Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,187,667 T→A F773Y (TTC→TAC)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,187,6670TA97.5% 1790.5 / 18.8 486F773Y (TTC→TAC) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base T (7/5);  new base A (249/225);  total (256/230)
Fisher's exact test for biased strand distribution p-value = 7.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01

Only 100 of 491 total aligned reads displayed.

ATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCATGATG  >  NC_000913/4187519‑4187815
                                                                                                                                                    |                                                                                                                                                    
  gatgGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGTACGTACTCCAGTACTACAtc                                                                                                                                                  >  1:1011173/1‑151 (MQ=255)
  gatgGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACAt                                                                                                                                                   <  6:742124/150‑1 (MQ=255)
   atgGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTCGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTATGTGAAGGTCTGAACGTACTCCAGTACTACAtct                                                                                                                                                 >  8:235604/1‑151 (MQ=255)
       ccGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTccac                                                                                                                                             <  6:1238840/151‑1 (MQ=255)
         gACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTccaccc                                                                                                                                           >  5:917850/1‑151 (MQ=255)
          aCTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACAGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTccaccc                                                                                                                                           >  6:375870/1‑150 (MQ=255)
             ccGGTGCGCGTGGTGCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTccacccac                                                                                                                                         <  5:398862/149‑1 (MQ=255)
               ggTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTg                                                                                                                                     <  3:1050394/151‑1 (MQ=255)
               ggTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTg                                                                                                                                     <  5:225336/151‑1 (MQ=255)
                   cgcgTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCg                                                                                                                                 <  6:1052907/151‑1 (MQ=255)
                     cgTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCAAGTACTACATCTCCACCCACGGTGCTCGt                                                                                                                                >  3:1617773/1‑150 (MQ=255)
                      gTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTa                                                                                                                               >  5:1469985/1‑150 (MQ=255)
                          ttCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGt                                                                                                                          >  3:942644/1‑151 (MQ=255)
                           tCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGt                                                                                                                          <  2:289970/150‑1 (MQ=255)
                             tGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTg                                                                                                                       >  6:202240/1‑151 (MQ=255)
                              gCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTc                                                                                                                         <  8:823109/148‑1 (MQ=255)
                               cGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCGAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGc                                                                                                                     >  7:1265625/1‑151 (MQ=255)
                               cGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGc                                                                                                                     <  3:293237/151‑1 (MQ=255)
                                ggCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCg                                                                                                                    >  6:525215/1‑151 (MQ=255)
                                ggCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCg                                                                                                                    >  8:795755/1‑151 (MQ=255)
                                  cacaGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCgg                                                                                                                   >  3:1286046/1‑150 (MQ=255)
                                     aGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATAc                                                                                                               >  7:1593546/1‑151 (MQ=255)
                                     aGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATAc                                                                                                               <  8:1005786/151‑1 (MQ=255)
                                      gATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATAcc                                                                                                              <  8:647481/151‑1 (MQ=255)
                                       aTTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCg                                                                                                             >  4:1676587/1‑151 (MQ=255)
                                           gTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACt                                                                                                         <  1:1622333/151‑1 (MQ=255)
                                             cAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTg                                                                                                        >  5:1358601/1‑150 (MQ=255)
                                               gCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGaaa                                                                                                     <  2:1618720/151‑1 (MQ=255)
                                                cTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGaaa                                                                                                     >  6:140965/1‑150 (MQ=255)
                                                    cTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGc                                                                                                >  7:112216/1‑151 (MQ=255)
                                                     tGGTATGCGTGGTCTGATGGCTAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCg                                                                                               >  1:1289463/1‑151 (MQ=255)
                                                      ggTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCg                                                                                               >  6:497681/1‑150 (MQ=255)
                                                       gTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGa                                                                                              >  5:825199/1‑150 (MQ=255)
                                                        tATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGaa                                                                                             >  5:285063/1‑150 (MQ=255)
                                                         aTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTCAAAACTGCGAACt                                                                                           >  6:1093730/1‑151 (MQ=255)
                                                           gCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTcc                                                                                         <  7:1479977/151‑1 (MQ=255)
                                                           gCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTc                                                                                          <  4:1604914/150‑1 (MQ=255)
                                                           gCGTGGGCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTcc                                                                                         <  6:1695611/151‑1 (MQ=255)
                                                            cGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCg                                                                                        <  2:103949/151‑1 (MQ=255)
                                                             gTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCgg                                                                                       >  6:556105/1‑151 (MQ=255)
                                                             gTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCgg                                                                                       <  4:900994/151‑1 (MQ=255)
                                                                gTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGtt                                                                                     >  1:339391/1‑150 (MQ=255)
                                                                 tCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTAc                                                                                   >  2:956853/1‑151 (MQ=255)
                                                                    gATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTg                                                                                >  5:1635996/1‑151 (MQ=255)
                                                                     aTGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCAGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTg                                                                                >  2:1120483/1‑150 (MQ=255)
                                                                      tGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGAc                                                                              >  2:1469051/1‑151 (MQ=255)
                                                                        gCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACtc                                                                            >  2:417989/1‑151 (MQ=255)
                                                                         cGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACtcg                                                                           <  7:351721/151‑1 (MQ=255)
                                                                         cGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACtcg                                                                           >  7:265769/1‑151 (MQ=255)
                                                                          gAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACtcgt                                                                          >  4:1105357/1‑151 (MQ=255)
                                                                          gAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACtcgt                                                                          >  4:1006705/1‑151 (MQ=255)
                                                                           aaGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACtcgtc                                                                         >  4:140145/1‑151 (MQ=255)
                                                                             gCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTtgtcgt                                                                       <  4:795178/151‑1 (MQ=255)
                                                                             gCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTcgtcgt                                                                       <  5:1156593/151‑1 (MQ=255)
                                                                              ccGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCgtcgtc                                                                      <  3:455487/151‑1 (MQ=255)
                                                                               gggATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCt                                                                     <  3:120201/150‑1 (MQ=255)
                                                                               cGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCt                                                                     >  1:1513710/1‑151 (MQ=255)
                                                                               cGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCt                                                                     >  2:183010/1‑151 (MQ=255)
                                                                               cGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGAGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCgtcgtc                                                                      >  6:1585375/1‑150 (MQ=255)
                                                                                ggATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTg                                                                    >  1:5125/1‑151 (MQ=255)
                                                                                ggATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTg                                                                    >  4:349084/1‑151 (MQ=255)
                                                                                ggATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTg                                                                    <  3:1268707/151‑1 (MQ=255)
                                                                                 gATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGCAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTgg                                                                   <  2:1198250/151‑1 (MQ=255)
                                                                                 gATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTgg                                                                   >  8:1592600/1‑151 (MQ=255)
                                                                                     gCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATCCCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGtggc                                                               >  5:708129/1‑148 (MQ=255)
                                                                                       tCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACg                                                             >  8:1366171/1‑151 (MQ=255)
                                                                                        ccATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGAc                                                              <  2:497718/149‑1 (MQ=255)
                                                                                        ccATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGt                                                            <  3:645600/151‑1 (MQ=255)
                                                                                            catcGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGgc                                                         <  2:1361331/150‑1 (MQ=255)
                                                                                            catcGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGgcg                                                        >  3:150445/1‑151 (MQ=255)
                                                                                             atcGAAACGCCAATCACCGCGAACTTCAGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGgcgc                                                       <  8:1207628/151‑1 (MQ=255)
                                                                                              tcGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGgcgc                                                       >  7:1684782/1‑150 (MQ=255)
                                                                                                gAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAgg                                                    >  8:543284/1‑151 (MQ=255)
                                                                                                    cGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGAACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCt                                                <  7:884962/151‑1 (MQ=255)
                                                                                                      ccAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCtgg                                              >  1:896505/1‑151 (MQ=255)
                                                                                                       cAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCtggt                                             >  7:138224/1‑151 (MQ=255)
                                                                                                               gcgAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCg                                     >  2:888577/1‑151 (MQ=255)
                                                                                                                cgAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGa                                    <  4:133969/151‑1 (MQ=255)
                                                                                                                  aaCTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGa                                    >  6:305990/1‑149 (MQ=255)
                                                                                                                  aaCTTCCGTGAAGGTCTGAACGTACTCCAGTACTAAATCTCCACCCACGGTGCTCGTAAAGGTCTGGAGGATACCGCACTGAAAACTGCTAACTCCGGTTACATGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGa                                    >  8:1442049/1‑149 (MQ=255)
                                                                                                                     ttCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACg                               >  3:190502/1‑151 (MQ=255)
                                                                                                                     ttCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACg                               >  8:1167070/1‑151 (MQ=255)
                                                                                                                         gTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATtg                           <  2:851040/151‑1 (MQ=255)
                                                                                                                           gAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATtgt                          <  3:1451392/150‑1 (MQ=255)
                                                                                                                             aGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGt                       <  7:523083/151‑1 (MQ=255)
                                                                                                                                tCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTAcc                    >  7:684756/1‑151 (MQ=255)
                                                                                                                                   gAACGTTCTCCAGTACTACATCTCCACCCACGGTGGTCGTAAAGGTCTGGCGGATACCTCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTGTGGTTGACGTGGCGGAGGACGTGGTGGTTACCGAAGACGATTGTGGTACGCAt                 <  4:60049/151‑1 (MQ=255)
                                                                                                                                   gAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCAt                 >  1:781157/1‑151 (MQ=255)
                                                                                                                                      cGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGa               >  4:1481623/1‑150 (MQ=255)
                                                                                                                                       gTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGa               >  2:629729/1‑149 (MQ=255)
                                                                                                                                       gTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAg             <  1:246578/151‑1 (MQ=255)
                                                                                                                                       gTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAg             <  6:674342/151‑1 (MQ=255)
                                                                                                                                        tACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAgg            >  6:989919/1‑151 (MQ=255)
                                                                                                                                           tCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTAt         >  4:986789/1‑151 (MQ=255)
                                                                                                                                            ccAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATc        <  5:491474/151‑1 (MQ=255)
                                                                                                                                            ccAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATc        >  1:1100329/1‑151 (MQ=255)
                                                                                                                                            ccAGTACTACATCTCCACCCACGGTGCTCGTAAAGGGCTGGCGGATACCGCACTGAAACCTGCGAACTCCGGGGACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATc        <  5:1730765/151‑1 (MQ=255)
                                                                                                                                                 aCTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCatgat   >  5:362572/1‑151 (MQ=255)
                                                                                                                                                 aCTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCatgat   >  3:79251/1‑151 (MQ=255)
                                                                                                                                                    tCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCatgatg  <  4:1234987/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCATGATG  >  NC_000913/4187519‑4187815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: