Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913422,4290TG27.7% 628.6 / 90.9 356intergenic (+70/‑89)proY/malZproline‑specific permease/maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (59/197);  new base G (97/1);  total (157/199)
Fisher's exact test for biased strand distribution p-value = 4.76e-44
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 373 total aligned reads displayed.

TTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCT  >  NC_000913/422281‑422579
                                                                                                                                                     |                                                                                                                                                      
    gcgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGtcgtcct                                                                                                                                                  >  2:221373/1‑151 (MQ=255)
      gTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGGCCCCGtccgccgcc                                                                                                                                                >  3:260291/1‑151 (MQ=255)
      gTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGtcctccgcc                                                                                                                                                >  5:514227/1‑151 (MQ=255)
      gTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGgcctccgcc                                                                                                                                                >  5:1659807/1‑151 (MQ=255)
       tGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGccc                                                                                                                                               >  4:1193930/1‑151 (MQ=255)
        ggATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTcccc                                                                                                                                              <  1:369835/151‑1 (MQ=255)
             gttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCaaa                                                                                                                                          >  8:1451875/1‑150 (MQ=255)
             gttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAat                                                                                                                                         <  2:1060560/151‑1 (MQ=255)
               tgtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAata                                                                                                                                        <  4:885545/150‑1 (MQ=255)
               tgtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAata                                                                                                                                        <  8:1618660/150‑1 (MQ=255)
                gggCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATc                                                                                                                                      <  1:1467789/149‑1 (MQ=255)
                 tgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCGCCCGTCCACCTCCCCCAAaaata                                                                                                                                      >  6:248972/1‑149 (MQ=255)
                  gCTGTTGATTGGCTGGTTGTTTAAACGCCGCCAGGATCGGCATCTGGCGGAAACCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATcc                                                                                                                                     <  6:1707394/150‑1 (MQ=255)
                    tGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCTCCTCCCCCAAAAATCCTTc                                                                                                                                  >  4:709841/1‑151 (MQ=255)
                    tGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCCActat                                                                                                                                       >  2:1647679/1‑146 (MQ=255)
                     gTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCtt                                                                                                                                   <  6:1513107/149‑1 (MQ=255)
                       tGATTGGGTGGATGTTAAAACGCCGCCACGATCGTCAGCTGTCTGAAAACCAGTAATCCTTGCGTTCTGTCAGCCGGTTCAGGAGGAAATCCTGA‑TCCTGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTGAg                                                                                                                                <  4:1377737/150‑1 (MQ=255)
                            ggCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgatgat                                                                                                                           <  8:1132820/150‑1 (MQ=255)
                            ggCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgatgat                                                                                                                           <  3:847574/150‑1 (MQ=255)
                              cTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCGCCCGTCCGCCGGCCCCCCATATCCGTCAGaggatg                                                                                                                          >  2:623019/1‑149 (MQ=255)
                                ggATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACGAGTAATGCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTg                                                                                                                      <  8:592920/151‑1 (MQ=255)
                                       tAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCCGCCTCCTCCCCCCAATATCCTTCACATGATGAGTGATCCtcc                                                                                                               >  7:65774/1‑149 (MQ=255)
                                        aaaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCGCCGTCCTCCTCCCCCCAATATCCTTCAGATGATGAGTGATCCTGcg                                                                                                              >  6:1012726/1‑150 (MQ=255)
                                           cgccgcCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCAAAAATCCTTCAGATGATGCGGGAGCCTGccgtt                                                                                                           >  7:375087/1‑147 (MQ=255)
                                             ccgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCGCCCCCCAATATCCTTCAGATGAGGCGTGTTCCGGCATTTgg                                                                                                         >  3:117576/1‑151 (MQ=255)
                                              cgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGGCCCCGGCGTCCGCCCCCCAAGATCCGTCAGAGGATGAGTGCTCCTGCATTCGGc                                                                                                        >  1:885922/1‑151 (MQ=255)
                                                  aCGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATg                                                                                                    <  7:783189/151‑1 (MQ=255)
                                                     aTCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCa                                                                                                 <  3:1666711/151‑1 (MQ=255)
                                                     aTCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGGCGGCCGCCCCCAAAGATCCGTCAGATGATGAGTGATCCTGCATGAGGCTCTgcg                                                                                                  >  7:302448/1‑148 (MQ=255)
                                                       cGTCAGCTGGCTGATAAGCAGTAATCCTTCCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TGCTGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGTCTATGGCAAg                                                                                               <  4:1238213/151‑1 (MQ=255)
                                                       cGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTGCTCCTCCCCCAAAAAACCTTCATATGATGATTGATCCGGGAGTAGGCTATGGCaac                                                                                               >  7:486795/1‑150 (MQ=255)
                                                       cGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCaa                                                                                                <  2:489756/150‑1 (MQ=255)
                                                        gTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAgg                                                                                              <  2:801551/151‑1 (MQ=255)
                                                          cAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTg                                                                                            <  7:956594/151‑1 (MQ=255)
                                                              gtggtgcaaaCCATTAAACCGTGCGTTCTTTAAGCCCGGTCACGTTGTAATCCCGATTCCGGTAATTCC‑AAAGGTTTCCCGCCCCGTCCCCCGCCCCCAAATAACCTTCACCTGCAGAGTGCTGCTGCATTAGGCTATGGCAAGGTGATCa                                                                                        <  1:1026850/144‑1 (MQ=255)
                                                               ggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAg                                                                                       <  4:369116/151‑1 (MQ=255)
                                                                  tgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCCAAAAACCGTCAGAGCATCAGTGATCGTGCATTAGCCGATGGCAAGGGGATCCGAtt                                                                                    >  1:58466/1‑151 (MQ=255)
                                                                   gaaatcctttAATCCTTGCGTTGTTTAAGCTTGTGCAGGAGGTATGTCTGA‑TGAGGCTTTTGCGAAACTTATCCGCACCCGGGCTTCTGCCCCATATATCCTTCAGATGATGAGTGATGCTTCATTAGGCTATTGCAAGGTGATCAGAttt                                                                                   <  6:347228/142‑1 (MQ=255)
                                                                   gAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAtt                                                                                    <  2:1233572/150‑1 (MQ=255)
                                                                   gAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAt                                                                                     <  4:321200/149‑1 (MQ=255)
                                                                     aaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtc                                                                                 <  8:388424/151‑1 (MQ=255)
                                                                     aaaCCAGTAATCCTTGCGCTCTGTAAGCCGGTTCAGGATGAAATCCTGA‑TCAGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCCGATTTtc                                                                                 <  2:976893/151‑1 (MQ=255)
                                                                      aaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtca                                                                                <  1:1360698/151‑1 (MQ=255)
                                                                         cAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCAGCGACCGTACGCCTCACCCAAATATCCTTCGTATTATGAGTGATCATGCAGTAGGCTATGGGAAGTTGTTCAGCTTTtgatg                                                                              >  8:289747/1‑149 (MQ=255)
                                                                             aaTCGTTTCGTTCTGTAAGCCGGTTCAGGAGGATATGCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCGCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGGATTCGGGTATTGCAAGGTGATCAGATTTTCATCACAgg                                                                         <  6:1222460/151‑1 (MQ=255)
                                                                             aaTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTCCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAg                                                                          <  5:603810/150‑1 (MQ=255)
                                                                                   tGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAAt                                                                    <  4:1096672/150‑1 (MQ=255)
                                                                                     cGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTat                                                                 <  4:527143/151‑1 (MQ=255)
                                                                                       ttCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGGCCCCGGCCTCCTCCCCCCAATATCCTTCAGATGGTGAGTGATGCTGGATTAGGCTATGGCTAGGTGGTCTGATTTTCATCACAGGGGaagtttgt                                                               >  3:107900/1‑145 (MQ=255)
                                                                                       ttCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCTTCAGCTGATGCGTGATGCTGCAGTAGGGTATGGCAAGGGGGTCCGATTTTGATCCCAGGGGCATTttgt                                                               >  5:395552/1‑150 (MQ=255)
                                                                                        tCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatga                                                               <  7:1333379/150‑1 (MQ=255)
                                                                                           gTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgatg                                                             <  6:1129115/149‑1 (MQ=255)
                                                                                           gTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGt                                                            <  5:1188219/150‑1 (MQ=255)
                                                                                           gTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGt                                                            <  7:933146/150‑1 (MQ=255)
                                                                                             aaGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCCATTCCGAAAGTTATCCGCCCCCGGGCGCGGGCCCCCAAGATCGTTCAGATGATGAGTGATCATGCATTAGGCTTTGGCAAGGTGATCAGATTTTCATAAGAGGGGAATTAATATGTTa                                                          >  1:1224191/1‑150 (MQ=255)
                                                                                              aGCCGGTTCATGTGGATATCCTGA‑TCCGGCTATTCCTAAAGTTATCCGCCCCCGTCCTCCTCCGCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTaaa                                                        <  3:1510925/151‑1 (MQ=255)
                                                                                                    ttCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGGGCTCCTCCCCCAAATATCCTTGAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTGTTCACAGGGGGATTTTGATGTTAAATGCATg                                                  >  4:1284393/1‑151 (MQ=255)
                                                                                                     tCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGGGCCGGGGGGCCGGGCCCAACTATCCTTCAGCGGATGCGTGCTGGTGGAGTTGGCTATGGCAAGGTGCTCAGAGTTTCATCACTGGGGAATTTTGATGTTAAATGGAttg                                                 >  5:1493559/1‑149 (MQ=255)
                                                                                                      cAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCACCCCCAAAAATCATTCAGATGATTAGTGATCCTGGATAAGGCGATGGCAAGGTGATCAGATTTTCATGAAAGGGGAATTATGATGTTTAATTAATGGc                                                >  3:366567/1‑151 (MQ=255)
                                                                                                       aggaggAAATCCTGC‑TCCGGCTATTCCGAAAGTTATCCGGCCCCGGCGGCCGCCCCCAAAAATCCGACAGAGGAGGAGTGCGAGGGCGATTGGGTTGGGGAAGGGGGTGAGATTTTCATCACAGCGGGTTTTTGATGTTAAATGCAGGGcc                                               >  2:1284857/1‑150 (MQ=255)
                                                                                                       aggaggAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCGTCCCCCAAATATCCTTCAGATGATGAGTGATCGTGCAGTAGGCTATGGGAAGGTGATGAGATTTTCATCACAGGGGAATTATGATGTTAAATTCATGGc                                                >  1:22940/1‑150 (MQ=255)
                                                                                                        ggaggaAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCCAATATCCTTCAGATGATGAGTGATGCTGCTTTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTTAATTCATTGccc                                              >  5:676296/1‑149 (MQ=255)
                                                                                                           ggaAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCt                                            <  6:490535/150‑1 (MQ=255)
                                                                                                           ggaAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTg                                           <  7:1530594/151‑1 (MQ=255)
                                                                                                           ggaAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAATATCCTTCAGATGATGAGGGATCGTGCATTAGGGTATGGGAAGGTGATCAGATTTTCATAAAAGGGGATTTATGATGTTAAATGAATTGCAccgg                                           >  8:806762/1‑149 (MQ=255)
                                                                                                             aaaTCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGcc                                         <  3:704871/151‑1 (MQ=255)
                                                                                                             aaaTCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGcc                                         <  3:1422114/151‑1 (MQ=255)
                                                                                                               aTCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCCAAGAAACTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCAAAGGGGAATTTTTATTTTAAATGAATGTCACCTGCCgg                                       >  8:1294416/1‑151 (MQ=255)
                                                                                                                 cgtGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCTTCCCCCAAATATCTTTCAGATGATGTGTGATCCTGCATTAGGCTATGGCAAGGTGATCTGATTTTCATCACAGGGTAATTATGATGTTAAATGCATGGCACCTGCCGGTg                                     <  4:631166/149‑1 (MQ=255)
                                                                                                                 ccTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGt                                      <  5:11507/150‑1 (MQ=255)
                                                                                                                     a‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCCAAAAACCTTAAGATGATGTGTGATCCTGCAGTAGGCTATGGCAAGGGGATCAGATTTTCTTAAAAGGCGCTAAATGATGTTAAATGCATGGCACCTGCCGGTGcccc                                 >  1:1327300/1‑151 (MQ=255)
                                                                                                                        ccGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCa                               <  8:206133/151‑1 (MQ=255)
                                                                                                                        ccGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCATGAAGATGATCAGTGATGCGCCAATAGGCTATGGGAACGTGATAAGAGTTTCATAACAGGGGAAATATGATGTTAAATGCATGGCACCTGCCGGGGccccc                                >  3:1055550/1‑150 (MQ=255)
                                                                                                                         cGGCTATTCCGAAAGTTATCCGCCCCCCGCCTCCTGCCCCAAAGATCCATCAGATGATGAGTGATGCTGCATTAGGCTATTGCAAGGTTATCAGGTTTTCATCACAGGGGAATTATTTTGTTTAATGAATGTCACCAGCCCGTGCCCCCa                               >  8:965481/1‑150 (MQ=255)
                                                                                                                            cTATTCCGAAAGTTATCCGCCCCCGGCGGCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGGATTAGGCTATGGCAAGGTGATCAGAGTTTCATGACAGGGGAATTATGATGTTAAATGCATGGCACCTGGCGGGGCCCCCCttgg                           >  4:180231/1‑149 (MQ=255)
                                                                                                                              aTTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTTAATGCATGGCACCTGCCGGTGCCCCCATTTg                           <  4:1546515/149‑1 (MQ=255)
                                                                                                                               ttCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCTTAAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTAATAACAGGGGAATTATGATGTTAAATGAATGGCACCTGCCGGGGCCCCCCgttgttt                        >  6:693988/1‑150 (MQ=255)
                                                                                                                                tCCGAAAGTTATCCGCGCCCGGCCGCCGCCCCCACATAGCCAACAGATGATGAGTGCTCCTGCAGTAGGGGATGGCAAGGTGCTCAGCTTTTGATGAGAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCGCCCAGTAGTTa                        >  2:1430944/1‑150 (MQ=255)
                                                                                                                                tCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTa                        <  6:8645/150‑1 (MQ=255)
                                                                                                                                 ccGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTaaa                      <  3:1276379/151‑1 (MQ=255)
                                                                                                                                  cGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATACTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGGGATCAGATTTTAATAACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCCGTGCCCCCATTTGTTaaa                      >  8:1025290/1‑150 (MQ=255)
                                                                                                                                  cGAAAGTTATCCGCCCCCCGCCGCCGCCCCCAAAAAAACTTAAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATAAGATTTTAATAACAGGGGAATTATGATGTTAAATTAATGGCACCAGCCGCTGCCCCCATTTGTTaaa                      >  6:653119/1‑150 (MQ=255)
                                                                                                                                     aaGTTATCCGCCCCCGGCCTCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCGGCATTAGGCTATGGCAAGGTGATCAGATTTTCTTCACAGGGGAAGTATGATGTTAAATGCATGGCACCTGCCGGGGCCCCCATTTGTTAGAcaa                   >  1:301273/1‑150 (MQ=255)
                                                                                                                                        ttATCCGCCCCCGTCCTCCTCCCCCAAAAAACCTTCAGATGATGAGTGATCCTGCATTACGCTATGGCAAGGTGATCAGATTTTAATAACAGGGGAATTATGATGTTAAATTCATGGCACCTGCCGGTGCCCCCATTTGTTAaaaaaaca                >  5:899966/1‑148 (MQ=255)
                                                                                                                                        ttATCCGCCCCCGGCCTCCGCCCCCAAATATACTTCAGATGATGAGTGATGCTGCATTAGGCTATGGCAAGGTGATAAGATTTTCATCACAGGGGAATTATGATGTTTAATGCATGGCACCGGCCGGGGGCCCCCTTTGTTAAAaaaagc                >  4:97927/1‑150 (MQ=255)
                                                                                                                                          aTCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaa              <  7:1150157/150‑1 (MQ=255)
                                                                                                                                            ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGa           <  1:858532/151‑1 (MQ=255)
                                                                                                                                              gCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGGCCCCATTTGTTAAAcaaagcaa              >  4:572295/1‑146 (MQ=255)
                                                                                                                                              gCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATc         <  3:1637587/151‑1 (MQ=255)
                                                                                                                                              gCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATc         <  7:707050/151‑1 (MQ=255)
                                                                                                                                              gCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATc         <  7:618166/151‑1 (MQ=255)
                                                                                                                                               cccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATAAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATc         >  1:1148697/1‑150 (MQ=255)
                                                                                                                                               cccacGTGCTCCTCCCCCAAATTTCCTTCAGTTGATGAGTGATCCTGCATTAGGCTATGGGAAGGTGATCAGATTTTCATTACAGGTGAATTATGATGTTATATGCATGGCACGTGGCGGTGCCCCCATTTGTTAAACAAATCATTGATCa        <  4:1317792/151‑1 (MQ=255)
                                                                                                                                                ccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTTCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCaa       <  5:451152/151‑1 (MQ=255)
                                                                                                                                                ccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCaa       <  4:1013152/151‑1 (MQ=255)
                                                                                                                                                ccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCaa       <  5:1284182/151‑1 (MQ=255)
                                                                                                                                                 cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAAc      <  2:1562730/151‑1 (MQ=255)
                                                                                                                                                    gTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGc   <  5:790247/151‑1 (MQ=255)
                                                                                                                                                     tcctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCt  >  2:279183/1‑151 (MQ=255)
                                                                                                                                                     tcctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCt  >  1:697218/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
TTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCT  >  NC_000913/422281‑422579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑