Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913696,4700CT78.6% 571.2 / 126.4 292noncoding (35/75 nt)glnXtRNA‑Gln
Reads supporting (aligned to +/- strand):  ref base C (5/54);  new base T (83/134);  total (104/188)
Fisher's exact test for biased strand distribution p-value = 3.65e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 292 total aligned reads displayed.

CGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACC  >  NC_000913/696322‑696613
                                                                                                                                                    |                                                                                                                                               
cgcgGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAg                                                                                                                                               <  7:704376/151‑1 (MQ=255)
  cgGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGaa                                                                                                                                             <  2:1597616/151‑1 (MQ=255)
  cgGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGa                                                                                                                                              >  5:739296/1‑150 (MQ=255)
  cgGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGa                                                                                                                                              >  2:121718/1‑150 (MQ=255)
  cgGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATAAGa                                                                                                                                              >  2:591548/1‑150 (MQ=255)
     cAGCGTCTCTTCAGACTTTGAAGGATTTCACGTGTCAACATCGCATTAGACATTGTATCAACGCACAAAAGGATAACGCTCGCCGAAGCGAGCTTTTTTAATTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                                                                                          <  4:1335388/151‑1 (MQ=21)
     cAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATcc                                                                                                                                          <  7:1054148/151‑1 (MQ=255)
      aGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCg                                                                                                                                         <  3:342043/151‑1 (MQ=255)
      aGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGACGGAATAAGAATACg                                                                                                                                         >  1:906339/1‑151 (MQ=255)
       gCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCgg                                                                                                                                        <  3:44528/151‑1 (MQ=255)
         gTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTg                                                                                                                                      >  5:512904/1‑151 (MQ=255)
           cGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGGCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGc                                                                                                                                     <  5:172096/150‑1 (MQ=255)
           cGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGcc                                                                                                                                    <  5:1087776/151‑1 (MQ=255)
           cGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGc                                                                                                                                     <  1:1598641/150‑1 (MQ=255)
           cGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGc                                                                                                                                     <  1:565327/150‑1 (MQ=255)
            gCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCt                                                                                                                                   >  7:411800/1‑151 (MQ=255)
             cATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCtt                                                                                                                                  <  5:1342038/151‑1 (MQ=255)
             cATCAGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCtt                                                                                                                                  <  7:187441/151‑1 (MQ=255)
              aTCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTa                                                                                                                                 <  1:1049059/151‑1 (MQ=255)
               tcagACATTGAAGGATACCACGTGTCAACATCGCATTCGAGATTGCATGAACGCAGAAAAGCATAAAGCTCGCGGAATCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCtt                                                                                                                                  <  2:1110379/146‑1 (MQ=37)
                ccGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTAcc                                                                                                                               <  3:660490/151‑1 (MQ=255)
                 cGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAGAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCg                                                                                                                              >  6:1426536/1‑151 (MQ=255)
                   aCATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCt                                                                                                                            >  6:1624825/1‑151 (MQ=255)
                   aCATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCt                                                                                                                            <  4:1229899/151‑1 (MQ=255)
                    aaTTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCtt                                                                                                                           <  6:929775/150‑1 (MQ=255)
                     aTTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTg                                                                                                                          <  3:1606131/151‑1 (MQ=255)
                     aTTGAAGGAATAGACTTGTCAACCTCGCATTCGAGATTGAATTTACGCATAATAGGTAAAATCTCTCCGTACCGTGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTg                                                                                                                          <  2:1241641/151‑1 (MQ=18)
                      ttGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTgg                                                                                                                         >  1:1530595/1‑151 (MQ=255)
                       tGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTgg                                                                                                                         <  3:518959/150‑1 (MQ=255)
                       tGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTAAATGTGGCAGGGTTACGAGGATTCGAACCTCGCAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGc                                                                                                                        >  3:407441/1‑151 (MQ=255)
                        gAATGATAATACGAGTCAACCTCGCATTCGAGATTGAATTAACGCAGAAACGGATAAAGCTCCCCGAACCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                                                                                        <  8:1055146/150‑1 (MQ=21)
                        gAAGGATTTGAGGTGTAAACAGAGCATTAGACATTGAATGAACGCATAAAAGGATAAATTTCTCGGAAGGGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                                                                                                       <  4:414712/151‑1 (MQ=18)
                        gAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCg                                                                                                                       <  7:1196424/151‑1 (MQ=255)
                          atgatcataCGTGTAAACTGCGCCTTCGAGCTTGTATGTACCCAGAAATGCATAAAGCTCGACGAACCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGAt                                                                                                                     <  6:791903/143‑1 (MQ=18)
                           ggagcataCGTGACACCAGCGCATTCCAGATTGCCTCAACGCCGAAACGCACCACGCTCGCGGAACCGAGCTTTTTTAATTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATa                                                                                                                    <  3:1166320/144‑1 (MQ=17)
                           ggATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGAt                                                                                                                     >  6:1117716/1‑150 (MQ=255)
                           ggATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATa                                                                                                                    <  4:847441/151‑1 (MQ=255)
                             aTAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATAcc                                                                                                                  <  4:907192/151‑1 (MQ=255)
                             aTAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATAcc                                                                                                                  >  1:1244180/1‑151 (MQ=255)
                              tAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATAccc                                                                                                                 >  3:880866/1‑151 (MQ=255)
                               aaGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATAccc                                                                                                                 <  6:388387/150‑1 (MQ=255)
                               aaGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATAcccc                                                                                                                <  8:1475423/151‑1 (MQ=255)
                                aGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATAcccc                                                                                                                >  6:45861/1‑150 (MQ=255)
                                aGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATAcccc                                                                                                                >  8:296808/1‑150 (MQ=255)
                                aGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCa                                                                                                               <  3:1486079/151‑1 (MQ=255)
                                 caCGAGTCAACCTCGCATTCCAGATTGTCTGAACGCAGAAAAGCAAAACTCTCGAGGAACCGAGCTTTTTTAATTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCaa                                                                                                              <  8:1111367/150‑1 (MQ=18)
                                 aaCGTGTCAACTTCGCATTCGAGATTGTATGAACGCAGAAAAGCTAAAAGCTCTCCGAATCGAGCTTTTTTAATTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCaa                                                                                                              <  2:1297398/150‑1 (MQ=21)
                                  atttgtCAACTTCGCATTAGAGATTGTATTTACGCATAATAGCTAAAATTTCTACGTATGGTTCTTTTTTAATTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCaa                                                                                                              <  2:96976/147‑1 (MQ=9)
                                    tagtCAACTTTGCATTCGAGATTGTATTTACGCATAAATGGATCACTCTCTACGAATCGTTCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAAca                                                                                                            <  4:225956/148‑2 (MQ=14)
                                    gtgtCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTg                                                                                                           <  5:324762/151‑1 (MQ=255)
                                    gtgtCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTg                                                                                                           <  7:22195/151‑1 (MQ=255)
                                      gtCTACATCGCATTCGACATTGTATGAACGCACAAATGGATAAATCTCTCCGTATCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAAcaaa                                                                                                          <  2:43893/150‑4 (MQ=18)
                                      gtCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTggg                                                                                                         <  8:431720/151‑1 (MQ=255)
                                      gtCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTAGGAATGCCGGAATAAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAAcaaat                                                                                                         >  4:506107/1‑147 (MQ=255)
                                        cAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTg                                                                                                       <  4:1410097/151‑1 (MQ=255)
                                         aaCAGCGCATTCCACATTGAATCAACGCAGAAACGCAACACTCTCCACGAACCGCGCTTTTATAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAAcaaattgg                                                                                                      <  8:69520/151‑8 (MQ=17)
                                          ccttCGCATTCGACATTGTATCAACGCAGAAAAGCAATAAGCTCGCGTAAGCGATGTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCaataaccgggc                                                                                                     <  8:266947/148‑10 (MQ=17)
                                          aCATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAAcatattgct                                                                                                     >  8:861648/1‑143 (MQ=255)
                                          aCATCGCATTAGAGATTGCAGAAACCCCCAATGGCAGAAATCTCGCCGTACCGAGCTTTATTAATTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAAcaaatt                                                                                                        <  5:1149110/148‑6 (MQ=11)
                                               gCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAAcaaattgcttttg                                                                                                 >  3:250598/1‑138 (MQ=255)
                                                cATTCGACATTGAATCAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACa                                                                                               <  6:830514/151‑1 (MQ=255)
                                                 aTTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATAAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTCGTTGCTCTTGCa                                                                                               >  7:1614133/1‑150 (MQ=255)
                                                  ttCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTCGGTGCACTTACa                                                                                               >  1:93886/1‑149 (MQ=255)
                                                   tCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAg                                                                                             <  5:1572787/150‑1 (MQ=255)
                                                    cGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGt                                                                                           <  1:320052/151‑1 (MQ=255)
                                                     gACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTa                                                                                          <  8:838584/151‑1 (MQ=255)
                                                     gACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGTGTGCTCttcaaattt                                                                                           >  5:12243/1‑143 (MQ=21)
                                                     aaCATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTa                                                                                          <  1:1078957/150‑1 (MQ=255)
                                                      aCATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTaa                                                                                         <  3:1441469/151‑1 (MQ=255)
                                                          tgaatgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCg                                                                                      <  8:640423/150‑1 (MQ=255)
                                                           gaatgaaCGCAGAAAATCAAAAAGCTCGCCGAAGAGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGt                                                                                     <  2:777806/150‑1 (MQ=255)
                                                                  cGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATa                                                                             <  3:1369467/151‑1 (MQ=255)
                                                                    cAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATaaa                                                                           <  4:727331/151‑1 (MQ=255)
                                                                      gAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAtt                                                                         <  7:1554284/151‑1 (MQ=255)
                                                                       aaaaGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTg                                                                        <  3:581167/151‑1 (MQ=255)
                                                                            cAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCACCTGGGTGCACTTACAAGGTAAGCGTCTTGAATATATTGGCTgg                                                                   >  7:1393452/1‑151 (MQ=255)
                                                                             aaaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTggg                                                                  <  7:823885/151‑1 (MQ=255)
                                                                               aaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGTCTGGGGt                                                                <  8:537951/151‑1 (MQ=255)
                                                                                aaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTa                                                               <  8:1622705/151‑1 (MQ=255)
                                                                                 aGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGGGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTAc                                                              <  7:960349/151‑1 (MQ=255)
                                                                                     cGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGa                                                            <  2:274167/149‑1 (MQ=255)
                                                                                     cGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGa                                                            <  1:1405821/149‑1 (MQ=255)
                                                                                     cGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGa                                                            <  4:594663/149‑1 (MQ=255)
                                                                                       ccGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGAt                                                        <  5:987155/151‑1 (MQ=255)
                                                                                       ccGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGAt                                                        <  6:1409585/151‑1 (MQ=255)
                                                                                         gAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCCACTGGGTGCACTTACAAGGTAAGCTTCTTGAATAAATTGGCTGGGGTACGAGGATTc                                                      <  5:860906/151‑1 (MQ=255)
                                                                                           cgCGAGCTTTTTTTTTGTGGCGGGGGGAGGGGGTGTGGACACTCTGAATGAGGGTATTAGTATACGGTGCCTTAGAGATTGGCGCTACCCCCACTGGGTGCACTTACCAGGTACGGGGCTTTATTAATTTGGCTGGGGTACGAGGATTCGa                                                    <  8:1318281/150‑1 (MQ=255)
                                                                                           agctagctTTTTTAATGTGGCTGGGGTACGCGGTCTCGATACTCGGAATGCCTGAACTTGTATACCGGGCCTTAGAGCTTGGAGCTACCCCAACTTGGTTCACTTACAAGGTATGGTGCTTTTATAAATTGGCTGGGGTACGAGGATTCg                                                     <  2:147520/150‑1 (MQ=255)
                                                                                            gCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTTAATAAATTGGCTGGGGTACGAGGATTCGaa                                                   <  7:529947/151‑1 (MQ=255)
                                                                                             cGAGCTTTTTTAATGTTGATGGGGTACGAGGATTCGATACTCTGAATGCCTGAATTAGAATCCTGTGCCTTACCGCTTGTCGATACCCCAACTGGGTGCACTTACAAGGTAAGGGGCTTTAATAAATTGGCTGGGGTACGAGGATTCGAAc                                                  <  6:1188688/151‑1 (MQ=255)
                                                                                             cGACCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTCGGTGCACTTACAAGGTACGCCTCTTGAATAAATTGGCAGGGGTACGAGGATTCGAAc                                                  >  5:96857/1‑151 (MQ=25)
                                                                                                     tttAATGTGGCTGGGGTACGAGGATTCGAACCAAGCAATGAAGCAATTAGAATACGGTAACATACAGCATCGAGATACACCAAAAGCTTGAAGAAAAAAGATAAGAAAATAAAATAAAGTGGCTGGGGTACGTGGAGTCGacgggagggct                                          >  2:826710/1‑141 (MQ=255)
                                                                                                      ttAATGTGGCTGGGGTACGAGGATTCGATACTCGGAATGCCTGAATTAGAATCCGGTGCCTTACCGCTTGTCGATACCCCTACTGGGTTCACTTTCAAGGTAAGCGTCTTTAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAAt                                          <  4:1667266/150‑1 (MQ=25)
                                                                                                                 ggggGACGAGGATTCGAAACTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTTGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGa                              <  7:276651/151‑1 (MQ=255)
                                                                                                                        gAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGAGACTTACCGCTTGGCGATACCCCAACTGAATGCAATTAAAATGTAAGCATCTTTAATAAAAAAGCAGAGGTACGAGGATACGAACGGCGGAGTGCCTGATACGGCATCCGGt                       >  1:1153619/1‑151 (MQ=9)
                                                                                                                         aGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGTGTGGACTTTCATGTTAAGCAGCTTTAAGAAAGTGGCAGGTTTTCGTGGATTCGACGCGAGGAGTGCCTGAGTCAGAATCCTGtt                      >  7:488420/1‑150 (MQ=9)
                                                                                                                         aGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGGTGCAATTCCAAGTTAAGCGTCTAGAATAAATAGGCTGGGGTACGTGGCTTCGACCGTCGGACTGCCggggtccgcagcggtgt                      >  3:93956/1‑136 (MQ=18)
                                                                                                                            ggtagatccTCTGAATGACGGAAGTAGTATCCCGCGCCTTACAGCTTGCCGATACCCCAACTTGGTGAACTTTCTAGGTAAGCTTCTTTAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCt                   <  3:1213731/144‑1 (MQ=18)
                                                                                                                                 aaCCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGGTGCAGTTACAAGGTAAGCGGATTGAATAAATTTTCTGGGGTTCGAGGATTCGAACCTCGGAATGCCGGTATCACAATCCgtaggattaccg              >  3:186498/1‑140 (MQ=21)
                                                                                                                                          aTGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGAGTGGTCTTTCAAGGTAAGCGGCTTTAATATAGTGTCTGGGGTTCGAGGCTTCGAACGTCGGATTGCCTGAGTCAGCATCATGTGCATTCCTGGTTGGCGAt     >  7:1342626/1‑151 (MQ=16)
                                                                                                                                                    |                                                                                                                                               
CGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACC  >  NC_000913/696322‑696613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑