Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913422,4290TG30.3% 577.0 / 101.4 361intergenic (+70/‑89)proY/malZproline‑specific permease/maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (59/192);  new base G (108/1);  total (168/193)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 379 total aligned reads displayed.

GTTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCT  >  NC_000913/422280‑422579
                                                                                                                                                     |                                                                                                                                                      
 tttCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGgcc                                                                                                                                                      >  3:1102479/1‑151 (MQ=255)
     gcgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGgcctccg                                                                                                                                                  >  7:674085/1‑150 (MQ=255)
        tGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCGGGCCGCGTccc                                                                                                                                               >  3:269984/1‑151 (MQ=255)
          gATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCgccgcc                                                                                                                                             >  5:973708/1‑151 (MQ=255)
           aTTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGGCCCGTCCGCCTgcccc                                                                                                                                             >  2:679851/1‑150 (MQ=255)
             tgttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGTCGTCCGCCCCCac                                                                                                                                           >  5:549445/1‑149 (MQ=255)
              gttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAat                                                                                                                                         >  7:947401/1‑151 (MQ=255)
                 gtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATc                                                                                                                                      <  6:1265496/151‑1 (MQ=255)
                    cTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCCAATATccgt                                                                                                                                   >  5:78078/1‑149 (MQ=255)
                     tGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCTCCTCCCCCAAATATCCtt                                                                                                                                   >  4:1268588/1‑150 (MQ=255)
                      gTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCa                                                                                                                                 <  1:322346/151‑1 (MQ=255)
                      gTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGGTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCa                                                                                                                                 <  8:1365001/151‑1 (MQ=255)
                        tGATTGGCTGGATGTTTAAACACCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAg                                                                                                                                <  4:1016314/150‑1 (MQ=255)
                         gATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAga                                                                                                                               <  6:660517/150‑1 (MQ=255)
                         gATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAga                                                                                                                               <  4:848854/150‑1 (MQ=255)
                            tGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGCCCCCAAATATCCTGCAgatgat                                                                                                                           >  2:1066638/1‑151 (MQ=255)
                             ggCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgatgat                                                                                                                           <  8:800304/150‑1 (MQ=255)
                             ggCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGatgatg                                                                                                                          <  7:1000148/151‑1 (MQ=255)
                             ggCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGatgatg                                                                                                                          <  6:784264/151‑1 (MQ=255)
                              gCTGGATGTTTAACCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGGTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGAtgatga                                                                                                                         <  3:348312/151‑1 (MQ=255)
                              gCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCTCCTGCCCCCCAGATCCTTCTGctgatgg                                                                                                                         >  6:600383/1‑150 (MQ=255)
                              gCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGAtgatga                                                                                                                         <  1:638301/151‑1 (MQ=255)
                              gCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAAAATCAGTCAGatgatg                                                                                                                          >  2:1188533/1‑150 (MQ=255)
                               cTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCCAATAGCCTTCAGttgatg                                                                                                                          >  1:15247/1‑149 (MQ=255)
                                 ggATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTg                                                                                                                      <  3:1471980/151‑1 (MQ=255)
                                 ggATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTg                                                                                                                      <  5:288429/151‑1 (MQ=255)
                                     gTTTAAACGCCGCCACGATCGTCAGCTGGCTAAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATc                                                                                                                   <  5:1102752/150‑1 (MQ=255)
                                       ttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCACTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAAACCTGATCCGGCTATTCCGAAAGTTAACCCCCCGCGGCCGCGACCCCCCAAAATCCTTCAGATGAGAGGTGATccac                                                                                                                >  6:163882/1‑149 (MQ=255)
                                         aaaCGCCGCCAAGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGc                                                                                                               <  4:487300/150‑1 (MQ=255)
                                          aaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCGCCTCCCCCCAATATCCGTCAGCGGCTGCGTGATCCTGccg                                                                                                             >  7:455845/1‑149 (MQ=255)
                                          aaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGAGCGTGCAt                                                                                                             >  4:470053/1‑151 (MQ=255)
                                             gccgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCTCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCAttgg                                                                                                          >  4:931026/1‑149 (MQ=255)
                                              ccgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGGTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAgg                                                                                                         <  5:1201406/151‑1 (MQ=255)
                                               cgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATGCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGc                                                                                                        <  5:1495992/151‑1 (MQ=255)
                                               ccccACGCACGTCAGCTGGCTGAAACCCACTACTCCGTGCGGTCTGTAAGCCGGTTCAGGAGGAACACCTGATCCCGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGc                                                                                                        <  4:1551966/149‑1 (MQ=255)
                                                gccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCCGCCGCCGCCCCCCAATATCCTTCAGATGATGAGTGCTCCGGCATTAGGCt                                                                                                       >  5:1189628/1‑151 (MQ=255)
                                                   aCGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATg                                                                                                    <  8:1272386/151‑1 (MQ=255)
                                                    cGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATgg                                                                                                   <  6:1182275/151‑1 (MQ=255)
                                                       tCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCCAATATCCGTCAGATGATGAGTGATCCTGGATTAGGCTATGGCa                                                                                                 >  1:922006/1‑150 (MQ=255)
                                                        cGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCCTCCTGCCCCCAAAATCCATCAGATTATTAGTGATGCTGCTTGAGGGTAtctca                                                                                                 >  3:851587/1‑145 (MQ=255)
                                                         gTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCACAAATCCTTCAGATGATGAGTGAGCCTGCAGTCGGCTATGGCAAgg                                                                                              >  2:1131140/1‑151 (MQ=255)
                                                          tCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGGGCGGCCGCCTCCCCCAAAGATCCTTCAGATGATGAGTGCTCCTGCTGTAGGCTATGGCTAggg                                                                                             >  6:812419/1‑150 (MQ=255)
                                                           cAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGt                                                                                             <  5:36719/150‑1 (MQ=255)
                                                            aGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGa                                                                                           <  1:1010272/151‑1 (MQ=255)
                                                            aGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATACTTCAGATGATGAGTGATCCTGCATTATGCTATGGCAAgggg                                                                                            >  7:913035/1‑148 (MQ=255)
                                                              ctggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGGTAGGGCTAGGTggtg                                                                                         >  2:419202/1‑148 (MQ=255)
                                                               tggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAAAATCCATCAGAAGATGAGTTCTCCTGGATTAGGCTGTGTGAAGGTGATc                                                                                         >  3:234507/1‑150 (MQ=255)
                                                                ggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATc                                                                                         <  6:1059888/149‑1 (MQ=255)
                                                                ggctgAAAACCAGTAATCCTTGCGTGCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCTAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCa                                                                                        <  3:1420615/150‑1 (MQ=255)
                                                                  ctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGANATCCTGATCCGGCTATTCNNNANNNTNNNNGNNNCNNNNNTCCTCNNCCAAATATCCTACAGATGATGAGTGATCCGGCATTAGGCGATGGCAAGGTGNTc                                                                                         >  3:438347/1‑147 (MQ=255)
                                                                    gAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAtt                                                                                    <  5:1551893/150‑1 (MQ=255)
                                                                              aaTGCTTGAGTTCTGTAAGCCGGTTCAGGAGGAAATCCTTATCAGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATGCTGCATTAGGCTATGGCAAGGTGATCAGATTTTGATCACAgg                                                                         <  7:391756/151‑1 (MQ=255)
                                                                              aaTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCTGCGCCCCCCCATATCGGTCAGATGAGGAGTGAGCCTGGAGTGGGGTATGGCTAGGGGGTCAGATTTtgatctgagg                                                                         >  1:760335/1‑146 (MQ=255)
                                                                              aaTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgg                                                                         <  6:1514592/151‑1 (MQ=255)
                                                                              aaTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgg                                                                         <  6:304487/151‑1 (MQ=255)
                                                                              aaTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgg                                                                         <  1:402814/151‑1 (MQ=255)
                                                                              aaTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgg                                                                         <  7:590438/151‑1 (MQ=255)
                                                                                 ccTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgggg                                                                       <  2:250523/150‑1 (MQ=255)
                                                                                 ccTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGa                                                                      <  1:254317/151‑1 (MQ=255)
                                                                                 ccTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGa                                                                      <  5:756213/151‑1 (MQ=255)
                                                                                 ccTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGa                                                                      <  8:212857/151‑1 (MQ=255)
                                                                                 ccTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGGCGCCGCCCCCCAATAACCTTCAGATGCGGAGTGGTCCTGCATGAGGCTAGGTCAAGGTGATCAGCTTTTCGTCACAggggc                                                                      >  6:834371/1‑150 (MQ=255)
                                                                                  cTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGATGCTGCATTAGGGTATGGCAAGGTGATCAGATTTTGATCACAGGGGa                                                                      >  7:796375/1‑150 (MQ=255)
                                                                                    tGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCGCCGCCCCCCACTAACCTGCAGATGATGAGTGATCCGGGAGTAGGGTATGGGAAGGGGATCAGATTTTGATCACAGGGGACtt                                                                   >  4:56933/1‑151 (MQ=255)
                                                                                    tGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAAt                                                                    <  8:1104243/150‑1 (MQ=255)
                                                                                    tGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAATATCCTTCAGATGATGAGTGATCGTGCATTAGGGTATGGGAAGGTGATCAGATTTTGATGACAGGGGAGtt                                                                   >  4:654274/1‑151 (MQ=255)
                                                                                    tGCGTTCTGTAAGCCGGTTCACGAGGAAATCCTGATCCGGCTATTCCGAAAGTACTCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGaa                                                                     <  4:1578447/149‑1 (MQ=255)
                                                                                      cGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGGGGCGGGCTCCTCCCCCCAGTGTCGTTCAGATGATGAGTGAGGGTGGATTAGGCTTTGGGTAGGTGATCAGATTTTGATGACAGGGGAATTat                                                                 >  4:1135637/1‑151 (MQ=255)
                                                                                         tCTGTANGCCGGTTCAGNAGGANATCCTGATCCGGCTATTCCGAANGTTATCCGCCNCCGTCCTCCNCCCCCAAATATCCTNCANATGATGAGTGATCCTGCATNAGGCTATGNCAAGGTGATCAGATTTTCATCACAGGGGAATTatga                                                               <  7:1153105/150‑1 (MQ=255)
                                                                                             tAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGGTCCTGCAGTAGGCGGTGGCAAGGTGATCAGCTTTTGTTCACAGGGGAATTTTgttgttt                                                          >  5:86010/1‑150 (MQ=255)
                                                                                              aaGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCGTTCAGATGTTGGGTGATGCTGCATTAGGCTATGGGAAGGTGTTGAGTTTTTGATGACAGGGGAATTatgtttttt                                                          >  5:916149/1‑144 (MQ=255)
                                                                                               aGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTaaa                                                        <  8:528747/151‑1 (MQ=255)
                                                                                                  cGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCGATGGCAAGGTGATCAGATTTTCATAACAGGGGAATTATGATGTTTAATGc                                                     >  5:342319/1‑151 (MQ=255)
                                                                                                    gTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCGCCCCCCAATATCAGACAGAAGAACAGTGAAGGTGCATAAGGCTATGGCAAGGTGCGCAGATTTTCATCAGAGGGGAATATTGATGTTTAATCCa                                                    >  7:289160/1‑150 (MQ=255)
                                                                                                    gTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCGTTAAGCTGATGAGTGATCCTGCATTTGGGTATGGCAAGGGGCTCAGATTTTAATCACAGGGGAATTATGATGTTTAATGCAt                                                   >  5:421700/1‑151 (MQ=255)
                                                                                                      tCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGCCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATg                                                  <  1:1255989/150‑1 (MQ=255)
                                                                                                       cAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGc                                                <  4:1413101/151‑1 (MQ=255)
                                                                                                       cAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGc                                                <  7:1201944/151‑1 (MQ=255)
                                                                                                       cAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATACTTAAGAAGATGAGTGATGCTGCATTAGGCTATGGCAAGGTGATCAGATTTTAATCACAGGGGAATTATGATGTTAAATGAATGGc                                                >  3:1016987/1‑151 (MQ=255)
                                                                                                          gaggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCAcc                                             <  2:34788/151‑1 (MQ=255)
                                                                                                                    tGATCCGGCTATTCCGAAAGTTATCCGGGGCCGGCCGCCTCCCCCCAAAATCCATCAGCTGAACCGTGATGCTGCATAAGCCTAAGGCAACGGCATAAGATTTTCTACACAGCGCAATTTTCTAGTAAAATGAATGGAAcgacccgaggcc                                   >  7:1425649/1‑140 (MQ=255)
                                                                                                                     gATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCCCCCCCAAATAACCTTCAGATGATGAGTGATCCTGCATAAGGCTATGGCAAGGTGATCAGATTTTCATAACAGGGGTATTTTGATTTTAAATGAATATCACCTGCCCGTGccc                                  >  8:442950/1‑151 (MQ=255)
                                                                                                                      aTCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGcccc                                 <  2:52342/151‑1 (MQ=255)
                                                                                                                             tATTCCGAAAGTTATCCGCGCCCGGCCGCCTCCCCCAAATATGCTTGAGATGTTGAGTGATCCTGCATTAGGGTATGGGAAGGTGATCAGATTTTGATCACAGGGGAATTATGATGTTATATGAATGGCACCAGGCGGTGCCCCCATTTGt                          >  3:1265386/1‑151 (MQ=255)
                                                                                                                              aTTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTg                           <  2:1011513/149‑1 (MQ=255)
                                                                                                                              aTTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTg                           <  6:1006076/149‑1 (MQ=255)
                                                                                                                              aTTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTg                           <  4:331939/149‑1 (MQ=255)
                                                                                                                                 ccGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTaa                       <  2:1251023/150‑1 (MQ=255)
                                                                                                                                   gAAAGTTATCCG‑CCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGAATGGCACCTGCCGGTGCCCCCATTTGTTAAAca                    >  8:634246/1‑150 (MQ=255)
                                                                                                                                      aGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaag                 <  4:980367/151‑1 (MQ=255)
                                                                                                                                        ttATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTAATCACAGGGGAATTATTATTTTAAATGCATTGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagc                >  5:1365893/1‑150 (MQ=255)
                                                                                                                                             cGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGAt          <  7:171529/151‑1 (MQ=255)
                                                                                                                                             cGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGAt          <  6:1452432/151‑1 (MQ=255)
                                                                                                                                               cccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCa        <  4:181432/151‑1 (MQ=255)
                                                                                                                                               cccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGGGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATc         >  1:1359044/1‑150 (MQ=255)
                                                                                                                                               cccccGTCCTCCTCACCCAAATATCCTTCAGATGATTAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCa        <  1:684922/151‑1 (MQ=255)
                                                                                                                                                  ccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt     <  3:598413/151‑1 (MQ=255)
                                                                                                                                                   cGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTg    >  5:1264610/1‑151 (MQ=255)
                                                                                                                                                    gTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGc   >  1:474868/1‑151 (MQ=255)
                                                                                                                                                    gTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGc   <  2:1545962/151‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
GTTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCT  >  NC_000913/422280‑422579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑