Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,233,6960AC25.4% 664.6 / 55.4 293intergenic (+13/+32)fadH/higA2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked/antitoxinof the HigB‑HigA toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base A (164/53);  new base C (0/74);  total (164/129)
Fisher's exact test for biased strand distribution p-value = 8.73e-35
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 306 total aligned reads displayed.

GAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTT  >  NC_000913/3233546‑3233846
                                                                                                                                                       |                                                                                                                                                        
 agCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAg                                                                                                                                                         <  2:17893/151‑1 (MQ=255)
    cAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGCCGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAgcgc                                                                                                                                                      >  3:1746175/1‑151 (MQ=255)
    cAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAgcgc                                                                                                                                                      >  3:1231875/1‑151 (MQ=255)
     aaaCCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑ACCACGGCGTGCGCTGGAGATTTAAATCGTTATTTGAAGCGcc                                                                                                                                                     >  3:1314703/1‑151 (MQ=255)
      aaCCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCg                                                                                                                                                    >  8:642463/1‑151 (MQ=255)
        ccGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGa                                                                                                                                                  <  1:468768/151‑1 (MQ=255)
         ggcgcgcTGGCGCAAGCGCTGATTGATAGCGGGAAAACGGTGCATTTATTTGGCGGCTGCGATGTGGCTATGGAGCTGGAGGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGcggcggtt                                                                                                                                                 <  1:1144080/150‑3 (MQ=255)
         cgcgcgCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGAt                                                                                                                                                 <  4:852310/151‑1 (MQ=255)
         cgcgcgCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGAt                                                                                                                                                 >  8:1605360/1‑151 (MQ=255)
         cgcgcgCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGAt                                                                                                                                                 <  3:162795/151‑1 (MQ=255)
          gcgcgcTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATg                                                                                                                                                >  7:1293174/1‑151 (MQ=255)
            gcgcTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑a                                                                                                                                             <  8:399585/151‑1 (MQ=255)
             cgcTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑a                                                                                                                                             >  2:1264142/1‑150 (MQ=255)
                tGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGc                                                                                                                                         >  7:1011125/1‑151 (MQ=255)
                gggCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGc                                                                                                                                         <  7:302073/150‑1 (MQ=255)
                 gtcgcAAGCGGTGATTGATTGAGGGAAAACGTTGCATTTAATTGGCGGCTGCGATGTGGGTATGGAGCTGGACGCACGACGGGGAATTGCTCAGGG‑AACACGGCTGGCGGTGGAGATTTAAATCGTTTTTTGAAGCGCGGGATGC‑AACGc                                                                                                                                         <  1:1650424/148‑1 (MQ=255)
                 ggCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGc                                                                                                                                         >  2:135397/1‑150 (MQ=255)
                    gcAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATcc                                                                                                                                     >  4:1689733/1‑151 (MQ=255)
                     cAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCg                                                                                                                                    >  2:1115665/1‑151 (MQ=255)
                      aaCCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCgg                                                                                                                                   >  2:644428/1‑151 (MQ=255)
                      aaCCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCgg                                                                                                                                   >  6:1359320/1‑151 (MQ=255)
                         cGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCGGCAc                                                                                                                                >  4:453425/1‑151 (MQ=255)
                         cGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCGGCAc                                                                                                                                <  5:794137/151‑1 (MQ=255)
                          gCTGATTGATAGCGGGAAAACGGGGCATTTAATGGGCGGCGGCGATGGGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCGGCACg                                                                                                                               <  8:127458/151‑1 (MQ=255)
                           gtgattgaaAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGCACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCGGCACGt                                                                                                                              <  2:315904/150‑1 (MQ=255)
                             gattgatAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCGGCACGTcg                                                                                                                            >  8:17003/1‑151 (MQ=255)
                              attgatAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCGGCACGTcgc                                                                                                                           >  3:1700342/1‑151 (MQ=255)
                               ttgatAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATACGGCACGTagcg                                                                                                                          >  6:850891/1‑151 (MQ=255)
                                tgatAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCGGCACGTCGCGt                                                                                                                         >  7:447678/1‑151 (MQ=255)
                                 gatAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGCCGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCGGCACGTCGCGtt                                                                                                                        >  1:819701/1‑151 (MQ=255)
                                  atAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCGGCACGTCGCGtt                                                                                                                        >  8:297935/1‑150 (MQ=255)
                                  atAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCGGCACGTCGCGtt                                                                                                                        >  8:104948/1‑150 (MQ=255)
                                       gggAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCGGCACGTCGCGTTAATCa                                                                                                                   >  7:1657656/1‑150 (MQ=255)
                                          aaaaCGGTTCATTTATTTGGCGGCTGCTTTGTGGCTATGGTGCTGTACCCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCGGGATATTTAAATCTTTATTTTAAGCGCGGTATCA‑AACGCATCCGGCACGTCGCGTTAATCAATaa                                                                                                               <  7:1453776/151‑1 (MQ=255)
                                           aacgggttcttttAATTGGCGGGTGCGAGGGGGCTTGGGCGCTGCCCCCACGCCGGCAAATTCCCCTGGC‑AACCGGGGGGGCGTGGTTTATTTAAATGGTTTTTTCCCGCGCCGGATCC‑ACCCCAGCCGCCACGTCGCGTTAATCAATaaa                                                                                                              <  5:1182786/141‑1 (MQ=255)
                                               ggTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCGGCACGTCGCGTTAATCAATAAACa                                                                                                            >  2:141963/1‑149 (MQ=255)
                                                gTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCGGCACGTCGCGTTAATCAATAAACAAgg                                                                                                         >  5:1510083/1‑151 (MQ=255)
                                                  gCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGC‑g                                                                                                      >  4:962657/1‑151 (MQ=255)
                                                   cATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGCTGC‑AAAGCATCCCGCCCCTCGCGTTTATCAATAAACAAGGC‑gg                                                                                                     >  2:1551744/1‑151 (MQ=255)
                                                   cATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGC‑gg                                                                                                     >  2:449531/1‑151 (MQ=255)
                                                   cATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGC‑gg                                                                                                     >  4:51950/1‑151 (MQ=255)
                                                        aaTGGGGGGCGGGGAGGTGGCTCTGGCGGGGGCCGCACGCCGGGAAAGTGCCCCGGT‑AACCGGGCGGGCGCTGGTGATTTAAAGCGTTATTTGACGCGCGGGCTCC‑ACCGCAGCGGGCACGTCGCGTTAATCAATAAACAAGGC‑GGgaga                                                                                                 <  6:1608494/150‑1 (MQ=255)
                                                         aTTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGaa                                                                                                >  4:1528044/1‑150 (MQ=255)
                                                           tGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATg                                                                                             >  6:348996/1‑151 (MQ=255)
                                                             gcggcTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGcc                                                                                           >  4:321587/1‑151 (MQ=255)
                                                              cggcTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATTC‑AACGCATACGGCACGTCGCGTTAATCAATAAACAAAGC‑GGGAGAAATTccc                                                                                          >  6:1434404/1‑150 (MQ=255)
                                                                gcTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGCTGC‑AACGCATCCGGCCCGTCCCGGTAATCAATAAACAAGGC‑GGGAGCAATGCCGa                                                                                         >  3:937201/1‑150 (MQ=255)
                                                                 cTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAAt                                                                                       >  5:541083/1‑151 (MQ=255)
                                                                  ggCGTTGTGGCTATGGCGCTGCACCCACGAGGGCAAATTCCCCAGGT‑AACACGGCGGGCGGTGGATATTTAAATCTTTATTTGACGCGCCGGATGC‑AACCCATCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATc                                                                                      <  6:189518/150‑1 (MQ=255)
                                                                   gctaTGTGCCTTTGGAGCTGGACCCACGACGGGAAATTGCCCAGGC‑AACCCGGCTGGCGCTGGATATTTAAATCTTTTTTTCACGCGCCGTATGC‑AACCCACCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATcg                                                                                     <  2:989210/148‑1 (MQ=255)
                                                                    ggtgggggatatgtcgCTGGACGCACGCGGGGAAATGGCCCAGGC‑AACCCGGGGGGCGCTGGATATTTAAATCTTTTTTTGACGCGCGGGATGA‑ACCGCATCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATcgc                                                                                    <  8:1067384/136‑1 (MQ=255)
                                                                        gtgcctagtgtggtGGCCGAACGAGGGGATATTGCCCTGGT‑AACCCGGTTGCCGGTGTAAATTTAATTGTTTATTTTACGCGCC‑GTTGCAAACGCTCCCGCCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTg                                                                                <  5:908698/138‑1 (MQ=255)
                                                                        gtgGCTTTGGAGCGCGACGCACCAGGTGAAATTGCCCAGGC‑AACCCGGCTGGCGCTGGATATTTAAAGCGTTATTTCACCCGCCGGATCC‑AACGCAGCGGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGtt                                                                                 <  6:1053340/150‑1 (MQ=255)
                                                                         gggCTAGGGAGGGGGCCGCACGA‑GGGGAATTGCCCCGGGAAACCCGGCGGGGGCTGGATATTTAAATGTTTATTTGACGCGCGGGTTCC‑AACGCATCGGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGc                                                                               <  4:653021/150‑1 (MQ=255)
                                                                          ggcttggcaggtGGCCGCACGCGGGGCAATTCCCCTGGC‑ACCCGGGCTGGCGCTGTAGATTTAACTGTTTATTTGACGCGCCGGAGGC‑AACCAATCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGcc                                                                              <  3:276034/140‑1 (MQ=255)
                                                                            cTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTCTTTGAAGCGCCGGATGC‑AACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCa                                                                             >  4:1349275/1‑150 (MQ=255)
                                                                                 gAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCCGCACCTCGCCTTTATCAATAAACAAGGC‑GGGAGAAATGGAGAATCGCGTTGCCAAttttt                                                                       >  8:1432852/1‑151 (MQ=255)
                                                                                   gCTGCACCCACGACGGGAAATTGCCCAGGT‑AACCGGGCTGCGGCTTCATATTAAAATCGTTATTTCAAGCGGCGTATCA‑AAGCCATCGGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAAtttttt                                                                      <  6:623301/150‑1 (MQ=255)
                                                                                    cTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGACCCGGATGC‑AACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTac                                                                    >  5:1660408/1‑150 (MQ=255)
                                                                                     ggcccGCACGCCGGCCAATTGCCCAGGC‑AACCGGGCGGGCGCGGGAGATTAAAAGCTTTATTTCACCGGCGGGATGC‑ACCCCATCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGc                                                                   <  3:548633/147‑1 (MQ=255)
                                                                                      ggACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCGGCACCTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGGCGAATCGCGTTGGCAATTTTTTAGCg                                                                  >  3:780410/1‑151 (MQ=255)
                                                                                         cgaacgccgGGAATTTGCCCTGGG‑AACCGGGCGGGCGCTGTATTTTTAAATTTTTTTTTGACGCGGCGTTGGC‑AACGAAGCGGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCgtgt                                                               <  6:476254/148‑1 (MQ=255)
                                                                                         cGCACGCCGGGCAATGGCCCCGGC‑AACCCGGCGGGCGCGGGATATTTAAATCGTTATTTGACGCGCCGGATGC‑ACCGCATCGGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCgtgt                                                               <  8:889476/151‑1 (MQ=255)
                                                                                           cACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGTTGC‑AACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTc                                                             <  1:1326192/151‑1 (MQ=255)
                                                                                              gacgGGCAATGGCCCAGGG‑AACCCGGCGGGGGGTGTAGATTAAACTCTTTTTTTCACGCGCCGGACCC‑AACGCACCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAg                                                          <  8:1507143/151‑1 (MQ=255)
                                                                                              gacgGCAAATTGCCCAGGC‑AACCCGGCTGGCGCTGTATTTTTAAATCGTTATTTGACGCGCCGGATCA‑AACCCAGCGGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAg                                                          <  1:782796/151‑1 (MQ=255)
                                                                                                gggccAATTCCCCTGGC‑AACCCGGCGGGCGCTTTATATTTAAATCGTTATTTCACGCGCCGCATCC‑ACCCCACCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCg                                                        <  5:1363215/147‑1 (MQ=255)
                                                                                                    cAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTa                                                     >  3:210867/1‑150 (MQ=255)
                                                                                                     aaTGGCCCGGGG‑AACCCGGCTGGGCCTGGAGATTTAACTGGTTATTTCACGGGCCGGATCC‑AACCAATCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAAt                                                   <  2:1001230/151‑1 (MQ=255)
                                                                                                        tGCCGAGTG‑AACACGGCTGGCGCTGGAGTTTTTATGCTTTTTTTGACGCGCGGGATGA‑AAGGCTGGGGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAAttt                                                 <  4:1192464/150‑1 (MQ=255)
                                                                                                          cccTGGG‑AACCGGGGTGGCGTTGTACATTTAAATGTTTTTTTCAAGCGCGGGATCC‑AACGCAGCGGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCct                                              <  1:1466411/151‑1 (MQ=255)
                                                                                                           ccAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTAATTTGAAGCGCCCGATGC‑AACGCATACGGCACCTCGCGTTAATCAATAAACAAGGC‑GGGAGAAAAGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTGCAGCGTTATATTCctc                                             >  7:907069/1‑151 (MQ=255)
                                                                                                            cAGCG‑AACACGCCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGCATGC‑AACGCAACCGGCACGACGCGTTAATCAATAAACAAGGC‑CGCAGAAAAGCCGAATCGCATAGACAAATTTTTAGCGTCTTCCAGCGTTAATTTActa                                             >  1:566724/1‑149 (MQ=255)
                                                                                                                gcaaCCCGGCGGGCGCTGGAGATTAAAATCGTTATTTCACGCGCCGTATCC‑AACGCAGCCGCCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTcc                                         <  5:414896/149‑1 (MQ=255)
                                                                                                                 caaCCCGGGTGGCGGTGGAGATTAAAATCGTTATTTGAACCGCCGGAACC‑AACCCAGCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTccc                                        <  2:753717/150‑1 (MQ=255)
                                                                                                                  aaccgggctggctCTTTATATTTAATTCGTTATTTGACGCCCCGTATTA‑AACGATGCGGGCACGGCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCGAGCGTTAATTTCCTCTTCCCg                                       <  5:1050601/146‑1 (MQ=255)
                                                                                                                           ggCGCTGCATATTTAATTCGTTATTTCAAGCGCCGGATGC‑AACGAACCGGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAAcg                              <  6:1186139/151‑1 (MQ=255)
                                                                                                                            gcgcTGGCTATTTAAAGCGTTATTTCACGCGCGGGATGC‑ACCGCATCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAAcgc                             <  1:1020985/151‑1 (MQ=255)
                                                                                                                             cgcTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCACGCTCAAAAcgcg                            >  8:1263227/1‑151 (MQ=255)
                                                                                                                             cgcTGCACATTTAAATCGTTATTTGAAGCGCCCTAAGC‑AACGAATCCCGCACGTCCCGATAAACAATAAACAATCC‑CCGAGAAATTCCGAATCGCGATGCCAATTTATTAGACTCTACCCGCGTTAAATTACTATTCCACCTCAAAAcccg                            >  7:1067127/1‑151 (MQ=255)
                                                                                                                              gcTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGCATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGt                           >  3:1063195/1‑151 (MQ=255)
                                                                                                                               ggggAGATTTAAATCGTTATTTCAAGCGCGGGATGC‑AACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTg                          <  4:922817/149‑1 (MQ=255)
                                                                                                                               cTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTg                          >  1:309941/1‑151 (MQ=255)
                                                                                                                                tGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTg                          >  8:224551/1‑150 (MQ=255)
                                                                                                                                tGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCGGCACGTCGCGGTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTAGAGCGTTAATTTCCTCTTCCCGCTCAAACCGCGTgg                         >  1:1283741/1‑150 (MQ=255)
                                                                                                                                   agttttAATGCGTTATTTCAAGCGCCGGATGC‑AACGAAGCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGacac                      <  8:703145/148‑1 (MQ=255)
                                                                                                                                    gaTTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACAcc                     >  4:1091507/1‑151 (MQ=255)
                                                                                                                                     aTTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACAcc                     >  2:590184/1‑150 (MQ=255)
                                                                                                                                       ttaactgtttATTTGACGGGCCGGTGGC‑ACCGCACCGGCCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCAt                   <  4:1471212/142‑1 (MQ=255)
                                                                                                                                         aaaTCGTTATTTGAAGCGCCGGATGC‑AACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCAt                   >  5:260656/1‑148 (MQ=255)
                                                                                                                                             cGTTATTTGAAGCGCCGGATGC‑AACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGAttt             >  5:1632589/1‑150 (MQ=255)
                                                                                                                                             cGTTATTTCACCCCCCGGAGCC‑CACCCACCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGAttt             <  7:1371562/150‑1 (MQ=255)
                                                                                                                                              gTTATTTGAAGCGCCGGATGC‑AACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTAc           >  5:1266729/1‑151 (MQ=255)
                                                                                                                                              gTTATTTGAAGCGCCGGATGC‑AACGCAGCCCGCCCCGCGCGGTAATCAATAAACAAGGCGGGGGGAAATG‑CGAATCGCGGTGGCAATTTTTTAGCGTGTTGCCGCGGTTATTTAGTCTTCCCGCTGACAACGGGTGGCCCCAGAGATTTAc           >  7:772075/1‑151 (MQ=255)
                                                                                                                                              gTTATTTGAAGCCCCGGATTC‑AACGCATCCGGCACCTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTATCGTGTTCCAGCGTTAATTTCCTCTTCACGCTAAAAACGCGTGACAACATAGAATTa            >  1:159469/1‑150 (MQ=255)
                                                                                                                                                 aTTTGAAGCGCCGGATGC‑AACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACATCATAGATTTACTGc        >  7:1122392/1‑151 (MQ=255)
                                                                                                                                                    tGAAGCGCCCGATGC‑AACGCATACCGCAAGTCGCGTTAATAAATAAACAAGGC‑GGGAGAAATGCCGAATAGAGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATATCCTCTTCCCGATCAAAAACACTTACACCATAGAATTAcacaca      >  5:746378/1‑145 (MQ=255)
                                                                                                                                                      aaGCGCCGGATGC‑AACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAAtt   >  1:178965/1‑151 (MQ=255)
                                                                                                                                                      aaGCGCCGGATGC‑AACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAAtt   >  4:421184/1‑151 (MQ=255)
                                                                                                                                                       aGCCCCGGATGC‑AACGCATCCCGCACGTCCCGTTAATCAATAAACAAGGC‑GGGAGAAATGACGAATCGCGATGACAATTTTTTAGCGTGTTCCAGCGTAAAATTCCTCTTCCCGGTCATAACCCGTGACACCATACAATTACACCCAAtat  >  7:564394/1‑149 (MQ=255)
                                                                                                                                                       |                                                                                                                                                        
GAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGG‑AACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGC‑AACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGC‑GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTT  >  NC_000913/3233546‑3233846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑