Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,269,9940GT31.9% 188.4 / 25.4 161intergenic (+392/+631)yhaC/garKpentapetide repeats‑related protein/glycerate kinase I
Reads supporting (aligned to +/- strand):  ref base G (13/81);  new base T (3/41);  total (19/142)
Fisher's exact test for biased strand distribution p-value = 2.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 164 total aligned reads displayed.

TGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATA  >  NC_000913/3269844‑3270141
                                                                                                                                                      |                                                                                                                                                   
ggAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGGGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTg                                                                                                                                                     <  7:213059/150‑1 (MQ=255)
  aaaCCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGaa                                                                                                                                                   <  8:1473770/151‑1 (MQ=255)
  aaaCCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGaa                                                                                                                                                   <  5:1571660/151‑1 (MQ=255)
     cccGCCTTATCGGTTTATTACGTAAATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGGCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACt                                                                                                                                                <  2:569234/151‑1 (MQ=11)
     cccGCCGTACCGGTTTATTACGTACATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACt                                                                                                                                                <  6:1419389/151‑1 (MQ=255)
       cgccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGa                                                                                                                                              <  7:417974/151‑1 (MQ=255)
       cgccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGa                                                                                                                                              <  6:463007/151‑1 (MQ=255)
         ccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAcc                                                                                                                                            <  8:375972/151‑1 (MQ=255)
          cgGAACGGTTTATTACGTAGATCTGGTAAAACTGACCGATAAGCCTCTTTCTTTTGTGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCg                                                                                                                                           <  4:1214386/151‑1 (MQ=255)
           ggAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGa                                                                                                                                          <  8:751564/151‑1 (MQ=255)
            gAACGGTTTATTACGTACATCAGTTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGAt                                                                                                                                         <  3:1394583/151‑1 (MQ=255)
            gAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGAt                                                                                                                                         <  4:188549/151‑1 (MQ=255)
             aaCGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATa                                                                                                                                        <  6:1165035/151‑1 (MQ=255)
               cGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAg                                                                                                                                      <  7:1045920/151‑1 (MQ=255)
               cGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAg                                                                                                                                      <  2:562237/151‑1 (MQ=255)
                 gTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGcc                                                                                                                                    <  1:115522/151‑1 (MQ=255)
                   ttattaCGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCg                                                                                                                                   <  5:177821/150‑1 (MQ=255)
                        tcgttgaTCAGGTTTAACTGAGCGATAATACGCTTTCTTTTGGGTTTTTTGTCGTGGACAGTGATTCTTCTTTCTGCCCCTCCAAAAGTTAAAAGCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGctttc                                                                                                                              <  4:88044/144‑1 (MQ=11)
                         cgttcatcttttattaCTGACCGATAATCTGGTTTCTTTTGTGTTTTGTGTCGTGGACAGTCTTTCTTATTTCTGGCCCTCCAAAATTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGctttctt                                                                                                                            <  1:252891/136‑1 (MQ=11)
                           tACATAAGGTTTAACTGTGCGTTCAGGCGGTGTCTTTTGGGTATTGTGTCGTGGACAGTCATTCTTCTTTATGCCCCTCCAAAAGTATAAACCCGCCGTATCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGctttctt                                                                                                                            <  6:1290028/149‑1 (MQ=11)
                            aCATCAGGGAACACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGGGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg                                                                                                                         <  8:1312537/151‑1 (MQ=255)
                             cATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTTGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg                                                                                                                        <  4:755477/151‑1 (MQ=38)
                               acAGTTAAAACTGTCCGATAAGCCGCTTTCTTTTGGGTATTGTGTCGTGGACAGTGATTCATCTTTCTGCCCCTCCAAAAGTATAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGt                                                                                                                      <  1:514733/150‑1 (MQ=255)
                                tatgTAAAACTGACCGTTATGTTGGTTTCTTTAGGGTTAAGTCTCGTGTAAACTCTTACTAATTTATGGCACTCAAAAAGTACAAACCCGCCGAAGCGTTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGt                                                                                                                      <  8:1205314/147‑1 (MQ=2)
                                attgttaaactttgcGATAAGCTGTTTTCTTTTTGGTATAGTGTCGTGGAATGTCTTTCATCTTTCTGGCACTCCAAAAGTAAAAACCCGCCGAATCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGta                                                                                                                     <  6:93225/137‑1 (MQ=11)
                                  tgTAAGACTTAGCGATAAGTTGCTTTCTTTTTTTTTTTGTCTCGTGGAAAGTGTTTCCTCTATCTGCCCCTCCAAAAGTTTAAACCCGCCGAATCGGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGtat                                                                                                                    <  1:418551/149‑1 (MQ=2)
                                     aacaCTGACCGATAAGCCGCTGTCTTTTTGTTATAGTGTCGGCGACAGTCTCTCATCTTTCTGCCACTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAgtg                                                                                                                <  5:561491/148‑1 (MQ=11)
                                     aaaaCTGACCGATAAGCCGCTTTCTTTTGGCTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAATTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAgtg                                                                                                                <  1:1137600/151‑1 (MQ=25)
                                        actttgcGATAATCCGCTTTCATTTTTTTTTAGTGTCGTGGAAAGTGATTCTTCTATCTGCCACTCCAAAAGTAAAAACCCGCCGAATCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCg                                                                                                             <  1:393795/145‑1 (MQ=11)
                                        aCTTACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCg                                                                                                             <  7:498642/151‑1 (MQ=25)
                                        aCTTACCGATAAGCCGCTTTCATTTGCCGAGAGTGTCGGCGCCAGTCATTCATATTTATGCCCCTCAAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCg                                                                                                             <  3:610950/151‑1 (MQ=11)
                                           gACCGATAAGCCGCTTTCTTTTTCGTATAGTGTCGGGGACAGTCATTCATCTATCTCCCACTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTg                                                                                                           <  5:1338931/150‑1 (MQ=11)
                                           gACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGAAATTAATCTATAAGCCAAGCCAAAAAAAAAAACACAAAGAATAGGGATATTACGTAAAACAAGTGATAAAAAACAATAAGCCGCGTTATTTgggggactgggtggggg                                                                                                          >  6:171507/1‑138 (MQ=2)
                                             cccataatgctcttgctttTGTCTATAGTGTCGGCGACAGGCACACATATTTCTGCCCCTCCAAAAGTAAAAACCCGCCGCAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAc                                                                                                        <  5:175234/140‑1 (MQ=11)
                                                 tAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTAATCTTTCTCCCCAAACAAAAAAAAAAACAAGCCGAAACCCCTTTTTACGTAAAACAAGTGAAAAATAACCAAAAGCAGCTTTCATTAGAGTAGAgtgatgaataaaagg                                                                                                    >  1:1338327/1‑139 (MQ=2)
                                                  aaggagCTTTCTTTTTCATAGAGTGTCGCGGAATGCGATTCATATATCTGCCACTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGtca                                                                                                   <  8:691213/146‑1 (MQ=11)
                                                     gtgCTTTCTTTTTTGTATAGTCTCGACGAAACTCATTCATATATCTGCCACTCCACAATAAAAAACCCGCCGCAGCGGTTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGtcattc                                                                                                <  5:67908/149‑1 (MQ=11)
                                                     ccGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGTAAAAAACCCCCGAAGCGGGGTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGGAAAGTTTctggggagcggacac                                                                                                >  3:462131/1‑137 (MQ=11)
                                                       ggattctttTGCGTAACGTGTCGCCGACAGCGTTTCTTCTTTCTGCCACTCAAAAAGTAAAAACCCGCCGCAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGtcattcat                                                                                              <  5:237819/148‑1 (MQ=11)
                                                       gCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTGTGCCCCAACAAAACAAAAAACACGCCGAAGCGGGTTTTTACGTAAAACAGGGGCAACTGACCCATAACCCCCCTTCTTTAGGGGACAGAGTGGTGGGTAGtcgtgaa                                                                                               >  3:159781/1‑145 (MQ=2)
                                                           tctttttttgctaGTGTCGTGGAAAGTCTTTCTTCTTTCTGGCACTCAAAAAGTAAAAACCCGCCGAATCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCtt                                                                                           <  1:844494/139‑1 (MQ=11)
                                                           tctttTGGGGAAAGTGTCGGGGACAGCGAGTCAACTTTCTGCCCCTCCAAAATTAAAAACCCGCCGAATCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCtt                                                                                           <  5:1348166/150‑1 (MQ=11)
                                                                 ggctataGTGTCGGGGACAGTCAGTGCTATTTCTCGCACTCAAAAAGTATAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGcc                                                                                     <  1:1220218/147‑1 (MQ=11)
                                                                    cccccccgcGTCGACAGTCATTCATCTTTCTGCCCCTCCAAAATTAATAATAATCATAAGGGGTTTTTTAAGTTAAACAGTTTTATCTTAACTATTAGCCTCTTTGTTTTTGTTATTTTGTCGTGGACAGTCATTCATCTTTCTGCCCCTc                                                                                 <  2:448757/143‑1 (MQ=2)
                                                                     attgtgtCGTGGAAAGTGTTTTTTATATCTGGCACTCCAAAAGTACAAACCCGCCGCATCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTc                                                                                 <  1:201967/147‑1 (MQ=11)
                                                                        gtgtCGGCGACAGGCACTGATCTTTCTGCCCCTCCAAAAGCACAAACCCGCCGAACCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaa                                                                             <  3:465986/151‑1 (MQ=11)
                                                                         tctCGTGGACTGTGACTCTTATATCTCGCACTCCAAAAGTTCAAACCCGCCGTATCGTGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaa                                                                             <  3:257455/148‑1 (MQ=11)
                                                                            cGTCGAAAGTGATTCCTATTTCTGCCACTCCAAAAGTAAAAACCCGCCGTACCGGGTTTTTACGTAAAACAGGTTAAACTGAGCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGc                                                                          <  8:659222/150‑1 (MQ=11)
                                                                                 ccAGTTATTCAACTTTCTGCCCCTCCAAAAGGAAAAACCCGCCGTAGCGGGTTATTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAc                                                                    <  5:500475/150‑1 (MQ=255)
                                                                                    ccggcagtaatttCTGGCACTCAAAAAGTAGAAACCCGCCGGACCGGGTTATTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccg                                                                 <  7:875442/141‑1 (MQ=11)
                                                                                        tagaacTTTATCCCACTCAACAAGGAAAAACCCGCCGCAGCGGTTTTTTACGTAAATCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgccg                                                              <  7:1432180/145‑1 (MQ=11)
                                                                                         ttatCTTAATGCCCCTCAACAAGATTAAACCCGCCGTTTCTTTTTTTTACGTAAATCATGTTAAACTGACCGATAAGCCGCGTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGa                                                             <  6:536128/148‑1 (MQ=11)
                                                                                          gatctatctcgcacTCAAAAAGGAAAAACCCGCCGCAGCGGTTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGaa                                                            <  5:687245/137‑1 (MQ=11)
                                                                                          aatCTTTCTGCCCCTCCACAATTAAAAACCCGCCGAATCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAg                                                           <  7:448475/150‑1 (MQ=255)
                                                                                             cTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTAACTAAAAAACGTCAAAATGCCAAATAAGCAGCATAATTTTGTGTATATAGTAGTGGGCCGTAAATAAAAAAAAAACCCCGCCAAAAGCAAAAACCCGCCGGAGCgg                                                        >  1:265861/1‑151 (MQ=2)
                                                                                             atatCTGGCACTCAACAATTAAAAACCCGCCGTATCGGTTTTTTACGTACAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCg                                                         <  2:335453/147‑1 (MQ=11)
                                                                                             ataaaggCCACTCAAAAATTATAAACCCTCCGAATCGGTTTATTACTTAAAACATTTTATACTGACCGATAAGCCGCTTTCTTTTGTGTATAGTGTCGTGGAAAGTCATTCATCTTTCTGCCACTCCAAAATCAAAAACCCGCCGAAGCgg                                                        <  4:81475/145‑1 (MQ=11)
                                                                                               ttCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGcc                                                                                                                                    >  2:25708/1‑73 (MQ=38)
                                                                                                aagcccccTCAACAATCAAAAACCCGCCGCAGCGGGTTATTACGTACAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtt                                                     <  3:192876/147‑1 (MQ=11)
                                                                                                 cgcgcccTCCACAAGCACAAACCCGCCGCACCGGGTTTTTACGTACAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                                    <  3:1139651/147‑1 (MQ=11)
                                                                                                  tGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTAGAGTGTCGTGGCGAGTCCTTCATGGTTGGGCGCCGGCAAAAGCAAAAACCCGGCGCAGggggggtt                                                   >  5:1188861/1‑147 (MQ=11)
                                                                                                  ggCCACTCAAAAAGCATAAACCCGCCGTATCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtttt                                                   <  6:889251/150‑1 (MQ=11)
                                                                                                         ccAAAAGTAAAAACCCGCCGAAGCGGGTATATACGTAAACCATCTTAAACTGAAAGATAAGCCGATTTCTTTTAGGTATAGTTTCTTGGCCAATCATTAAAAAATAAGACCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttggtaggg                                              >  1:720754/1‑142 (MQ=2)
                                                                                                         ccAAAAGGAGAACCCCGCCGCACCGGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTa                                             <  8:43586/150‑1 (MQ=11)
                                                                                                         ccAAAAGCACAAACCCGCCGCACCGGGTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGt                                              <  3:709962/149‑1 (MQ=255)
                                                                                                         caacaattAAAAACCCGGCGTACCGGTTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTa                                             <  4:941991/143‑1 (MQ=11)
                                                                                                         caacaatcacaaaCCCGCCGCACCGGGTTATTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTa                                             <  3:1280810/140‑1 (MQ=11)
                                                                                                         caacaagtttaaaCGCGCCGGATCGGTTTTTTACGTAAAAGAGGTTAATCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGGAAAAACCCGCCGAATCGGGTTTTTACGt                                              <  4:1267593/139‑1 (MQ=11)
                                                                                                         caaaaattttaaaCCCGCCGCATCGGTTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTaa                                            <  6:282390/141‑1 (MQ=11)
                                                                                                         caaaaataaaaaaCCCGCCGTATCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTaa                                            <  2:672680/143‑1 (MQ=11)
                                                                                                          cacaatcacaaaCCCGCCGCACCGGGTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTaaa                                           <  7:1150927/142‑1 (MQ=11)
                                                                                                          cAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAGATTCATTAAGATTTATTAACAAACAAAAGCAAAAACACGACGAAGCGGGTTTTTACGTaa                                            >  4:550189/1‑150 (MQ=11)
                                                                                                           aaaaGCACAAACCCGCCGCACCGGGTTATTACGTACAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAc                                          <  3:394681/151‑1 (MQ=11)
                                                                                                            aaaGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccg                                                                 <  6:232962/127‑1 (MQ=17)
                                                                                                            aaaGCACAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAcc                                         <  7:664627/151‑1 (MQ=18)
                                                                                                              acgaaaaaCCCGCCGGACCGGTTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAg                                       <  8:1276571/148‑1 (MQ=255)
                                                                                                                 ttaaaCCCGCCGTATCGGTTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGt                                     <  4:1125797/148‑1 (MQ=11)
                                                                                                                 acaaaCCCGCCGCACCGGGTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTg                                    <  3:736337/149‑1 (MQ=255)
                                                                                                                 acaaaCCCGCCGAACCGGGTTATTACGTACAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTg                                    <  7:83832/149‑1 (MQ=255)
                                                                                                                  taaaCCCGCCGTATAGGTTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAg                                       <  1:1340312/146‑1 (MQ=11)
                                                                                                                  aaaaCCCGCCGTATCGGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGa                                   <  6:1158384/151‑1 (MQ=255)
                                                                                                                  aaaaCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGCAGCTGAGCGATCGGCGGCTGTCGTTTGGGTATAGTGTCGTGGGGAGTCCGTCTTCTTTGTGCAACACCAAAAGGAAAAACCCGCCGGGGGGGGgttttggggtgcgggggggg                                   >  8:1242966/1‑136 (MQ=2)
                                                                                                                   aaaCCCGCCGTATCGGTTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGaa                                  <  2:736426/151‑1 (MQ=255)
                                                                                                                    aaCCCGCCGCATCGGTTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGaaa                                 <  6:1392651/151‑1 (MQ=255)
                                                                                                                    aaCCCGCCGAATCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTTTAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGaaa                                 <  1:1054618/151‑1 (MQ=255)
                                                                                                                      cccGCCGTATCGGTTTATTACGTAAAAGAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACt                               <  1:847233/151‑1 (MQ=11)
                                                                                                                       ccgccgGAACGGGTTTTTACGTACAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGTCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTg                              <  3:435998/151‑1 (MQ=255)
                                                                                                                        cgccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGACAGtcattcat                                                                                              >  2:1428353/1‑86 (MQ=18)
                                                                                                                           cgtatcGGTTTTTTACTTAAATGATGTTTAACTGACCGATAAGCAGCTTTCTTTTGTGTTTATTGTAGTGGAGATTCATTCATCTTTCTGACCCTAAAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCg                          <  1:1385287/146‑1 (MQ=11)
                                                                                                                            gtatcgttttttaACTTACAACATTTTAAACTGACCGATAAGAAGATTTCTTTTGTGTATAGTGTCGTGGACAGTCATTCATATTTCTGCCACTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGa                         <  4:1664655/143‑1 (MQ=11)
                                                                                                                              atcGGTTTATTACGTAGAACATGTTTAACTGAGCGATAAGCCGCTTTCTTTTTTGTATAGTGTCGTGGACAGTGATTCATCTTTCTGGCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATa                       <  4:1391461/149‑1 (MQ=11)
                                                                                                                              agagggTTTTTACGTAAAACCGGTTAAACTGACCGATAAGCAGCTTTCTTTTGGGTATAGTGTCGTGGACAGGCATTCATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATa                       <  5:1173353/148‑1 (MQ=11)
                                                                                                                              aGCGGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCATTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCACTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATa                       <  5:751196/151‑1 (MQ=255)
                                                                                                                                 ggttttttACGTAAAACACGTTAAACTGACCGATCAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACTGTGATTCTTCTTTCTGCCCCTCCAAAATCATAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGc                    <  3:684660/148‑1 (MQ=11)
                                                                                                                                 gggTTTTTACCTAAAACAGGTGAAACTGATCTATATGCCTCTTTCTTTTGGGTATAGTGTCGTTTACAGTAAATCATCTTTCTTCCCCTCCAAAATCATATATCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGc                    <  4:798084/151‑1 (MQ=11)
                                                                                                                                  tttttttACGTACATCAGGTCTAACTGACCGATAAGCCGCTTTCTTTTGGGTATATTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAATCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATaa                      <  1:650058/146‑1 (MQ=11)
                                                                                                                                    ttattACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTGATATTTGTGGCCCGCCAAAAGGAAAAAACCCCCGAAGCGGGTTTTTACAGAAACGAAGAGAAAACGTCCGAAAACCCgt                 >  5:1264817/4‑150 (MQ=14)
                                                                                                                                      tttACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGTGGATAGAGTCGTGGCCAGGGAGTAATCTTTCTTCGCCTGCAAAAGAAAAAACCCGCCGCAGCGGGGTTTTACGTTAAGCCGGTGCAACGGTCGGCTGAGCCGcgg               >  7:415456/1‑149 (MQ=2)
                                                                                                                                               atcAGGTAAAACTGACCGATAAGCCGCTTTGTTTTGGGTATAGTGTCGTGGCCAGTCAGTAGATTGTGTGCCCCTCCAAAATTAAAAAACCGCCGAAGCGGGTTGTTTCGGTAACCCGGGGGAACGGGCCGGTCAGCCgggtgggtggggg      >  7:1441370/3‑139 (MQ=2)
                                                                                                                                                   ggTGAAAATGACATATACGCCGATTTTTTTTGTCTATTTTGCTGTTGAGAGTTATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtata  <  1:103172/151‑1 (MQ=11)
                                                                                                                                                      |                                                                                                                                                   
TGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATA  >  NC_000913/3269844‑3270141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑