Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 962,358 G→C V122L (GTT→CTT)  rpsA → 30S ribosomal subunit protein S1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913962,3580GC94.0% 1464.3 / 60.8 401V122L (GTT→CTT) rpsA30S ribosomal subunit protein S1
Reads supporting (aligned to +/- strand):  ref base G (12/12);  new base C (179/197);  total (192/209)
Fisher's exact test for biased strand distribution p-value = 8.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

Only 100 of 401 total aligned reads displayed.

GGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTGTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTCGTC  >  NC_000913/962210‑962505
                                                                                                                                                    |                                                                                                                                                   
ggACGCAGTAGAAGACGGCTTCTGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAACGGCGGCATCACTCtt                                                                                                                                                   >  1:119117/1‑151 (MQ=255)
ggACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCtt                                                                                                                                                   <  1:981828/151‑1 (MQ=255)
   cGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCGAAGTTAAGGGCGGCTTCACTCTTGAg                                                                                                                                                <  8:1515160/151‑1 (MQ=255)
   cGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAg                                                                                                                                                <  3:914679/151‑1 (MQ=255)
     cAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCt                                                                                                                                              >  7:206969/1‑151 (MQ=255)
        tAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGa                                                                                                                                            >  2:1318739/1‑150 (MQ=255)
         agaagaCGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTAATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAAc                                                                                                                                          >  6:422249/1‑151 (MQ=255)
           aagaCGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGTAAAATGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTGACTCTTGAGCTGAACg                                                                                                                                         <  4:1300656/150‑1 (MQ=255)
           aagaCGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACgg                                                                                                                                        <  2:1106134/151‑1 (MQ=255)
            agaCGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTGTTGAGCTGAACGGt                                                                                                                                       <  8:164711/151‑1 (MQ=255)
              aCGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTAt                                                                                                                                     >  8:520180/1‑151 (MQ=255)
                ggCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTc                                                                                                                                   <  6:157481/151‑1 (MQ=255)
                 gCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCg                                                                                                                                  >  1:220902/1‑151 (MQ=255)
                    tCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGt                                                                                                                                 <  4:555107/149‑1 (MQ=255)
                    tCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGt                                                                                                                                 <  3:1345038/149‑1 (MQ=255)
                       gTGAAACTCTGCTGTCACGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTGTTGAGCTGAACGGTATTCGTGCGtt                                                                                                                            >  2:646923/1‑151 (MQ=255)
                          aaaCTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCt                                                                                                                         <  5:1283163/151‑1 (MQ=255)
                          aaaCTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCt                                                                                                                         <  1:1555406/151‑1 (MQ=255)
                          aaaCTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCt                                                                                                                         <  6:534189/151‑1 (MQ=255)
                          aaaCTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGATGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCt                                                                                                                         >  1:1059697/1‑151 (MQ=255)
                          aaaCTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATAACGAAGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGTGCGGCTTCACTCTTGAGCTGAACGTTAATCGTGCGTTCCt                                                                                                                         >  8:1295774/1‑151 (MQ=255)
                            aCTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGc                                                                                                                       >  8:280059/1‑151 (MQ=255)
                              tctGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTGTTGAGCTGAACGGTATTCGTGCGTTCCTGcc                                                                                                                      >  6:1019338/1‑150 (MQ=255)
                                tgctgTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAgg                                                                                                                   >  4:646857/1‑151 (MQ=255)
                                 gctgTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTGTTGAGCTGAACGGTATTCGTGCGTTCCTGCCa                                                                                                                     <  3:777769/148‑1 (MQ=255)
                                 gctgTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCa                                                                                                                     <  1:762211/148‑1 (MQ=255)
                                 gctgTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCa                                                                                                                     <  7:125049/148‑1 (MQ=255)
                                  ctgTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGtt                                                                                                                 >  6:775722/1‑151 (MQ=255)
                                    gTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTtct                                                                                                               <  3:1575649/151‑1 (MQ=255)
                                    gTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTtct                                                                                                               >  2:1237914/1‑151 (MQ=255)
                                      cccGTTAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTTGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTctct                                                                                                             <  7:278695/151‑1 (MQ=255)
                                      cccGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTctct                                                                                                             <  6:1439820/151‑1 (MQ=255)
                                        cGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTgg                                                                                                           >  6:1174194/1‑151 (MQ=255)
                                             gaAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGAc                                                                                                      >  4:1362937/1‑151 (MQ=255)
                                              aaaGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACg                                                                                                     <  3:1024780/151‑1 (MQ=255)
                                                aGCTAAACGTCACGAAGCCTGGATCACGCTTGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGtt                                                                                                   <  4:889439/151‑1 (MQ=255)
                                                aGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGtt                                                                                                   <  7:511492/151‑1 (MQ=255)
                                                 gCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTCCCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTc                                                                                                  <  1:408823/151‑1 (MQ=255)
                                                    aaaCGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGt                                                                                                <  7:620082/150‑1 (MQ=255)
                                                          cACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGc                                                                                         <  3:1480719/151‑1 (MQ=255)
                                                          cACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGc                                                                                         >  1:483552/1‑151 (MQ=255)
                                                           aCGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCg                                                                                        >  3:611331/1‑151 (MQ=255)
                                                           aCGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCg                                                                                        >  7:948689/1‑151 (MQ=255)
                                                            cGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTGTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGt                                                                                       <  6:106043/151‑1 (MQ=255)
                                                              aaGCCTGGATCACGCTGGAAAAATCTTACGAAGATGCTGAAACTGTTGCCGGTTTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTAGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGTTGAGTg                                                                                      >  2:428642/1‑150 (MQ=255)
                                                               aGCCTGGAGCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTGCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGac                                                                                    <  3:686672/151‑1 (MQ=255)
                                                                  cTGGATCACCCACGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGAGGCTTCACTCTTTAGCTCAACGGTATTCGTGCGATCCTGCCAGGATCTCTGGTAGACCTACGTCCGGTGCGTGacaat                                                                                 >  1:896670/1‑149 (MQ=255)
                                                                   tGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACActc                                                                                >  5:546450/1‑151 (MQ=255)
                                                                   tGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACActc                                                                                >  4:924251/1‑151 (MQ=255)
                                                                   tGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACActc                                                                                >  8:1215347/1‑151 (MQ=255)
                                                                    ggATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACActct                                                                               >  8:773378/1‑151 (MQ=255)
                                                                    ggATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACActct                                                                               >  8:324842/1‑151 (MQ=255)
                                                                    ggATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACActct                                                                               <  4:188337/151‑1 (MQ=255)
                                                                     gATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTg                                                                              >  1:1253156/1‑151 (MQ=255)
                                                                       tCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGc                                                                             >  2:883407/1‑150 (MQ=255)
                                                                        cACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTGTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCa                                                                            <  7:391337/150‑1 (MQ=255)
                                                                        cACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCa                                                                            <  3:205374/150‑1 (MQ=255)
                                                                         aCGCTGGAAAAAGCTTACGAAGATGATGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGCTGCGTTACACTCTGCAcc                                                                          >  1:663845/1‑151 (MQ=255)
                                                                           gCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCTAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTg                                                                        <  4:996240/151‑1 (MQ=255)
                                                                           gCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTg                                                                        >  3:1306384/1‑151 (MQ=255)
                                                                           gCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTg                                                                        <  6:1361911/151‑1 (MQ=255)
                                                                            cTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTgg                                                                       >  5:403872/1‑151 (MQ=255)
                                                                             tGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACCCTCTGCACCTgg                                                                       >  3:1202003/1‑150 (MQ=255)
                                                                             tGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTgg                                                                       >  4:4545/1‑150 (MQ=255)
                                                                             tGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTgg                                                                       >  5:1168473/1‑150 (MQ=255)
                                                                              ggAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGTTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGa                                                                      >  6:1206077/1‑150 (MQ=255)
                                                                              ggAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGa                                                                      >  2:243750/1‑150 (MQ=255)
                                                                                aaaaaGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAgg                                                                   <  7:771473/151‑1 (MQ=255)
                                                                                aaaaaGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAgg                                                                   >  1:835907/1‑151 (MQ=255)
                                                                                aaaaaGCTAACGAAGTTGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCGTTCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAgg                                                                   <  2:142533/151‑1 (MQ=255)
                                                                                  aaaGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGc                                                                  >  7:1505704/1‑150 (MQ=255)
                                                                                   aaGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCaa                                                                <  3:1396202/151‑1 (MQ=255)
                                                                                   aaGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCa                                                                 >  8:1096991/1‑150 (MQ=255)
                                                                                      cTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCaa                                                                >  1:1382101/1‑148 (MQ=255)
                                                                                        tACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGc                                                           >  8:451737/1‑151 (MQ=255)
                                                                                        tACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGc                                                           >  7:549687/1‑151 (MQ=255)
                                                                                         aCGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCt                                                          >  2:335027/1‑151 (MQ=255)
                                                                                           gAAGATGCTGAAACTGTTACCGGTGTTATCTACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTg                                                        <  2:1576567/151‑1 (MQ=255)
                                                                                            aaGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCATAGAGCTTg                                                        >  2:724833/1‑150 (MQ=255)
                                                                                            aaGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTg                                                        >  4:766272/1‑150 (MQ=255)
                                                                                                   tGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTGTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTaa                                                 >  6:1506230/1‑150 (MQ=255)
                                                                                                       aCTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCATGATTTTAGAGTa                                             >  4:1031200/1‑150 (MQ=255)
                                                                                                        cTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAAt                                           >  7:1108725/1‑151 (MQ=255)
                                                                                                          gTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCa                                         <  4:1149022/151‑1 (MQ=255)
                                                                                                          gTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCa                                         <  8:135416/151‑1 (MQ=255)
                                                                                                          gTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTACTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAAta                                          >  7:1379915/1‑149 (MQ=255)
                                                                                                               cGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTg                                    >  1:600/1‑151 (MQ=255)
                                                                                                                 gtgtTATCAACGGCAAAGTTAAGGGCGGCTTCACTGTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGa                                  <  5:861874/151‑1 (MQ=255)
                                                                                                                     tATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATc                                >  5:1559851/1‑149 (MQ=255)
                                                                                                                     tATCAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCa                               <  5:244469/150‑1 (MQ=255)
                                                                                                                        cAACGGCAAAGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGaa                            <  7:1044444/150‑1 (MQ=255)
                                                                                                                                aaGTTAAGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGcaaca                    >  5:793331/1‑150 (MQ=255)
                                                                                                                                     aaGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACgttg              >  6:878172/1‑151 (MQ=255)
                                                                                                                                      aGGGCGGCTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTCAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACgttgt             >  2:1152372/1‑151 (MQ=255)
                                                                                                                                        ggcggcTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACgttgttg           >  8:1151938/1‑151 (MQ=255)
                                                                                                                                            gcTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtc       <  2:509539/151‑1 (MQ=255)
                                                                                                                                             cTTCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtct      >  8:633015/1‑151 (MQ=255)
                                                                                                                                              ttCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtctg     >  2:785289/1‑150 (MQ=255)
                                                                                                                                               tCACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtctc     <  3:492076/150‑1 (MQ=255)
                                                                                                                                                cACTCTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCtcg    <  6:250661/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGACGCAGTAGAAGACGGCTTCGGTGAAACTCTGCTGTCCCGTGAGAAAGCTAAACGTCACGAAGCCTGGATCACGCTGGAAAAAGCTTACGAAGATGCTGAAACTGTTACCGGTGTTATCAACGGCAAAGTTAAGGGCGGCTTCACTGTTGAGCTGAACGGTATTCGTGCGTTCCTGCCAGGTTCTCTGGTAGACGTTCGTCCGGTGCGTGACACTCTGCACCTGGAAGGCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCTCGTC  >  NC_000913/962210‑962505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑