Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,708,8830CA28.0% 192.0 / 24.9 167V655V (GTC→GTArsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base C (86/22);  new base A (39/3);  total (139/28)
Fisher's exact test for biased strand distribution p-value = 5.45e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 171 total aligned reads displayed.

CTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAAT  >  NC_000913/1708741‑1709033
                                                                                                                                              |                                                                                                                                                      
cTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCAAAAGCACGCAAGCACGAACAGCAACAGGATAATGCCGAGCCAGAACAACAAGTAGATCCGCCCAAAGCCCCAGTAGCAGCCGc                                                                                                                                                >  1:765831/1‑151 (MQ=14)
 tAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCAGCAGTAGAAGCGGCTATTGCCCGAGCAAAAGCAAGCAACCAGGAACAGCAACAGGCTAAAAACGAGCCAGAACAACAGGTAAAACAGCGCAAAGCCGCAGTAGaacagaa                                                                                                                                                >  1:615206/1‑145 (MQ=255)
         aaCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCCCGCAACCAGGACCAGCAACAGGATAATGCGCGGCCAGAACCACAGCTGGATACGCGCAAAGACGCCGTAGCCGCCGCTAGTGCCCg                                                                                                                                       >  5:489369/1‑151 (MQ=11)
          cccAGAAAACCATGCCGATCCCCGGAAAACGGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCTCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGGCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGa                                                                                                                                      <  5:1038215/150‑1 (MQ=11)
             agaagaACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAACGCACGCAAGCGGGAACAGCCACAGGCTAATGCGGCTCCAGAACAACAGGTCGATCCGCGCAAAGCGGCCGTCGACGCCGCTATTCCCCgcgcg                                                                                                                                   >  8:852604/1‑150 (MQ=11)
               aagaaCAGGTCGATCCGCGCAAAGCCGCCGTCGACGCCGCTATTGCCCGAGCCAACGCCCGCAAGCTGGAACAGCACCAGGCTAATGCCGAGCCAGAACAACAGGTCGCTCCGCGCACCGCCGCCGTCGACGCGGCTATTGCCCGCGCCac                                                                                                                                 >  6:23256/1‑150 (MQ=14)
                agaaCAGGTCGATCCGCGCAAAGCGGCAGTAGAAGCCGCTATTGCCCGCGCGAAAGCACGAAAGCTGGCACAGCAACAGGGTAATGCCGAGCCAGAACAACAGGTAGCTCCGCGCAAAGCTGCCGTAGAAGCCGCTATTGCCCGCGCCaa                                                                                                                                 >  6:629960/1‑150 (MQ=14)
                 gaaCAGGTCGATCCGCGCAAAGCCGCCGTAGAAGCCGCTATTGCCCGAGCCAAAGCACGCAAGCTCGAACAGCACCAGGATAATGCCGAGACAGACGAACAGCTCGATACGCGCAAAGAGGCAGTAGACGCCGCTATTGCCCGCGCaaacg                                                                                                                               >  7:701912/1‑149 (MQ=11)
                      ggTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCGGCAGTTGACGCGGCTATTGCCCGCGCAAACgcc                                                                                                                             >  6:829521/1‑147 (MQ=14)
                         cGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTGGATCCGCGCAAAGCGGCCGTCGACGCCGCTATTGCCCGCGcccacgcccgaaac                                                                                                                       >  5:864502/1‑139 (MQ=11)
                          gATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTTGCTCCGCGCACAGCGGCCGTCGCCGCGGCGATTGCCcgcgcgcacgcgcgcaccg                                                                                                                      >  5:591425/1‑148 (MQ=11)
                          gATCCGCGCAAAGCCGCCGTAGAAGCCGCTATTGCCCGAGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTTGCCGCGGCTATTGCCCGCGCAAACgccaaaaagc                                                                                                                      >  1:1445368/1‑143 (MQ=11)
                            tCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTGGATCCGCGCAAAGCGGCCGTGGCCGCGGCTATTGCCCGCGCGCACGCCCGCaaagcg                                                                                                                    >  8:5181/1‑148 (MQ=14)
                              cgcgCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCGGCAGTAGCCGCGGCTATTGCCCGCGCAAACGCCAGAAAACgggc                                                                                                                  >  2:259980/1‑150 (MQ=11)
                                cgcAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGAAACAGGCTAATGCCGAACCAGAACAACAGGTAGATCCGCGCAAAGCGGCAGTAGCAGCGGCTATTGCACGAGCACAAgccaaaaaacacgaac                                                                                                                >  4:1438659/1‑137 (MQ=11)
                                 gcAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGACCCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTCGCAGCCGCTATTGCCCGAGCAAAAgcccaaaaacccgaac                                                                                                                >  2:184131/1‑136 (MQ=14)
                                         gccgTAGAAGCAGCTATTTCCCGGGACAACTCACGGAAGCTGGAACACCAACAGGATACTGCCGACCCAGAACAACAGGGGGATCCGCGCAAACCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAAcc                                                                                                         <  8:759024/149‑1 (MQ=14)
                                           cgTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGCGAACAGCAACCGGCt                                                                                                     >  3:1053930/1‑151 (MQ=255)
                                            gTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTAGAAGCGGCTATTGCCCGCGCGCAAGCCAACAAACCCGCACAGCagcaggat                                                                                                     >  4:294488/1‑143 (MQ=11)
                                             tCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGACCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGCCGCGGCTATTGCCCGAGCGAACGCCCCCAAACGCGAACAGCACCAGGCtc                                                                                                    >  8:1058012/1‑149 (MQ=14)
                                              cGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTGGATCCGCGCAAAGCCGCCGTGGAAGCGGCTATTGCCCGCGCGCACGCCCCCAAACCGGCACAGCACCAGGGTaa                                                                                                   >  6:1171573/1‑150 (MQ=14)
                                                aaGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCCGTAGACGCCGCTATTGCCCGAGCCCACGCCCGCAAACCGGCCCAGCAGCAGGCtcaacc                                                                                                >  5:395888/1‑146 (MQ=14)
                                                 aGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCGGCAGTGGAAGCGGCTATTGCCCGCGCGAACGCGCGCAAACGCGAACAGCACCAGGCtctagcc                                                                                               >  7:1369805/1‑145 (MQ=14)
                                                 aGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCGGCAGTCGAAGCGGCTATTGCCCGCGCGCACGCGAGCAAAGCGGAACAGCagcagggtcttgcg                                                                                               >  8:551152/1‑138 (MQ=14)
                                                 aGCCGCTATTGCCCGAGCCAAAGCACGCAACCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTGGATCCGCGCAAAGCAGCCGTAGAAGCCGCTATTGCCCGCGCAAAAGCAAAAAAACACGAACAGCacaaggataaag                                                                                                 >  2:88888/1‑138 (MQ=11)
                                                   ccGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCGGCCGTCGCAGCGGCTATTGCCCGCGCCCAAGCGCGCAAACGGGAACAGCACCAGGCTAATGCgg                                                                                              >  6:687855/1‑150 (MQ=14)
                                                   ccGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCGGCAGTGGAAGCCGCTATTGCCCGAGCGAAAGCCCGCAAACGCGCCCAGCAAAAGGCTCAtcacg                                                                                              >  5:1164143/1‑146 (MQ=14)
                                                   ccGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTAGCAGCCGCTATTGCCCGAGCAAAAGCCAGAAAACGCGAACAGCAGCAGGCTAATGAgg                                                                                              >  2:1376593/1‑150 (MQ=14)
                                                       tATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTCGAAGCCGCTATTGCCCGCGCAAACGCCCTAAAACCCGAACAGCAACAGGCTAAAGCGGAGcc                                                                                          >  3:718330/1‑150 (MQ=14)
                                                       tATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTAGCAGCCGCTATTGCCCGCGCACAAGCGAGCAAACCCGCCCAGCAAAAGGCTCATACCGAGcc                                                                                          >  2:769832/1‑150 (MQ=14)
                                                        aTTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTAGAAGCGGCTATTGCCCGCGCCAACGCCCCCAAAGGCGACCAGCACAAGGCTTATGCGGAGGCat                                                                                        >  8:1139821/1‑150 (MQ=14)
                                                        aTTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCGAAAGCGCGCAAACGGGCACAGCACCCGGCTCATGCGGAGcc                                                                                          >  7:371834/1‑149 (MQ=255)
                                                            cccGCGCCAAAGCTCGCAAGCTGGAACATCAACATGCTAATGCCGACCCAGAACAACAGTTCGATCCGCGCAAAGCCGCCGTAGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCagaaga                                                                                    <  4:75930/151‑1 (MQ=255)
                                                              cGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTGGATCCGCGCAAAGCCGCAGTAGCAGCCGCTATTGCCCGAGCGAAAGCCAGCAAACCCGAACAGCACCAGGCTAATGACGAGCCCGAAAAAc                                                                                  >  3:1100269/1‑151 (MQ=14)
                                                              cGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCGAACGCCCGCAAACGGGCCCAGCACCAGGCTAATGCGGAGCCagaagacc                                                                                  >  5:762328/1‑149 (MQ=14)
                                                              cGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTCGAAGCCGCTATTGCCCGCGCCAACGCCCGAAAACCCGAACAGCACAAGGCTAATCCCGAGCCagaac                                                                                     >  3:1290216/1‑147 (MQ=11)
                                                               gTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTGCCAGAAGAGCACGTAGCTCCGCGCCACGCAGCGGGCGACGCGGCTCGTGCGCGCGGCACAGCGCGCAAGCGGGAACAGAAACAGGCTCCTGCgggggcggccgcgcg                                                                                 >  6:1351188/1‑138 (MQ=2)
                                                                tGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCAGCCGTCGAAGACAAAATTACACCCGCCAGATCGCGAAAACTGTAACAGAAACAGGATAATGCGGAGCGAGACGAAc                                                                                  >  1:1223500/1‑149 (MQ=11)
                                                                  ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCGGCCGTAGAAGCCGCTATTGCCCGCGCCAAAGCGCCCAAAGGCGCACAGCACCCGCATATAGCGGAGCACgaagaagaagt                                                                              >  8:277473/1‑146 (MQ=14)
                                                                  ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGACCCAGAACAACAGGTAGATCCGCGCAAAGCGGCAGTAGACGCCGCTATTGCCCGCGCAAAAGCCAACAAACCCGACCAGCAACAGGATAAAAACGAGCAAGAAAAACAgtg                                                                              >  1:100356/1‑149 (MQ=2)
                                                                   cAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCGGCCGTAGAAGCCGCTATTGCCCGAGCCAACGCGCGCAAACCGGAACAGCAACAGGCTAATCCCGAGCAAGAACAACACGtt                                                                             >  6:896526/1‑151 (MQ=14)
                                                                     aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGACCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTAGAAGCCGCTATTGCCCGAGCCAAAGCGCAAAAACGCGACCAGCAACAGGCTAAACCGGAGCCAGAAAAACAgat                                                                              >  4:1135050/1‑146 (MQ=14)
                                                                          cGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCACAAAACAACACCGTCGAAGACGCTAATCCACTAGCCAAAGCTCTAAAACTCGAACAGCAACCGGATAATTAAAAAAAAGAAGAAGAGGGTGATCcgg                                                                      >  1:161527/1‑150 (MQ=9)
                                                                           gCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTGGATCCGCGCAAAGCGGCCGTGGAAGCCGCTATTGCCCGCGCTAAAGCCAGAAAACCGGACCAGCAGAAGGCTGATGCGGAGGCCGAAGAACACGtgcctaaccg                                                                     >  5:1480670/1‑142 (MQ=2)
                                                                               gCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAACGCGCGCAAACGGGAACAGCACCCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCaac                                                                 >  8:1290227/1‑150 (MQ=255)
                                                                               gCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTAGAAGCGGCTATTGCCCGCGCGAAAGCCAGCAAACGCGAACAGCAGAAGGATGATGACGAGCCATAAGACCAAGtaaatcatccaaac                                                                 >  3:551288/1‑138 (MQ=2)
                                                                                cTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCAGTAGACGCGGCTATTGCCCGAGCAAAAGCCAGCAAACCGGAACAGCAACAGGCTAAAGACGAGCAAGAAGATCAGGttcgtcggcgtag                                                                  >  2:954771/1‑138 (MQ=14)
                                                                                   gAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTAGAAGCCGCTATTGCCCGAGCAAAAGCCCGCAAACGCGAACAGCACCAGGATAATGCGGAGCCAGAAGAACACGATCAGCCGCGCAAAGCTg                                                             >  1:220581/1‑151 (MQ=14)
                                                                                    aaCAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTAGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCAACAGGCTAATCCGGAGCCAGAATAAGAGGTTGATACGCGCAAACCTGc                                                            >  5:295982/1‑151 (MQ=14)
                                                                                       aGCAACAGGCTAATGCGGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAg                                                                                                              <  5:1398781/98‑1 (MQ=255)
                                                                                        gCAACACGCTAATGCCGATTAAGAACTACAGGTTTATCCTCGAATAGGCTCTTTCGATGTAGCTATTGCCCGAGCCAAAGCGCGCAATCGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTc                                                        <  4:73420/151‑1 (MQ=18)
                                                                                         cAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTAGAAGCGGCTATTCCCCGCGCCACAGCGCGCAACCGGGGACAGCAAGCGGCTAATGCGGTGCCAGAAGAACAGGTTGATACGCGCAAAGCTGCCGtgg                                                       >  6:1486868/1‑149 (MQ=14)
                                                                                          aaCAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCAGTCGAAGCCGCTATTGCCCGCGCAAAAGCGAGAAAACGCGAACAGCAACAGGCTAAAGCGGAACCAGAACAACAGGTTGATCCGCGCAAACCTgaagtagc                                                      >  3:1549820/1‑144 (MQ=11)
                                                                                          aaCAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCAGTAGAAGCCGCTATTGCCCGAGCCAAAGCGCGAAAACCGCCACAGCAACCAGCTAATGCGTAGCCAGAAGAACAGGGTGCTCCGCGCAAAGCTGCAGTCg                                                       >  8:1290442/1‑150 (MQ=17)
                                                                                           aCAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGACCAGGTTGATCCGCGCAAAGCTGCAGTCGac                                                     >  4:412844/1‑150 (MQ=255)
                                                                                             aggctacttaggAGTCAGAACACCTTGTGGATCCTCGTAAAGCTGCCGTAGAAGCAGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACAGGATAATGCGGATCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGc                                                   <  4:1215960/140‑1 (MQ=17)
                                                                                             aGGTTAATAACGAGGGAGAACAAGAGGTTGATCCTCGAAAATCTGCAGTTGATGAAGCTATTTCACGTGACAAAGGTCGGAAACTGGAACATCATCAGGCTAATGCGGAGCCAGAATAAGTGGTTGATCCTCGCAAAGCTGCCGTCGAAGc                                                   <  4:71215/151‑1 (MQ=255)
                                                                                             aGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCAAAAGCCCGAAAACCCGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTAGCTCCACCCAAAGCGGcagaagcacc                                                   >  4:998437/1‑142 (MQ=14)
                                                                                                cattagcgccgCCAGACCACCCGGGCGCTCCGCCCATCGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGc                                                <  5:947690/141‑1 (MQ=14)
                                                                                                 tAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCGGCCGTCGAAGCAGCTATTGCCCGAGCCAAAGCGAGAAAACGCGAACAGCAGCAGGATAAAGCGGAGCAAGAATAACATGTAGATACGCCAAAAGCAGCCTTTGAAGCgca                                                >  3:595399/1‑148 (MQ=9)
                                                                                                 tAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACAGGCTAATGAGGAGCCAGAAGAACAGCTAGATCCGCGCAAAGCTGCCGTAGAAGCGGCt                                               >  4:616839/1‑151 (MQ=255)
                                                                                                  aaTGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCACCCGGCTAATGCGGAGCCAGAAGACCAGGTTGATCCGCGCAAAGCTGCAGTCGCCGCGGCt                                               >  8:370745/1‑150 (MQ=17)
                                                                                                  aaTGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCAACAGGCTAATGAGGAGCCAGAAGAACAGGTTGATCAGCGCAACGCTGCAGTGGCCGCGGCt                                               >  1:1054396/1‑150 (MQ=14)
                                                                                                    tGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCAGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCACGCAAGCTAGAACAGAACACCGAAAATGAGGCAAAAGAAGAAGAGGATAATCAGCGAAAAGCTGcactcccagacaataa                                             >  1:884392/1‑135 (MQ=0)
                                                                                                      ccGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCAGTAGAAGCCGCTATTGCCCGAGCAAAAGCCAGAAAACGCGAACAGCAAAAGGATAATGAGGAGAAACAAGAACAGGATGATAAGCGCAAAGCTGCAGTAGAAGCTGCTATTGc                                          >  2:689654/1‑151 (MQ=9)
                                                                                                         aGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGACGCCGCTATTGCCCGCGCCAAAGCCCGCAAACGGGAACGGCAACAGGCTAATGCGGATCCAGAAGACGAGGTTGATCCGCGCAAAGCTGCCGTCGCCGCGGCTATTGCAcc                                       >  8:902852/1‑150 (MQ=14)
                                                                                                            cAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACgcgc                                    >  1:527995/1‑151 (MQ=255)
                                                                                                            cAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGACCAGCAACCGGCTAATGCGGAGCCAGAAGACCAGGTTGATCCGCGCAAAGCTGCCGTGGACGCGGCTATTGCACgcgc                                    >  7:420292/1‑151 (MQ=255)
                                                                                                             agaagaACAGGTCGATCCGCGCAAAGCCGCCGTAGAAGCCGCTATTGCCCGAGCCAAAGCCCGAAACCACGAACAGCAACAAGATAATCAGGACCCAGAAGAACAGGTGGATCAGCGCAAAGCGGAAGTAGACGCGGCTAttcccagcgc                                    >  3:330939/1‑142 (MQ=2)
                                                                                                              gAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGAGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTCGAAGCGGCTATTGCACGCGcc                                   >  6:642021/1‑150 (MQ=255)
                                                                                                               aagaacaGGTCGATCCGCGCAAAGCCGCAGTAGAAGCCGCTATTGCCCGCGCGAAAGCAAGCAACCGGGAACAGCAACAAGCTAATTCGGGCCCCGAAGAACAGGTAGATCCGCGCAAAGCTGCAGTAGACGCGGCTATTGCACtcgcta                                  >  1:1341761/4‑148 (MQ=2)
                                                                                                               aacaacAGGTCGATCCGCGCAAAGCCGCCGTGGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCTTAGAAGCGGCTATTGCACGCGCCa                                  >  6:306399/1‑150 (MQ=255)
                                                                                                               aacaacAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTGGCAGCGGCTATTGCACGCGCCa                                  >  7:710004/1‑150 (MQ=255)
                                                                                                                acaacaGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTGCCAGCGGCTATTGCACgcgcaa                                  >  3:979543/1‑147 (MQ=17)
                                                                                                                 caacaGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTAGACGCGGCTATTGCCCGCGCaaacg                               >  6:1450616/1‑149 (MQ=14)
                                                                                                                  aacaGGTCGATCCGCGCAAAGCCGCAGTCGAAGCCGCTATTGCCCGCGCCAAAGCCCGCAAACGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTAGAAGCGGCTATTGCAAgcgctcacgc                              >  3:839780/1‑145 (MQ=14)
                                                                                                                    caGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCCCGCGCCAAGGccc                            >  6:97179/1‑149 (MQ=255)
                                                                                                                        gcgacccccccAAACCCCCCGGCGAAGCCGCTATTGCCCTAGCCAAAGCGCGCAAACGGGAACAGCAACAGGCTAATGCGGAGCGACAAGAAGAGCTGTAGCCGCGAAAAGCCGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCaa                        <  8:1523343/141‑1 (MQ=14)
                                                                                                                         cGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGCCGCGGCTATTGCCCGCGCCAACGCACCCaac                       >  1:538731/1‑150 (MQ=255)
                                                                                                                          gATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAACCGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGACGCGGCTATTGCACGCGCCAACGCACGCAAGc                      >  7:1470535/1‑151 (MQ=255)
                                                                                                                           atcgccgcACATCCGCTCTCGAAGCAGCTATCGCCCGAGCCAAATCTCGCAACCGGGAACAGCAACAGGCTAATGCGGATCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCt                     <  2:1520504/146‑1 (MQ=14)
                                                                                                                           aTCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTAGAAGCGGCTATTGCACGCGCCCACGCaagcaagcg                     >  6:348665/1‑150 (MQ=17)
                                                                                                                            tCCGCGCAACCCCGCCGGCGAAGCCGCTATTGCCAGTCCGCACGCGCGCAAACCGGTAGCCGATCCGGCTAATGCGGACCCAGAACAACAGGTCGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCt                     <  8:887309/150‑1 (MQ=14)
                                                                                                                              cccgcccaGCCGCCGTCGAAGCCGCTATGGCCCGAGCCAAAGCGCGCAACCGGGAACAGCAACAGGCTAATGCGGACCCAGAAGAACACGGTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGa                  <  6:756197/144‑1 (MQ=14)
                                                                                                                               ccgccaccccgccgGCGCAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACTGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGaa                 <  7:220417/143‑1 (MQ=17)
                                                                                                                                 cgcaccccccctccGAAGCCGCTATCGCCCGCGCCAAAGCGCGCAATCTGGAACACCAACAGGCTAATGCGGATGCAGAAGAACAGGTGTTTCCGCGCATATCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACa               <  7:494855/138‑1 (MQ=11)
                                                                                                                                   aaaGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCCCGCGCCAAAGCACGCAAGCTGGACCAGc             >  8:866638/1‑151 (MQ=255)
                                                                                                                                   aaaGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACATCAACAGCAACACGATAATGCGGAGCCAGAAGAAAATGTTGATCCGCGCAAAGCAGCCGTCGAAGAGGATATTGAACGAGCCAAAGCCCGCAAggagcggcagc             >  1:1518462/1‑141 (MQ=14)
                                                                                                                                     cgccgccGTCGAAGCCGCTATTGCCCGCGCCAAAGCCCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGGTGATCCGCGCAAAGCTGCCGTCGAAGAGGCTATTGCCCGCGCCAACGCCCGCAACCGGGCCCAGCa            >  8:399405/2‑150 (MQ=14)
                                                                                                                                       ctgccgTTGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATg                                                                                                 <  1:77795/61‑1 (MQ=255)
                                                                                                                                       ccgccgTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAACGCACGCAAGCTGGCACAGCAAc          >  5:1164747/1‑150 (MQ=255)
                                                                                                                                       ccgccgTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAAc          >  2:1243191/1‑150 (MQ=255)
                                                                                                                                         gccgTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAgg       >  7:1380293/1‑151 (MQ=255)
                                                                                                                                          ccgTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGACGCGGCTATTGCACGCGCGAACGCACGCAAGCTCGCCCAGCACCAGGc      >  5:1322404/1‑151 (MQ=17)
                                                                                                                                           cgTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGATCAGCAACAGGCt     >  6:24932/1‑151 (MQ=255)
                                                                                                                                             tCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGCCGCGGCTATTGCCCGCGCCCAAGCACGCAAGCTGGAACAGCAACAGGCTa  >  1:356320/1‑150 (MQ=255)
                                                                                                                                             tCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGCCGCGGCTATTGCACGCGCCAACGCACGCAAGCTGGACCAGCAGCAGGCtc    >  2:322782/1‑149 (MQ=21)
                                                                                                                                             tCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAGCAGGCTa    >  4:443781/1‑150 (MQ=255)
                                                                                                                                              cGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCa                                                                                                            <  1:1322772/45‑1 (MQ=255)
                                                                                                                                              cGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAACGCACGCAAGCTGGAACAGCACCAGGCTAAt  >  8:287914/1‑151 (MQ=255)
                                                                                                                                              |                                                                                                                                                      
CTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAAT  >  NC_000913/1708741‑1709033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: