Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,817,197 G→A R103C (CGC→TGC)  yqaB ← fructose‑1‑P and 6‑phosphogluconate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,817,1970GA97.1% 1394.3 / 19.1 415R103C (CGC→TGC) yqaBfructose‑1‑P and 6‑phosphogluconate phosphatase
Reads supporting (aligned to +/- strand):  ref base G (4/8);  new base A (200/203);  total (204/211)
Fisher's exact test for biased strand distribution p-value = 3.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.64e-01

Only 100 of 430 total aligned reads displayed.

GCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAAT  >  NC_000913/2817049‑2817344
                                                                                                                                                    |                                                                                                                                                   
 cgcACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGcaacg                                                                                                                                                  >  6:1066713/1‑151 (MQ=255)
  gcACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGcaacgc                                                                                                                                                 >  6:333751/1‑150 (MQ=255)
   cacaACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCaacgac                                                                                                                                                >  8:796438/1‑151 (MQ=255)
    acaacaAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGAcc                                                                                                                                               <  2:1224010/151‑1 (MQ=255)
       acaaAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGGGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATg                                                                                                                                            <  1:1077757/151‑1 (MQ=255)
         aaaaaTGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATggc                                                                                                                                          >  6:1191709/1‑149 (MQ=255)
         aaaaaTGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGcc                                                                                                                                          >  8:655552/1‑151 (MQ=255)
         aaaaaTGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGcc                                                                                                                                          >  8:1045719/1‑151 (MQ=255)
          aaaaTGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGcc                                                                                                                                          >  6:158798/1‑150 (MQ=255)
           aaaTGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCaa                                                                                                                                        <  7:605310/151‑1 (MQ=255)
             aTGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACt                                                                                                                                      >  4:1610222/1‑151 (MQ=255)
                 gtCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTc                                                                                                                                  <  4:1180888/151‑1 (MQ=255)
                     ggCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTcacca                                                                                                                              <  3:28706/151‑1 (MQ=255)
                     ggCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTcac                                                                                                                                <  7:831525/149‑1 (MQ=255)
                       cgcgGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTcaccac                                                                                                                             >  1:1021283/1‑150 (MQ=255)
                         cgGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATc                                                                                                                          <  6:890419/151‑1 (MQ=255)
                            gTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATcaac                                                                                                                       <  3:135420/151‑1 (MQ=255)
                               tATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATcaacaac                                                                                                                    >  1:341768/1‑150 (MQ=255)
                                aTGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAaga                                                                                                                   <  5:840170/151‑1 (MQ=255)
                                  ggTGATTGACGTGACCGCCAGAGACGACGGCGTCAAAATAATCGCGTAATCCCACGTCCGCCAGCAATGCCTCACCGATGTCGCTTTCACTCCCCCTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAagag                                                                                                                  <  4:538968/150‑1 (MQ=255)
                                   gtgtTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGa                                                                                                                <  6:732405/151‑1 (MQ=255)
                                    tgtTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAagagca                                                                                                                >  7:1104114/1‑148 (MQ=255)
                                       ttGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGc                                                                                                             <  6:24759/150‑1 (MQ=255)
                                       ttGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCg                                                                                                            >  1:843941/1‑151 (MQ=255)
                                         gACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCgg                                                                                                           <  5:315443/150‑1 (MQ=255)
                                          aCGTGATCGGCGGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGtt                                                                                                         >  2:165452/1‑151 (MQ=255)
                                           cGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTc                                                                                                        >  8:905806/1‑151 (MQ=255)
                                            gTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCATCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCAGATCAACAAGAGGAAGCGGTTCg                                                                                                       <  3:864672/151‑1 (MQ=255)
                                            gTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCg                                                                                                       <  5:1671341/151‑1 (MQ=255)
                                            gTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCg                                                                                                       <  3:969956/151‑1 (MQ=255)
                                              gATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGCCCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGAc                                                                                                     >  2:1105665/1‑151 (MQ=255)
                                                 cGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCt                                                                                                  >  1:1581264/1‑151 (MQ=255)
                                                     aGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATcc                                                                                              <  3:1135660/151‑1 (MQ=255)
                                                        gacgacGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCcagc                                                                                           >  2:331369/1‑151 (MQ=255)
                                                         acgacgGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCcagca                                                                                          <  5:512818/151‑1 (MQ=255)
                                                              ggCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGAGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATa                                                                                     <  7:1363350/151‑1 (MQ=255)
                                                               gCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATAc                                                                                    <  7:1666567/151‑1 (MQ=255)
                                                                cGTCAAAATAATGGCGTAATCCCAGCTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTGCGACGCTATCCAGCAGCATAct                                                                                   <  2:644579/151‑1 (MQ=255)
                                                                 gTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATActt                                                                                  <  1:488723/151‑1 (MQ=255)
                                                                 gTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATActt                                                                                  <  7:1194066/151‑1 (MQ=255)
                                                                 gTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATActt                                                                                  >  5:1430457/1‑151 (MQ=255)
                                                                 gTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATActt                                                                                  >  4:90662/1‑151 (MQ=255)
                                                                   cAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATActtct                                                                                >  5:347751/1‑151 (MQ=255)
                                                                    aaaaTAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATActtctt                                                                               >  1:681380/1‑151 (MQ=255)
                                                                         atttgCGTAATGGCAGGTGCGCCAGCTATTCCTCTGCGATGGCTCTTTCAGTCCCCTTTCCTACAGCCTTTGTGAAACGACCAGGCCAACTTTTCACCACATCAACATGAGGAAGGGGTTGGACGCTATCCAGCAGGATACTTCTTACTGc                                                                          <  5:1553469/147‑1 (MQ=255)
                                                                         aatGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTgg                                                                          >  8:1131110/1‑150 (MQ=255)
                                                                             gCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCt                                                                      >  1:794773/1‑151 (MQ=255)
                                                                              cGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTg                                                                     <  6:1350955/151‑1 (MQ=255)
                                                                               gTAATCCCAGGTGCGCCAGCAATGCCTCAGGGATGGCGCTTTCACTCCCCGGTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGt                                                                    <  1:1237714/151‑1 (MQ=255)
                                                                               gTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGt                                                                    <  6:655929/151‑1 (MQ=255)
                                                                               gTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGt                                                                    >  3:463236/1‑151 (MQ=255)
                                                                                 aaTCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGtt                                                                   <  8:351573/150‑1 (MQ=255)
                                                                                   tCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGttttt                                                                >  5:438700/1‑151 (MQ=255)
                                                                                     ccAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTc                                                              <  1:1290657/151‑1 (MQ=255)
                                                                                     ccAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTc                                                              >  1:1451008/1‑151 (MQ=255)
                                                                                       aGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCAc                                                            >  8:567446/1‑151 (MQ=255)
                                                                                       aGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCAc                                                            >  7:769718/1‑151 (MQ=255)
                                                                                         gTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCAcgc                                                          >  1:269524/1‑151 (MQ=255)
                                                                                             gcCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTa                                                      <  1:1655437/151‑1 (MQ=255)
                                                                                              ccAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTaa                                                     <  4:1107652/151‑1 (MQ=255)
                                                                                              ccAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTa                                                      >  5:272928/1‑150 (MQ=255)
                                                                                               cAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCTTTCCTACTGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCAGGCGCTAAc                                                    <  4:626419/151‑1 (MQ=255)
                                                                                               cAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAAc                                                    <  5:1224564/151‑1 (MQ=255)
                                                                                                aGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACg                                                   >  8:1194909/1‑151 (MQ=255)
                                                                                                 gCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGc                                                  <  4:1484697/151‑1 (MQ=255)
                                                                                                   aaTGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCAt                                                >  3:580454/1‑151 (MQ=255)
                                                                                                    aTGCCTGAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCTGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATg                                               <  3:161488/151‑1 (MQ=255)
                                                                                                     tGCCTCAGCGATGGCGCTTCCACTCCCCGTTCCTACAGCCATTGGGCAACGCCCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGc                                              >  7:285334/1‑151 (MQ=255)
                                                                                                      gCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGc                                              <  4:273989/150‑1 (MQ=255)
                                                                                                      gCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCg                                             <  7:1281256/151‑1 (MQ=255)
                                                                                                      gCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCg                                             >  3:72079/1‑151 (MQ=255)
                                                                                                         gcAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGt                                          <  2:813274/150‑1 (MQ=255)
                                                                                                          cAGCGATGGCGCTATCACTCCCCGTTCCTACAGCCATTCGGCAACCACCATGCCAACTTTTCACCACATCAACAACAGGAAGCGGTTCGACGATATACAGCAGCATACTTCATACTGCTACTGTTTTTTCAAGCGCTAACGCATGAGGGTc                                         >  4:1156731/1‑151 (MQ=255)
                                                                                                           cgCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCg                                        <  5:622846/150‑1 (MQ=255)
                                                                                                            gCGATGTCGCTTTCACTCCCCGTTCCTACACCCATTGGGCAACGACCATGCGAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCga                                       <  2:46136/151‑1 (MQ=255)
                                                                                                             cGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGACGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCgag                                      <  2:1457018/151‑1 (MQ=255)
                                                                                                              gATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCg                                        >  7:1429476/1‑148 (MQ=255)
                                                                                                                tGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCg                                        >  4:478123/1‑146 (MQ=255)
                                                                                                                 ggCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCg                                  <  3:515973/151‑1 (MQ=255)
                                                                                                                 ggCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCg                                  <  8:1362227/151‑1 (MQ=255)
                                                                                                                   cgcTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGc                                <  4:786034/151‑1 (MQ=255)
                                                                                                                    gcTTTCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGcc                               >  7:902395/1‑151 (MQ=255)
                                                                                                                      tttCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTg                             >  5:134572/1‑151 (MQ=255)
                                                                                                                       ttCACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTg                             >  8:1600239/1‑150 (MQ=255)
                                                                                                                         cACTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGAt                           <  7:1571085/150‑1 (MQ=255)
                                                                                                                          aCTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTc                         >  3:409896/1‑151 (MQ=255)
                                                                                                                          aCTCCCCGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTc                         <  1:1377232/151‑1 (MQ=255)
                                                                                                                               ccGTTCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTc                    >  7:405954/1‑151 (MQ=255)
                                                                                                                                  ttCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCaat                 >  5:917899/1‑151 (MQ=255)
                                                                                                                                   tCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCaat                 <  4:982823/150‑1 (MQ=255)
                                                                                                                                   tCCTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTCATTCAGCTCaat                 >  1:1163418/1‑150 (MQ=255)
                                                                                                                                    ccTACAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGACTCAGCTCaataa               <  2:1580524/151‑1 (MQ=255)
                                                                                                                                        cAGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGc           <  2:1533608/151‑1 (MQ=255)
                                                                                                                                         aGCCATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGcc          >  2:919017/1‑151 (MQ=255)
                                                                                                                                           ccATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTg        <  6:452367/151‑1 (MQ=255)
                                                                                                                                            cATTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGGTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGa       <  3:1472978/151‑1 (MQ=255)
                                                                                                                                             aTTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTg        >  3:666218/1‑149 (MQ=255)
                                                                                                                                             aTTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAg      <  3:804502/151‑1 (MQ=255)
                                                                                                                                             aTTGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAg      <  1:419555/151‑1 (MQ=255)
                                                                                                                                               tGGGCAACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGc     >  5:1640674/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAAT  >  NC_000913/2817049‑2817344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: