BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,862,476	T→C	intergenic (‑47/‑295)	msrB ← / → gapA	methionine sulfoxide reductase B/glyceraldehyde‑3‑phosphate dehydrogenase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,862,476	0	T	C	94.5%	1512.3 / 47.6	436	intergenic (‑47/‑295)	msrB/gapA	methionine sulfoxide reductase B/glyceraldehyde‑3‑phosphate dehydrogenase A
Reads supporting (aligned to +/- strand): ref base T (13/11); new base C (227/184); total (240/196)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.13e-01

Only 100 of 436 total aligned reads displayed.

CGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCTAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAA  >  NC_000913/1862329‑1862623

 gACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTACAGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTATAATCGTGCTCACATTACGTCACTGATTCCAAc                                                                                                                                                  >  2:1004151/1‑150 (MQ=255)

   cccGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACaaa                                                                                                                                               <  4:1152074/151‑1 (MQ=255)

     cGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCTAACAAAAc                                                                                                                                             >  6:350473/1‑151 (MQ=255)

       tAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACAt                                                                                                                                           >  2:1511252/1‑151 (MQ=255)

        aaaTGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACAtt                                                                                                                                          >  1:398255/1‑151 (MQ=255)

            ggcggcTCTGTCCCATGATTCTGCGTCACGTAAACCTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAac                                                                                                                                       <  2:1463583/150‑1 (MQ=255)

            ggcggcTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAaca                                                                                                                                      <  1:23290/151‑1 (MQ=255)

            ggcggcTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAaca                                                                                                                                      <  8:766039/151‑1 (MQ=255)

            ggcggcTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAac                                                                                                                                       <  6:271999/150‑1 (MQ=255)

              cggcTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACAcc                                                                                                                                    >  4:954772/1‑151 (MQ=255)

              cggcTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACAcc                                                                                                                                    >  4:1019341/1‑151 (MQ=255)

                gcTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCa                                                                                                                                   >  6:992714/1‑150 (MQ=255)

                     gTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTGAGTTCTTGTGGCGAAGGTTTATTAGCCATTTGCTGACATCTCAGTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTg                                                                                                                               <  3:471295/149‑1 (MQ=255)

                         cATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCaaaa                                                                                                                         <  6:820433/151‑1 (MQ=255)

                         cATGATTCTGCGTCACGTAAAACTACATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCaaa                                                                                                                          >  1:88876/1‑150 (MQ=255)

                          aTGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAAt                                                                                                                        >  7:1391877/1‑151 (MQ=255)

                          aTGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAAt                                                                                                                        <  8:543666/151‑1 (MQ=255)

                             aTTCTGCGTCACGTATAACTGCATCTCGGACATATTTTTTTTCTGTTCTTCTGCAGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAAtttt                                                                                                                     <  3:874134/151‑1 (MQ=255)

                             aTTCTGCGTCACGTAAAACTGCATCTCGGACGAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAAtttt                                                                                                                     >  5:1256702/1‑151 (MQ=255)

                             aTTATGCGTCACGTAAAACTGCATCTAGTACAAATTTTTTTTCAGTTCTTCTTCAGAATGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGAAAAAtttt                                                                                                                     <  3:132394/151‑1 (MQ=255)

                                  gCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTc                                                                                                                  <  3:293575/149‑1 (MQ=255)

                                  gCGTCAAGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCTTGCTCACATTAAGTGACTGATTCCAACAAAACATTAAAACCAACTGGCAAAATTTTGTCCt                                                                                                                >  7:1042349/1‑151 (MQ=255)

                                     tCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTaa                                                                                                              >  7:1219029/1‑150 (MQ=255)

                                     tCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACAAACTGGCAAAATTTTGTCCTaa                                                                                                              >  8:1087921/1‑150 (MQ=255)

                                      cACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAAc                                                                                                            >  8:158293/1‑151 (MQ=255)

                                      cACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAAc                                                                                                            <  1:1478630/151‑1 (MQ=255)

                                      cACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAAc                                                                                                            <  8:664037/151‑1 (MQ=255)

                                       aCGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACt                                                                                                           >  5:1457323/1‑151 (MQ=255)

                                       aCGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACt                                                                                                           <  6:475786/151‑1 (MQ=255)

                                       aCGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACt                                                                                                           <  8:439043/151‑1 (MQ=255)

                                       aCGTAAAACTGCATCTCGGACAAATTTTTTTTCACTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCGAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACt                                                                                                           <  5:83157/151‑1 (MQ=255)

                                         gTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTGTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACttt                                                                                                         >  2:71017/1‑150 (MQ=255)

                                          tAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTg                                                                                                         >  4:445698/1‑150 (MQ=255)

                                           aaaaCTGCATCTCGGACAAATTTTTTTGCCGTCGTAGTGCGCAAGGTTATTTACCCATTTGCGCCCAAGGCCCTTAAATCGGGGTACCATTACTTGACGGATTCCACAAAAACATAACCACCACCTGCCAAATTTTTGGCGTAACCtgaat                                                                                                       >  3:1225649/1‑147 (MQ=255)

                                            aagcTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCTAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGAtc                                                                                                      <  6:1018720/148‑1 (MQ=255)

                                             aaCTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGAtct                                                                                                     >  2:840927/1‑151 (MQ=255)

                                                tGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTcg                                                                                                   >  3:404841/1‑150 (MQ=255)

                                                tGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTcg                                                                                                   >  7:469283/1‑150 (MQ=255)

                                                       cGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCTAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGaa                                                                                              <  2:192465/148‑1 (MQ=255)

                                                       cGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGaaa                                                                                             <  5:1401023/149‑1 (MQ=255)

                                                       cGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCACTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAAt                                                                                            <  1:1167505/150‑1 (MQ=255)

                                                           cAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTg                                                                                       >  3:165571/1‑151 (MQ=255)

                                                            aaaTTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGc                                                                                      >  6:1535375/1‑151 (MQ=255)

                                                              aTTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCAc                                                                                    >  5:707031/1‑151 (MQ=255)

                                                                   ttttCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGTCAAAATTTTGTCCTAAACTTGATCTCGACGAAATCGATGCACCTaa                                                                                >  1:1008888/1‑150 (MQ=255)

                                                                   tattCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTaa                                                                                <  5:1313416/148‑1 (MQ=255)

                                                                    tttCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTaaa                                                                               <  4:829192/150‑1 (MQ=255)

                                                                     ttCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAAt                                                                              >  1:1201989/1‑150 (MQ=255)

                                                                     ttCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAAt                                                                              <  4:569632/150‑1 (MQ=255)

                                                                     ttCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATc                                                                             >  5:380973/1‑151 (MQ=255)

                                                                      tCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATc                                                                             <  7:599366/150‑1 (MQ=255)

                                                                      tCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATc                                                                             <  7:368533/150‑1 (MQ=255)

                                                                       cAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGt                                                                           >  2:637345/1‑151 (MQ=255)

                                                                       cAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGt                                                                           >  4:765614/1‑151 (MQ=255)

                                                                          ttcttcTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGtgat                                                                        >  8:1001269/1‑151 (MQ=255)

                                                                               ctGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGaaa                                                                    >  3:576277/1‑150 (MQ=255)

                                                                                 gCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGaaa                                                                    <  1:325341/148‑1 (MQ=255)

                                                                                 gCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATc                                                                 >  7:1337357/1‑151 (MQ=255)

                                                                                      aGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACAtt                                                            >  4:296748/1‑151 (MQ=255)

                                                                                       ggTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACAttt                                                           <  8:327741/151‑1 (MQ=255)

                                                                                          ttattaGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACAttttt                                                         <  4:1442906/150‑1 (MQ=255)

                                                                                          ttattaGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACAttttt                                                         >  6:701697/1‑150 (MQ=255)

                                                                                          ttattaGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACAttttt                                                         >  7:681703/1‑150 (MQ=255)

                                                                                            attaTCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATc                                                      <  4:1286860/151‑1 (MQ=255)

                                                                                            attaGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATc                                                      >  6:520478/1‑151 (MQ=255)

                                                                                              taGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCg                                                     <  7:7385/150‑1 (MQ=255)

                                                                                                gCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTaa                                                  <  1:504586/151‑1 (MQ=255)

                                                                                                   aTTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTg                                               <  1:751161/151‑1 (MQ=255)

                                                                                                    tttGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACTTTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGc                                              >  6:934704/1‑151 (MQ=255)

                                                                                                      tGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATCGCTGCACCTAAATCGTGATGAAAATCACATATTTATCGTAATTGccc                                            >  2:947912/1‑151 (MQ=255)

                                                                                                        cTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCTAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCtt                                          >  6:1134399/1‑151 (MQ=255)

                                                                                                          cacaTCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCttt                                         <  6:1205801/150‑1 (MQ=255)

                                                                                                          cacaTCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTa                                        <  1:65562/151‑1 (MQ=255)

                                                                                                           acaTCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTaa                                       <  6:1296171/151‑1 (MQ=255)

                                                                                                            caTCTCACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTaaa                                      <  5:1596206/151‑1 (MQ=255)

                                                                                                                tcACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTc                                  >  5:596395/1‑151 (MQ=255)

                                                                                                                 cACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTaaaa                                     <  5:297418/147‑1 (MQ=255)

                                                                                                                 cACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAAtt                                   <  2:332686/149‑1 (MQ=255)

                                                                                                                 cACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAAtt                                   <  1:160971/149‑1 (MQ=255)

                                                                                                                 cACTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAAt                                    <  4:656231/148‑1 (MQ=255)

                                                                                                                  aCTTTAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATAGTAATTGCCCTTTAAAATTAgg                                >  4:1509061/1‑151 (MQ=255)

                                                                                                                   atttAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGAGATGAAAATAACATTTTTATCGTAATTGCCCTTTAAAATTCtgg                               >  5:1009176/2‑151 (MQ=255)

                                                                                                                      tAATCGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGgcg                            >  3:1085526/1‑151 (MQ=255)

                                                                                                                       aaTAGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTGTAAAATTCGGGgcgc                           >  3:64181/1‑151 (MQ=255)

                                                                                                                          cGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCg                         >  2:1140481/1‑150 (MQ=255)

                                                                                                                          cGTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTCCCCTTTAAAATTCGGGGCGCCg                         >  4:1353571/1‑150 (MQ=255)

                                                                                                                           gTGCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGAc                       <  6:608273/151‑1 (MQ=255)

                                                                                                                             gCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGAccc                     >  5:47572/1‑151 (MQ=255)

                                                                                                                             gCTCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGAccc                     >  5:1170685/1‑151 (MQ=255)

                                                                                                                               tCACATTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGAcccc                    >  3:1459329/1‑150 (MQ=255)

                                                                                                                                cacaTTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCAt                  <  7:535651/151‑1 (MQ=255)

                                                                                                                                cacaTTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCAt                  >  7:18374/1‑151 (MQ=255)

                                                                                                                                  caTTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCAtgt                <  8:269128/151‑1 (MQ=255)

                                                                                                                                   aTTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCAtgtg               <  4:865226/151‑1 (MQ=255)

                                                                                                                                   aTTACGTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCAtgtg               <  2:471580/151‑1 (MQ=255)

                                                                                                                                        gTGACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGtctc          <  8:1469874/151‑1 (MQ=255)

                                                                                                                                          gACTGATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCa         <  3:815982/150‑1 (MQ=255)

                                                                                                                                              gATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGccc    >  7:27733/1‑151 (MQ=255)

                                                                                                                                              gATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGccc    >  6:1405210/1‑151 (MQ=255)

                                                                                                                                              gATTCCAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGccc    >  8:869784/1‑151 (MQ=255)

CGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCTCACATCTCACTTTAATCGTGCTCACATTACGTGACTGATTCTAACAAAACATTAACACCAACTGGCAAAATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAA  >  NC_000913/1862329‑1862623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`