Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,606,1920GC37.4% 114.2 / 32.1 99intergenic (‑27/+8)leuV/leuPtRNA‑Leu/tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base G (14/43);  new base C (0/34);  total (15/84)
Fisher's exact test for biased strand distribution p-value = 8.07e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

AAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAAC  >  NC_000913/4606042‑4606338
                                                                                                                                                       |                                                                                                                                                  
aaaacacgcaacaaaCAAGAAAGCAAGTAAAGATAATTGGGGGGCGGGGGGGGACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCg                                                                                                                                                    <  6:1572975/139‑1 (MQ=21)
aaaTCAAGGAAGAAACAAGAAAGGAATTAAAGATAATTGGGGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCg                                                                                                                                                    <  1:722268/151‑1 (MQ=255)
 aaTCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTCA‑GCTCGCATTTTGTCATATCGc                                                                                                                                                   >  7:605993/1‑151 (MQ=18)
 aaTCAAGCAACAAACAAGAACGCAAGAAAAGATAATTGGGGCGCGGGGGGGCACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGc                                                                                                                                                   <  7:1362514/151‑1 (MQ=21)
   acAAGGAAGAAACACGAAAGCAAGTAAAGATAATTGGGGCGCGGGGGGGCCCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCt                                                                                                                                                 <  7:234124/150‑1 (MQ=21)
   acAAGCAACAAACAAGACAGGAAGTAAACATAATTGGTGCGCGGGGGGGGACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCt                                                                                                                                                 <  5:422711/150‑1 (MQ=21)
    caagcaagAAACAAGAAAGGAAGTAAAGATAATTGGGGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTc                                                                                                                                                <  6:1178823/151‑1 (MQ=255)
     aaGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCg                                                                                                                                               <  8:866743/151‑1 (MQ=255)
     aaGGAAGAAACAAGAAACGAATAAAAGATACTTGGTGCGTGGGGGGGGACTTGAACCCCCTCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCCCCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTct                                                                                                                                               <  3:180098/151‑2 (MQ=21)
      acgaacaaACAAGAAAGCACGAAAAGATAATTGGTGCCAGGGGGGGGACTTCAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGt                                                                                                                                              <  4:449022/149‑1 (MQ=21)
        gaacaaAAAACAACGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTCAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGttt                                                                                                                                            <  5:336823/151‑1 (MQ=255)
        cacgaaACAAGAACGGAATTAAAGATAATTGGTGCCAGGGGGGGGCCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGttt                                                                                                                                            <  7:1467925/148‑1 (MQ=255)
         aagacACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTCAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTg                                                                                                                                           <  5:585157/151‑1 (MQ=255)
         aagaaACAACAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTg                                                                                                                                           <  8:1396856/151‑1 (MQ=255)
         aacaaaCACGAAAGGAAGTAAACATAATGGGGGCGCGGGGGGGGACTTCAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTCTTGATATCGCCTCGTTTg                                                                                                                                           <  7:1202346/148‑1 (MQ=21)
         aacaaaCAACAAAGTAAGAAAACATAATTGGTGCGAGGGGGGGGACTTCAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTg                                                                                                                                           <  2:859119/148‑1 (MQ=21)
          acaaaaaaTAACGGAAGTAAATATATTTGGTGCGCGGGGGGCTAGTCCACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTgg                                                                                                                                          <  8:1566498/149‑1 (MQ=18)
           gaaACATGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGt                                                                                                                                         <  6:103941/151‑1 (MQ=255)
           caaaCAAGAACCGAAGTAAAGATAATTGGGGCGCGGGGGGGCACTTCACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGt                                                                                                                                         <  4:826216/150‑1 (MQ=21)
           caaaCAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGt                                                                                                                                         <  6:98951/150‑1 (MQ=255)
            aaaCAATATAGGATGTTAAGTTTTTTGGTGCGAGGGGGGTGAGTTGTACGCCGAGGTGCGTAAGGAGAGTATGTGGTGTAGGTAGCGCGTCTAGCAATTGCGCCAGCTTCGCTGAGTCA‑TGTTACTTTTTTTGATATCGCCTCGTTTGGTg                                                                                                                                        <  1:1031400/151‑1 (MQ=255)
            aaaCAATAACGTAATTAAAGTTTTTTGTGGCGTGGGGGGTGACTTCAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTg                                                                                                                                        <  3:788669/151‑1 (MQ=18)
            aaaCAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTg                                                                                                                                        <  1:60799/151‑1 (MQ=255)
            aaaCAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGCACACTAACACCTGAAGCTAGCGCGTCTACCAATTACGCCACCTGCGCAGAGTCA‑TCATACTTTTTTTCATATCTCCTCGTTTGGTg                                                                                                                                        >  4:1160957/1‑151 (MQ=21)
               ccacaaaGTAATTAAATATACTGCGTGCGTGGGGGGTCACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGa                                                                                                                                     <  2:1362735/147‑1 (MQ=18)
                 atactggAAGTAAAAATAATTGGGGCGTGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgg                                                                                                                                   <  4:479632/146‑1 (MQ=255)
                  caacggAAGAAACGATAATTGGGGCGAGGGGGGCGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAggg                                                                                                                                  <  4:1197708/147‑1 (MQ=255)
                  aaaaGGAAGTAAAGATAATTGGGGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAggg                                                                                                                                  <  5:739255/150‑1 (MQ=255)
                   aacggAAGTAAAGATAATGGGGGCGCGGGGGGGCACTTCAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggg                                                                                                                                 <  7:719960/148‑1 (MQ=25)
                   aacggAAGAAAAGATAATTGGGGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggg                                                                                                                                 <  5:979253/148‑1 (MQ=255)
                   aaaGGACTTAAAGATAATTGGTGGGAGGGGGTGGACTTGAACCCCCACGTCAGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGCTGCGAgggg                                                                                                                                 <  8:648975/151‑1 (MQ=21)
                   aaaGGAATTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAggg                                                                                                                                  <  1:980275/150‑1 (MQ=255)
                   aaaGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggg                                                                                                                                 <  8:691185/151‑1 (MQ=255)
                   aaaGGAAGTAAAGATAATTGGGGCGAGCGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGATGCTAGGGCGGCTACCAATTCCCCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggg                                                                                                                                 <  5:1007578/151‑1 (MQ=25)
                           tAAACATAATTGGTGCGCGGGGGGGCCCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGGCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACt                                                                                                                          <  7:1260663/150‑1 (MQ=255)
                            aatcttatttGGTGCGTGGGGGGTGACTTTCAGCCCCACGTCCGTAAGGTCACTAACACATGAAGCTTTCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTg                                                                                                                        <  4:162178/143‑1 (MQ=21)
                                 aatttgcggccaGGGGGGGTCCTTCCACCCCCACGTCCCTAAGGACACTAACACCTGCAGCTAGCGCGTCTACCAATTCCGCCACCTACGCACACTCA‑TCTTACTATTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAAccc                                                                                                                   <  8:1568932/140‑1 (MQ=21)
                                    tttttgctacgggggccACTTCAACCACCACTTAAGTAATCACACTTACCCCTCAAGCTCGCGCTTCTAACAATTCCGGCATCAAAAAACATTCA‑TCTTACTTTATTTGATATACCCTACTTTGGTGCG‑CGGGGGGGACTTGAACCCCCa                                                                                                                <  4:959364/140‑1 (MQ=255)
                                         gcctggcgtctgccTTCAACCGCCACGTCCCTATCTTCACTACCACCTGAAGCTACCGAGGCAACAAATTCCGCGACCTTCGCACAGTGA‑TCTAACTTTTTTTGATATCCCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTcc                                                                                                           <  2:146886/138‑1 (MQ=19)
                                            cggggggggCCTGTACCCCCCACGTCCGATTTTTCACTACAACATGAAGCTCCCGCTGATAGGAATTCCGCCACCTTCCCACAGGCA‑ACTTACTTTTTTTGATTTCCCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTa                                                                                                        <  8:1346728/150‑1 (MQ=12)
                                             ggggggggACTTCAACCCCCACGTCCTTAAGTTCACTAACACCTGAAGCTAGCCCGTCAACCAATTCCGCCACCTTCCCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTaa                                                                                                       <  5:1547236/151‑1 (MQ=25)
                                             gggcggttccTTCAACCCCCACGTCCGTAAGTACACTCACACCTGAAGCTCCTCCGGCAACCAATTCCGCCACCTACCCACAGTCA‑CGTTACTTTTTTTGATCTCCCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTaa                                                                                                       <  8:624795/142‑1 (MQ=21)
                                              gggttggACTTTAACCCCCACGTCCTTAAGTAAACTAAAACCTGAAGCTAGAGCGGAAACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCCCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAg                                                                                                      <  5:201991/146‑1 (MQ=25)
                                              ggggtgtccTTCAACCCCCACGGCCTTTTTTACAGTAAATCCTGATGCTAGCGCGGATACCAATTCAGCCACCTTCCCACAGGCA‑CCTAACATTTTTTGATCTCGCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAg                                                                                                      <  8:342304/143‑1 (MQ=16)
                                              gcgcgggcctcccaCCCCCACGGCCCCATCGCCACTCCCACCTGATGCTCCCCAGTCAACCCAATCCGCCACCAACCCACACGCA‑CCTAACTATTTTTGAACTCCCCACCTTTGGTGCCAGGGGGGGGACTTGAACCCCCACGTCCGTAAg                                                                                                      <  1:284816/138‑1 (MQ=2)
                                                  tttccTTCAACCCGCACGTCCTTATTTACACTAACTCATGAAGATACCCATTAAACCAATTCCGCCACCAAACAACATTCA‑TCTAACATTTATTGATCTCCCCTCCTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAac                                                                                                   <  8:1084292/146‑1 (MQ=12)
                                                  gttactacaaCCCCCACGTGCGTATATACACTAAAACATGAAGCTTGCACGGCTACCAATTCCGGCACCTTCCCACATGCA‑TGTTACTTTTTTTGATATCGCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAaca                                                                                                  <  1:304299/143‑1 (MQ=21)
                                                   tgACTTCAACCCCCACGTCCGTTAGGACACTAACACCTGATGCTCCCGCTTCTACCAATTCCGCCAGCTTACCACAGGCA‑TCTAACTTTTTTTGATATCCCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAaca                                                                                                  <  5:741201/149‑1 (MQ=25)
                                                      atacaaCCCCCACGTCCTTAATTACACTAAAACCTGAAGATAATCATTAAATCAAATCCGCCACCTTCTAACAGTCA‑TCTAACTATTTTTGATATATCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                              <  2:1323116/147‑1 (MQ=16)
                                                        agAACCCCCCCGGCCCTAACTACACTAACACCTTACTCTCTCTCTTCAAACAATTCAGCCACCTTATAACATTCA‑CCAAACAATAATTTAACTCCCCTCCTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAaca                                                                                            <  1:286923/150‑1 (MQ=2)
                                                                 aCGGCCGTAAGGACACTAACACCTGAAGCTAGCCCGTATACCAATTCCGCCACCTTCCAACAGTCA‑TCTAAATTTTTTTGATATCCCCTCTTTTGGGGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCt                                                                                   <  7:1137471/151‑1 (MQ=25)
                                                                        tatttacacTAACACATGAAGATAAACCGTATATCAAATCCGGCACCAAAAAACAGTCA‑TCTAACATTTTTTGATATCCCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcgc                                                                              <  5:775458/144‑1 (MQ=12)
                                                                          aggacagtccaacatGATGCTACCGAGTATACAAATTCCGCCACCTTACAACAGGCA‑TCATACTTTTTTTGATTTCCCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTc                                                                           <  6:311287/136‑1 (MQ=17)
                                                                          aGGACACTAAAACATGCAGCTCCCGCGTATACCAATTCCGCCACCTTCCCACAGTCA‑TCTTACTTTTTTTGATATCCCGTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCt                                                                          <  7:1074905/151‑1 (MQ=21)
                                                                           gtacagTAACACATGAAGATATCGCGTATACCAATTCCGCCACCTTAGCACAGGCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCt                                                                          <  5:720019/148‑1 (MQ=25)
                                                                           ggacattcccacCTGATCCTAGCGCTTATTCGAATTCCGCCACGAACCACCAGGCA‑TCAAACTTTTTTTGATGTCCCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCt                                                                          <  6:189508/141‑1 (MQ=9)
                                                                              cacTAACCCCTCACGCTAGCGGGTCTACCATTTCCGCCCCGTGCGACCCGTAA‑TCTAACTTTTTTTGAAATCCCGTGGTTTGGTGCGTGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                      <  7:1164728/151‑1 (MQ=17)
                                                                              cacTAACACCTGAAGATCGATCTTCTACGAATTCCGGCACCATCCAACATGCA‑ACTAACTTTTTTTGAAATAGCCTCCTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                      <  8:1343553/151‑1 (MQ=17)
                                                                                  aatacCTGAAGCTCGCGATTATACAAATTCCGCCACGAAAAAACATTCA‑ATAAACTTTTTTTTATTTCCCCTCTTTTGGTGCGTGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                                                                  <  7:823413/148‑1 (MQ=9)
                                                                                  aaCACCTGAAGCTCCCGCGGATACCAATTCCGCCACGTAACCACAGTCA‑TCTAACTTTTTTTGATATCCCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                                                                  <  5:819876/151‑1 (MQ=21)
                                                                                   aaacCTGCAGCTACCGCGTAATACAATTCCGCCACCTTCCCCAACGCA‑CAAAAACTTTTTTGATATCCCCCTCTTTGGTGCGCGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                                                 <  8:470755/149‑1 (MQ=2)
                                                                                    aacattatgcttacgctTCTACAAATTCCGCCACTTTCCAACA‑TCATTGTAACTTTTTTTGATTTCCCCACTTTTGGTGGGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                <  7:96842/138‑1 (MQ=9)
                                                                                             cTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCGGAAGCTAGCGCGTCAGCCAAAACCGCccccaaac                                                       >  5:1539911/1‑147 (MQ=25)
                                                                                             atgcacattatACAAAATCCGGCAGCTACACACAGGCA‑TCTAACTTTTTTTGAATTCCCGTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                                                       <  8:376360/141‑1 (MQ=14)
                                                                                                 ctcgtaaacaaaTTCCGCCACCTTCCCACAGTCA‑TCTAACTTTTTTTTATATCCCCTGTTTTGGGGCGTGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                                                        <  8:1572606/136‑1 (MQ=17)
                                                                                                 cgcggatACCAATGCCGCCACCTACCCACAGTCA‑TCTAACTTTTTTTTATATCCCGTCTTTTGGGGCGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCa                                                   <  7:1138158/145‑1 (MQ=17)
                                                                                                    gtatagctaTTCCGCCTCCTAAGCACAGGCA‑ACAAACTTTTTTTGATATCCCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCACCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAg                                                  <  7:573919/141‑1 (MQ=17)
                                                                                                     tatACCAATGCCGCCACCAACCAACAGCCA‑TGAAAATTTTTTTGATTTCCCCTGTTTGGGGGGGGGGGGGGGGACTTCACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                                <  7:851514/148‑1 (MQ=9)
                                                                                                     tCTACCTATTCGTCCATATTTTCACATTCA‑TCTAACTTTTTTTTATTTATAGTTTTTTGGTGGGTGGGGGGGGATTTTAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                                <  4:604061/150‑1 (MQ=2)
                                                                                                       tACCAATTCCGCCACCTTCGCACAGTCA‑TATTACTTATTTTGATTTCGGAACCTTTGGTGCGAGGGGGGGGCCTTGAACCCGCACGTCAGTAAGTAAAAATCCACATACCGGTAGAGCCTCTACCAATTCCGACACCTACGctaccttcc                                              >  5:58255/1‑142 (MQ=255)
                                                                                                        tccAATGCCGCCACGTTACAACATGCA‑TCTTACATTTTTGTATTTAGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg                                              <  6:541522/148‑1 (MQ=17)
                                                                                                        acaaaTTCCGCCACCTTACCACAGGCA‑TCTAACTTTTTTTGATATCCCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                            <  8:702268/148‑1 (MQ=18)
                                                                                                         cctaTTCCGCCACCTAATAACATGCA‑TCTTACTTTTTTTGATTTCGCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGc                                           <  1:1564933/148‑1 (MQ=18)
                                                                                                         ccAATTCCGCCACCTTCGCACAGTCA‑GCTTACTTTTAATGATATAGCTGGGTTTCGGGCCCGGGGGGGCACGAGAACCACCACCGCCGTAAGAACACTAACACCTGAAGCTAGCCCGTCTACCCATTCCGCCCCCTTCGCGCAgggggcgg                                           >  7:65095/1‑144 (MQ=255)
                                                                                                          cAATTCCGCCACCTTCGCACAGTCA‑GCTTACTTTTATTGATATCGCCTCGTTTGGGGCGAGGCGGGGGCCTTGAACCACCACGTCCGTAAGAACACTACCACCTGCCGCTAGCGCCTCTACCGAATCCGCCCCCTTCGcaaagccagaca                                           >  8:1274103/1‑139 (MQ=255)
                                                                                                          aaaTTCCGCCACCAAACAACATGCA‑ACTAACATTTATTGATTTACCCTCCTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCa                                          <  7:1256961/150‑1 (MQ=14)
                                                                                                            aTTCCGCCACCAACCCACATTCA‑ACAAACTTTTATTGAATTCCCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAat                                        <  7:501701/151‑1 (MQ=17)
                                                                                                                  gcacaTTCCCCCAGTCA‑TGTAACTTTTTTTGATATACCTTCTTTGGGGCCTCGGGGGGGGACTTCAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc                                  <  4:711260/150‑1 (MQ=17)
                                                                                                                  ccACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCACGGCGACCAATATaaa                                   >  6:641201/1‑150 (MQ=255)
                                                                                                                       atatctcaGTGA‑TCTTACTTTTTTTGATTTCCCCTTATTTGGTGGGTGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTgg                              <  4:562620/144‑1 (MQ=18)
                                                                                                                       aaaacacaCTCA‑TCTAACAATTTTTGAACTCCCCTCCTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGt                             <  5:28957/147‑1 (MQ=18)
                                                                                                                        acccacaGGCA‑TCTTACTTTTTTGGATATCGCGTTGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGtt                            <  8:514223/148‑1 (MQ=255)
                                                                                                                         aacacaGGCA‑TCAAACATTATTGGAAATCCCGTCTATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGttt                           <  5:1201706/149‑1 (MQ=17)
                                                                                                                          gCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGGGAGACAAATATAAAAGTGTtttt                          >  6:1140102/1‑151 (MQ=18)
                                                                                                                            cacatgcatcaaaCTTTATTTGAAATCGCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGttttt                         <  7:1045852/139‑1 (MQ=18)
                                                                                                                            atcattcttCTTACTTTTTTTGATTTATCGTTTTTTGGTGGGTGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGtttt                          <  4:1152533/142‑1 (MQ=18)
                                                                                                                             caGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCAAGTGAGACCAATATCAACGAGGTTTTTgc                       >  5:215728/1‑150 (MQ=255)
                                                                                                                             acatgcaccaaaCTTTTTTGGATGTCACCTGTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTg                        <  7:300118/140‑1 (MQ=18)
                                                                                                                              aattgatgtaaCTTTTTTTGATTTATTCTTTTTTTGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTTAGAAGACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTgg                       <  3:1362275/141‑1 (MQ=18)
                                                                                                                              aattcaacaaacattttttGATATATACTTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGTACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTgg                       <  4:443809/138‑1 (MQ=17)
                                                                                                                                tCA‑TGTTACTATTATTGATTTCTCTTAATTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTg                     <  5:1221528/150‑1 (MQ=18)
                                                                                                                                 cA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGACGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCAAGGGGCAGCAATATCAACggcgttgtaggagcg                   >  5:96262/1‑137 (MQ=18)
                                                                                                                                  gaacaaaCTTTATTGGATTTACCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGc                    <  3:31431/144‑1 (MQ=18)
                                                                                                                                   aacaaacattttttGAATTACCCTTGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa                  <  8:1377254/143‑1 (MQ=18)
                                                                                                                                     cTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCAGAGGGTGAGCTATTTAAAAGACGTTATTGGTGCGAgg                >  8:218034/1‑151 (MQ=17)
                                                                                                                                       aagttttttgGATTTACCGTCATTTGGGGCGTGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTATCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa                  <  8:378660/144‑1 (MQ=18)
                                                                                                                                          tttttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCAAGACACACGAATAACAACGAGGTGGTAGCTGCGAggcgggg           >  4:746553/1‑151 (MQ=14)
                                                                                                                                            tttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCCTAAGCACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGC‑‑CCGTCGCCCAGTGCGTGCAATATGAACGTGGTTTGTGGGCCGGGGGGGGCGagg       >  3:1331477/1‑149 (MQ=18)
                                                                                                                                                tGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAAGTGGTTTTTGGTGCGAGGGGGGGGACTTg     >  4:93763/1‑151 (MQ=255)
                                                                                                                                                   tatCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCGAGAGCGACCAATATCAAAGTGGTTTTTGGTGCGAGGCGGGGGCCTTGCAc  >  7:32546/1‑151 (MQ=18)
                                                                                                                                                       |                                                                                                                                                  
AAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA‑TCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAAC  >  NC_000913/4606042‑4606338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑