Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,818,1580TA29.0% 274.5 / 116.9 187intergenic (‑23/+40)argZ/argYtRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base T (64/68);  new base A (17/37);  total (81/106)
Fisher's exact test for biased strand distribution p-value = 3.58e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.12e-01

Only 100 of 201 total aligned reads displayed.

TAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTT  >  NC_000913/2818009‑2818305
                                                                                                                                                      |                                                                                                                                                      
tAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTActgct                                                                                                                                                        <  7:928216/150‑1 (MQ=255)
 aaTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTActgctg                                                                                                                                                       <  1:446891/150‑1 (MQ=255)
  aTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑t                                                                                                                                                   >  5:347486/1‑151 (MQ=255)
  aTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑t                                                                                                                                                   <  7:665383/151‑1 (MQ=255)
    tCCTCTAGCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑ta                                                                                                                                                  <  6:1308951/150‑1 (MQ=255)
    tCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑ta                                                                                                                                                  <  6:1273973/150‑1 (MQ=255)
     ccGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑tat                                                                                                                                                 <  3:653228/150‑1 (MQ=255)
     ccGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑tat                                                                                                                                                 <  8:1173024/150‑1 (MQ=255)
     ccGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCAGA‑‑TAtt                                                                                                                                                >  8:1297367/1‑151 (MQ=255)
      cGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑ttttt                                                                                                                                               >  3:194409/1‑150 (MQ=255)
      cGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTACTTTACTGCTGA‑‑TAttt                                                                                                                                               >  8:698982/1‑150 (MQ=255)
       gCTATCTCTTCGATACCTTCATTGCTGAAGTTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGa                                                                                                                                              <  4:115204/151‑1 (MQ=255)
       gCTATCTCTTCGATACCTTCATTGCTGAAGCTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGa                                                                                                                                              <  4:107438/151‑1 (MQ=255)
       gCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTAATTTACTGCTGA‑‑tttttt                                                                                                                                              >  8:547024/1‑149 (MQ=255)
        cTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCTGA‑‑ttttttt                                                                                                                                             >  8:443198/1‑148 (MQ=255)
          aCCTCTTCCAACCCTTCATAGATGAAGATGTCGTAAAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑ttttttgat                                                                                                                                           <  7:116290/151‑6 (MQ=11)
             tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTAGTTTACTGCAGA‑‑TATTtataaacc                                                                                                                                        >  1:186478/1‑147 (MQ=255)
              ctTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TAtt                                                                                                                                                >  5:1057297/1‑142 (MQ=18)
              ctTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TAtt                                                                                                                                                <  1:1059870/142‑1 (MQ=18)
              ctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCg                                                                                                                                         <  7:1310804/149‑1 (MQ=255)
              ctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTAATTTACTGCAGA‑‑tttt                                                                                                                                                >  4:1639797/1‑142 (MQ=255)
              attCGATACCTTCATACCTCAATATTTCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑ttttttgataccg                                                                                                                                       <  3:1604517/150‑10 (MQ=11)
               ttCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTAGTTTACTGCTGA‑‑ttttagatccgaat                                                                                                                                      >  3:328171/1‑141 (MQ=14)
                tCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATAACGc                                                                                                                                        <  8:1620153/148‑1 (MQ=255)
                 gtttACCTTAATTTATGAATATTTAGTATTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGcta                                                                                                                                      <  6:305607/148‑1 (MQ=14)
                 ggtaaCCTTCATTTCTGAATATTTAGGATTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGcta                                                                                                                                      <  6:721243/147‑1 (MQ=11)
                    tACCTTCATTGTTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑t                                                                                                                                                   <  5:1442389/133‑1 (MQ=16)
                    tACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTTATAAAGCtaataa                                                                                                                                  >  5:1023940/1‑150 (MQ=255)
                    tACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTACTTTACTGCAGA‑‑tttttttatacctataga                                                                                                                                  >  2:417741/1‑136 (MQ=11)
                      ccTTCATTGCGGAAGATGTCGGAGTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGCTACTaaa                                                                                                                                 <  8:1330129/151‑1 (MQ=11)
                      ccTTAATTGCTGAAGATTTCGGATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGCTACTaaa                                                                                                                                 <  4:110229/151‑1 (MQ=11)
                        ttCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGcc                                                                                                                              >  1:924906/1‑150 (MQ=255)
                        ttCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTAATTTACTGCAGA‑‑TTTTtatatacctaaaaaaa                                                                                                                                >  2:1510534/1‑136 (MQ=11)
                         tCATTTCTGAATATTTCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGCTACTaa                                                                                                                                  <  6:4472/147‑1 (MQ=11)
                         tCATTTATGAATATTTAGTATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGc                                                                                                                               <  2:196216/150‑1 (MQ=14)
                          cATTCCTGAAGATTTCGGATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCTGG‑AAACTTACTTTACTGCAGA‑‑ttttttgataccg                                                                                                                                       <  6:1054705/139‑10 (MQ=11)
                          aaTTTCTGAATATTTAGGATTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGccg                                                                                                                             <  4:1489370/150‑2 (MQ=14)
                          aaTTGATGAATATTTAGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGcc                                                                                                                              <  5:1541491/149‑1 (MQ=11)
                            atattGAAGATTTCGGATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTAAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCgta                                                                                                                           <  1:151101/147‑1 (MQ=11)
                              gCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCTCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCa                                                                                                                        <  7:701721/150‑1 (MQ=255)
                                 gcatttcaactattatatGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCgtat                                                                                                                          <  5:1097817/133‑1 (MQ=17)
                                 gAATATATCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCa                                                                                                                        <  7:1203615/149‑1 (MQ=14)
                                 gAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAg                                                                                                                      <  3:1141730/149‑1 (MQ=255)
                                  aataTTTAGGATAATATTGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGt                                                                                                                     <  4:701330/148‑1 (MQ=14)
                                  aaGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTaa                                                                                                                   <  5:902468/151‑1 (MQ=255)
                                   aGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAag                                                                                                                  <  5:922906/151‑1 (MQ=255)
                                    gATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAaga                                                                                                                 <  1:251014/151‑1 (MQ=255)
                                         cGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAg                                                                                                                                <  7:1567424/131‑1 (MQ=38)
                                         cGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATg                                                                                                             <  8:415345/150‑1 (MQ=40)
                                          ggAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGt                                                                                                           <  2:349593/151‑1 (MQ=39)
                                          ggAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTAGTTTACTGCTGA‑‑TATTTATAGAGCTACTAAACCGAGGTCAAGAAATAGAtatt                                                                                                           >  5:1248351/1‑148 (MQ=14)
                                           gagaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTATGAGATGGTg                                                                                                          <  2:836570/151‑1 (MQ=255)
                                           gagaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTg                                                                                                          <  1:348486/151‑1 (MQ=39)
                                            agaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGc                                                                                                         <  3:707498/151‑1 (MQ=39)
                                              aaTATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCAt                                                                                                       <  3:481556/151‑1 (MQ=39)
                                                tatGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTAGTTTACTGCTGA‑‑TATTTATAGACCTACTATAGCCATCTAAACAAAAAGATCGTgagaa                                                                                                      >  7:97014/1‑146 (MQ=11)
                                                        aTCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTAGTTTACTGCAGA‑‑TATTTATAGAGCTAGAATAGCCATATCAAGAATGAGATGGTgataaacggagggt                                                                                             >  5:774989/1‑138 (MQ=11)
                                                          ccGGTAGGATTCGAACCTCCGACCGCTCGTTTCGTAGCCGATTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGAtt                                                                                            <  6:1186280/150‑1 (MQ=255)
                                                          ccGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTActtctg                                                                                                                                                       >  4:1573224/1‑93 (MQ=38)
                                                          ccGGGAGGATTCCAACCTCCGACCGATCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATAGAGCGAATATAAACAAATAAAAAAAGAGATGGTGAAGAGGGGAGGAta                                                                                            >  1:149729/1‑149 (MQ=11)
                                                           cGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTAATTTACTGCAGA‑‑TATTTATAACGCTAAAAAAGCGAAATCAAGTAAGAGATGGTGAAACCGCGAGCAtaa                                                                                           >  1:519179/1‑148 (MQ=11)
                                                                ggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCAGA‑‑TATTTATAGCGCTCGTAAAGACATATCAAAAATGAGATGGTGCCTCACCGAGGATTAGacgcg                                                                                     >  5:1583482/1‑147 (MQ=11)
                                                                    tgtatacTCCGACAGCTCGTTTCGTTGCCGTGTTCTCTAACACCCTGTTTTACTTATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGAAACCGCTAATATAGCCATATCAAGTACCATATGGTGCATCCGGGAGGATTCGAACCTCCg                                                                                  <  1:955262/144‑1 (MQ=255)
                                                                     cGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTActgct                                                                                                                                                        <  2:1281109/81‑1 (MQ=38)
                                                                     cGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTActgct                                                                                                                                                        >  3:1273859/1‑81 (MQ=38)
                                                                     cGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACAGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGACGCGCCGA‑c                                                                               >  5:171782/1‑151 (MQ=255)
                                                                            ccGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTAATTTACTGCTGA‑‑TATTTATAACGCTAATAAAGCCATATAAACTAAGAGATGTTGAAACACGGAGGATTAGACTCGCCGAGCCGCTCg                                                                         >  2:482813/1‑151 (MQ=11)
                                                                              gACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGC‑AAATTTATTTTACTGCTGA‑‑TATTGATAACGCTACTAAAGCCATATCAAGTAACAGATGGTGAATACCCGAGGATTAGAAGCGCCGagccgccccat                                                                       >  5:217507/1‑141 (MQ=14)
                                                                                 cgctcggggccgaGCCGAGGACTCTATCCCGATGCGGTACGGATGCAGCGGG‑AAATTTATTTTACTGCTCA‑‑TATTCCAACCGCTAATACAGCAATATCACGTACCATATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGt                                                                   <  7:337125/139‑1 (MQ=16)
                                                                                 cGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑ta                                                                                                                                                  <  3:412656/73‑1 (MQ=255)
                                                                                     ggtggcggaGCCGCGTTGTCTATACCGCTGTTCAACTGAGGCAGCGGG‑TCATTTATTTTCCTGGAGA‑‑TATTCATACCCCTCATAGATCCATATCACGTATCATATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGcc                                                               <  5:492315/143‑1 (MQ=9)
                                                                                           atAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATAAAGTAAGAGATGGTGCATCCGGGAGGATTAGAACCTCCGA‑CCGCTCGGGTCGAACCCgctaa                                                          >  5:1028657/2‑146 (MQ=17)
                                                                                             aGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTTATACCGCTACTAAAGCCATATCAAATAAGAGATGTTGCATACGGGAGAATTAGACACTCCGA‑GCGGCGCGATCGTAGCCGATTaga                                                        >  1:694919/1‑148 (MQ=14)
                                                                                               ccGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TAtt                                                                                                                                                >  1:1084614/1‑61 (MQ=255)
                                                                                                cGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTCA‑‑TATTGATACCGCTACTAAAGCCATATCACGTACGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTAt                                                    <  7:1670151/151‑1 (MQ=255)
                                                                                                 gAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTtata                                                                                                                                            >  3:992511/1‑63 (MQ=255)
                                                                                                 gAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATa                                                                                                                                            <  2:996837/63‑1 (MQ=255)
                                                                                                        ctATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑tat                                                                                                                                                 <  8:1521875/51‑1 (MQ=255)
                                                                                                        ctATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑tat                                                                                                                                                 >  6:1523876/1‑51 (MQ=255)
                                                                                                        caaTCCCGATGCGCTACCGATCCATCGCG‑ACATTTAATTTACTGCAGA‑‑TATTCCAAACCCTACTTCAGCCATATCAAGTAACATATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGa                                            <  1:309083/149‑1 (MQ=17)
                                                                                                               gCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGAACCTCCGA‑CCGCTCGGTGCGTAGCCGCGGAGTATAGCCAGCTGAGCTACgg                                     >  3:957289/1‑151 (MQ=18)
                                                                                                                      tACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACgagatccg                               >  2:615352/1‑143 (MQ=255)
                                                                                                                       acatcagaagaggg‑AAATTTAATTTCCTGCTCC‑‑TCTTGGTACCGCTCATTTCTGAATATCTCGTATGATATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATc                              <  8:1077158/139‑1 (MQ=2)
                                                                                                                        cGGATGCATCGGG‑AAATTTAATTTACTGCTGA‑‑TATTGATAGACCTACTAAAGCCATATCAAGTAAGATAGCGTGCATCCGGGAGGATTCGAACCGCCGC‑CCGGTCGGTTAGAAGCCGAGTACGCTATCCAGCTGAGCTCCGGAGGccgcgg                            >  3:1463160/1‑146 (MQ=21)
                                                                                                                          taggcagtggg‑AAATTTATTTTACTGGTGA‑‑TATTGATTCCGCTACTAACGGAATATTAAGTATCATATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATc                              <  5:65224/139‑1 (MQ=17)
                                                                                                                            tctatggggaaaaGTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGa                          <  8:377091/140‑1 (MQ=21)
                                                                                                                               aTCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTCCACCCTAAGA‑CCCAACCCTTACTAGCCGATTAAAATATCAAGATGAGCTACGTAttgagagcgaaga                       >  6:376472/1‑138 (MQ=9)
                                                                                                                                  ggcctacgttatttTACTGCTGC‑‑TCTTGATTCCCGTCCTTTCGGCATATGATGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAacttg                    <  3:856813/142‑2 (MQ=14)
                                                                                                                                  agccttcgtgtatttCCTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAacttac                   <  5:418878/139‑1 (MQ=255)
                                                                                                                                    ggacagttaattTACTGCAGA‑‑TATTCCATCCGCTCCTATCGCCATATCAAGGATCAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAacttactt                 <  1:77283/145‑1 (MQ=14)
                                                                                                                                    gaaaaCTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCt                                                                                     <  8:336469/82‑1 (MQ=255)
                                                                                                                                      ccgatttttctACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACttacttta               <  7:476757/141‑1 (MQ=255)
                                                                                                                                             ttctACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTCCACCCTCAGA‑CCCAACCGATACTAGCCGATTAATCAATCCAGATGAGCTACGGATTCATAGGGAAaattacttaaagatcgt        >  4:479886/4‑142 (MQ=9)
                                                                                                                                               ctACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTc                                                                          <  8:1586523/82‑1 (MQ=255)
                                                                                                                                                tACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCg                                                              <  3:1305060/94‑1 (MQ=255)
                                                                                                                                                tACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGAGATGCCAAGAGCATACTCTACTCAAA‑CCGGTGGGGTCGTAGACTAGTACTCTATCGAGCTGAGATAATTATAAATAGGGAAACTTACTTTACTGGAGAgtt     >  6:656656/1‑151 (MQ=9)
                                                                                                                                                   tgctgA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGggagga                                                                                              <  3:1485570/60‑1 (MQ=35)
                                                                                                                                                    gctgA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCgg                                                                        >  6:502962/1‑80 (MQ=255)
                                                                                                                                                    gctgA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCgg                                                                        <  8:502346/80‑1 (MQ=255)
                                                                                                                                                    gctgA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGAtttttt  <  7:271554/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
TAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGA‑CCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTT  >  NC_000913/2818009‑2818305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑