Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,392,5690CA25.4% 367.0 / 13.7 214intergenic (+22/‑14)glyX/glyYtRNA‑Gly/tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base C (94/44);  new base A (43/4);  total (166/48)
Fisher's exact test for biased strand distribution p-value = 1.04e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 222 total aligned reads displayed.

CGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTG‑TAAGGCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTC  >  NC_000913/4392422‑4392719
                                                                                                                                                    |                                                                                                                                                       
cGTTTCCCGCTCAAAAATTTGAAAAGTGCTTCAAATCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTG‑TAAGGcaca                                                                                                                                                      <  7:542211/151‑1 (MQ=255)
 gTTTCCCGCTCCAAAATTTTAAAAGTGGTGAAAAGAACAGACAACCCAAGCGGGAATAGATAAGTTGGAAGAGAAAGACCTTGCCAAGGTCGGGGTCGGGAGATAGAGTCACGTTTCCCGCTCCAAATTGTGCACGGGCTG‑AAAGGgac                                                                                                                                                       >  1:963426/1‑149 (MQ=2)
 gTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCGAAAAGTTGAAAGTGCTG‑GAAGGccc                                                                                                                                                       >  2:509097/1‑147 (MQ=255)
    tCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGCCCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGCGTCTCGTTTCCCGCTCCAAAATGTGAACGTGGGG‑TCAGGccccgccc                                                                                                                                                  >  4:1322905/1‑144 (MQ=14)
        gCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTT‑TAATGGCCAGA‑cccccc                                                                                                                                             >  8:1143786/1‑151 (MQ=255)
         cTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGCTAGTGCTG‑TAAGGGCCAGa‑ccgccgc                                                                                                                                            >  2:165806/1‑149 (MQ=17)
          gccAAAATGTGAAAAGTGCTGCAAACCACAGACCACGCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTG‑TAAGGCACAGA‑CCACCCaa                                                                                                                                           <  8:1335429/150‑1 (MQ=255)
           ccAAAATTTGAAAAGTGCTTCAAATAACAGAAGACGCAAGCGGGATTAGCTCAGTTGGTATAGCACGACCTTGCCAAGGTCGGGTTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTG‑TAAGGCACAGA‑CCACCCa                                                                                                                                            <  7:165323/149‑1 (MQ=17)
           ccAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTG‑TAATGCACAGA‑CCCACCaac                                                                                                                                          >  3:974008/1‑150 (MQ=255)
             aaaaTTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATGTTCAAGGGCTA‑TAAGGGCCAGA‑cccccccaccg                                                                                                                                        >  2:944490/1‑146 (MQ=14)
              aaaTTTGAAAAGTCCTCCAAATAACAGACCACGCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTG‑TAAGGCACAGA‑CCACCCAAGCgg                                                                                                                                       <  1:1578161/151‑1 (MQ=255)
               aaTTTGAAAAGTTCTGCACACCACTGACGACCCACGCGGGAATAGCTCAGGTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGGCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTG‑TAAGGCACAGA‑CCACCCAAGCggg                                                                                                                                      <  7:1173086/151‑1 (MQ=17)
                 tttGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTTTGATCGGGATG‑TAATGGACAGA‑CCCCCCaccgggga                                                                                                                                     >  4:213233/1‑143 (MQ=14)
                  tggAAAAGTCCTCCACACCACATACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTG‑TAAGGCACAGA‑CCACCCAAGCGGGaa                                                                                                                                    <  8:348773/148‑1 (MQ=14)
                   tGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATATTAAAGAAATA‑AAAGACACAGA‑CCACCCAACCGGGaaa                                                                                                                                   >  7:240640/1‑149 (MQ=14)
                   tGAAAAGTCCTTCAAACCACAGACGACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCGCCAAAATTTGAAAGTGCTG‑TAAGGCACAGA‑CCACCCAAGCGGGAAt                                                                                                                                   <  5:1038967/150‑1 (MQ=255)
                     aaaaTTTCTCCAAATAACAGACGTCGCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCTAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTG‑TAAGGCACAGA‑CCACCCAAGCGGGAATAGc                                                                                                                                <  6:120519/151‑1 (MQ=14)
                     aaaaGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAACGTGCTA‑TAAGGCACAGAGCCACCC‑AGCGGGAATAgg                                                                                                                                >  5:1531654/1‑150 (MQ=17)
                       aaGTGCTCCAACGAAAATACCAACCAAGCGGCAAAAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTG‑TAAGGCACAGA‑CCACCCAAGCGGGAATAGCTc                                                                                                                              <  4:381426/151‑1 (MQ=14)
                        aGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTT‑TAATGAACAGA‑CCCCCCAAGAGGGAATAGCtgt                                                                                                                             >  3:1187523/1‑149 (MQ=17)
                          tgcttcAAATCACATAACACGCAAGTGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTG‑TAAGGCACAGA‑CCACCCAAGCGGGAATAGCTCAg                                                                                                                            <  2:1631483/150‑1 (MQ=14)
                          tgctgcAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGACCTA‑TAAGTAACACA‑CCACCCAACAGGGAAAAGCTAAGt                                                                                                                           >  1:1710703/1‑151 (MQ=17)
                           gctgcAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTTCTG‑TAAGCGACAGA‑CCGACCCAGGGGGCATAGcggcgtt                                                                                                                          >  8:214615/1‑145 (MQ=17)
                           gctgcAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTG‑TAAGGGACAGA‑CCCCCCAAGCGGGAATAGCTCAGtt                                                                                                                          >  3:1255336/1‑151 (MQ=255)
                           gctgcAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTG‑TAAGGCACAGA‑CCACCCAAGCGGGAATAGCTCAGt                                                                                                                           <  8:1435463/150‑1 (MQ=255)
                           gctgcAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCGCCAAAATTTGAAAGTGCTG‑TAAGGGCCAGA‑GCCCCCCAGCGGGGAAAGCGCAGtt                                                                                                                          >  3:1130193/1‑151 (MQ=17)
                             tgcAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTG‑TAAGGGACAGA‑CCCCCCACGCgggtttatggccgtttt                                                                                                                        >  8:553680/1‑137 (MQ=17)
                             tgcAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGGTTGCCCCTCCAAAATTTTAAAGTGCTG‑TAATGGACACC‑CCCCCCCACCGGGGATAggggaggttg                                                                                                                        >  3:1578229/1‑142 (MQ=14)
                               aaaataaaaGACGACCCACGCTGCAATACATAAGTTTTTCGTGCACGACATTGGCAAGGTCGTGTTCGAGATTTCGAGTCTCGTTTCCAGGTAGAAAATTTAAAAGTGCTG‑TAACCAACAGA‑CCACGCAAGCGGGAATAGCTCAGTTGGTa                                                                                                                      <  7:1529584/143‑1 (MQ=2)
                                aactctattaccgccCACGCGTGAAAAGATCATTTTTTCGCGCACGACATTGCCAAGGTCGGGTGCGCTACTTCGCGTCCCGTTACCC‑CGGACAAATTTGAAAATTCCGCTAACGAACATA‑CCAACCACGCGGGAATAGCTCAGTTGGTag                                                                                                                     <  2:562552/142‑1 (MQ=2)
                                aaaccaaataccaccCAAGCGGGAATAGCTCAGTTGGTTGAGCACGCCCTTGCCAAGGACGGGGTCGCGACTGCGCCTCTCGTTTCCAGCGCCAAAATTTCAAAGTGCTG‑TAAGCCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTag                                                                                                                     <  8:491280/142‑1 (MQ=14)
                                aaaGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATATGAAAGTACTA‑AAATGAACAGA‑CCAACCCAGAGCGAATAGCTCAGTTgttgt                                                                                                                     >  5:568470/1‑147 (MQ=14)
                                 aatcaaatagaactcAATCGGGAATATCTAATTTGTTAGAGCACTACATTGCCAAGGTCTGGTTCTCGATTTCGAGTCTCGTTTCCAGCTCGAAAATTTTAAAGTTCTG‑TAAGTCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTaga                                                                                                                    <  5:201104/137‑1 (MQ=9)
                                  aGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTTTGAAAGAGATC‑TAATGCACAGA‑CCCCCCCAGCGGGATTTGCGGAGTTTGTat                                                                                                                     >  7:863868/1‑148 (MQ=14)
                                     acaGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTTCAAGTGCTG‑TAATGAACAGA‑CCGCCCAAGGGCGATTAGGTCGGTTGGTAGAGccc                                                                                                                >  3:839220/1‑149 (MQ=14)
                                      caGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTTTGATAGGGCTG‑TAAGCGCCAGA‑CCCACCAAGCGGGAAAAGGTGAGTTTGTAGAGGACg                                                                                                               >  3:1026820/1‑151 (MQ=14)
                                      caGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGATATTAATA‑TAAGAAACACA‑CCGCCCAACAGCGAATATGAGAGTTGTTagagaaac                                                                                                               >  5:148922/1‑147 (MQ=11)
                                            aagtacgCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTG‑TAAGGCACAGA‑CCACCCAAGCGGGAAt                                                                                                                                   <  6:84777/119‑1 (MQ=21)
                                               cAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGAGCTA‑AAAGGCCCACA‑CCCCCCAACCGGGAATAGCGCAGTTGGTAGAGAaccaccgaacc                                                                                                       >  6:475219/1‑145 (MQ=11)
                                                 aGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGATC‑TAAGGCACACA‑CCACCCACGCGGGAATAGCGCAGTTGGTATAGCACGACCTTGCCa                                                                                                      >  4:221071/1‑149 (MQ=14)
                                                    gggAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTTTTAAAGTGCTG‑AAAGGCACAGA‑CCCCCCACGCGGGTATATCGCGGTTTGTTGTGGCCGAGCTTCCCAAGGtt                                                                                                 >  2:1030004/1‑150 (MQ=11)
                                                    gggAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATATGAAAGTGCTG‑TAAGGCACAGA‑CCACCCAAGAGGGAATAGCTCAGTTGGTAGAGCACGAGCGTGCCAAGGTc                                                                                                 >  6:60065/1‑151 (MQ=255)
                                                            cTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGATG‑TAATGAACAGA‑CCACCCAAGAGGGAATATCTCAGTTGTTAGAGAACGACCATGACAAGGTAAGGGTcac                                                                                         >  5:1385264/1‑149 (MQ=11)
                                                            cTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGAGCTC‑AAAGGAACAGA‑CCCCCCAACCGGGATTTGCTCCGTTGGTAGAGCACCACCATGACAAGTTccgcctcgg                                                                                         >  8:723175/1‑143 (MQ=11)
                                                             tCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGGACTG‑TAAGGGACAGA‑CCCCCCACCGGGGTATAGCTCAGTTGGTAGAGCACGAGAGTGCCAAGGGCCGCGTcgcg                                                                                        >  4:1532338/1‑151 (MQ=11)
                                                              cAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGATA‑TAATGCACAGA‑CCACCCAAGCGGGTATAGCGCAGTTGGTAGTGCAAGACCTTGACAAGGTCGGGGTcgcgt                                                                                       >  3:803672/1‑150 (MQ=11)
                                                              cAGTTGGTAGAGCACGACCTTACCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTTAAATTGATT‑AAATGAACAGA‑CCACCCAAACGGGAATATCAAAGTTTGTATAGACAGACCTAGCCAAGATctcggtag                                                                                          >  5:30941/1‑141 (MQ=2)
                                                               aGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGATG‑TAATTGACACA‑CCGCCCAAGGGGGTTTATGTGGGTTTGTAGAGCACCACAGTGCCAAGGGCAGGGTcgcttt                                                                                      >  4:271661/1‑148 (MQ=2)
                                                                 ttgttcgcgCAGGACCGTGCCAAGGTCGGGGGGGCTATTGCGAGTCCCGTTACCCGCCCCAAATTTTAAAAGTGCTC‑AACCCAACATA‑CCTCGCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGt                                                                                     <  8:844529/142‑1 (MQ=2)
                                                                 ttGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATATTAAATTTATA‑TAAGAAACACA‑CCACCCAAAAGCGAATATCGGAGTTTTTAGAGGAAGACCATGACAAgttaaaactcactaat                                                                                     >  6:186817/1‑135 (MQ=2)
                                                                    gTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTG‑TAAGGCACAGA‑CCCCCCCAGCGCGAATAGCGCCGTTGGTTGAGCACGACCGAGCCAAGGTGGGGGTCGCgtgtgccc                                                                                 >  2:1222694/1‑147 (MQ=11)
                                                                     tAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTG‑TAAGGCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCGTGCCAAGGTCGGGGTCGCGAGTTCg                                                                                  >  1:580510/1‑149 (MQ=38)
                                                                     tAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTG‑TAAGGCACAGA‑CCACCCAAGCGGGAATAGCTAAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCgcg                                                                                >  4:982/1‑149 (MQ=37)
                                                                      agagCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTG‑TAATGCACAGA‑CCCCCCACCCGGGAATAGGTCAGTTGGTAGAGGACGACCGTGCCAAGGTCGGGGTCGCGCGTTCGact                                                                               >  3:622321/1‑149 (MQ=11)
                                                                          cACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAATGGCTG‑TAAGGGCCAGA‑CCGCCCCAGCGGGTATATGGGCGTTGGTAGAGGACGACCGTGCCAAGGGCGGGGACCGGAGTTCgcggcggg                                                                           >  8:1023783/1‑144 (MQ=2)
                                                                             gACCTTGCGAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCAAAAATTTGAAAGTGCTG‑TAAGGCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGttt                                                                        <  3:156210/151‑1 (MQ=255)
                                                                              aCCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTTAAAGTGCTG‑TAAGGAACAGA‑CCACCCACGCGGGAATAGCGGGGTTGTTAGAGCACGACCATGCCAAGGTAGGGGTGGCGAGTTCGAGGCTCGTTTc                                                                       >  3:1443534/1‑151 (MQ=11)
                                                                                cTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTTAAAGTGCTG‑TAAGGGACAGA‑CCCCCCAAGCGGGAATAGCGGAGTTGGTAGAGCAAGACCGTGCCAAGGTGGGGGTCGCGAGTTCGCGTCGGGTTTcgg                                                                     >  8:1667001/1‑149 (MQ=11)
                                                                                  tgtctttgTCTGGTTCTCGAGTTCGAGTTGTTTTTCCGGTTCAAAAATTTTAAAGTGCTG‑TAAGGCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTc                                                                       <  7:1286421/140‑1 (MQ=11)
                                                                                   gCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTTAAAGTGCTG‑TAAGGGACACA‑CCCCCCCAGCGGGAATAGGGGAGTTTGTAGAGCACGACCTTGCCAAGGTGGGGGTCGCGTGTTCGAGTCTGGTTTCCGGCt                                                                  >  3:203279/1‑151 (MQ=11)
                                                                                      aaGGTCGGGGTCGCTAGTTCGAGTCTAGTTTCCCGCTCCAAATTTTGATAGTGCTG‑TATGTCACTGT‑CTACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCa                                                               <  1:536922/151‑1 (MQ=11)
                                                                                         gTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTTAAAGTGCTT‑TAAGGCACAGA‑CCACCCAACCGCGTATATCTCAGTTTGTATAGCACGACCGTTCCAATGTCGCGGTCGCTAGTTCGACTCTCGATTCACGATCCAAAt                                                            >  2:1106597/1‑151 (MQ=14)
                                                                                            ggggTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTTAAAGTTATG‑TAATGAACACA‑CCCCCCACGCGGGAATATGTGAGTTTTTGTAGCACCAGCGAGACAATTTCAGGGACGCGATTTCGCGTCTCGTTTCCCGATGCATAttat                                                         >  3:1709898/1‑149 (MQ=2)
                                                                                            ggcggcgcgAGCCCGCGGCTCGTTGCCCCCTCAAAATTTTTAAAGTGCTG‑TAATTCACATA‑CCACCCTAGCTGGAATAGCTCATTTTGTAGAGCACGACCTTGCCAAGTTCGGGGTGGGGATTTCTAGTCTCGTTTCCCGCTCCAAAttct                                                         <  6:1408947/146‑1 (MQ=2)
                                                                                                gcgcgACGTCGAGTCGGGTTGCCCGCTCAAAAATTTTAAAGTGCTG‑CAACGCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTtctc                                                     <  4:1435352/150‑1 (MQ=11)
                                                                                                 cgcgAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGATAGTGCTG‑TAAGGGCCAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTAGAGAACGACCGAGCCAAGGGCGTGGTCGCGAGTTCGCGGCGGGTGTCCGGCTCCATAATCTtctcg                                                    >  3:484340/1‑150 (MQ=14)
                                                                                                   cgAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTTAAAGTGATG‑TAATGGACAGA‑CCACCCAAGAGGGAATAGCTCAGTTTTTAGAGAACCACATTGACAAGGTCCGGGTGGCGAGTTCGACTCTGGTTTCGCGCTTCAAAttataatctc                                                   >  2:1464681/1‑142 (MQ=2)
                                                                                                    gAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTG‑TAAGGCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCTCGAGTTCGCCTCTCGTTTCCCGATGCAAATTCTTCTCTCa                                                  >  6:329381/1‑150 (MQ=14)
                                                                                                      gTTCGAGTCTCGTTTCCCGCTCCAAAATTTTAAAGTGATG‑TAAGGGCCAGA‑CCACCCAAGCGGGAATAGCGCAGTTTGTAGAGCAAGACCTAGCCAAGTTAGGGGTCGCTAGTTCGAAGCTAGTTTCGCTCTACaaaatattaaatcaat                                                >  5:364669/1‑137 (MQ=2)
                                                                                                      gTTCGAGTCTCGTTTCCCGCTCCAAAATTTTAAAGTGATG‑AAAGGAACACA‑CCACCCAACCGGGAATAGCGCAGTTGGTATAGAACCACCATGCCAATGTAGGGCACCCGAATACGAGGCAAGTGTCACGCTACAAAttataaaagaaat                                                >  6:1403466/1‑139 (MQ=2)
                                                                                                           aGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGATG‑TAAGTCACACA‑CCACCCAAGCGGGAATAGCTAAGTTTGTAGAGCACGACCTTGCCAAGTTCGGGGTCGCGAGTTCGAGTCTCGATTCCCGATCCAAATTCTTCTCTCAATAAAat                                           >  1:6429/1‑150 (MQ=17)
                                                                                                                cGTTTCCCGCTCCAAAATATGAAAGTGCTG‑AAAGCAACACA‑CCACCCAAGAGCGAATAGCTCAGTTGGTAGAGCACCACATTGCCAAGGTCCGCGTCGCGAGTTCGAATCTCGTTTCACGCTACAAAATCTTATATCAATAAAATAACca                                      >  5:1160954/1‑150 (MQ=14)
                                                                                                                 gTTTCCCGCTCCAAAATTTGAAAGTGCTG‑TAAGGCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCcac                                     >  1:22600/1‑150 (MQ=255)
                                                                                                                 gTTTCCCGCTCCAAAATTTGAAAGTGCTG‑TAAGGCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCcac                                     >  3:1314709/1‑150 (MQ=255)
                                                                                                                 gTTTCCCGCTCCAAAATTTGAAAGTGCTG‑TAAGGCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCcac                                     >  4:883257/1‑150 (MQ=255)
                                                                                                                 gTTTCCCGCTCCAAAATTTGAAAGTGCTG‑TAAGGCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCcac                                     >  4:1493037/1‑150 (MQ=255)
                                                                                                                  tttCCCGCTCCAAAATTTGAAAGTGCTG‑TAAGGCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCcac                                     >  8:939266/1‑149 (MQ=255)
                                                                                                                      ccccgcaaactattGACATTGCGG‑TCACGCAAAGA‑CCACCGTCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGAc                               <  3:353711/139‑1 (MQ=17)
                                                                                                                       cGCTCCAAAATTTGAAAGTGCTG‑TAAGGCACAGA‑GCACCCAAGAGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGAcc                              >  2:747375/1‑150 (MQ=255)
                                                                                                                        gCTCCAAAATTTGAAAGTGCTG‑TAAGGCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGAcgc                             >  1:1524212/1‑151 (MQ=255)
                                                                                                                         cTCCAAAATTTGAAAGTGCTG‑TAAGGCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGAcgcg                            >  4:1127988/1‑151 (MQ=255)
                                                                                                                         cTCCAAAATTTGAAAGTGCTG‑TAAGGCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGAcgcg                            >  6:1222774/1‑151 (MQ=255)
                                                                                                                          tCCAAAATTTGAAAGTGCTG‑TAAGGCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGAcgcg                            >  7:1432809/1‑150 (MQ=255)
                                                                                                                           ccAAATTTTTAAAGTGCTG‑TAACGCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGAt                          <  7:894809/151‑1 (MQ=255)
                                                                                                                           ccAAAATTTGAAAGTGCTG‑TAAGGCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTAGCGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGAt                          >  2:413367/1‑151 (MQ=255)
                                                                                                                             aaaaTTTGAAAGTGCTG‑TAAGGCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGc                        >  1:695274/1‑151 (MQ=255)
                                                                                                                              aaaTTTGAAAGTGCTG‑TAAGGCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCATCACTCAATAAAATATCCACATCGACGCGATGct                       >  6:753268/1‑150 (MQ=255)
                                                                                                                               aaTTTGAAAGTGCTG‑TAAGGCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTc                                                                       <  6:1007639/102‑1 (MQ=40)
                                                                                                                                    gAAAGTGCTG‑TAAGGCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTg                 >  8:260118/1‑151 (MQ=255)
                                                                                                                                       aGTGCTG‑TAAGGCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTg              >  7:778740/1‑151 (MQ=255)
                                                                                                                                         tGCTG‑TAAGGCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGt            >  2:244076/1‑151 (MQ=255)
                                                                                                                                          gCTG‑TAAGGCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATACTTCTCTCAATAAAAAATCCACAGCCACGCGAAGCGTTATTGcggctt           >  7:307384/1‑146 (MQ=18)
                                                                                                                                          gCTG‑TAACGCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGt            <  6:1440050/150‑1 (MQ=255)
                                                                                                                                             g‑TAAGGCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGCCGCGATGCGGTATTGGTGGttttt        >  1:304033/1‑151 (MQ=255)
                                                                                                                                             g‑TAAGGCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGttttt        >  2:880186/1‑151 (MQ=255)
                                                                                                                                               tAAGGCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTttg       >  6:1524625/1‑151 (MQ=255)
                                                                                                                                               tAAGGCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTttg       >  8:1404271/1‑151 (MQ=255)
                                                                                                                                               tAAGGCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTttg       >  2:1165663/1‑151 (MQ=255)
                                                                                                                                                aaGGCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTttgt      >  7:1563315/1‑151 (MQ=255)
                                                                                                                                                 aGGCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCTAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTttgtt     >  1:115743/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                       
CGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTG‑TAAGGCACAGA‑CCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTC  >  NC_000913/4392422‑4392719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑