Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,903 G→A E523K (GAA→AAA)  thrA → Bifunctional aspartokinase/homoserine dehydrogenase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,9030GA98.0% 1399.9 / 6.5 404E523K (GAA→AAA) thrABifunctional aspartokinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base G (5/3);  new base A (198/198);  total (203/201)
Fisher's exact test for biased strand distribution p-value = 7.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 409 total aligned reads displayed.

CGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTT  >  NC_000913/1755‑2050
                                                                                                                                                    |                                                                                                                                                   
    ggCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAAc                                                                                                                                               >  4:1280590/1‑151 (MQ=255)
    ggCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAAc                                                                                                                                               >  8:1404221/1‑151 (MQ=255)
     gCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACt                                                                                                                                              <  1:186284/151‑1 (MQ=255)
     gCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACt                                                                                                                                              <  1:746922/151‑1 (MQ=255)
            cGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCaa                                                                                                                                       <  1:277239/151‑1 (MQ=255)
             ggTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAg                                                                                                                                      <  4:140017/151‑1 (MQ=255)
                gcgcTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGAAAGcc                                                                                                                                    <  8:1071093/150‑1 (MQ=255)
                 cgcTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCa                                                                                                                                   >  6:919014/1‑150 (MQ=255)
                  gctgctGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCaa                                                                                                                                  >  2:933181/1‑150 (MQ=255)
                  gctgctGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATAACGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTCGCAGAAAGAACTGGCGCAAGCCaa                                                                                                                                  >  1:1108517/1‑150 (MQ=255)
                    tgctgGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCaa                                                                                                                                  >  4:1007526/1‑148 (MQ=255)
                         gAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGt                                                                                                                          <  1:1437209/151‑1 (MQ=255)
                            cAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTa                                                                                                                       <  2:138413/151‑1 (MQ=255)
                             aaCTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTa                                                                                                                       >  3:1056562/1‑150 (MQ=255)
                              aCTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAAt                                                                                                                     >  7:368119/1‑151 (MQ=255)
                              aCTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAAt                                                                                                                     <  5:1567045/151‑1 (MQ=255)
                              aCTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAAt                                                                                                                     <  7:748685/151‑1 (MQ=255)
                               cTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAAtc                                                                                                                    >  3:198438/1‑151 (MQ=255)
                                 gAAGCGTCAGCAAAGCTGGCTGAAGAATAAAGATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAAtctc                                                                                                                  <  2:612518/151‑1 (MQ=255)
                                 gAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGCCGCAAGCCAAAGAGCCGTTTAAtc                                                                                                                    <  8:1292336/149‑1 (MQ=255)
                                  aaGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCg                                                                                                                 >  8:1184458/1‑151 (MQ=255)
                                  aaGCGTCAGCAAAGCTGGATGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAACGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAATACTGGCAGAAAGAACTTACGAAAGCCAAAGAGCCGTTTAATCTCg                                                                                                                 >  2:1450813/1‑151 (MQ=255)
                                  aaGCGTCACCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCg                                                                                                                 <  6:246116/151‑1 (MQ=255)
                                     cGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGgc                                                                                                              >  2:1107918/1‑151 (MQ=255)
                                        cAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGgcgc                                                                                                            <  4:854919/150‑1 (MQ=255)
                                          gCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCtt                                                                                                          >  2:399833/1‑150 (MQ=255)
                                            aaaGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAAt                                                                                                       <  1:1618728/151‑1 (MQ=255)
                                            aaaGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAAt                                                                                                       <  5:440149/151‑1 (MQ=255)
                                                ctggctgAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGc                                                                                                   >  7:1489441/1‑151 (MQ=255)
                                                  ggctgAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATATGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCTCCt                                                                                                 >  4:1564551/1‑151 (MQ=255)
                                                   gctgAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAAGTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTc                                                                                                >  4:541275/1‑151 (MQ=255)
                                                      gaagaaTAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTg                                                                                             >  7:688030/1‑151 (MQ=255)
                                                        agaaTAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGaa                                                                                           <  6:725450/151‑1 (MQ=255)
                                                           aTAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGaa                                                                                           >  3:1126316/1‑148 (MQ=255)
                                                           aTAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGaa                                                                                           >  2:433808/1‑148 (MQ=255)
                                                           aTAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGaa                                                                                           >  1:38011/1‑148 (MQ=255)
                                                           aTAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCGTAATTCGCCTCGTGaa                                                                                           >  8:586791/1‑148 (MQ=255)
                                                            tAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGa                                                                                        >  7:1392496/1‑150 (MQ=255)
                                                             aaaCATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGaa                                                                                       <  5:1324295/150‑1 (MQ=255)
                                                              aaCATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAatt                                                                                     >  1:108689/1‑150 (MQ=255)
                                                              aaCATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAat                                                                                      >  3:15753/1‑150 (MQ=255)
                                                              aaCATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAat                                                                                      >  1:1376630/1‑150 (MQ=255)
                                                                cATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCACGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATatc                                                                                   <  1:978033/151‑1 (MQ=255)
                                                                cATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATatc                                                                                   >  8:267650/1‑151 (MQ=255)
                                                                aatatCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATatc                                                                                   <  4:351965/150‑1 (MQ=255)
                                                                 atatCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATatca                                                                                  <  5:348287/151‑1 (MQ=255)
                                                                  tatCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATatcat                                                                                 >  6:388130/1‑151 (MQ=255)
                                                                   atCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATatcatc                                                                                >  5:537658/1‑151 (MQ=255)
                                                                     cGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATctg                                                                              >  2:469916/1‑151 (MQ=255)
                                                                     cGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATct                                                                               <  5:230899/150‑1 (MQ=255)
                                                                     cGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATct                                                                               <  4:431755/150‑1 (MQ=255)
                                                                      gACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATctgc                                                                             <  5:899446/151‑1 (MQ=255)
                                                                          tACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGa                                                                          <  8:705539/150‑1 (MQ=255)
                                                                           aCGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAAc                                                                        <  5:1106262/151‑1 (MQ=255)
                                                                            cGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAAcc                                                                       >  1:910333/1‑151 (MQ=255)
                                                                               gtCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCgg                                                                    >  5:717947/1‑151 (MQ=255)
                                                                                  tGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTc                                                                  >  4:1146771/1‑150 (MQ=255)
                                                                                   gCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGt                                                                   <  3:390063/148‑1 (MQ=255)
                                                                                   gCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTc                                                                  <  8:1528329/149‑1 (MQ=255)
                                                                                    nnnnnnnnccANNTNNANGGNTCTGCTCACCAANGTACATGGCCTTAATNTGGAAAACTGGCAGNAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGNATATCATCTGCTGAACCCGGTCAtt                                                               <  3:277600/143‑1 (MQ=255)
                                                                                    cGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCAtt                                                               <  2:1670188/151‑1 (MQ=255)
                                                                                       tgtTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCAttgtt                                                            >  8:771922/1‑151 (MQ=255)
                                                                                           gCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACt                                                        >  4:918311/1‑151 (MQ=255)
                                                                                           gCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACt                                                        <  6:101719/151‑1 (MQ=255)
                                                                                           gCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACt                                                        <  6:1287821/151‑1 (MQ=255)
                                                                                              aaCTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGc                                                      >  4:1104282/1‑150 (MQ=255)
                                                                                              aaCTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCa                                                     <  1:1210267/151‑1 (MQ=255)
                                                                                                cTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACt                                                   >  3:888411/1‑151 (MQ=255)
                                                                                                 tCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACtt                                                  >  2:1226848/1‑151 (MQ=255)
                                                                                                   gAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTcc                                                >  2:644942/1‑151 (MQ=255)
                                                                                                    aaGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTcca                                               <  6:1629316/151‑1 (MQ=255)
                                                                                                       gCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTccagcc                                            <  6:1519966/151‑1 (MQ=255)
                                                                                                         tctGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTccagcc                                            >  7:1627414/1‑149 (MQ=255)
                                                                                                           tGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGc                                        >  7:886959/1‑151 (MQ=255)
                                                                                                            gCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTccagccag                                          <  2:1323538/148‑1 (MQ=255)
                                                                                                               cACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCCAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTg                                    <  6:1391358/151‑1 (MQ=255)
                                                                                                               cACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGt                                     <  3:1467903/150‑1 (MQ=255)
                                                                                                               cACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTg                                    <  5:1203916/151‑1 (MQ=255)
                                                                                                               cACCAATGTACATGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATATCGGGCGCTTAATTCGCCGCGTGAAAGAATATCATCTGCTGAACCCGGTGATTGTTGACTGCACTTCCAGCCACGCACTgg                                   >  5:1501167/1‑151 (MQ=255)
                                                                                                                aCCAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTgg                                   >  2:303669/1‑151 (MQ=255)
                                                                                                                 ccAATGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGc                                  >  1:10253/1‑151 (MQ=255)
                                                                                                                   aaTGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCgg                                >  7:283314/1‑151 (MQ=255)
                                                                                                                   aaTGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCgg                                >  1:642863/1‑151 (MQ=255)
                                                                                                                    aTGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGACCCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCggg                               >  4:19490/1‑150 (MQ=255)
                                                                                                                    aTGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGa                               <  7:527383/151‑1 (MQ=255)
                                                                                                                    aTGTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGa                               <  2:1606981/151‑1 (MQ=255)
                                                                                                                      gTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATc                             >  7:120126/1‑151 (MQ=255)
                                                                                                                        aCATGGCCTTAATCTGGAAAACTGTCATAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCaa                           <  3:1284386/151‑1 (MQ=255)
                                                                                                                         cATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAat                          <  4:750491/151‑1 (MQ=255)
                                                                                                                               cTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCg                    >  3:14182/1‑151 (MQ=255)
                                                                                                                                 tAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGa                   <  8:333022/150‑1 (MQ=255)
                                                                                                                                  aaTCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACt                 >  3:482735/1‑151 (MQ=255)
                                                                                                                                  aaTCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACt                 >  8:1565229/1‑151 (MQ=255)
                                                                                                                                  aaTCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACt                 <  3:747383/151‑1 (MQ=255)
                                                                                                                                       ggAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCt             <  6:1154237/150‑1 (MQ=255)
                                                                                                                                       ggAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTg            <  8:193017/151‑1 (MQ=255)
                                                                                                                                          aaaCTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTgcgc         >  1:94769/1‑151 (MQ=255)
                                                                                                                                            aCTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGcgcg        >  6:562270/1‑150 (MQ=255)
                                                                                                                                             cTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGa       >  4:617537/1‑150 (MQ=255)
                                                                                                                                                 cAGAAAGAACTCGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCTCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCACCCAGGCAGTGGCGGATCAATATGCCGACTTCCAGCGCGAAGGtt  <  4:1499525/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTT  >  NC_000913/1755‑2050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: