Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,151,759 G→A E49K (GAA→AAA)  acpP → acyl carrier protein (ACP)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,151,7590GA99.5% 1466.0 / ‑3.1 382E49K (GAA→AAA) acpPacyl carrier protein (ACP)
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (190/190);  total (192/190)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

Only 100 of 388 total aligned reads displayed.

GAGTATGAGCACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACT  >  NC_000913/1151611‑1151906
                                                                                                                                                    |                                                                                                                                                   
    aTGAGCACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGt                                                                                                                                               <  3:180648/151‑1 (MQ=255)
    aTGAGCACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGt                                                                                                                                               <  6:799491/151‑1 (MQ=255)
    aTGAGCACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGt                                                                                                                                               >  6:415686/1‑151 (MQ=255)
      gAGCACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGtt                                                                                                                                              <  4:1562038/150‑1 (MQ=255)
       atcACTATGTAAGACCGCGTTAAGAAAATTATCGGCGAAGAGGTGGGCGTTAAGCAGGAAGAAGTTAGCAACAATGATTCTTTCGTTGAAGACCTGGGCTCGGATTCTCTTGACACCGTTTAGCTGGTAATGGCTCTGGAAAATGAGTTTg                                                                                                                                            <  2:577735/149‑1 (MQ=255)
        gCACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGa                                                                                                                                           <  2:739402/151‑1 (MQ=255)
         cACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGAt                                                                                                                                          <  4:614211/151‑1 (MQ=255)
         cACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGAt                                                                                                                                          <  2:225096/151‑1 (MQ=255)
         cACTAGCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGAt                                                                                                                                          <  3:1441832/151‑1 (MQ=255)
          aCTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGAt                                                                                                                                          >  6:125478/1‑150 (MQ=255)
          aCTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGAt                                                                                                                                          >  1:134811/1‑150 (MQ=255)
          aCTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATa                                                                                                                                         <  3:508741/151‑1 (MQ=255)
          aCTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGATAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGAt                                                                                                                                          >  7:1404773/1‑150 (MQ=255)
           cTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAGGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATa                                                                                                                                         >  2:990487/1‑150 (MQ=255)
           cTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATAc                                                                                                                                        >  2:684258/1‑151 (MQ=255)
               cGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTg                                                                                                                                      >  4:1420232/1‑149 (MQ=255)
                gaagaaCGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTgaga                                                                                                                                   <  4:768109/151‑1 (MQ=255)
                 aagaaCGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTg                                                                                                                                      >  5:547355/1‑147 (MQ=255)
                 aagaaCGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGGTAACCAGGAAGAAGTTACGAACAAAGCTTCTGTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGATGAGCTGGTAATCGCTCTGGAAAAAGAGTTTGATACtc                                                                                                                                      >  8:632206/1‑146 (MQ=255)
                  agaaCGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGAtt                                                                                                                                 >  5:1560810/1‑151 (MQ=255)
                    aaCGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTcc                                                                                                                               <  6:468414/151‑1 (MQ=255)
                     aCGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCg                                                                                                                              <  7:758176/151‑1 (MQ=255)
                      cgcgTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCgg                                                                                                                             >  2:1483786/1‑151 (MQ=255)
                       gcgTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCgg                                                                                                                             >  5:1490092/1‑150 (MQ=255)
                       gcgTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGa                                                                                                                            <  1:804687/151‑1 (MQ=255)
                       gcgTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGa                                                                                                                            <  4:708451/151‑1 (MQ=255)
                        cgTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGAc                                                                                                                           <  1:943644/151‑1 (MQ=255)
                         gTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACg                                                                                                                          <  2:378974/151‑1 (MQ=255)
                         gTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACg                                                                                                                          >  5:757334/1‑151 (MQ=255)
                            aaGAAAATTATCGGCGAACATCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACgaa                                                                                                                        <  2:918522/150‑1 (MQ=255)
                             aGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGa                                                                                                                            >  7:88976/1‑145 (MQ=255)
                                aaaTTATCTGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGGTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGc                                                                                                                   <  4:1150449/151‑1 (MQ=255)
                                aaaTTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGc                                                                                                                   >  4:241068/1‑151 (MQ=255)
                                aaaTTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGc                                                                                                                   >  5:1126961/1‑151 (MQ=255)
                                  aTTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTg                                                                                                                 <  3:367292/151‑1 (MQ=255)
                                     aTCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGCACGAAGAAGCTg                                                                                                                 >  8:1008928/1‑148 (MQ=255)
                                       cGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGaaa                                                                                                            <  4:950846/151‑1 (MQ=255)
                                        ggCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGaaaa                                                                                                           <  1:762066/151‑1 (MQ=255)
                                          cGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAAt                                                                                                          >  8:125886/1‑150 (MQ=255)
                                            aaCAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGCCACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATcac                                                                                                       >  8:46047/1‑151 (MQ=255)
                                               aGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATcaccac                                                                                                    >  4:511967/1‑151 (MQ=255)
                                                   gggCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCg                                                                                                  <  7:1306173/149‑1 (MQ=255)
                                                   gggCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCg                                                                                                  <  2:33793/149‑1 (MQ=255)
                                                   gggCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGtt                                                                                                >  8:1554455/1‑151 (MQ=255)
                                                     gctttAAGCAGGATGAAGTTACCAACTATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTGTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCAGCACCGTTCa                                                                                              <  2:1116740/148‑1 (MQ=255)
                                                      cGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAg                                                                                             <  6:923712/151‑1 (MQ=255)
                                                       gTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAgg                                                                                            >  6:1412395/1‑151 (MQ=255)
                                                          aaGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGTAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCt                                                                                          <  1:655045/150‑1 (MQ=255)
                                                          aaGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCt                                                                                          <  2:1581674/150‑1 (MQ=255)
                                                           aGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGc                                                                                        >  7:575659/1‑151 (MQ=255)
                                                              aGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCAt                                                                                     >  5:276348/1‑151 (MQ=255)
                                                                gaagaaGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTg                                                                                   <  3:1217764/151‑1 (MQ=255)
                                                                   gaagTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGAtt                                                                                <  1:1512029/151‑1 (MQ=255)
                                                                     agTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTAc                                                                              >  1:1455551/1‑151 (MQ=255)
                                                                     agTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTAc                                                                              >  2:1539599/1‑151 (MQ=255)
                                                                        tACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCACTTGACACCGTTGAGCAGGTAATGGCTCTGGAAAAAGACTTTGAAAATGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTAAGGAAGCCATTGATTACAt                                                                            >  2:1318696/1‑150 (MQ=255)
                                                                          ccAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCaa                                                                         <  4:874809/151‑1 (MQ=255)
                                                                            aacaaTGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACg                                                                       >  7:334401/1‑151 (MQ=255)
                                                                            aacaaTGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACg                                                                       <  1:1016333/151‑1 (MQ=255)
                                                                            aacaaTGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACg                                                                       >  4:1220630/1‑151 (MQ=255)
                                                                                    ttcttTCGTTGAAGCCCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGccacc                                                                 >  3:630933/1‑149 (MQ=255)
                                                                                    ttcttTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGccacc                                                                 >  1:396361/1‑149 (MQ=255)
                                                                                       tttCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCg                                                            >  4:370897/1‑151 (MQ=255)
                                                                                        ttCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGt                                                           >  3:98020/1‑151 (MQ=255)
                                                                                          cGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTa                                                          <  3:303708/150‑1 (MQ=255)
                                                                                          cGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTa                                                          >  2:456474/1‑150 (MQ=255)
                                                                                          cGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTa                                                          >  6:1459831/1‑150 (MQ=255)
                                                                                              gAAGAGCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTg                                                      >  4:1304845/1‑150 (MQ=255)
                                                                                              gAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTg                                                      >  1:855767/1‑150 (MQ=255)
                                                                                                  aCCTGGGCGCGTATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACAt                                                 <  7:17302/151‑1 (MQ=255)
                                                                                                  aCCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACAt                                                 <  6:1146123/151‑1 (MQ=255)
                                                                                                   ccTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACAtc                                                <  8:225916/151‑1 (MQ=255)
                                                                                                    cTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACAtct                                               >  6:1050806/1‑151 (MQ=255)
                                                                                                           cgGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCg                                        >  5:605935/1‑151 (MQ=255)
                                                                                                            ggATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCgg                                       >  6:594330/1‑151 (MQ=255)
                                                                                                             gATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGt                                      >  7:1531358/1‑151 (MQ=255)
                                                                                                              aTTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTc                                     <  7:1047531/151‑1 (MQ=255)
                                                                                                              aTTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTGTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTc                                     <  2:124460/151‑1 (MQ=255)
                                                                                                                tctcTTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGt                                   >  7:1213352/1‑151 (MQ=255)
                                                                                                                   ctTGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGtt                                  <  7:439141/149‑1 (MQ=255)
                                                                                                                     tGACACCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTc                                 <  2:665638/148‑1 (MQ=255)
                                                                                                                      gACAGCGTTGATGTGGTAATGTCTCTGTAAACAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCAGCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGGGAACATCTCGAGGCGGTCGTTCGAcc                             <  8:1164391/151‑1 (MQ=255)
                                                                                                                       acacCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGAcc                             <  2:818291/150‑1 (MQ=255)
                                                                                                                        cacCGTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACAACCGTTCAGGCTGCCATTGATTACATCAACGGCAACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGc                           >  6:1212133/1‑151 (MQ=255)
                                                                                                                            gTTGAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGa                       <  5:1398340/151‑1 (MQ=255)
                                                                                                                               gAGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGt                     <  5:661191/150‑1 (MQ=255)
                                                                                                                               gAGCTGGTAATGGCTCTGGAAAAAAAGTTTGATACTGAGATTCCGCACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGGCGTTCGACCGCCTGAGt                     <  1:2013/150‑1 (MQ=255)
                                                                                                                                aGCTTGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGttt                   <  1:604927/151‑1 (MQ=255)
                                                                                                                                aGCTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGttt                   <  1:1102549/151‑1 (MQ=255)
                                                                                                                                  cTGGTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGtttt                  >  8:360559/1‑150 (MQ=255)
                                                                                                                                    ggTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTAt                >  3:73443/1‑150 (MQ=255)
                                                                                                                                    ggTAATGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATc               <  6:29679/151‑1 (MQ=255)
                                                                                                                                        aTGGCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGTTCGTTCGACCGCCTGAGTTTTATCtttt           <  4:32487/151‑1 (MQ=255)
                                                                                                                                           gCTCTGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGt        >  5:1291037/1‑151 (MQ=255)
                                                                                                                                            ctctGGAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGCCCGCCTGAGTTTTATCTTTTTGTc       >  8:865749/1‑151 (MQ=255)
                                                                                                                                                ggAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCGGGATGCCATTGATTACATCAACGGCCACCGGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGCCCGCCTGCGTTTTAGCTTTTTGTCCCAc   >  5:947360/1‑151 (MQ=255)
                                                                                                                                                ggAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCa    <  6:1335476/150‑1 (MQ=255)
                                                                                                                                                ggAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCa    <  1:1279302/150‑1 (MQ=255)
                                                                                                                                                ggAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCAc   >  6:1544188/1‑151 (MQ=255)
                                                                                                                                                ggAAAAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCAc   >  8:1435051/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GAGTATGAGCACTATCGAAGAACGCGTTAAGAAAATTATCGGCGAACAGCTGGGCGTTAAGCAGGAAGAAGTTACCAACAATGCTTCTTTCGTTGAAGACCTGGGCGCGGATTCTCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACT  >  NC_000913/1151611‑1151906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: