Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,247,487 +A intergenic (+59/‑286) exuR → / → yqjA hexuronate regulon transcriptional repressor; autorepressor/general envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,247,4861.A97.1% 1720.3 / 31.7 454intergenic (+58/‑287)exuR/yqjAhexuronate regulon transcriptional repressor; autorepressor/general envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter
Reads supporting (aligned to +/- strand):  ref base . (6/7);  new base A (202/239);  total (208/246)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 482 total aligned reads displayed.

ACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTT  >  NC_000913/3247336‑3247631
                                                                                                                                                       |                                                                                                                                                 
aCCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGt                                                                                                                                                    <  1:154935/151‑1 (MQ=255)
   aGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGtaat                                                                                                                                                >  7:1505792/1‑149 (MQ=255)
   aGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGtaat                                                                                                                                                <  6:194996/151‑3 (MQ=255)
     cgacgaCTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGtaataa                                                                                                                                              <  5:1542533/151‑5 (MQ=255)
      gacgacTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGtaataa                                                                                                                                              >  3:522111/1‑146 (MQ=255)
      gacgacTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGtaataa                                                                                                                                              >  2:1705349/1‑146 (MQ=255)
      gacgacTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGtaataa                                                                                                                                              >  5:176504/1‑146 (MQ=255)
       acgacTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGtaataa                                                                                                                                              >  5:33307/1‑145 (MQ=255)
       acgacTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAg                                                                                                                                             <  8:17558/151‑1 (MQ=255)
        cgacTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAgt                                                                                                                                            >  4:462398/1‑151 (MQ=255)
         gacTTCGAGTTCAATGCCGACCGCTGTCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAgtg                                                                                                                                           >  5:1224357/1‑151 (MQ=255)
           cTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTc                                                                                                                                         >  2:1647287/1‑151 (MQ=255)
             tCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAATGTAATAAAGTGTc                                                                                                                                         >  3:704780/1‑149 (MQ=255)
             tCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCa                                                                                                                                        <  2:331583/150‑1 (MQ=255)
              cGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAg                                                                                                                                       <  8:1567768/150‑1 (MQ=255)
              cGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAg                                                                                                                                       <  6:1417352/150‑1 (MQ=255)
              cGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAg                                                                                                                                       <  7:194915/150‑1 (MQ=255)
              cGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGc                                                                                                                                      <  1:404130/151‑1 (MQ=255)
                  ttCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCtgt                                                                                                                                  >  5:935418/1‑151 (MQ=255)
                  ttCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCtgt                                                                                                                                  >  4:1576707/1‑151 (MQ=255)
                    cAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCtgtg                                                                                                                                 <  7:556958/150‑1 (MQ=255)
                    cAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCtgtg                                                                                                                                 <  8:1373270/150‑1 (MQ=255)
                         ccGACCGCTATCTATTCGCCGAAATCGCGGTGGTGCATCTCGATACAGCCACGATCGGCAGTAAATGAAATTCCTGTCAGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCTGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAAtg                                                                                                                           <  5:537903/151‑2 (MQ=255)
                         ccGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATc                                                                                                                           <  7:279407/151‑1 (MQ=255)
                         ccGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATc                                                                                                                           >  1:285923/1‑151 (MQ=255)
                           gACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCct                                                                                                                         >  1:952502/1‑151 (MQ=255)
                             ccGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCctct                                                                                                                       >  2:212598/1‑151 (MQ=255)
                              cGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCtctc                                                                                                                      <  2:129682/151‑1 (MQ=255)
                                  atctatTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGccc                                                                                                                  >  7:1585900/1‑150 (MQ=255)
                                  atctatTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGccc                                                                                                                  >  7:1225464/1‑150 (MQ=255)
                                    ctatTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCAcc                                                                                                                >  6:112947/1‑151 (MQ=255)
                                    ctatTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCAcc                                                                                                                >  2:1116658/1‑151 (MQ=255)
                                     tatTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCAccc                                                                                                               >  8:543040/1‑151 (MQ=255)
                                      atTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCAcc                                                                                                                <  4:940145/149‑1 (MQ=255)
                                      aatCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTGCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTATATCCTCTCGCCACCCt                                                                                                              <  7:68283/149‑1 (MQ=255)
                                        tCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGCCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTccc                                                                                                           >  6:379771/1‑151 (MQ=255)
                                           ccGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCt                                                                                                         <  2:1274610/151‑1 (MQ=255)
                                             gAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGc                                                                                                       >  6:1238838/1‑151 (MQ=255)
                                              aaaaCCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGGTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGc                                                                                                       >  4:1309857/1‑150 (MQ=255)
                                               aaaCCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCAt                                                                                                     >  3:647717/1‑151 (MQ=255)
                                                 aCCCGGTGGTGCATCTCGATACAGCCACCATCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTc                                                                                                   <  7:793747/151‑1 (MQ=255)
                                                 aCCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTc                                                                                                   <  1:492576/151‑1 (MQ=255)
                                                 aCCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTc                                                                                                   >  7:478452/1‑151 (MQ=255)
                                                 aCCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTc                                                                                                   >  4:337049/1‑151 (MQ=255)
                                                  cccGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGACAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCa                                                                                                  <  4:1126012/151‑1 (MQ=255)
                                                   ccGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAg                                                                                                 <  5:143781/151‑1 (MQ=255)
                                                     ggtggtGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGc                                                                                                >  6:735505/1‑150 (MQ=255)
                                                     ggtggtGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCa                                                                                               <  5:1641399/151‑1 (MQ=255)
                                                        ggtgCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCaaa                                                                                             <  8:256151/150‑1 (MQ=255)
                                                        ggtgCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCaaaa                                                                                            <  8:582260/150‑1 (MQ=255)
                                                            cATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCAGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATc                                                                                          >  3:1696005/1‑149 (MQ=255)
                                                                   aTACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCAc                                                                                 >  3:1176885/1‑151 (MQ=255)
                                                                   aTACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCAc                                                                                 >  1:739586/1‑151 (MQ=255)
                                                                    tACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACg                                                                                >  2:1447722/1‑151 (MQ=255)
                                                                       aGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCCTTCAGCAAAATCAGCCTCCACGGAc                                                                             >  6:1499612/1‑151 (MQ=255)
                                                                        gCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACGCTCCCCTGCATTCAGCAAAATAAGCCTCCACGGAc                                                                             >  3:1026158/1‑150 (MQ=255)
                                                                        gCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACa                                                                            <  4:639554/151‑1 (MQ=255)
                                                                         ccaccaGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACAt                                                                           <  3:1625318/151‑1 (MQ=255)
                                                                             caGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTa                                                                        >  3:1304074/1‑150 (MQ=255)
                                                                                 ggCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAAtttt                                                                   >  7:113190/1‑151 (MQ=255)
                                                                                  gCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTg                                                                  <  6:1050841/151‑1 (MQ=255)
                                                                                  gCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTg                                                                  >  1:1134505/1‑151 (MQ=255)
                                                                                  gCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTg                                                                  <  4:865200/151‑1 (MQ=255)
                                                                                    aGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGAt                                                                >  3:1458170/1‑151 (MQ=255)
                                                                                           gAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGaac                                                         <  8:1167384/151‑1 (MQ=255)
                                                                                           gAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGaac                                                         >  4:697188/1‑151 (MQ=255)
                                                                                            aaaTTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGaac                                                         >  3:937822/1‑150 (MQ=255)
                                                                                                tCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACg                                                     <  2:1032672/150‑1 (MQ=255)
                                                                                                 ccTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGt                                                    <  7:1408609/150‑1 (MQ=255)
                                                                                                 ccTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGt                                                    <  6:588890/150‑1 (MQ=255)
                                                                                                   tGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAGGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTg                                                  >  2:1312468/1‑150 (MQ=255)
                                                                                                    gTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTg                                                  <  5:1358051/149‑1 (MQ=255)
                                                                                                     tCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGAcc                                               >  5:627233/1‑151 (MQ=255)
                                                                                                       cGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCtt                                             >  8:695964/1‑151 (MQ=255)
                                                                                                        gACAGGCTCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGGGAAAAGTAATAAAGTGTCTGCGTGTGTAAATCCTCTCGCCTCCCTCCGGTCCATTCAGCAAAATCAGACTCCAGGGACATGTAATTTTGATAAGGAACAACGTTGACCttt                                            <  3:481980/151‑1 (MQ=255)
                                                                                                         aCAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCtttt                                           >  1:193094/1‑150 (MQ=255)
                                                                                                          cAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGt                                          >  2:482801/1‑151 (MQ=255)
                                                                                                            ggCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGCCTCCACGGACATGTAATTTTGATAACGAACAACGTTGACATTTGtt                                         >  2:701687/1‑150 (MQ=255)
                                                                                                               gcTGCAATGCGCCTGTTTGTCTCGTCGGGAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACaa                                     <  6:987445/151‑1 (MQ=255)
                                                                                                                 tGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCt                                              <  8:1030293/140‑1 (MQ=255)
                                                                                                                 tGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCt                                              >  1:1028951/1‑140 (MQ=255)
                                                                                                                 tGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAAtt                                   >  3:238837/1‑151 (MQ=255)
                                                                                                                  gCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTa                                  <  5:857546/151‑1 (MQ=255)
                                                                                                                   cAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAg                                 <  4:830174/151‑1 (MQ=255)
                                                                                                                    aaTGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGa                                <  5:1697870/151‑1 (MQ=255)
                                                                                                                     aTGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAAtt                                   >  4:746219/1‑147 (MQ=255)
                                                                                                                      tGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGAtt                              >  4:1419841/1‑151 (MQ=255)
                                                                                                                       gcgcCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGAtt                              <  5:716547/150‑1 (MQ=255)
                                                                                                                        ggcCTGTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCa                            <  5:461613/150‑1 (MQ=255)
                                                                                                                             gTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAAttt                        <  8:1209341/150‑1 (MQ=255)
                                                                                                                             gTTTGTCTCGTCGGTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTg                       <  8:1468145/151‑1 (MQ=255)
                                                                                                                                         ggTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAATTTATGtttt           >  3:404501/1‑151 (MQ=255)
                                                                                                                                          gTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAATTTATGttttt          >  8:912309/1‑151 (MQ=255)
                                                                                                                                          gTAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAATTTATGttttt          <  6:331715/151‑1 (MQ=255)
                                                                                                                                           tAAGCGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAATTTATGTTTTTg         >  2:90454/1‑151 (MQ=255)
                                                                                                                                               cGAAAAGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATg     >  8:442868/1‑151 (MQ=255)
                                                                                                                                                 aaaaGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGGATAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCt   <  6:889087/151‑1 (MQ=255)
                                                                                                                                                 aaaaGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCt   >  3:1719514/1‑151 (MQ=255)
                                                                                                                                                 aaaaGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCtt  >  4:1050005/1‑151 (MQ=255)
                                                                                                                                                  aaaGTAATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAATATATGTTTTTGAATGCtt  <  6:1613302/151‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                 
ACCAGCGACGACTTCGAGTTCAATGCCGACCGCTATCTATTCGCCGAAAACCCGGTGGTGCATCTCGATACAGCCACCAGCGGCAGTAAATGAAATTCCTGTCCGACAGGCGCTGCAATGCGCCTGTTTGTCTCGTCGGTAAGCGAAAAGTATAAAGTGTCAGCCTGTGTAAATCCTCTCGCCACCCTCCCCTGCATTCAGCAAAATCAGACTCCACGGACATGTAATTTTGATAACGAACAACGTTGACCTTTGTTACAATTAGATTCAATTTGAATTTATGTTTTTGAATGCTT  >  NC_000913/3247336‑3247631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: