Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,270,1670CG27.2% 327.5 / 53.4 243intergenic (+565/+458)yhaC/garKpentapetide repeats‑related protein/glycerate kinase I
Reads supporting (aligned to +/- strand):  ref base C (84/63);  new base G (54/1);  total (177/66)
Fisher's exact test for biased strand distribution p-value = 5.10e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 245 total aligned reads displayed.

TGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGC  >  NC_000913/3270035‑3270317
                                                                                                                                    |                                                                                                                                                        
tGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCGAGTCATTCATTCTTTTGCCCCGACAAAACCaa                                                                                                                                         >  5:416951/1‑150 (MQ=11)
       tcattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTCGCAAGTCAATCATATTTCCGCCCCGCCAAAATCAAAAACccgc                                                                                                                                 >  8:1170165/1‑151 (MQ=11)
         attcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGTGTATAGCGTCGTGGCTAGGCAATCAATTTTTACCACCCTCAAAAACAAAAACccgccg                                                                                                                               >  5:629519/1‑151 (MQ=11)
           tcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCCTCGTGTAGAGTCAATCATAATTGTGCCGCAACAAAAGCAAAAAccaggaga                                                                                                                              >  1:547898/1‑144 (MQ=11)
              tCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGGTTTCTGTAGGGTAGAGAGTGGGGGCAAGGCATTCAATATTGAGCGCCCTCaaaaacaaaaacacccccaacgc                                                                                                                          >  8:146700/1‑140 (MQ=2)
              tCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGAGTAGTGGCAAGGCAATCAAGATTTGGCGCCCCCAAAAACAAAAACccgcctcaggc                                                                                                                          >  8:370615/1‑145 (MQ=11)
                 ttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGCGTCGTGGCGAGGCAATCATGCTGGGGCGGCCGCAAAAGCAAAAACccgcggcaggcggg                                                                                                                       >  5:1598055/1‑141 (MQ=11)
                   cNGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTGGGGTAGAGCGTGTGGGCCAGGCAAGCATCCGGCTGCCCCCGCAAAACCAAAAACCCGCCG‑CAG‑Cgcgggg                                                                                                                   >  7:1235144/1‑146 (MQ=11)
                    tGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGGCATTCATGGTTGTGCGCCGCCAAAAGCAAAAACCCGCCG‑CAG‑CGGGgttt                                                                                                                  >  3:1289681/1‑151 (MQ=11)
                     cccccGCCACAAGCAAACCCCCGCCGACGCGGGTTTTTACGTAAACCACGGGAAACTGACCGATACGACGCTTGCTTTTGGGGATACGCCCGTGGACAGTCATTCCTCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CGGGttt                                                                                                                   <  5:265041/148‑1 (MQ=11)
                      ccccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTAGGGTATAGCGTCGTGTCGAGGCCATCAATATTTAGCACCAACAAAAACAAAAACCCGCCT‑CAG‑GGCGttttt                                                                                                                 >  6:302171/1‑150 (MQ=2)
                      ccactcaacaatcacaaaCCCGCCGCACCGGGTTATTACGTAAAGCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTa                                                                                                                <  5:564939/136‑1 (MQ=11)
                            cAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCTTGGCAAGTCAATCATGTTTGTGCCCCAGCAAAACCAAAAACCCGCCG‑CAgaccgttgtttaggaa                                                                                                            >  4:810386/1‑135 (MQ=11)
                            cAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGAAAGTCCTTCAAAGTAGAGCCCCAACAAAAGCAAAAACCCGCGG‑AAG‑AGGGGTTTTTCGTaa                                                                                                           >  6:1680638/1‑150 (MQ=11)
                            cAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGGATAGCGTCGTGGAGAGTCCGTGATGGTTTGGCCCCGGCAAAAGCAAAAACCCGCCG‑CAG‑gggggtgtttagtta                                                                                                           >  3:232142/1‑139 (MQ=11)
                                   aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATAGTAGTGCCACTCAAAAACCAAAAAAACGCCC‑AAC‑CGGGTGATTAATTAAATCATGtc                                                                                                   >  6:758756/1‑150 (MQ=11)
                                    aaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCGAGTCCATCATGGTTGGGCGCCGGCAAAACCAAAAACCCGCCG‑CAG‑GCGGGTTTTACggtaatgaagggc                                                                                                  >  8:1447946/1‑139 (MQ=2)
                                      accccGCCCAACCGCGCTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTTTGTATAGGGTCGTGGACAGTCATTCATATTTCTGCACCTCCAAAAGCAAAAAGACGCCG‑AAG‑CGTGTTTTTACGTAAATCAGGTGaaa                                                                                                <  4:418430/149‑1 (MQ=11)
                                      aagccgccgACGCTGGTTTTTACTTTAACGATGACTAGCTGACATATTAGCTGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGaaa                                                                                                <  5:877015/148‑1 (MQ=11)
                                       aCCCGCCGAAGCGGGTTTTTACGTAAACCANNTGAAANTGACCGANAAGCCGCTTTCTNTTGNGTNTANCGTCNNGGACANGNCTTACTATTTGNGCCCCGCCAAAACCAAANACCCGCCG‑NAG‑CGNGGTTNTTCGTTAATCacgggcaac                                                                                               >  5:603104/1‑143 (MQ=2)
                                       aCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCGAGTGCTTCATAGTTGGGCGGCGGCAAAAGCAAAAACCCGGCG‑CAG‑GGCGGTTTTACGTTAATCAggggcaa                                                                                                >  7:1131947/1‑145 (MQ=2)
                                         ccgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAATCATAGTTAAGCACCTACAAAAGCAAAAACCCGCCG‑AAG‑AGCGGTGTTACGTAAATCAGGTGaaaatt                                                                                             >  1:1301871/1‑148 (MQ=11)
                                             cgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCAAGTCATTCATCATTCTGCGCCGGCAAAAGCAAAAACCCGCGG‑CAG‑CCCGGTTTTACCGTAATGAGCGGCAACTGAccc                                                                                         >  7:518264/1‑150 (MQ=11)
                                                 gCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGTCAATCAAGATAGATCCCCGGCAAAAACAAAAACCCGCCG‑CAG‑AAGGTGTTTAAGGAAATCAGGGGaaaattgcccata                                                                                      >  2:1356871/1‑140 (MQ=2)
                                                   gggTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGAGGAAAGTCAATCAAGGTTAAACACCAACAAAAACAAAAACCCGCAG‑AAGACGGGTGTTAAGGT‑AATGAGGTGaaaattagccataatc                                                                                   >  1:1567078/1‑138 (MQ=2)
                                                      tttttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGTCAATCAAAGTGGAGCACCAACAAAACCAAAAACCCGCCGCAGG‑CGGGGTTTAAC‑TTAATCAGGGGAAAATTGCCGATAAGACgg                                                                                >  4:735278/1‑151 (MQ=2)
                                                         ttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTGTGCCCCGGCAAAACCAAAAACCCGCCG‑CAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAATCCGGGtt                                                                             >  7:155504/1‑151 (MQ=14)
                                                          tACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATCTTGAGGCCCCTGCAAAAGCAAAAACCCGCCG‑CAG‑CGGGTTTTTAAGGAAATGAGGTGCAACTTCCCGCTAATCCGGGttg                                                                            >  7:330409/1‑150 (MQ=14)
                                                           aCGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTAGTGGGGAGTCCATCAAAATTGAGCCCCGGCAAAACCAAAAACCCGCCA‑CAG‑GGGGGTTTTACGGTAATCAGGTGAAAGTTGCCGATAAGCCGGGTTCt                                                                           >  2:212592/1‑151 (MQ=9)
                                                               aaaCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAGAGTCAATCAAAATTGGGCCCCAACAAAAGCAAAAACCCGCCG‑AAG‑AGGGTGTTTAAGTTAATCAGGGGAAACTGCCCCATCAGCCGGGTTCTGtgg                                                                       >  4:331524/1‑149 (MQ=14)
                                                                   cAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATCTTTGTGCCCCGCCAAAAGCAAAAACCCGCCG‑CAG‑CGGGGTTTTACGTTAATCACGGGAAAATTACCCCTAACCCGGGTTCTGtggtggc                                                                   >  7:1652292/1‑150 (MQ=14)
                                                                    aGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATCTTTCGGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGAc                                                                  >  7:247682/1‑151 (MQ=255)
                                                                    aGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑c                                                                                                                         <  3:994666/96‑1 (MQ=17)
                                                                       tGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATATTTCTGCCCCTCCAAAACCAAAAACCCGCCG‑CAG‑CCGGGTTTTAAGTAAATCACGTGAAAATGACCCCTAAGCCGGGTTGTGtggtgggctgg                                                               >  5:1200730/1‑146 (MQ=14)
                                                                         aaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATCTTTGTGCCCCTCCAAAACCAAAAACCCGCCG‑CAG‑CGGGGTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGGGGACAGtcc                                                             >  5:1581096/1‑150 (MQ=14)
                                                                         aaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAATCATATTTATGCCCCAACAAAAGCAAAAAACCCCCG‑CAC‑CCGGGTTTTACGAAAATCAGGTGAAAATGACCCATAAACCGGGTTCTGTCGTggggccgc                                                              >  1:40788/1‑144 (MQ=14)
                                                                          aaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATGTTTGTGCCCCAACAAAACCAAAAACCCGCCG‑AAG‑CCCGGTTTTACGTAAATCAGGTGAAAATTACCCATAAGCCGGGTTATgggcggggccctcct                                                            >  7:444245/1‑137 (MQ=11)
                                                                          aaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTGTGCCCCTCCAAAACCAAAAACCCCCCG‑AAG‑CGGGGTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtcat                                                            >  3:510752/1‑151 (MQ=255)
                                                                             tGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTACAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTAGTGGACAGtcatta                                                          >  6:1130864/1‑149 (MQ=255)
                                                                              gACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTAAGTAAATCAGGTGAAACTGCCCGATAAGCCGGGTTCTGTCGTGGACAGtcattcat                                                        >  3:34766/1‑151 (MQ=255)
                                                                              gACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTATGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTACCTAAATAACGTGAAACTGACCGATAAGCCGGGTTCTGTAGTGGACAGtgattaa                                                         >  4:1552936/1‑148 (MQ=14)
                                                                              atccGATAAGCCGCTTTCTTTTGGGTATATTGTCTTGGACAGTCATTCATCTTTCTGCCCCTCCAAAATCAAAAACCCGCCG‑AAT‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtcattca                                                         <  1:1003478/148‑1 (MQ=17)
                                                                               aCCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTTTACTGTCATTCATCTTTCTTCCCCTCAAAAAGAAAAAACCCGCCG‑AAG‑CGTGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGt                                                               <  4:652178/143‑1 (MQ=14)
                                                                                ccGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTATGCCCCTCCAAAAGCAAAAACCCGCCG‑CAC‑CGGGGTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCt                                                      >  1:694934/1‑151 (MQ=255)
                                                                                ccGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCAAATTTCTGGCCCACCAAAAGCAAAAAACCCCCG‑CAC‑CGGGTTTTTTCGTAAATCCAGTGAAACAGACCCATAAGCCGGGTTCTGGGGTGGGCAGtccataatct                                                      >  1:1231141/1‑143 (MQ=14)
                                                                                    tAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTGTGCCCCGCCAAAAGCAAAAAACCGCCG‑CAG‑CGGGGTTTTACGTAAATGAGGTGAAACTGCCCGATAAGCCGGGTTGTGTCGTGGACAGTCATTCAtatatgg                                                  >  3:1641526/1‑148 (MQ=17)
                                                                                    tAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTAATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGAACGAAAAGCCGGGTTATGTCGTGGACAGTAATTCATCTAGGc                                                  >  3:968394/1‑151 (MQ=255)
                                                                                     aaGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCAACTTTCTGGCCCACCAAAAGCAAAAAACCGCCG‑AAC‑CGGGGGTTTACCTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACCGtaattaaaatacgcc                                                 >  8:1308756/1‑141 (MQ=14)
                                                                                        ccGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAACCAAAAACCCGCCG‑AAG‑CGGGTTTTTAAGTAAATAAGGTGAAACTGACCGATAAGCCGGGTTCTGTGGTGGCCAGTCATTCAACTAGGCCAGc                                              >  7:1059414/1‑151 (MQ=17)
                                                                                          cccttcttaGGGGTATAGCGTCGTGGACAGTCATGCATCATTCTCCCCCTCCAAAAGCCAAAACGCGCGT‑AAG‑CGGGTTTTTACTTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCaa                                            <  5:48302/148‑1 (MQ=18)
                                                                                           ctttctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTTGTGCCCCTACAAAACCAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCCATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCaa                                            >  6:338198/1‑150 (MQ=255)
                                                                                           cctgctttTGGGTAAAGCGTCTTTTAAAGACATACAACATTCACGCCCTCCACCAGCAAACAGACGGCT‑CAG‑CTGGCTTTTACTTAAACCAGGTTAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAAt                                           <  7:192733/147‑1 (MQ=255)
                                                                                            tttctttTGGGTATAGCGTCGTGGCCAGTGATTGATCTTTCTGCCCATACAAAAGCAAAAAACCCCCG‑AAG‑CGGGTTTTTACGTAAATGAGGTGAAAATTCCCCAAAAGCCCGGTGGTGTGGTGGACAGTCATTCATCTAGGCCAGGAAtg                                          >  3:1251657/1‑150 (MQ=255)
                                                                                            tttctttTGGGTATAGCGTCGTGGACAGTCATTGATCTTTCTGCCCCGACAAAAGCAAAAACCCGCCG‑CAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTGGTGGGCAGTCATTCATCTAGGCCAGCAATc                                          >  2:505172/1‑151 (MQ=18)
                                                                                            tttctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑CAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGCCCGATAAGCCGGGTTCTGTCGTGGCCAGTCATTCATCTAGGCCAGCAATc                                          >  3:42185/1‑151 (MQ=255)
                                                                                            tttctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGACACTGCCCCATAAGCCGGGTTATGTCGTGGACAGTGATTCATCTAGGCCAGCAATc                                          >  2:930246/1‑151 (MQ=255)
                                                                                            tttctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGAAAATGACCGATAAACCCGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATc                                          >  1:1110088/1‑151 (MQ=255)
                                                                                             ttctttTTGGTATATTGTCTTTGAGATTCATTCATCTTTCAGCCACTCCAAAAGCAAAAACCCGCCG‑AAG‑TGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATc                                          <  1:1221740/150‑1 (MQ=18)
                                                                                             ttctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑Cggg                                                                                                                      <  3:612048/74‑1 (MQ=33)
                                                                                               ctttTGGGTATAGCGTCGTGGGCAGTCATTCATCTTTATGCCCCGCCAAAAGCAAAAAACCGCCG‑AAG‑CGGGTTTTTAAGTTAATCACGTGAAACTTACCGAGAAGCCGGGGTCTGTCGTGGACAGTGATTAATATAGGCCAGCAATAGcg                                       >  1:880645/1‑150 (MQ=255)
                                                                                                ttttGGGTATAGCGTCGTGGACAGTGATTGATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTGGTGGCCAGTCATTCATCTAGGCCAGCCATCGCTc                                      >  7:323762/1‑151 (MQ=255)
                                                                                                ttttGGGTATAGCGTCGTGGACAGTAATTGATATTTATGCCCCAACAAAAACAAAAAACCCCCG‑AAG‑AGGGTTTTTAAGTAAATAAGGTTAAAAAGAACGATAATCCGGGTTATGTCGTGGGAAGTCATTAATCTAGGCCAGCAATAGCTc                                      >  2:277899/1‑151 (MQ=255)
                                                                                                 tttGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAACCAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTc                                      <  8:757958/150‑1 (MQ=255)
                                                                                                 tttGGGTATAGCGTCGTGGACAGTCATTAATCTTTGTGCCCCTACAAAAACAAAAAACCGCCG‑AAG‑CGGGTTTTTAAGTAAATCAGGTGAAACTGACCGAAAAGCCGGGTTATGTCGTGGGCAGTCATTAATCTAGGCCAGCAATAGCTc                                      >  4:1674090/1‑150 (MQ=255)
                                                                                                  ttGGGTATAGCGTCGTGGACAGTCATTCATATTTCTGCCCCTACAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGAAAATGACCGATAACCCCGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATAGCTCAc                                    >  8:1365519/1‑151 (MQ=18)
                                                                                                   tGGGTATAGCGTCGTGGACAGTCATTAATCTTTATGCACCTCCAAAAACAAAAACCCCCCC‑AAG‑CGGGTTTTTAATTAAATCAGGTGAAACATACCGAAAAGCCGGGTTATGTCGTGGACAGTCATTAAAATAGGCCAGCaaaccataac                                    >  1:1094574/1‑142 (MQ=255)
                                                                                                   tGGGTATAGCGTCGTGGAAAGTAATTGATATTTCTTCCCCTACAAAAGAAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCATGTGAAACTTACCGATAAGCAGGGTTATGTCGTGGACAGTGATTCATCTAGGCCAGCCATCGCtacat                                   >  6:981640/1‑147 (MQ=18)
                                                                                                     ggTATAGCGTCGTGGACAGTCATTGATCTTTCTGCCCCTCCAAAAGCAAAAACCCCCCG‑AAC‑CGGGTTTTTAAGTAAATAAGGTGAAACTGACCGATAAGCCCGGTTCTGTCGTGGACAGTCATTACTCTATGCCAGCCATCGCTCACAgg                                 >  2:802549/1‑151 (MQ=18)
                                                                                                       tataGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATAAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTAATATAGGCCAGCAATCGCTCACTGGCt                               >  5:1619381/1‑151 (MQ=255)
                                                                                                       tataGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGCCAGTCATTCATCTAGGCCAGCAATCGCTACCTGGcg                               >  2:1563104/1‑150 (MQ=255)
                                                                                                           gCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCa                             >  5:1371793/1‑149 (MQ=255)
                                                                                                            ggtcgtGTAGACTCATTCATCTTGCAGCCCCTCCACAAGCAACACCCCGCCG‑AAG‑CGTGTTTTTAGGTAAATCACGAGAACCTGACCGATAAGCCGGCTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagc                          <  3:1051196/150‑1 (MQ=21)
                                                                                                            cgtcgtGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagc                          >  6:1603020/1‑151 (MQ=255)
                                                                                                            cgtcgtGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagc                          >  3:1612396/1‑151 (MQ=255)
                                                                                                             gtcgtGGACAGGCATTCCTCTTTCTGCCCCTCCCAAAGCACACACGCGCCG‑TCG‑CGGGTTTTTACTTAACTCCGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACGGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagca                         <  8:486106/151‑1 (MQ=21)
                                                                                                                gtGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGcc                      >  6:670166/1‑151 (MQ=255)
                                                                                                                gtGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGcc                      >  7:89713/1‑151 (MQ=255)
                                                                                                                  ggtcAGTCAATCCTCTTTCAGACAATGAAAAAGCATAAACCCCTAG‑AAG‑AGGGTTTTTAGTTATATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTa                    <  6:1204681/148‑1 (MQ=25)
                                                                                                                  ggactttctttcttcTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTa                    <  6:146484/142‑1 (MQ=255)
                                                                                                                  ggACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCt                     >  8:1143851/1‑150 (MQ=255)
                                                                                                                   gACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTAc                   >  1:235987/1‑151 (MQ=255)
                                                                                                                    aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTAcc                  >  3:574355/1‑151 (MQ=255)
                                                                                                                     cAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagcag                        <  7:1226790/144‑1 (MQ=255)
                                                                                                                     cAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTAccc                 >  8:128248/1‑151 (MQ=255)
                                                                                                                     cAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTAccc                 >  5:1419508/1‑151 (MQ=255)
                                                                                                                      aGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCa                                     <  8:1406586/130‑1 (MQ=255)
                                                                                                                        tcattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCggg              >  1:1674510/1‑151 (MQ=255)
                                                                                                                         cattcatCTTTCAGCCCCTCCAAAACCAAAACGCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCggg              <  3:749127/150‑1 (MQ=255)
                                                                                                                          attcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCt                               <  8:1115446/132‑1 (MQ=255)
                                                                                                                            tcatCTTTCAGCCCCTCCAAATGCAAACACCCGCCG‑AAG‑TGGTTTTTTACGTTAATCAGGTGAAACTTACGGATTATCCGGTTTGTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTc           <  8:199029/150‑1 (MQ=25)
                                                                                                                             catCTTTCTGCCACTAAAAAATCAAAAACCAGGCT‑AAG‑CGTGTTTTTACGTAAATCTTGTTAATCTGACCGATAAGGCGTGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCa          <  6:870614/150‑1 (MQ=37)
                                                                                                                              atatttCTGACCCTCAAAAATCAAAAACCAGCAT‑AAG‑CGTGTTTTTACGTAAATCATTTGAATCTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAg         <  1:961310/147‑1 (MQ=255)
                                                                                                                               ccTTGCTGCCCCTCCAAAAGCACAACCCCGCAG‑ACG‑CGGGTTTTTACGTACACCAGGTGACACTGCCCGATTAGCCGGCTGCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTa       <  7:471714/150‑1 (MQ=25)
                                                                                                                                 tttCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTTCg     >  1:114338/1‑151 (MQ=255)
                                                                                                                                 gttCAGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACg     <  8:302615/150‑1 (MQ=255)
                                                                                                                                  ttCTGCCCCTCCAAAAGCAAAAACCCTCCG‑ACG‑AGGGGTTTTACGTAAATCAGGTGAATCTGACCGATAAGCCGGCTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGt        <  7:55354/147‑1 (MQ=37)
                                                                                                                                  ttCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACgg    >  4:976639/1‑151 (MQ=255)
                                                                                                                                  ttCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACg     <  5:946843/150‑1 (MQ=255)
                                                                                                                                   tCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACggg   >  8:705394/1‑151 (MQ=255)
                                                                                                                                    cTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGc  >  7:1329976/1‑151 (MQ=255)
                                                                                                                                    |                                                                                                                                                        
TGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCG‑AAG‑CGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGC  >  NC_000913/3270035‑3270317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑