Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913422,4290TG28.6% 657.3 / 87.4 378intergenic (+70/‑89)proY/malZproline‑specific permease/maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (62/205);  new base G (104/3);  total (169/209)
Fisher's exact test for biased strand distribution p-value = 5.32e-44
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 397 total aligned reads displayed.

GGTTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCT  >  NC_000913/422279‑422579
                                                                                                                                                      |                                                                                                                                                      
 ggttCGCGTGGATTGTTGTGCTGTTGATTGGCGGGATGTTTAACCGCCGCCACGAGCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtc                                                                                                                                                       <  6:1327154/149‑1 (MQ=255)
     cgcgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtcctc                                                                                                                                                    <  1:1030712/150‑1 (MQ=255)
         tGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCGCGTCCGCCTccc                                                                                                                                               >  2:815122/1‑151 (MQ=255)
               gttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGCCCCCaaa                                                                                                                                          >  7:1688014/1‑150 (MQ=255)
                 tgtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCACCCAAatat                                                                                                                                       >  6:1004489/1‑151 (MQ=255)
                    gCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATcc                                                                                                                                     <  5:1596585/150‑1 (MQ=255)
                         tGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCGGCCCCCAAATATCCTTGAgc                                                                                                                               >  4:248695/1‑150 (MQ=255)
                          gATTGGCGGCATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGCTCTCTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgat                                                                                                                              <  5:873152/151‑1 (MQ=255)
                                 tGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCGTCGGCCCCCAAAAAACCTTAACAAGAAGAGt                                                                                                                       >  1:1159939/1‑151 (MQ=255)
                                  ggATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGCGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCTTCAGATGATGAGTg                                                                                                                      >  8:1172207/1‑151 (MQ=255)
                                  ggATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGt                                                                                                                       <  8:1652550/150‑1 (MQ=255)
                                  ggATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGt                                                                                                                       <  4:118266/150‑1 (MQ=255)
                                   gATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGa                                                                                                                     <  8:908003/151‑1 (MQ=255)
                                    aTGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGAt                                                                                                                    <  3:977755/151‑1 (MQ=255)
                                    aTGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCGCCAAATATCCTTCAGATGAGGAGTgcg                                                                                                                    >  4:1051700/1‑149 (MQ=255)
                                      gTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGaggc                                                                                                                  >  4:975976/1‑148 (MQ=255)
                                        ttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCt                                                                                                                 <  4:1540856/150‑1 (MQ=255)
                                         tAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTg                                                                                                                <  8:1319908/150‑1 (MQ=255)
                                         tAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCACCCCCAAATATCCAACAGATGATGAGTGATccgcc                                                                                                               >  1:795802/1‑148 (MQ=255)
                                             cgccgcCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGTCGTCCTCCCCCAAATATCCTGCAGAGGATGTGTGATCCTgattt                                                                                                            >  3:843979/1‑146 (MQ=255)
                                                  ccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTa                                                                                                      <  4:760854/151‑1 (MQ=255)
                                                   cACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTGCGCCCCCAAATATCCTTCAGATGATGAGTGGTCGTGCATTAGGCTAt                                                                                                     >  6:1712840/1‑151 (MQ=255)
                                                   cACGATCGTCAGCTGGCTGAAAACCACTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGACTCCTGCCCCAAATATCCTTCAGATGATGAGGGATCCTCCATGAGCCTAt                                                                                                     >  4:466233/1‑151 (MQ=255)
                                                    aCGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGGCTCCTCCCCCCAATATCCTTCATATGATGAGTGATCCTGCATTAGGCTATg                                                                                                    >  4:557817/1‑151 (MQ=255)
                                                    aCGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCGGCCGCCCCCAGATATCCTTCAGATGATGAGTGAGGCTGCATTAGGCTATg                                                                                                    >  4:1695076/1‑151 (MQ=255)
                                                     cGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATgg                                                                                                   <  7:1309888/151‑1 (MQ=255)
                                                      gATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCGTCCCCCCAATATCCTTGAGATGATGTGTGCTCCTGCATTAGGGTATggg                                                                                                  >  2:3937/1‑150 (MQ=255)
                                                         cGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAAAAGCGTGCAGATGATGCGTGAGCCGGCATTGGGGTATGGCa                                                                                                 >  2:375606/1‑149 (MQ=255)
                                                            cAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCACAT‑TCCTGCGGATGATGTGTGTCCCTGCATTTGGCTTTGGGAACGTgt                                                                                           >  2:114984/1‑150 (MQ=255)
                                                              gctggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGAt                                                                                          <  2:743112/151‑1 (MQ=255)
                                                                tggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCGCCCCAATATCCGTCAGAGGATGAGTGGTCCTGGATTAGGGGTTGGGAGGGTGAtgc                                                                                        >  7:750301/1‑149 (MQ=255)
                                                                 ggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCa                                                                                        <  1:1222848/150‑1 (MQ=255)
                                                                     gAAATCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAttt                                                                                   <  6:1380024/151‑1 (MQ=255)
                                                                        aaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtca                                                                                <  7:689671/151‑1 (MQ=255)
                                                                          ccAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCCAATATCCGTAAGATGATGAGTGATCCTGCATTAGGGTATGGCAAGGTGATGAGATTTtaat                                                                               >  7:1070101/1‑150 (MQ=255)
                                                                             gTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATcaca                                                                           <  8:517962/151‑1 (MQ=255)
                                                                             gTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATcaca                                                                           <  7:1217962/151‑1 (MQ=255)
                                                                                aTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCTCCTCCCCCCAATATCCTTCAGCTGATGAGGGCTCCTGCATTAGGGGATGGGCAGGGGGTCAGCTTTTCATCCCAggg                                                                        >  4:723200/1‑151 (MQ=255)
                                                                                aTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAggg                                                                        <  8:252790/151‑1 (MQ=255)
                                                                                 accTTGCGGTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAATTTCATCACAgggg                                                                       <  8:372034/150‑1 (MQ=255)
                                                                                  ccTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgggg                                                                       <  3:403079/150‑1 (MQ=255)
                                                                                   cTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAAAAACATTAAGATGATGAGGGATCCTGCATTAGGCGATGGGAAGGTGATCAGATTTTAATCAAAGGGGa                                                                      >  1:1488369/1‑150 (MQ=255)
                                                                                    ttGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCAGCCCCCCGCCTCCAACCCCAAAAATCCTTCAGAAAAATAGTGATACTGGATTAACAAAACGCAAGGTGAACAGATTTTCAACAAAGGGGAAt                                                                    >  6:444640/1‑151 (MQ=255)
                                                                                     tccGTTCTGTAAGCCGGTGCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAAt                                                                    <  6:1192941/148‑1 (MQ=255)
                                                                                     tGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGaa                                                                     <  2:956883/149‑1 (MQ=255)
                                                                                     tGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAAt                                                                    <  8:1064858/150‑1 (MQ=255)
                                                                                     tGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCAGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCAAAGGGGAAtt                                                                   >  6:149001/1‑151 (MQ=255)
                                                                                       cGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTat                                                                 <  5:1157893/151‑1 (MQ=255)
                                                                                       cGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGGCGCCTCCCCCAACAATCCTTCAGATGATGAGTGGTGCTGCATTTGGGTATGGCAAGGTGATCAGATTTTACTCACAGGGGTAGTat                                                                 >  3:1317580/1‑151 (MQ=255)
                                                                                       cGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGAGGATGAGTGATGCTGCATTAGGGTATGGCTAGGTGCTCTGATTTTGATCGCAGGGGAAGTat                                                                 >  2:681570/1‑151 (MQ=255)
                                                                                        gTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGGCCCCAAATATCCTTCCGAGGGGGAGTGCTCCTGGCTTAGGCTGGGGCCCGGGGGTCAGCTTTTGCGGACAGGGGATTTatt                                                                >  4:660163/1‑150 (MQ=255)
                                                                                             gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGtt                                                           <  5:1463513/151‑1 (MQ=255)
                                                                                             gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGt                                                            <  8:60163/150‑1 (MQ=255)
                                                                                             gTAAGCCGGTTCAGGAGGAAATCCTGATACGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgatg                                                             <  6:153506/149‑1 (MQ=255)
                                                                                               aaGCCGGTTCAGGAGGAAATCCTGATACGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTa                                                          <  4:337944/150‑1 (MQ=255)
                                                                                                 gCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGGCCTCCGCCCCCCAATATCCTTAAGATGATGAGTGGTCCTGCATTAGGGTATGGCAAGGTGCTCAGATTTTCATCACAGGGGAATTatgagttttaat                                                       >  8:1019366/1‑143 (MQ=255)
                                                                                                       tCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATg                                                  <  2:1634548/150‑1 (MQ=255)
                                                                                                       tCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATg                                                  <  2:1468390/150‑1 (MQ=255)
                                                                                                       tCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCCAATATCCGTCAGATGATGAGTGAGGCGGCGTTAGGGGTTGGGGAGGGGATCAGCTTTTGATAACAGGGGAATTAGGATGTTATATGCAggg                                                 >  5:459161/1‑151 (MQ=255)
                                                                                                          ggaggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCAc                                              <  1:493358/151‑1 (MQ=255)
                                                                                                            aggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTGCTCCCCCAAAAATACATCAGATGGAGAGTGATCCTCCAGTAGGCGATGGCAACGTTATAAGATTTTGAAAACATCGGAATTAATATGGTAAAttactctcaccc                                            >  2:1332835/1‑140 (MQ=255)
                                                                                                             ggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCGCGTGCGCCTCCCCCCAACATACTACAGATGATGAGTGATCGTGCATTAGGCTGTGGGAAGGTGATGACATATTGATAACAGGCGAATTATGTTTTTAAATGCATGGCACCTg                                           >  2:234192/1‑151 (MQ=255)
                                                                                                             ggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTGCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATGAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCt                                            <  4:1114202/150‑1 (MQ=255)
                                                                                                              gaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGc                                          <  8:1475207/151‑1 (MQ=255)
                                                                                                               aaaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGcc                                         <  8:401791/151‑1 (MQ=255)
                                                                                                               aaaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGGGATCAGATTTTCATCACCGGGGAATTATGATGTTAAATTCCTGGCCCCTGcc                                         >  3:1461672/1‑151 (MQ=255)
                                                                                                                aaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCg                                        <  7:1579282/151‑1 (MQ=255)
                                                                                                                   ccTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGt                                      <  4:1370623/150‑1 (MQ=255)
                                                                                                                   ccTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGt                                      <  6:1663311/150‑1 (MQ=255)
                                                                                                                   ccTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTg                                     <  1:408993/151‑1 (MQ=255)
                                                                                                                   ccTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGCCCCCAAATATACTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTAATAACACGGGAAGTATGATGTTAAATTAATGGCAACAGCCCGTg                                     >  1:903729/1‑151 (MQ=255)
                                                                                                                   agtGATCCGGCTATTCCGAAAGTTATCGGCCCCCGTCCTCATCACCCAAATATGCTTCAGATGATGAGTGTTCCTGCATTAGGCTATGGCATGGTGATCAGTTTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCAGCTGCCGGt                                      <  1:1588396/148‑1 (MQ=255)
                                                                                                                     tGATCCGGCTATTCCGAAAGTTATCCGGCCCCGTCCTCCTCCCCCAAATATCCTTCAGAGGATAAGTCCTCCGGCATTAGGCTATGGCAAGGTGATCAGATTTTCATACCAGGGGAATTATGATGTTAAATGAATGGCACCAGGCGGTggc                                   >  8:743157/1‑149 (MQ=255)
                                                                                                                      gATCCGGCTATTCCGAAAGTTATCTGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGc                                    <  8:1098743/149‑1 (MQ=255)
                                                                                                                       aTCCGGCTATTCCGAAAGTTATCCGCCCCCGGCGGCCGCCCCCAAAAATAGTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGAGCAGATTTTCATCAAAGGGGAATTTTGATGTTAAATGCATGGCCCCTGCCGGGGcccc                                 >  5:756877/1‑151 (MQ=255)
                                                                                                                         ccGGCTATTCCGAAAGTTATCCGCCCCCCTCCTCCTCCCCCAAATATACTTCAGATGATGAGTGGTCCTGGATTAGGCTATGGCAAGGTGATCAGATTTTCATAACAGGGGAATTATGATGTTAAATGAATGGCCCCAGCCGGTGccccc                                >  4:159127/1‑150 (MQ=255)
                                                                                                                           ggCTATTCCGAAAGTTATCCGCGCCCGGCCGCCGCCCCCCAATATACTTCAGATGATGAGTGTTGCTGCATTAGGCTATGGCTAGGTGATCAGATTTTAATCACAGGGGAATTATGATGTTTAATGAATTGCACCTGCCGGTGCCCCCCtt                             >  3:1654412/1‑151 (MQ=255)
                                                                                                                            gCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCCAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTGATCACAGGGGAATTATTATGTTTAATTCATGGCCCCTGCCGGTGCCCCCAtct                            >  3:1160403/1‑149 (MQ=255)
                                                                                                                               aTTCCGAAAGTTATCCGCCCCCCTCCACCGCCCCCAAATATCCTTCAGATGATGAGTGTTCCTGCATTAGGCTATGGCAAGGTGATGACATATTAATCACAGGGGAATTATGATGTTAAATGCAAGGCCCCTGCCGCGGCCCCCAATTGtt                         >  1:329931/1‑151 (MQ=255)
                                                                                                                                 tCCGAAAGTTATCCGCCCCCGGCGGCCTCCCCCAAAAATCCTAAAGATGATGAGTGATAAAGAATTAAGCTAAGTCAAGGTGATCAGAATTTAATAAAAGAGCAATTATAATGTTAAATAAATTGCACCTGCAGGTGCCCCCAAATGttt                        >  6:859149/1‑149 (MQ=255)
                                                                                                                                  ccGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTaa                       <  6:1601557/150‑1 (MQ=255)
                                                                                                                                    gAAAGTTATCCGCCCCCGGCCGCGGCCCCCAAAGATCCTTCAGATGATGAGTGATGCTGCATTAGGCTATGGAAAGGTGATCAGATTTTGATCACAGGGGAATTATGATGTTTAATGCATGGCACCGGGCGGGGCCCCCCGTTGTTAAAc                     >  5:1304757/1‑150 (MQ=255)
                                                                                                                                       aGTTATCCGCCCCCGGCCGCCGCCCCCAAAAATCCGTAAGATGATGAGTGATCATGCATTAGGCTATGGGAAGGAGATCAGCTTTTAATCACAGGGGAATTATGATGTTAAATGAAAGGAACCGGCCGGTGCAGCCAGTTGTTAaaaaaac                 >  5:187126/1‑150 (MQ=255)
                                                                                                                                          tATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagca               <  1:1008572/150‑1 (MQ=255)
                                                                                                                                          tATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagca               <  8:669569/150‑1 (MQ=255)
                                                                                                                                           aTCCGCCCCCGGCCGCCGCCCCCAAAAATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTGATCACAGGGGAATTATGTTGTTAAATGCATGGCACCTGCCGGGGCCCCCATTTGTTAAAaaaagcaa              >  6:125187/1‑150 (MQ=255)
                                                                                                                                             ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGa           <  2:1086468/151‑1 (MQ=255)
                                                                                                                                             ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGa           <  7:626336/151‑1 (MQ=255)
                                                                                                                                              cGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCCGTGCCCCCATTTGTTAAAcaaagcaa              >  6:684764/1‑147 (MQ=255)
                                                                                                                                               gCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTGTGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaa              >  5:1689402/1‑146 (MQ=255)
                                                                                                                                               gCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATAACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAatat          >  4:607043/1‑150 (MQ=255)
                                                                                                                                               cccccccTCCTCCTCACCCAAATATCCCTCAGATGATGAGTGAGCCTGCATTCGGCGATCGCAAGGTGATCAGAGTTTCATCACAGGGGAATTATGATGTTAACTGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATc         <  5:926951/150‑1 (MQ=255)
                                                                                                                                                cccccGTCGTGGTCGCTCAAATATCGTTCAGTTGATGTGTGATCCTGGATTAGGCTATGGCAAGGTGATCAGATTTTCATCTCAGGGGATATATGATGTTTAATGCATGGGTCCTGCCGGTGCCCCCATTTGTTTAACAAAGCTAAGATCa        <  6:10810/151‑1 (MQ=255)
                                                                                                                                                 ccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCaa       <  6:730205/151‑1 (MQ=255)
                                                                                                                                                 ccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCa        >  7:1369117/1‑150 (MQ=255)
                                                                                                                                                  cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAAc      >  2:553545/1‑151 (MQ=255)
                                                                                                                                                  cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGGGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAAc      <  1:645730/151‑1 (MQ=255)
                                                                                                                                                   ccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt     <  3:255423/151‑1 (MQ=255)
                                                                                                                                                    cGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTg    <  6:57587/151‑1 (MQ=255)
                                                                                                                                                     gTCCTCCTCCCCCAAATATCCTTCAGACGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGc   >  7:408123/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
GGTTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCT  >  NC_000913/422279‑422579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑