Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,606,1760TA24.8% 212.2 / 36.4 114intergenic (‑11/+24)leuV/leuPtRNA‑Leu/tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base T (12/73);  new base A (0/28);  total (13/101)
Fisher's exact test for biased strand distribution p-value = 3.59e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

GAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG  >  NC_000913/4606028‑4606324
                                                                                                                                                     |                                                                                                                                                    
gAATCAGGGATGAAAAATCAAGGAAGAACCAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTAc                                                                                                                                                    <  3:382908/151‑1 (MQ=255)
gAATCAGGGATGAAAAATCAAGGAACAAACAACAAAGGATGAAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTAc                                                                                                                                                    <  5:1288234/151‑1 (MQ=255)
gAACCAGGCATTAAAAATCAAGGAAGAAACAACAAAGGAAGTAAAGATAATTGGGGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTa                                                                                                                                                     <  2:842109/150‑1 (MQ=255)
caaTCAGGGATTAAAAATCAAGGAACAAACAACAACGCAAGTAAAGATAATTGGGGCGCGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTa                                                                                                                                                     <  6:762949/149‑1 (MQ=18)
 aaTCAGGGATGAAAAATCAAGTAACAAACAAGAAACCAAGTAAATAAATTTGGGCCGAGGGGGGGGCCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACt                                                                                                                                                   <  6:727231/151‑1 (MQ=18)
 aaTCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCAGAGACA‑GATTACt                                                                                                                                                   >  7:634757/1‑151 (MQ=255)
  atccgcgATAAAAAATCACGCAACAAACAACAAAGCCATTACATATATTTGGGGCGAGGGGGGGCACTTCAACCCCCACGTCCGTAACGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACtt                                                                                                                                                  <  2:1409544/145‑1 (MQ=14)
    cAGGGATTAAAAATCAAGTAAGAAACAAGAAAGGAAGTAAAGATAATTTGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACttt                                                                                                                                                 <  1:1456979/150‑1 (MQ=255)
    cAGGGATTAAAAATCAAGGACCAAACATCAACGGAAGTAAACATAATTGGTGCGCGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACttt                                                                                                                                                 <  4:1386150/150‑1 (MQ=18)
    cAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACttt                                                                                                                                                 <  1:1435851/150‑1 (MQ=255)
    cAGGGATCAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGCACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACtttt                                                                                                                                                <  8:1463849/151‑1 (MQ=255)
     aGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACttttt                                                                                                                                               <  2:1456635/151‑1 (MQ=255)
      gggATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACtttttt                                                                                                                                              <  4:960554/151‑1 (MQ=255)
        gATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACACTCA‑GCTTACtttaat                                                                                                                                              >  7:344393/1‑146 (MQ=255)
           gAAAAATCAAGGAAGAAACACGAACGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGata                                                                                                                                         <  5:204265/151‑1 (MQ=255)
           caaaaaTCACGCAAGAAACAAGAAAGGAAGCAACCATATTTGGGGCGAGGGGGGGGCAGTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGata                                                                                                                                         <  1:353662/150‑1 (MQ=18)
           aaaaaaTCAAGCAACAAACAAGAAAGGAAGTAAAGATAATTGGGGCGCGGGGGGGGACTGGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGata                                                                                                                                         <  6:426242/150‑1 (MQ=255)
            aaaaaTCAAGTAATAAAAAAGAATGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGatat                                                                                                                                        <  1:691635/151‑1 (MQ=255)
             aaaaTCACGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGCGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATc                                                                                                                                       <  8:614117/151‑1 (MQ=255)
             aaaaTCAAGGAATAAACAAGAAAGTAAGAAAATATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATc                                                                                                                                       <  3:1546308/151‑1 (MQ=255)
             aaaaTCAAGGAAGAAACAAGAAAGGAATTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATc                                                                                                                                       <  3:1032885/151‑1 (MQ=255)
             aaaaTCAAGGAAGAAACAACAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATc                                                                                                                                       <  5:1275255/151‑1 (MQ=255)
              aaaacacgcaacaaaCAAGAAAGCAAGTAAAGATAATTGGGGGGCGGGGGGGGACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCg                                                                                                                                      <  6:1572975/139‑1 (MQ=21)
              aaaTCAAGGAAGAAACAAGAAAGGAATTAAAGATAATTGGGGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCg                                                                                                                                      <  1:722268/151‑1 (MQ=255)
               aaTCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTCA‑GCTCGCATTTTGTCATATCGc                                                                                                                                     >  7:605993/1‑151 (MQ=18)
               aaTCAAGCAACAAACAAGAACGCAAGAAAAGATAATTGGGGCGCGGGGGGGCACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGc                                                                                                                                     <  7:1362514/151‑1 (MQ=21)
                 acAAGGAAGAAACACGAAAGCAAGTAAAGATAATTGGGGCGCGGGGGGGCCCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCt                                                                                                                                   <  7:234124/150‑1 (MQ=21)
                 acAAGCAACAAACAAGACAGGAAGTAAACATAATTGGTGCGCGGGGGGGGACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCt                                                                                                                                   <  5:422711/150‑1 (MQ=21)
                  caagcaagAAACAAGAAAGGAAGTAAAGATAATTGGGGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTc                                                                                                                                  <  6:1178823/151‑1 (MQ=255)
                   aaGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCg                                                                                                                                 <  8:866743/151‑1 (MQ=255)
                   aaGGAAGAAACAAGAAACGAATAAAAGATACTTGGTGCGTGGGGGGGGACTTGAACCCCCTCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCCCCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTct                                                                                                                                 <  3:180098/151‑2 (MQ=21)
                    acgaacaaACAAGAAAGCACGAAAAGATAATTGGTGCCAGGGGGGGGACTTCAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGt                                                                                                                                <  4:449022/149‑1 (MQ=21)
                      gaacaaAAAACAACGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTCAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGttt                                                                                                                              <  5:336823/151‑1 (MQ=255)
                       aagacACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTCAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTg                                                                                                                             <  5:585157/151‑1 (MQ=255)
                       aacaaaCACGAAAGGAAGTAAACATAATGGGGGCGCGGGGGGGGACTTCAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTCTTGATATCGCCTCGTTTg                                                                                                                             <  7:1202346/148‑1 (MQ=21)
                       aacaaaCAACAAAGTAAGAAAACATAATTGGTGCGAGGGGGGGGACTTCAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTg                                                                                                                             <  2:859119/148‑1 (MQ=21)
                        acaaaaaaTAACGGAAGTAAATATATTTGGTGCGCGGGGGGCTAGTCCACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTgg                                                                                                                            <  8:1566498/149‑1 (MQ=18)
                         gaaACATGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGt                                                                                                                           <  6:103941/151‑1 (MQ=255)
                         caaaCAAGAACCGAAGTAAAGATAATTGGGGCGCGGGGGGGCACTTCACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGt                                                                                                                           <  4:826216/150‑1 (MQ=21)
                         caaaCAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGt                                                                                                                           <  6:98951/150‑1 (MQ=255)
                          aaaCAATATAGGATGTTAAGTTTTTTGGTGCGAGGGGGGTGAGTTGTACGCCGAGGTGCGTAAGGAGAGTATGTGGTGTAGGTAGCGCGTCTAGCAATTGCGCCAGCTTCGCTGAGTCA‑TGTTACTTTTTTTGATATCGCCTCGTTTGGTg                                                                                                                          <  1:1031400/151‑1 (MQ=255)
                          aaaCAATAACGTAATTAAAGTTTTTTGTGGCGTGGGGGGTGACTTCAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTg                                                                                                                          <  3:788669/151‑1 (MQ=18)
                          aaaCAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGCACACTAACACCTGAAGCTAGCGCGTCTACCAATTACGCCACCTGCGCAGAGTCA‑TCATACTTTTTTTCATATCTCCTCGTTTGGTg                                                                                                                          >  4:1160957/1‑151 (MQ=21)
                             ccacaaaGTAATTAAATATACTGCGTGCGTGGGGGGTCACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGa                                                                                                                       <  2:1362735/147‑1 (MQ=18)
                               atactggAAGTAAAAATAATTGGGGCGTGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgg                                                                                                                     <  4:479632/146‑1 (MQ=255)
                                caacggAAGAAACGATAATTGGGGCGAGGGGGGCGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAggg                                                                                                                    <  4:1197708/147‑1 (MQ=255)
                                aaaaGGAAGTAAAGATAATTGGGGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAggg                                                                                                                    <  5:739255/150‑1 (MQ=255)
                                 aacggAAGAAAAGATAATTGGGGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggg                                                                                                                   <  5:979253/148‑1 (MQ=255)
                                 aaaGGACTTAAAGATAATTGGTGGGAGGGGGTGGACTTGAACCCCCACGTCAGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGCTGCGAgggg                                                                                                                   <  8:648975/151‑1 (MQ=21)
                                 aaaGGAATTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAggg                                                                                                                    <  1:980275/150‑1 (MQ=255)
                                 aaaGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggg                                                                                                                   <  8:691185/151‑1 (MQ=255)
                                 aaaGGAAGTAAAGATAATTGGGGCGAGCGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGATGCTAGGGCGGCTACCAATTCCCCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggg                                                                                                                   <  5:1007578/151‑1 (MQ=25)
                                         tAAACATAATTGGTGCGCGGGGGGGCCCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGGCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACt                                                                                                            <  7:1260663/150‑1 (MQ=255)
                                          aatcttatttGGTGCGTGGGGGGTGACTTTCAGCCCCACGTCCGTAAGGTCACTAACACATGAAGCTTTCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTg                                                                                                          <  4:162178/143‑1 (MQ=21)
                                               aatttgcggccaGGGGGGGTCCTTCCACCCCCACGTCCCTAAGGACACTAACACCTGCAGCTAGCGCGTCTACCAATTCCGCCACCTACGCACACTCA‑TCTTACTATTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAAccc                                                                                                     <  8:1568932/140‑1 (MQ=21)
                                                  tttttgctacgggggccACTTCAACCACCACTTAAGTAATCACACTTACCCCTCAAGCTCGCGCTTCTAACAATTCCGGCATCAAAAAACATTCA‑TCTTACTTTATTTGATATACCCTACTTTGGTGCG‑CGGGGGGGACTTGAACCCCCa                                                                                                  <  4:959364/140‑1 (MQ=255)
                                                          cggggggggCCTGTACCCCCCACGTCCGATTTTTCACTACAACATGAAGCTCCCGCTGATAGGAATTCCGCCACCTTCCCACAGGCA‑ACTTACTTTTTTTGATTTCCCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTa                                                                                          <  8:1346728/150‑1 (MQ=12)
                                                           ggggggggACTTCAACCCCCACGTCCTTAAGTTCACTAACACCTGAAGCTAGCCCGTCAACCAATTCCGCCACCTTCCCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTaa                                                                                         <  5:1547236/151‑1 (MQ=25)
                                                           gggcggttccTTCAACCCCCACGTCCGTAAGTACACTCACACCTGAAGCTCCTCCGGCAACCAATTCCGCCACCTACCCACAGTCA‑CGTTACTTTTTTTGATCTCCCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTaa                                                                                         <  8:624795/142‑1 (MQ=21)
                                                            gggttggACTTTAACCCCCACGTCCTTAAGTAAACTAAAACCTGAAGCTAGAGCGGAAACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCCCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAg                                                                                        <  5:201991/146‑1 (MQ=25)
                                                            ggggtgtccTTCAACCCCCACGGCCTTTTTTACAGTAAATCCTGATGCTAGCGCGGATACCAATTCAGCCACCTTCCCACAGGCA‑CCTAACATTTTTTGATCTCGCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAg                                                                                        <  8:342304/143‑1 (MQ=16)
                                                            gcgcgggcctcccaCCCCCACGGCCCCATCGCCACTCCCACCTGATGCTCCCCAGTCAACCCAATCCGCCACCAACCCACACGCA‑CCTAACTATTTTTGAACTCCCCACCTTTGGTGCCAGGGGGGGGACTTGAACCCCCACGTCCGTAAg                                                                                        <  1:284816/138‑1 (MQ=2)
                                                                tttccTTCAACCCGCACGTCCTTATTTACACTAACTCATGAAGATACCCATTAAACCAATTCCGCCACCAAACAACATTCA‑TCTAACATTTATTGATCTCCCCTCCTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAac                                                                                     <  8:1084292/146‑1 (MQ=12)
                                                                gttactacaaCCCCCACGTGCGTATATACACTAAAACATGAAGCTTGCACGGCTACCAATTCCGGCACCTTCCCACATGCA‑TGTTACTTTTTTTGATATCGCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAaca                                                                                    <  1:304299/143‑1 (MQ=21)
                                                                 tgACTTCAACCCCCACGTCCGTTAGGACACTAACACCTGATGCTCCCGCTTCTACCAATTCCGCCAGCTTACCACAGGCA‑TCTAACTTTTTTTGATATCCCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAaca                                                                                    <  5:741201/149‑1 (MQ=25)
                                                                    atacaaCCCCCACGTCCTTAATTACACTAAAACCTGAAGATAATCATTAAATCAAATCCGCCACCTTCTAACAGTCA‑TCTAACTATTTTTGATATATCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                <  2:1323116/147‑1 (MQ=16)
                                                                      agAACCCCCCCGGCCCTAACTACACTAACACCTTACTCTCTCTCTTCAAACAATTCAGCCACCTTATAACATTCA‑CCAAACAATAATTTAACTCCCCTCCTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAaca                                                                              <  1:286923/150‑1 (MQ=2)
                                                                               aCGGCCGTAAGGACACTAACACCTGAAGCTAGCCCGTATACCAATTCCGCCACCTTCCAACAGTCA‑TCTAAATTTTTTTGATATCCCCTCTTTTGGGGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCt                                                                     <  7:1137471/151‑1 (MQ=25)
                                                                                        aggacagtccaacatGATGCTACCGAGTATACAAATTCCGCCACCTTACAACAGGCA‑TCATACTTTTTTTGATTTCCCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTc                                                             <  6:311287/136‑1 (MQ=17)
                                                                                         gtacagTAACACATGAAGATATCGCGTATACCAATTCCGCCACCTTAGCACAGGCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCt                                                            <  5:720019/148‑1 (MQ=25)
                                                                                         ggacattcccacCTGATCCTAGCGCTTATTCGAATTCCGCCACGAACCACCAGGCA‑TCAAACTTTTTTTGATGTCCCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCt                                                            <  6:189508/141‑1 (MQ=9)
                                                                                            cacTAACCCCTCACGCTAGCGGGTCTACCATTTCCGCCCCGTGCGACCCGTAA‑TCTAACTTTTTTTGAAATCCCGTGGTTTGGTGCGTGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa                                                        <  7:1164728/151‑1 (MQ=17)
                                                                                            cacTAACACCTGAAGATCGATCTTCTACGAATTCCGGCACCATCCAACATGCA‑ACTAACTTTTTTTGAAATAGCCTCCTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa                                                        <  8:1343553/151‑1 (MQ=17)
                                                                                                aatacCTGAAGCTCGCGATTATACAAATTCCGCCACGAAAAAACATTCA‑ATAAACTTTTTTTTATTTCCCCTCTTTTGGTGCGTGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                                                    <  7:823413/148‑1 (MQ=9)
                                                                                                aaCACCTGAAGCTCCCGCGGATACCAATTCCGCCACGTAACCACAGTCA‑TCTAACTTTTTTTGATATCCCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                                                    <  5:819876/151‑1 (MQ=21)
                                                                                                  aacattatgcttacgctTCTACAAATTCCGCCACTTTCCAACA‑TCATTGTAACTTTTTTTGATTTCCCCACTTTTGGTGGGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                  <  7:96842/138‑1 (MQ=9)
                                                                                                           cTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCGGAAGCTAGCGCGTCAGCCAAAACCGCccccaaac                                         >  5:1539911/1‑147 (MQ=25)
                                                                                                           atgcacattatACAAAATCCGGCAGCTACACACAGGCA‑TCTAACTTTTTTTGAATTCCCGTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                                         <  8:376360/141‑1 (MQ=14)
                                                                                                               ctcgtaaacaaaTTCCGCCACCTTCCCACAGTCA‑TCTAACTTTTTTTTATATCCCCTGTTTTGGGGCGTGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                                          <  8:1572606/136‑1 (MQ=17)
                                                                                                               cgcggatACCAATGCCGCCACCTACCCACAGTCA‑TCTAACTTTTTTTTATATCCCGTCTTTTGGGGCGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCa                                     <  7:1138158/145‑1 (MQ=17)
                                                                                                                  gtatagctaTTCCGCCTCCTAAGCACAGGCA‑ACAAACTTTTTTTGATATCCCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCACCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAg                                    <  7:573919/141‑1 (MQ=17)
                                                                                                                   tatACCAATGCCGCCACCAACCAACAGCCA‑TGAAAATTTTTTTGATTTCCCCTGTTTGGGGGGGGGGGGGGGGACTTCACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                  <  7:851514/148‑1 (MQ=9)
                                                                                                                   tCTACCTATTCGTCCATATTTTCACATTCA‑TCTAACTTTTTTTTATTTATAGTTTTTTGGTGGGTGGGGGGGGATTTTAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                  <  4:604061/150‑1 (MQ=2)
                                                                                                                     tACCAATTCCGCCACCTTCGCACAGTCA‑TATTACTTATTTTGATTTCGGAACCTTTGGTGCGAGGGGGGGGCCTTGAACCCGCACGTCAGTAAGTAAAAATCCACATACCGGTAGAGCCTCTACCAATTCCGACACCTACGctaccttcc                                >  5:58255/1‑142 (MQ=255)
                                                                                                                      tccAATGCCGCCACGTTACAACATGCA‑TCTTACATTTTTGTATTTAGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg                                <  6:541522/148‑1 (MQ=17)
                                                                                                                      acaaaTTCCGCCACCTTACCACAGGCA‑TCTAACTTTTTTTGATATCCCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                              <  8:702268/148‑1 (MQ=18)
                                                                                                                       cctaTTCCGCCACCTAATAACATGCA‑TCTTACTTTTTTTGATTTCGCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGc                             <  1:1564933/148‑1 (MQ=18)
                                                                                                                       ccAATTCCGCCACCTTCGCACAGTCA‑GCTTACTTTTAATGATATAGCTGGGTTTCGGGCCCGGGGGGGCACGAGAACCACCACCGCCGTAAGAACACTAACACCTGAAGCTAGCCCGTCTACCCATTCCGCCCCCTTCGCGCAgggggcgg                             >  7:65095/1‑144 (MQ=255)
                                                                                                                        cAATTCCGCCACCTTCGCACAGTCA‑GCTTACTTTTATTGATATCGCCTCGTTTGGGGCGAGGCGGGGGCCTTGAACCACCACGTCCGTAAGAACACTACCACCTGCCGCTAGCGCCTCTACCGAATCCGCCCCCTTCGcaaagccagaca                             >  8:1274103/1‑139 (MQ=255)
                                                                                                                        aaaTTCCGCCACCAAACAACATGCA‑ACTAACATTTATTGATTTACCCTCCTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCa                            <  7:1256961/150‑1 (MQ=14)
                                                                                                                          aTTCCGCCACCAACCCACATTCA‑ACAAACTTTTATTGAATTCCCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAat                          <  7:501701/151‑1 (MQ=17)
                                                                                                                                gcacaTTCCCCCAGTCA‑TGTAACTTTTTTTGATATACCTTCTTTGGGGCCTCGGGGGGGGACTTCAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc                    <  4:711260/150‑1 (MQ=17)
                                                                                                                                ccACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCACGGCGACCAATATaaa                     >  6:641201/1‑150 (MQ=255)
                                                                                                                                      acccacaGGCA‑TCTTACTTTTTTGGATATCGCGTTGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGtt              <  8:514223/148‑1 (MQ=255)
                                                                                                                                       aacacaGGCA‑TCAAACATTATTGGAAATCCCGTCTATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGttt             <  5:1201706/149‑1 (MQ=17)
                                                                                                                                        gCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGGGAGACAAATATAAAAGTGTtttt            >  6:1140102/1‑151 (MQ=18)
                                                                                                                                          atcattcttCTTACTTTTTTTGATTTATCGTTTTTTGGTGGGTGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGtttt            <  4:1152533/142‑1 (MQ=18)
                                                                                                                                           caGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCAAGTGAGACCAATATCAACGAGGTTTTTgc         >  5:215728/1‑150 (MQ=255)
                                                                                                                                               cA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGACGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCAAGGGGCAGCAATATCAACggcgttgtaggagcg     >  5:96262/1‑137 (MQ=18)
                                                                                                                                                   cTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCAGAGGGTGAGCTATTTAAAAGACGTTATTGGTGCGAgg  >  8:218034/1‑151 (MQ=17)
                                                                                                                                                     |                                                                                                                                                    
GAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG  >  NC_000913/4606028‑4606324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑