                           tACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑ACCACCCAAGCGGGAATAGCTCa                                                                                                                          <  3:1619768/150‑1 (MQ=255)
                            aCGAAAGGAATATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGAGCTTGCCAAGGTCGGTGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAATGTGCTGCAAAGCACAG‑ACCACCCAAGCGGTAATAGCTCAg                                                                                                                         <  2:227550/150‑1 (MQ=17)
                             cGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑ACCACCCAAGCGGGAATAGCTCAGtt                                                                                                                       <  5:408443/151‑1 (MQ=255)
                              gAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑ACCACCCAAGCGGGAATAGCTCAGTTg                                                                                                                      <  8:1544785/151‑1 (MQ=255)
                                   cAAAATTTAGTAGTACGCGGGAATAGCTCAGTTGTTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTACCGCTCAAAAATTTGAAAAGTGCTGCAAATCACAG‑ACCAACCAAGCGGGAATAGCTCAGTTGGTag                                                                                                                  <  8:1794437/150‑1 (MQ=17)
                                   cAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTTAAAACTGATGAAAATCACAG‑CCCACCCAAGCCGGAATAGCTCCGTTGGttgc                                                                                                                 >  4:312098/1‑148 (MQ=17)
                                   cAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTCCAAAGCACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTaga                                                                                                                 <  8:1877209/151‑1 (MQ=255)
                                   cAAAATTAAGTAGTACGCGCGAATAGCTCAGTTGGTAGAGCACGACCTTCCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTCCAAAGCACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTag                                                                                                                  <  4:1511973/150‑1 (MQ=255)
                                    aaaaTTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAA‑TTCATTAAAGCACAGCACAACGCAAGAGCGAATAGCACAGtaagaatat                                                                                                                >  5:687305/1‑143 (MQ=14)
                                     aaaTTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑ACCACCCAAGCGGGAATAGCTCa                                                                                                                          >  3:104654/1‑140 (MQ=255)
                                       aTTATGTACTACGCGGGAATAGCTCAGTTTGTAGAGGACGTCCTTTCCTAGGGCGTGGTCGCGAGTTCGCGTCTCCTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAATCACAT‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCAc                                                                                                             <  5:43908/151‑1 (MQ=14)
                                       aTTAAGTAGTAACCGGGAATACCTCAGTTGGTAGAGCACGACCTTGCCAATCGCGTGGTCGCTATTTGGCGCCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAATCACAT‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCAc                                                                                                             <  1:1527192/151‑1 (MQ=14)
                                         tAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCAAAAATTTGAAAAGTGCTGCAAATCACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACg                                                                                                            <  6:735264/150‑1 (MQ=255)
                                          aaGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATATGAAAACTAATAAAAATCCCAA‑ACCACCCAATCCGGAATAGCTCAttttttatatcacta                                                                                                           >  3:315304/1‑138 (MQ=14)
                                               gtaCGCGGGAATAGCTCAGTTAGTTGAGCACCACCTAGAGAAGGTCAGGGTAGCGAGTTCTAGTCTAGATTACCGGTCCAAAATTTTAAAATAAAATAAAAGCACAG‑ACCCCCCAAGGGGGAATAGGTCAGTTGGGAGGGcgcggcggggg                                                                                                     >  5:1527465/1‑144 (MQ=255)
                                                 aagcgGGAATAACTCAGTTTGTTGAGGACGAGCTTTGCAAGCTCTTGGTCGCGAGTTCGAGGCTCCTTTCCCTCTCCTAAATTTGAAAATTCCTCCAAACCAAAT‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCa                                                                                                   <  1:1060221/149‑1 (MQ=2)
                                                      ggAAAAGCTCAGTTGGTAGAGCACGACCTTGCCAACGTCGGGGTCGCGAGTTCGAGGCTCCTTTCCCTCTCCAAAATTTGAAAATTGCTGCAAAGCACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTc                                                                                              <  6:60182/151‑1 (MQ=14)
                                                        aaTATCTAAGTTTTTTGAGGACGTCCTTGCGAAGCTATTTGTCGCTATTTCGAGGCTCGGTTCCCTCTCAAAAATTTGAAAATTGCTTAAAATAACAT‑ACCAACCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCg                                                                                             <  5:1028722/150‑1 (MQ=2)
                                                        aaTAGCTCAGTTGGTAGAGCACGACCTTGCCTATGTCGTGGTCGCTAGTTCGTGTCTCGTTTCCCTCTCCAAAATTTGAAAAGTTCTTCAAATCACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCgg                                                                                            <  2:594825/151‑1 (MQ=14)
                                                         aTACCTCAGTTGGTAGAGCTCGACCTTGCGTAGGTCGGGGTCGCTAGTTCGAGTCGCGTTTCCCTCTCCAAAATTTGAAAAGTGCTGCAAATCACAT‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCggg                                                                                           <  5:1695912/151‑1 (MQ=14)
                                                           aGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCgggg                                                                                          <  7:59981/150‑1 (MQ=38)
                                                            ccTCAGTTTGTAGAGCACGACCTTTCCAAGGGCGGGGTCGCGAGTTCGAGTCTCGTTACCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTc                                                                                        <  3:370957/150‑1 (MQ=255)
                                                             atCAGTTTTTAGAGCAGGAGCTTCCCAATGGCTGTGTCGCTATATCGAGTCTCGTTTCCCGCTCAAAAATTTGAAAAGTGCTTCAAATAACAT‑AACTCCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTc                                                                                        <  5:1275273/149‑1 (MQ=9)
                                                             atCAGTTGTTAGCGGACGACCTTGCGCAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCTCTCCAAAATTTGAAAATTCCTCCAAATCACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTcg                                                                                       <  5:958774/150‑1 (MQ=14)
                                                               cAGTTGGTAGCGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGGCGCGTTTCCCCCTCCAAAATTTGAAAAGTCCTTCAAACCACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTcg                                                                                       <  6:184047/149‑1 (MQ=14)
                                                                    tttatagCATGACCTTCCCAATTTTTTGGTCTCGAGTTCGAGTGTCGTTAACCTCTCAAAAATTTGAAAAGTGCTTCAAACAAAAG‑ACCAACCAAGCGGGAATAGCTCATTTGGTAGATCACGACCTTGCCAAGGTCGGGGTCGCGAGtt                                                                                 <  7:1046724/145‑1 (MQ=9)
                                                                    ggTAGAGGACGACCTTGCCTAGGTCGGGGTCGCGAGTTCGAGGCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTc                                                                                <  2:1000047/151‑1 (MQ=255)
                                                                       agagCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGa                                                                              <  6:1428615/150‑1 (MQ=38)
                                                                        gagCACGACCTTGCCAAGGTCGGGGTCGCGAGTTGGAGTCTCGTTTGCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑ACCACCCCAGCGGGAATAGCGCAGTTGGTAGAGCACGACCTTGCCAAGGTGGGGGGcg                                                                                       >  8:1034899/1‑140 (MQ=14)
                                                                            aCGACCTTGCCAAGTTCGTGGTCGATAGTTCGAGTCTCGTTACCCGCTCAAAAATTAGAAAAGTCCTCAAAATAAAAT‑AACACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCg                                                                        <  4:1050690/151‑1 (MQ=11)
                                                                            aCGACCTTGCCAAGGTCTGGGTCGCGAGTTCGAGGCGCGGTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAACCACAG‑ACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCg                                                                        <  4:1302114/151‑1 (MQ=14)
                                                                                   tgggaatggctgggTCGCGAGTTGGTGTCGCGTTTACCTCTCCTAAATTTGAAAAGTTCGTCATATAACAT‑AACTCCTAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTcc                                                                   <  5:797403/138‑1 (MQ=9)
                                                                                    gCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAT‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCg                                                                 <  1:114839/150‑1 (MQ=38)
                                                                                    gCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCg                                                                 <  7:141064/150‑1 (MQ=38)
                                                                                      gaaGGGCGGGGTCGCGTGTTCGAGGCTCGTTTCCCGCTCCAAAATTTGAAAATTGCTCCAACTCACAG‑ACGAAGCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTc                                                              <  7:1715069/150‑1 (MQ=11)
                                                                                            cGGGGTCGCGAGTTCGAGTCTCGTTACCCTCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCaaa‑a                                                        <  6:269846/150‑1 (MQ=255)
                                                                                              gtgTCGCGATTTCGCGACTCGTTTCCGGCTCCTAAATTTGAAAAGTGCGCCACCGCACAG‑ACCTCCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑Attt                                                     <  7:795607/149‑1 (MQ=14)
                                                                                                gTCGCGACTTCGAGGCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTTCACACCACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑ATTTGa                                                   <  6:1219501/151‑1 (MQ=255)
                                                                                                  cgttatttCGTGTTTCGTTTACTTCTCATATATTTGAAAATTCCTTCATATAACAG‑ACGTCGGAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑ATTTGaaa                                                 <  2:1205488/145‑1 (MQ=2)
                                                                                                  cgcgAGTTCGAGTCGCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑ACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑ATTTGaa                                                  <  7:1182093/150‑1 (MQ=255)
                                                                                                  cgataGTTCGAGTCTCGGTTCCCGCTCCAAAATTTGAAAAGTGCTTCAAAGCACAG‑ACGACGCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑ATTTGaaa                                                 <  8:822144/147‑1 (MQ=14)
                                                                                                   gcgAGTTCGAGGCGCGTTTCCCGCTCCAAAATTTGAAAATTCCTCCACACCACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑ATTTGAAAg                                                <  4:1360473/151‑1 (MQ=14)
                                                                                                    cgatatCGAGGCGCGTTTCCCGCTCCAAAATTTGAAAATTTCTGCACAGCACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑ATTTGAAAGt                                               <  6:1040921/146‑1 (MQ=14)
                                                                                                      tgTTCGAGTCTCGGTTCCCGCTCATAAATTTGAAAAGTCCTTCATATAACAG‑ACCACGCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑ATTTGAAAGTg                                              <  3:622975/149‑1 (MQ=17)
                                                                                                      aGTTGGAGGATCGTTTCCCTCTCCAAAATTAGAAAAGTTCTTCAAATCACTG‑ACCAAGCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑ATTTGAAAGTg                                              <  5:565089/150‑1 (MQ=17)
                                                                                                        ggcGAGGCCCGGTTCCCTCTCCAAAATTCGAAAAGTGCGCCACACAACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑ATTTGAAAGTGCTg                                           <  4:44284/149‑1 (MQ=17)
                                                                                                             ggctcGTTTCCCGCTCCAAAATTGGAAAAGTCCTGCAACTCACAG‑ACCACCCAAGCGGNAATAGCNCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑ATTTGAAAGTGCTGTAAgg                                      <  3:1538540/149‑1 (MQ=255)
                                                                                                              ggtcctttACCGCTCCAAAATTAGAAAATTCCTGAAAAAAAAAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑ATTTGAAAGTGCTGTAAGGc                                     <  6:1111941/146‑1 (MQ=17)
                                                                                                               cgcGTTTCCCGCTCAAAAATTTGAAAAGTCCGCCACATAACAT‑ACGACGCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑ATTTGAAAGTGCTGTAAGGca                                    <  6:1642952/149‑1 (MQ=17)
                                                                                                                tcgtttacgtcgcctaaaTTTGAAAAGTCCTTCAAATAACAT‑ACGACGGAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑ATTTGAAAGTGCTGTAAGGca                                    <  3:1644613/135‑1 (MQ=17)
                                                                                                                 cGTTTCCCGCTCAAAAATGAGAAAAGTCCTGAAAATCACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑ATTTGAAAGTGCTGTAAGGca                                    <  6:672345/149‑1 (MQ=17)
                                                                                                                  gTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGCGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTGGAGTCTCGTTTCCCGCGGCAAA‑TTGTGCGAGGGCTGTAAgggcccg                                 >  4:910442/1‑146 (MQ=14)
                                                                                                                   tttCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCAAAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTAGCGATTACCGCGCCAAATATTTCaaaaactcaaattacgcg                                  >  7:162811/1‑135 (MQ=11)
                                                                                                                    ttCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑ACCACCCAAGCGGGAATACCACAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGCGTCTCGTTTCCCGCTCCAAA‑ATATGAAAGAGCTGAAAGGCacacac                               >  2:861955/1‑151 (MQ=17)
                                                                                                                     tCCCGCGCCAAAATTAGAAAAGTGCTGCAAAGCACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑ATTTGAAAGTGCTGTAAGGCACAGac                               <  6:978960/150‑1 (MQ=255)
                                                                                                                      ccgcctcataaaTTTGAAAATTGCTGCACATCACTG‑AACACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑ATTTGAAAGTGCTGTAAGGCACAGacc                              <  4:1156005/141‑1 (MQ=17)
                                                                                                                      cccGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑ACCACCCAGGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑ATGTGCAAGGGCTGTAAGTCCCACaccc                             >  6:476894/1‑150 (MQ=14)
                                                                                                                      cccGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑ATTTGAAAGTGCTGTAAGGCACAGacc                              <  6:628562/150‑1 (MQ=255)
                                                                                                                      cccGCTCCAAAATTTGAAAAGTGCTCCAAACAACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑ATTTGAAAGTGCTGTAAGGCACAGacca                             <  1:919299/151‑1 (MQ=255)
                                                                                                                        cGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑TTTTGCAAGTGCTGTAATGgacacagcccc                           >  1:1553352/1‑144 (MQ=14)
                                                                                                                           tCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑ATTTGAAAGTGCTGTAAGGCACATACCAccccc                        >  7:974077/1‑149 (MQ=255)
                                                                                                                            ccAAAATTTGAAAAGTGCTGCAAAGCACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑ATATTAAAGATATGTAATGAACAGACCACCCa                         >  2:1666657/1‑149 (MQ=17)
                                                                                                                            cataaaTTAGAAAATTGCTCAATATCAAAG‑ACTTCGCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑ATTTGAAAGTGCTGTAAGGCACAGACCACCCa                         <  8:890329/146‑1 (MQ=14)
                                                                                                                             cAAAATTTGAAAAGTGCTGCAAAGCACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑ATATTAAAGAAATGTAATGAACACACCGcccccgc                      >  5:1415986/1‑147 (MQ=14)
                                                                                                                                aaTTTGAAAAGTGCTGCAAAGCACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑ATGTGATAGTGCTGTAAGAAACACAccccccccccgcg                   >  1:1304398/1‑144 (MQ=17)
                                                                                                                                 aTTTGAAAAGTGCTGCACACCACAG‑ACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑ATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGa                  <  4:456815/151‑1 (MQ=255)
                                                                                                                                    tGAAAAGTGCTGCAAAGCACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑ATTTGAAATTTCTATAAGTAACACACCCACCAAGCGGGAAt                >  8:1217384/1‑150 (MQ=17)
                                                                                                                                       aaaGTGCTGCAAAGCACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑ATTTGAAAGTACTGTAAGGACCAGAGCCCCCAAGCGGGTTTAGcc            >  1:536168/1‑150 (MQ=17)
                                                                                                                                       aaaGTGCTGCAAAGCACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑ATATTAAAGAGATATAATTAACAGACCCCCCAAGCgcgtaaagcg            >  2:1404185/1‑142 (MQ=14)
                                                                                                                                        aaGTCCTACAAATCACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑ATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTc           <  6:1591053/151‑1 (MQ=255)
                                                                                                                                          gTGCTGCAAAGCACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑ATATTAAATTACTAAAATCTACACACCACCCAAGCGGGataatcta           >  3:1434285/1‑142 (MQ=14)
                                                                                                                                                cAAAGCACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑ATTTTAAATAAATATAATGGACACAGCCACCAAGGGGGAATAGGGCAGTTgttt   >  3:896288/1‑148 (MQ=11)
                                                                                                                                                    |                                                                                                                                                     
CCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAG‑ACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAA‑ATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAG  >  NC_000913/4392309‑4392604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑