Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,187,667 T→A F773Y (TTC→TAC)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,187,6670TA99.1% 1715.2 / 0.8 447F773Y (TTC→TAC) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base T (2/2);  new base A (216/226);  total (219/228)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.38e-01

Only 100 of 448 total aligned reads displayed.

ATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCATGAT  >  NC_000913/4187519‑4187814
                                                                                                                                                    |                                                                                                                                                   
   atgGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACAtct                                                                                                                                                <  1:486292/151‑1 (MQ=255)
      gCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTcca                                                                                                                                             <  2:228754/151‑1 (MQ=255)
         gACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTccaccc                                                                                                                                          >  1:1558186/1‑151 (MQ=255)
         gACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTccaccc                                                                                                                                          >  2:84568/1‑151 (MQ=255)
         gACTCCGGTGCGCGTGGGTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTTGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTccaccc                                                                                                                                          <  1:942615/151‑1 (MQ=255)
             ccGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTccacccac                                                                                                                                        <  6:26893/149‑1 (MQ=255)
             ccGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACgg                                                                                                                                      <  4:1171301/151‑1 (MQ=255)
             ccGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACg                                                                                                                                       <  1:846190/150‑1 (MQ=255)
               ggTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGAACTACATCTCCACCCACGGTg                                                                                                                                    <  8:242886/151‑1 (MQ=255)
                  gcgcGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTc                                                                                                                                 >  5:1765343/1‑151 (MQ=255)
                    gcgTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGt                                                                                                                               >  7:942242/1‑151 (MQ=255)
                      gTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTa                                                                                                                              >  8:598470/1‑150 (MQ=255)
                        ggTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTaa                                                                                                                             >  6:1059234/1‑149 (MQ=255)
                         gTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAgg                                                                                                                          >  1:1714624/1‑151 (MQ=255)
                          ttCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGt                                                                                                                         >  4:770619/1‑151 (MQ=255)
                           tCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTc                                                                                                                        >  6:217874/1‑151 (MQ=255)
                              gCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTgg                                                                                                                     <  2:410471/151‑1 (MQ=255)
                              gCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTgg                                                                                                                     <  1:1855453/151‑1 (MQ=255)
                              gCGGCACAGATTCGTCAGCTTGCTGGTATGCGGGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTg                                                                                                                      <  3:773090/150‑1 (MQ=255)
                                   acaGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGAt                                                                                                                <  6:835340/151‑1 (MQ=255)
                                   acaGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGAt                                                                                                                >  4:1073573/1‑151 (MQ=255)
                                   acaGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGAt                                                                                                                >  3:44715/1‑151 (MQ=255)
                                    caGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGAt                                                                                                                >  8:516654/1‑150 (MQ=255)
                                          cGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCa                                                                                                          <  5:1640992/150‑1 (MQ=255)
                                          cGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCAc                                                                                                         >  3:1042411/1‑151 (MQ=255)
                                           gTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACt                                                                                                        >  5:735239/1‑151 (MQ=255)
                                            tCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTCAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTg                                                                                                       >  3:59035/1‑151 (MQ=255)
                                            gcAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTg                                                                                                       <  3:674375/150‑1 (MQ=255)
                                             cAGCTTGCTGGTATGCGTGTTCTGATGGCGAAGCGGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCAGTTAAGGTGTGAACGTACTCCAGTACTACATCTCCACGCACGGTGCTCGTAAATCTCTGGCGGATACGGCACTGa                                                                                                      <  5:1466221/151‑1 (MQ=255)
                                             cAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTg                                                                                                       >  8:1215348/1‑150 (MQ=255)
                                             cAGCTTGCTGGTATGCGTGGGCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGGCTGGCGGATACCGCACTGa                                                                                                      <  8:1638002/151‑1 (MQ=255)
                                              aGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGaa                                                                                                     <  4:1327923/151‑1 (MQ=255)
                                                cTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGaaa                                                                                                    >  1:1153123/1‑150 (MQ=255)
                                                 ttGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAAc                                                                                                  >  2:1807594/1‑151 (MQ=255)
                                                  tGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGaaaa                                                                                                   <  3:1684198/149‑1 (MQ=255)
                                                   gCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTg                                                                                                >  3:1123050/1‑151 (MQ=255)
                                                     tGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCg                                                                                              >  5:502938/1‑151 (MQ=255)
                                                       gTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGaa                                                                                            <  6:1396169/151‑1 (MQ=255)
                                                           gCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTcc                                                                                        <  7:1882218/151‑1 (MQ=255)
                                                            cGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCg                                                                                       >  4:542690/1‑151 (MQ=255)
                                                                   tGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCt                                                                                >  2:1118792/1‑151 (MQ=255)
                                                                    gATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTg                                                                               <  7:1470664/151‑1 (MQ=255)
                                                                       ggCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGAc                                                                             <  1:418269/150‑1 (MQ=255)
                                                                        gCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACtc                                                                           >  4:47466/1‑151 (MQ=255)
                                                                          gAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACtcgt                                                                         >  2:1736138/1‑151 (MQ=255)
                                                                          gAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACAGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACtcgt                                                                         >  7:1661966/1‑151 (MQ=255)
                                                                          gAAGCCGGATGGCTCCATCATCGAAACGCCAATAACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGCTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTAACATGACtcgt                                                                         >  2:638580/1‑151 (MQ=255)
                                                                           aaGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACtcgtc                                                                        >  3:902641/1‑151 (MQ=255)
                                                                            aGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACtcgtcg                                                                       >  3:1611315/1‑151 (MQ=255)
                                                                            aGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTCGCGGATACCGCACTGAAAACTGCGACCTCCGGTTACCTGACtcgtcg                                                                       <  8:1077456/151‑1 (MQ=255)
                                                                              ccGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCgtcgtc                                                                     >  3:1191473/1‑151 (MQ=255)
                                                                                     gCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTgc                                                              >  5:1186975/1‑150 (MQ=255)
                                                                                      cTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGAc                                                             >  1:182313/1‑151 (MQ=255)
                                                                                         catcatCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTg                                                          <  8:1427025/151‑1 (MQ=255)
                                                                                         catcatCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTg                                                          >  8:1829838/1‑151 (MQ=255)
                                                                                         catcatCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTg                                                          >  1:1440987/1‑151 (MQ=255)
                                                                                          atcatcGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTgg                                                         <  8:719403/151‑1 (MQ=255)
                                                                                          atcatcGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTgg                                                         >  4:1512675/1‑151 (MQ=255)
                                                                                          atcatcGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTgg                                                         >  3:1773917/1‑151 (MQ=255)
                                                                                            catcGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGgcg                                                       <  7:1308224/151‑1 (MQ=255)
                                                                                              tcGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGgcgc                                                      >  4:315388/1‑150 (MQ=255)
                                                                                               cGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAg                                                    <  2:1503996/151‑1 (MQ=255)
                                                                                                gAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAgg                                                   >  1:249807/1‑151 (MQ=255)
                                                                                                  aaCGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGAc                                                 >  6:1306321/1‑151 (MQ=255)
                                                                                                   aCGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGAcc                                                <  8:1768773/151‑1 (MQ=255)
                                                                                                   aCGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGAcc                                                <  3:1504777/151‑1 (MQ=255)
                                                                                                       cAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCtggt                                            <  3:1369588/151‑1 (MQ=255)
                                                                                                          tCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTggtggt                                         >  6:1638057/1‑151 (MQ=255)
                                                                                                           cACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGtt                                        <  8:308978/151‑1 (MQ=255)
                                                                                                               gcgAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCg                                    <  2:1057049/151‑1 (MQ=255)
                                                                                                               gcgAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCg                                    <  5:323000/151‑1 (MQ=255)
                                                                                                                 gAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGaa                                  <  6:372243/151‑1 (MQ=255)
                                                                                                                 gAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGa                                   >  1:656482/1‑150 (MQ=255)
                                                                                                                 gAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGa                                   >  5:1232768/1‑150 (MQ=255)
                                                                                                                  aaCTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAg                                 <  6:604782/151‑1 (MQ=255)
                                                                                                                   aCTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGa                                   >  4:1644167/1‑148 (MQ=255)
                                                                                                                    cTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGAc                               >  2:757638/1‑151 (MQ=255)
                                                                                                                      tCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGCCCTGGTGGGTACCGAAGACg                              >  1:340179/1‑150 (MQ=255)
                                                                                                                      tCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGAc                               <  4:1867808/149‑1 (MQ=255)
                                                                                                                          tGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATtgt                         >  5:1415791/1‑151 (MQ=255)
                                                                                                                           gAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATtgtg                        <  7:544144/151‑1 (MQ=255)
                                                                                                                             aGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGt                      <  8:1681466/151‑1 (MQ=255)
                                                                                                                                tCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTAcc                   >  7:34440/1‑151 (MQ=255)
                                                                                                                                  tGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGAAGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTAcca                  >  5:8674/1‑149 (MQ=255)
                                                                                                                                   gAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCa                 <  2:1186134/150‑1 (MQ=255)
                                                                                                                                    aaCGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATg               <  1:101330/151‑1 (MQ=255)
                                                                                                                                      cGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGaa             <  6:1545455/151‑1 (MQ=255)
                                                                                                                                      cGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGaa             <  4:642192/151‑1 (MQ=255)
                                                                                                                                      cGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGaa             <  3:1851129/151‑1 (MQ=255)
                                                                                                                                      cGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGa              >  7:1110940/1‑150 (MQ=255)
                                                                                                                                       gTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAg            <  3:292692/151‑1 (MQ=255)
                                                                                                                                        tACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAgg           >  4:944556/1‑151 (MQ=255)
                                                                                                                                         aCTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGt          <  8:640801/151‑1 (MQ=255)
                                                                                                                                             cAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCa      <  3:515844/151‑1 (MQ=255)
                                                                                                                                              aGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCat     <  7:748936/151‑1 (MQ=255)
                                                                                                                                              aGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCat     <  7:599479/151‑1 (MQ=255)
                                                                                                                                              aGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCat     >  7:1405959/1‑151 (MQ=255)
                                                                                                                                               gTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCatg    <  7:1854332/151‑1 (MQ=255)
                                                                                                                                                 aCTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCatgat  >  7:1602658/1‑151 (MQ=255)
                                                                                                                                                 aCTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCatga   <  6:1765789/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCATGAT  >  NC_000913/4187519‑4187814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: