Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,708,8050TA26.1% 85.7 / 29.7 119R629R (CGT→CGArsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base T (35/33);  new base A (24/0);  total (76/43)
Fisher's exact test for biased strand distribution p-value = 3.22e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

CCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAAC  >  NC_000913/1708656‑1708953
                                                                                                                                                      |                                                                                                                                                    
ccAGAACAACATGATGATCCACGCAAATCCGCCGTAGAAGCCGCTATCGCCCGGGACAAATCGCGGAATCTGGAACATCAACAGGCTAATGCGGAACCAGAACAACAGGCCGATCCGCGCAAACCCGCCGTCGAAGCCGCTATTGCCCGTg                                                                                                                                                     <  3:788255/151‑1 (MQ=11)
 cAGAACCACTTGTTGATCCTCGAAAATCTGCCGTCGAAGCAGCTATTTCCCGTGCCAAAGCGCGCAATCTGGAACATCAACAGGATAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGc                                                                                                                                                    <  2:1348636/151‑1 (MQ=11)
 cAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAGCAGCGCGTGGGTTATGCGGAGCGAGAAGGAGAGGTGGATCCTCGCAGAGCGGGCGTGGAAGCGGCTATTGCCCGTgg                                                                                                                                                    >  1:240698/1‑150 (MQ=11)
       cacaGGTAGATCCGCGAAAACCTGCCGTAGAAGCAGCTATTGCCCGGGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGc                                                                                                                                              <  5:120635/150‑1 (MQ=11)
         caggctgATCCGCGAAAACCAGCCGTAGAAGCCGCTATTGCCCGGGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGGCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCAc                                                                                                                                            <  8:1109970/145‑1 (MQ=11)
         caGGTCGATCCGCGCAAACCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCAc                                                                                                                                            <  7:879057/151‑1 (MQ=255)
           gctctaTCCCCGCAAACCAGCCTTTTAATCCGCTATTGCCCGTGCCAAAGCGAGCAAGCTTGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGCTCTATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGc                                                                                                                                          <  4:58430/146‑1 (MQ=11)
             gcGATCCGCGCAAAGCCGCCGGCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCGGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCaa                                                                                                                                        <  8:241253/150‑1 (MQ=255)
               gATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCAGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGc                                                                                                                                      >  7:621348/1‑151 (MQ=255)
                atagtcgaatacgagccgTAGCAGCAGCTATTTCCCGTGACAAACCTCGGAAGCTGGAACAGCAACAGGCTACTGCGGAACCAGAACAAGTGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCt                                                                                                                                     <  6:1465619/137‑1 (MQ=11)
                aTCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAAGGGCGGAACAGCACACCTGGTAGCTCCGCGCACAGCTGCCGTCGGCGCCGCTCGTGCGCGGGCCAAAGCACGAAAGcg                                                                                                                                     >  7:95544/1‑150 (MQ=2)
                aTCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGGGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCAGTGGCAGCGGCTATTGCCCGCGCAAAGGccagaaaccg                                                                                                                                     >  5:1441930/1‑142 (MQ=11)
                  cctcgcAAAGCTGCCGTCGAAGGCGCTATCGACCGGGCCCACGCGCGCAAGCTGGAACACCAACAGGCTAATCCGGAACCAGAACAACTGGGCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTgg                                                                                                                                   <  8:606668/148‑1 (MQ=11)
                  ccGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCCCGCAAACTCGAACAGCAACAGGCTAATGCCGAACCAGAAGAACAGGTAGATACGCGCAAAGCGGCAGTAGAAGCCGCTATTGCCCGCGCAAAAGCaaaaaaaaacg                                                                                                                                   >  2:516162/1‑141 (MQ=11)
                   cgcgCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTAGATCCGCGCAAAGCAGCAGTCGAAGCCGCTATTGCCCGAGCGAACGCCAGCaaacacg                                                                                                                                   >  4:888920/1‑145 (MQ=11)
                    ccgcAAATCCGCCGTCGAAGCCGCTATTGCCCGGGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGaa                                                                                                                                 <  5:1481166/150‑1 (MQ=255)
                     ggtataagtgccgTAGAAGCAGCTATTGCAGGTGCCTAATCTGGCAATCTGGAAGAGCATCAGGCTAATGCGGAACCAGAATAAGTGGTGGATCCCCGCAAATCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGaa                                                                                                                                 <  2:961556/141‑1 (MQ=2)
                      gcAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCAGTCGAAGCGGCTATTGCCCGAGCCAACGCCAGCAAGCGGGacc                                                                                                                                >  7:1148789/1‑148 (MQ=11)
                      gcAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTAGATCCGCGCAAAGCAGCTCTCAACACCGGAATTGACCGAAACAAAGAACGCaatagtgaac                                                                                                                                >  2:530447/1‑142 (MQ=11)
                        aaaccagccgTAGAAGCCGCAATAGCCCGGGAGTACGGGCTCCAGGTGCAACTGCACCTGTCCACTGCTGACCCAGAACAACAGGCTGATCCCCGCAAACCTGCCGTCGAAGCCGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAg                                                                                                                              <  8:25786/144‑1 (MQ=2)
                        aaaGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACCGGCTAATGCGGGAGCGGAAGAGCAGGACGCTCCGCTCCACGCAGCACTCGCCGCCGCTATTGCCCGAGGCACCGCGCGAAAGATGGAACCGc                                                                                                                             >  5:1535970/1‑151 (MQ=2)
                        aaaGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGCTCGGACCCCGAAACGACGGAGCGGCAGACGCTATTGCCCGTGGCCAATCCCGCAAGCTGGatc                                                                                                                                >  8:682246/1‑146 (MQ=14)
                          aGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTAGATCACCGAAGAGCCTCCGTATAAGCAACAATTGACCCAGAAAAATATCTCAAGCTGGAACACCa                                                                                                                            >  4:866041/1‑150 (MQ=2)
                          aGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGAGAAACCTAGAAAATCGTCAGTCGAAACAGCTATTGCAATAGCCAAAAAACGCAAGCTCGAACataa                                                                                                                            >  2:1516956/1‑147 (MQ=9)
                           gccgccGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTAGATCCGCGCAAAGCGGCCGTCGAAGCCGCTATTGCCCGCGCGAAAGCCAGCAACCGGGACCAGCacc                                                                                                                          >  7:545466/1‑149 (MQ=11)
                                   agctgCCGCTAGTGCCCTGGCAAACCCTCGCAACCTGGCAGATCTTCAGGCTAATCCCGAACCACCACCAGTGGCGGATCCCCGCAAATCCGCCGTCGATGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAAt                                                                                                                  <  1:1377817/147‑1 (MQ=2)
                                     aaGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCGCAGGCCCACAAGCGGGCCCAGCAACAGGCTATTGc                                                                                                                >  6:83603/1‑151 (MQ=14)
                                         cGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTAGATCCGCGCAAAGCGGCAGTAGAAGCCGCTATTGCAAGCGCAAAAGCAAAAAAACACGAACAGCAAAAGACAAATgaagac                                                                                                            >  1:296182/1‑146 (MQ=14)
                                          gCTATTGGCCGTGCTAAAGAGCTGAAGCTGGAACATCAACAGGATAATGCGGAACCAGAAGAACAGGTCGATCCTCGTAAAGCCGCCGTTGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGc                                                                                                           <  1:778872/151‑1 (MQ=14)
                                             aTTGCCCGTGCCAAAGCCCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTAGATCCGCGCAAAGCCGCAGTAAAATCCGATAATCCACGTGAAAAAGCACGCAATCTGGAAAAGAAAAAGGCTAAAGCCGAGcc                                                                                                          >  4:1160506/1‑149 (MQ=14)
                                               tccccGGGCCACCGCCCGCAAGCTGGAACACCAACAGGCTAATGCGGAACCAGCACCCCAGGGCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATGGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa                                                                                                       <  8:1530344/148‑1 (MQ=11)
                                                     ggaaaaaccccgcAAGCTGGAACAGCAACAGCATAATGAGGAACCAGAACAACAGGTCGATCCCCGCAAACCCGCCGTCGAAGCAGCTATTGCCCGGGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAg                                                                                                <  8:153663/141‑1 (MQ=11)
                                                       ccAAATGGCGGAATCTGGAAGACCTAGAGGATAATTCGGAACCAGAAGAAGAGGTCGATCCTCGCAAAGCTGCCGTTGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGt                                                                                              <  4:52206/151‑1 (MQ=14)
                                                        ataccgggggaaGCTGGAAGAGCATCAGCCTAATGAGGACGCAGCAAACGTGGGGGATCCTCGGAAACCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCTCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTc                                                                                             <  3:900130/141‑1 (MQ=2)
                                                        aaaaccccggaaGCTGGAACAGCAACAGCCTAATCCGGAACCAGAACAACATGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTc                                                                                             <  8:456241/141‑1 (MQ=11)
                                                        aaaaGCGCGCAAGCTGGAACATCATCAGGATAATGCGGAACCAGAACAACTTGTCGATCCTCGCAAAGCAGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTc                                                                                             <  4:1033886/150‑1 (MQ=11)
                                                           aacgcggAATCTGGAAGACCAACAGGATACTCCCGAACCAGCAAAACATGGCGATCCGCGCAAAGCCGCCGTCGAAGCAGCTATTGCCCGGGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGAt                                                                                          <  6:1166484/149‑1 (MQ=14)
                                                            gcgcgcAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTAGCAGCGGCTATTGCCCGAGCGCACGCAAGAAAACGCGAACAGCAGCAGGCTGAAGACGAGCACTaacaactattcaca                                                                                          >  3:1403951/1‑142 (MQ=2)
                                                            agtgggaatcTGGAAGATCTACAGGAAAATCCCGAAGCAGAACACGTGGGCGATCCCCGGAAACCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCTCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATc                                                                                         <  5:1574642/142‑1 (MQ=2)
                                                              gcgcAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTAGATCCGCGCAAAGCGGCAGTAGAAGCGGCTATTGCCCGAGCGCAAGCAAAAAAACACGAACAGCAACAGGCTATAGCGGAGGAAGAAAAACAAGTCGAtagc                                                                                       >  1:345924/1‑148 (MQ=2)
                                                              cggcAAGCTGGAACAACATCAGGAAAATTACGAAGCAGAATACGATGGAGATCCTCGCAAACCCGCCGTTGAAGCAGCTATTGCCCGTGCCAAAGCTCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcg                                                                                       <  1:164237/149‑1 (MQ=2)
                                                               cgcAAGCTGGAACAGCAACAGTCTAATGCGGAACCAGAAGAACATGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgc                                                                                      <  4:619332/151‑1 (MQ=255)
                                                                gcAAACTGNAACNTCAACAGGATAATTACGAACCAGAAACACTTGGCGATCCCCGTAAACCCGCCGTCGAAGCAGCTATTGCCCGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgcg                                                                                     <  6:1251392/151‑1 (MQ=14)
                                                                 cAAGCTGGAACACCAACAGGATAATAAGGACCCAGAACCAGAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCCCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCgcgc                                                                                    <  6:1362383/151‑1 (MQ=11)
                                                                   accTGGAACACCTACAGGAAACTAAGGAACCAGAAACAGTTGTAGATCCGCGTAAACCCGCCGTCGAAGCAGCTATCGCCCGTGCCAAAGCCCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCaa                                                                                  <  8:1342004/149‑1 (MQ=11)
                                                                    gCTGGAACAACATCAGGCTAATGCGGAACCAGAACACCTGGGCGATCCCCGCAAACCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCaaa                                                                                 <  7:271284/151‑1 (MQ=11)
                                                                    ccTGGAACACCTACAGGCAACTCCGGACCCAGCACACCAGGGCGATCCGCGCAAACCCGCCGTCGAAGCAGCTATTGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCaaa                                                                                 <  6:1418694/150‑1 (MQ=11)
                                                                       ggAACATCAACAGGATAATGAGGAACCAGAATAAGAGGTCGATCCTCGCAAAGCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCTCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCaa                                                                                  <  4:344668/147‑1 (MQ=11)
                                                                          aCAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTGGATCCGCGCAAAGCCGCCGTAGAAGCGGCTATTGCCCGAGCGCAAGCCAGCTAGCAGGAGCAGCCCGCGGCTATGGACGACCGAGAGCGACTGGTCGATCCGCGCAGAgcgggg                                                                           >  6:766346/1‑147 (MQ=2)
                                                                            aGCAACAGGCTAATGCGGAACCAGAAGAACAGGTGGATCCGCGCAAAGCGGCAGTAGCCGCGGCTATTGCCCGCGCGCAAGCCCGCAACCACGCCCAGCAACAGGCTATAGACGAGCCATAACTACACGTAGATCAGCGCACAGCCGCAGt                                                                         >  2:860688/1‑151 (MQ=2)
                                                                            aGCAACAGGCTAATGCGGAACCAGAAGAACAGGTAGATCCGCGCAAAGCCGCAGTCGCAGCGGCTATTGCCCGCGCACACGCAAGCAACCGCGCACAGCAGAAGGGTCATCGCTCGGCAGAGCAACAGGTTGATCCGCACaaaccaggcgg                                                                         >  5:284592/1‑143 (MQ=2)
                                                                               aaCAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCGGCCGTAGCCGCGGCTATTGCCCGCGCAAAAGCCCGCAAACACGCCCAGCAACATCCGAAGCCCGTCCCGGAAGAACAGATTGATCCGAGCAAagcagcactagc                                                                      >  3:1364653/1‑145 (MQ=2)
                                                                                  aGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCCGCGCCAACGCACGAAAGCAGGAACAGCACCAGGCTAATGCCGAGCCCTAACACCAGGTCCCACCGCGAACAGCCGCAGtggccgc                                                                   >  7:274974/1‑145 (MQ=14)
                                                                                     cTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCAAAAGCACGCAAGCACGAACAGCAACAGGATAATGCCGAGCCAGAACAACAAGTAGATCCGCCCAAAGCCCCAGTAGCAGCCGc                                                                >  1:765831/1‑151 (MQ=14)
                                                                                      tAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCGAACGCAAGCAAGCGGGACCAGCACCAGGGTAATGACGAGCACGAAGAACCGCTGGCGCCGCGCAACGCCGCCGTTGACGACGCt                                                               >  5:1170067/1‑151 (MQ=2)
                                                                                      tAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCAGCAGTAGAAGCGGCTATTGCCCGAGCAAAAGCAAGCAACCAGGAACAGCAACAGGCTAAAAACGAGCCAGAACAACAGGTAAAACAGCGCAAAGCCGCAGTAGaacagaa                                                                >  1:615206/1‑145 (MQ=255)
                                                                                        acttaggAAGCAGAAAAACAGGCAGATCCGCGCAAAACTGCCGTCGAAGCAGCTATTGCCCGGGCCAAAGCTCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTa                                                              <  6:165524/146‑1 (MQ=11)
                                                                                               aaCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCCCGCAACCAGGACCAGCAACAGGATAATGCGCGGCCAGAACCACAGCTGGATACGCGCAAAGACGCCGTAGCCGCCGCTAGTGCCCg                                                       >  5:489369/1‑151 (MQ=11)
                                                                                                   agaagaACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAACGCACGCAAGCGGGAACAGCCACAGGCTAATGCGGCTCCAGAACAACAGGTCGATCCGCGCAAAGCGGCCGTCGACGCCGCTATTCCCCgcgcg                                                   >  8:852604/1‑150 (MQ=11)
                                                                                                   agaagaACAGGTCGATCCGCGCAAAGCCGCAGTCGAAGCCGCTATTGCCCGGGCCAAAGCACGCAAGCTCGACCAGCATCAGGCTACTGCGGAGCCAGAACAACAGGTGGATCCGCGCAAAGCAGCAGTCGCCGCGGCTATTGCCCgcgcg                                                   >  5:1336919/1‑150 (MQ=11)
                                                                                                     aagaaCAGGTCGATCCGCGCAAAGCCGCCGTCGACGCCGCTATTGCCCGAGCCAACGCCCGCAAGCTGGAACAGCACCAGGCTAATGCCGAGCCAGAACAACAGGTCGCTCCGCGCACCGCCGCCGTCGACGCGGCTATTGCCCGCGCCac                                                 >  6:23256/1‑150 (MQ=14)
                                                                                                      agaaCAGGTCGATCCGCGCAAAGCGGCAGTAGAAGCCGCTATTGCCCGCGCGAAAGCACGAAAGCTGGCACAGCAACAGGGTAATGCCGAGCCAGAACAACAGGTAGCTCCGCGCAAAGCTGCCGTAGAAGCCGCTATTGCCCGCGCCaa                                                 >  6:629960/1‑150 (MQ=14)
                                                                                                       gaaCAGGTCGATCCGCGCAAAGCCGCCGTAGAAGCCGCTATTGCCCGAGCCAAAGCACGCAAGCTCGAACAGCACCAGGATAATGCCGAGACAGACGAACAGCTCGATACGCGCAAAGAGGCAGTAGACGCCGCTATTGCCCGCGCaaacg                                               >  7:701912/1‑149 (MQ=11)
                                                                                                       atagtctacGATCCCCGGAAATCCGCCGTGGTACGAGCTATCTCCCGTGCATAAGCTAGCTATTTGGAACATCTACAGGATACTTCCGACCCAGAACAACTGGGGGATCCGCGCAAACCCGCCGTCGAAGCCGCTATTGCCCGAGCCaaa                                                <  5:1035645/142‑1 (MQ=2)
                                                                                                            ggTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCGGCAGTTGACGCGGCTATTGCCCGCGCAAACgcc                                             >  6:829521/1‑147 (MQ=14)
                                                                                                               cGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTGGATCCGCGCAAAGCGGCCGTCGACGCCGCTATTGCCCGCGcccacgcccgaaac                                       >  5:864502/1‑139 (MQ=11)
                                                                                                                gATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTTGCTCCGCGCACAGCGGCCGTCGCCGCGGCGATTGCCcgcgcgcacgcgcgcaccg                                      >  5:591425/1‑148 (MQ=11)
                                                                                                                gATCCGCGCAAAGCCGCCGTAGAAGCCGCTATTGCCCGAGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTTGCCGCGGCTATTGCCCGCGCAAACgccaaaaagc                                      >  1:1445368/1‑143 (MQ=11)
                                                                                                                  tCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTGGATCCGCGCAAAGCGGCCGTGGCCGCGGCTATTGCCCGCGCGCACGCCCGCaaagcg                                    >  8:5181/1‑148 (MQ=14)
                                                                                                                    cgcgCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCGGCAGTAGCCGCGGCTATTGCCCGCGCAAACGCCAGAAAACgggc                                  >  2:259980/1‑150 (MQ=11)
                                                                                                                    cgcgCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTAGAAGCGGCTATTGCCCGAGCCAACGCCAGCAAACcggc                                  >  6:341977/1‑150 (MQ=14)
                                                                                                                     gcgcAAAGCCGCCGTCGAAGCCGCTATTGCCCGGGCCAAAGCACGCAAACTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTAGCCGCGGCTATTGCCCGAGCGAAGgccaaaaaacccgcc                                 >  2:704549/1‑138 (MQ=14)
                                                                                                                      cgcAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGAAACAGGCTAATGCCGAACCAGAACAACAGGTAGATCCGCGCAAAGCGGCAGTAGCAGCGGCTATTGCACGAGCACAAgccaaaaaacacgaac                                >  4:1438659/1‑137 (MQ=11)
                                                                                                                       gcAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGACCCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTCGCAGCCGCTATTGCCCGAGCAAAAgcccaaaaacccgaac                                >  2:184131/1‑136 (MQ=14)
                                                                                                                               gccgTAGAAGCAGCTATTTCCCGGGACAACTCACGGAAGCTGGAACACCAACAGGATACTGCCGACCCAGAACAACAGGGGGATCCGCGCAAACCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAAcc                         <  8:759024/149‑1 (MQ=14)
                                                                                                                                 cgTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGCGAACAGCAACCGGCt                     >  3:1053930/1‑151 (MQ=255)
                                                                                                                                 cgTCGAAGCCGCTATTGCCCGACCCAAAGCACGCAAGCACGAACACCAACAGGCTAATGCCGACCCAGAACAACACCTCGCTCCGCGCAAAGCCGCCCTAGAACCCCCTAATCCCCGACCCAACGCCAACAAACACGAACAGAAAcacgc                      >  3:532432/1‑146 (MQ=9)
                                                                                                                                  ggagaagaagCTATTCCCCGGGCAAAACCACGCAAGCTGGAACAGCAACAGGCTAATCCCGACCCAGAACAACAGGGCGATCCTCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTa                    <  7:1120592/142‑1 (MQ=14)
                                                                                                                                  gTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTAGAAGCGGCTATTGCCCGCGCGCAAGCCAACAAACCCGCACAGCagcaggat                     >  4:294488/1‑143 (MQ=11)
                                                                                                                                   tCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGACCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGCCGCGGCTATTGCCCGAGCGAACGCCCCCAAACGCGAACAGCACCAGGCtc                    >  8:1058012/1‑149 (MQ=14)
                                                                                                                                    cGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTGGATCCGCGCAAAGCCGCCGTGGAAGCGGCTATTGCCCGCGCGCACGCCCCCAAACCGGCACAGCACCAGGGTaa                   >  6:1171573/1‑150 (MQ=14)
                                                                                                                                     gAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCGGCCGTCGAAGCCGCTATTGCCCGCGCCCACGCGCGCAAAGCGGCCCAGCCAGCGGCtgagg                 >  5:742411/1‑147 (MQ=14)
                                                                                                                                      aaGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCCGTAGACGCCGCTATTGCCCGAGCCCACGCCCGCAAACCGGCCCAGCAGCAGGCtcaacc                >  5:395888/1‑146 (MQ=14)
                                                                                                                                       aGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCGGCAGTGGAAGCGGCTATTGCCCGCGCGAACGCGCGCAAACGCGAACAGCACCAGGCtctagcc               >  7:1369805/1‑145 (MQ=14)
                                                                                                                                       aGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCGGCAGTCGAAGCGGCTATTGCCCGCGCGCACGCGAGCAAAGCGGAACAGCagcagggtcttgcg               >  8:551152/1‑138 (MQ=14)
                                                                                                                                       aGCCGCTATTGCCCGAGCCAAAGCACGCAACCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTGGATCCGCGCAAAGCAGCCGTAGAAGCCGCTATTGCCCGCGCAAAAGCAAAAAAACACGAACAGCacaaggataaag                 >  2:88888/1‑138 (MQ=11)
                                                                                                                                         ccGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCGGCCGTCGCAGCGGCTATTGCCCGCGCCCAAGCGCGCAAACGGGAACAGCACCAGGCTAATGCgg              >  6:687855/1‑150 (MQ=14)
                                                                                                                                         ccGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCGGCAGTGGAAGCCGCTATTGCCCGAGCGAAAGCCCGCAAACGCGCCCAGCAAAAGGCTCAtcacg              >  5:1164143/1‑146 (MQ=14)
                                                                                                                                         ccGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTAGCAGCCGCTATTGCCCGAGCAAAAGCCAGAAAACGCGAACAGCAGCAGGCTAATGAgg              >  2:1376593/1‑150 (MQ=14)
                                                                                                                                             tATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAAAGATAGGAAGAGCTCGATCCTCGCCAAGCGGCCGTACACCCCCGGAGTGCACGCGCCAAAGCCCGCAAGCTCGAACAGCGCCCggggcaggcggggggg         >  6:759043/1‑137 (MQ=2)
                                                                                                                                             tATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTCGAAGCCGCTATTGCCCGCGCAAACGCCCTAAAACCCGAACAGCAACAGGCTAAAGCGGAGcc          >  3:718330/1‑150 (MQ=14)
                                                                                                                                             tATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTAGCAGCCGCTATTGCCCGCGCACAAGCGAGCAAACCCGCCCAGCAAAAGGCTCATACCGAGcc          >  2:769832/1‑150 (MQ=14)
                                                                                                                                              aTTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTAGAAGCGGCTATTGCCCGCGCCAACGCCCCCAAAGGCGACCAGCACAAGGCTTATGCGGAGGCat        >  8:1139821/1‑150 (MQ=14)
                                                                                                                                              aTTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCGAAAGCGCGCAAACGGGCACAGCACCCGGCTCATGCGGAGcc          >  7:371834/1‑149 (MQ=255)
                                                                                                                                                  cccGCGCCAAAGCTCGCAAGCTGGAACATCAACATGCTAATGCCGACCCAGAACAACAGTTCGATCCGCGCAAAGCCGCCGTAGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCagaaga    <  4:75930/151‑1 (MQ=255)
                                                                                                                                                   ccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTGGATCCGCGCAAAGCCGCCGTGGACGCGGCTATTGCCCGCGCCCACGCCAGAAAACGCGCCCAGCACCCGGCTGTAGCCGAGCAagaaga  >  5:1115856/1‑150 (MQ=9)
                                                                                                                                                    cGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTGGATCCGCGCAAAGCCGCAGTAGCAGCCGCTATTGCCCGAGCGAAAGCCAGCAAACCCGAACAGCACCAGGCTAATGACGAGCCCGAAAAAc  >  3:1100269/1‑151 (MQ=14)
                                                                                                                                                    cGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCGAACGCCCGCAAACGGGCCCAGCACCAGGCTAATGCGGAGCCagaagacc  >  5:762328/1‑149 (MQ=14)
                                                                                                                                                    cGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTCGAAGCCGCTATTGCCCGCGCCAACGCCCGAAAACCCGAACAGCACAAGGCTAATCCCGAGCCagaac     >  3:1290216/1‑147 (MQ=11)
                                                                                                                                                      tGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCAGCCGTCGAAGACAAAATTACACCCGCCAGATCGCGAAAACTGTAACAGAAACAGGATAATGCGGAGCGAGACGAAc  >  1:1223500/1‑149 (MQ=11)
                                                                                                                                                      |                                                                                                                                                    
CCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAAC  >  NC_000913/1708656‑1708953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: