Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,061,916 C→T A356V (GCC→GTC)  scpA → methylmalonyl‑CoA mutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,061,9160CT96.6% 1358.5 / 24.2 411A356V (GCC→GTC) scpAmethylmalonyl‑CoA mutase
Reads supporting (aligned to +/- strand):  ref base C (4/10);  new base T (196/198);  total (200/211)
Fisher's exact test for biased strand distribution p-value = 1.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.37e-01

Only 100 of 412 total aligned reads displayed.

ACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAAT  >  NC_000913/3061767‑3062063
                                                                                                                                                     |                                                                                                                                                   
     aCCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGa                                                                                                                                               <  8:1008966/151‑1 (MQ=255)
       ccGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACg                                                                                                                                             <  6:1121460/151‑1 (MQ=255)
         gAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGa                                                                                                                                            >  3:427341/1‑150 (MQ=255)
          aaaTCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAg                                                                                                                                          <  6:544492/151‑1 (MQ=255)
             tCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAgcgc                                                                                                                                       >  3:1487033/1‑151 (MQ=255)
               aCTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCtt                                                                                                                                     <  6:132893/151‑1 (MQ=255)
                cTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTg                                                                                                                                    >  5:1294190/1‑151 (MQ=255)
                 tGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTgg                                                                                                                                   >  4:1041201/1‑151 (MQ=255)
                  ggCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTg                                                                                                                                    <  7:837837/149‑1 (MQ=255)
                    cgcTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGttt                                                                                                                                <  1:664328/151‑1 (MQ=255)
                     gcTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTg                                                                                                                               >  1:255272/1‑151 (MQ=255)
                        gCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGc                                                                                                                              <  2:346207/149‑1 (MQ=255)
                         cGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTa                                                                                                                           <  8:1394753/151‑1 (MQ=255)
                          gTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTAc                                                                                                                          >  7:549175/1‑151 (MQ=255)
                           tACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTAcc                                                                                                                         >  5:666181/1‑151 (MQ=255)
                               cACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGAtt                                                                                                                     <  5:324602/151‑1 (MQ=255)
                                aCTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGAttt                                                                                                                    >  2:706017/1‑151 (MQ=255)
                                 cTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTtc                                                                                                                   >  4:1584291/1‑151 (MQ=255)
                                   gCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTtctc                                                                                                                 >  1:1346951/1‑151 (MQ=255)
                                     cAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTtctc                                                                                                                 >  8:1303821/1‑149 (MQ=255)
                                     cAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAg                                                                                                               <  7:1671448/151‑1 (MQ=255)
                                     cAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAg                                                                                                               <  2:2735/151‑1 (MQ=255)
                                       gACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCa                                                                                                             <  7:73587/151‑1 (MQ=255)
                                       gACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCa                                                                                                             <  3:601312/151‑1 (MQ=255)
                                       gACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCa                                                                                                             <  2:778211/151‑1 (MQ=255)
                                         ccTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACg                                                                                                           <  3:778856/151‑1 (MQ=255)
                                           tCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGc                                                                                                          >  1:1478193/1‑150 (MQ=255)
                                            cAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCAt                                                                                                        <  8:224809/151‑1 (MQ=255)
                                             aGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCNCAGNANNCNtn                                                                                                       >  7:368207/1‑150 (MQ=255)
                                                 tGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGccc                                                                                                   >  6:277362/1‑151 (MQ=255)
                                                   gAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGc                                                                                                 <  2:1655409/151‑1 (MQ=255)
                                                    aGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCa                                                                                                <  1:465032/151‑1 (MQ=255)
                                                     gCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCaa                                                                                               <  7:1571297/151‑1 (MQ=255)
                                                        tgactgaACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAacac                                                                                            >  7:1225334/1‑151 (MQ=255)
                                                          actgaACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACAccc                                                                                          >  6:925889/1‑151 (MQ=255)
                                                            tgaACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACAccc                                                                                          >  6:461256/1‑149 (MQ=255)
                                                             gaACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAg                                                                                        <  6:85776/150‑1 (MQ=255)
                                                                    aTCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGatcatcat                                                                                >  4:910105/1‑151 (MQ=255)
                                                                    aTCCGTATAACAAAGTTATGCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTGACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGatcatcat                                                                                <  6:1540718/151‑1 (MQ=255)
                                                                     tCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGatcatcatc                                                                               >  1:807431/1‑151 (MQ=255)
                                                                       cGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATcc                                                                              >  2:1277215/1‑150 (MQ=255)
                                                                       cGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCa                                                                             <  5:52961/151‑1 (MQ=255)
                                                                       cGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCa                                                                             <  6:375830/151‑1 (MQ=255)
                                                                          ataACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAgg                                                                           >  2:617316/1‑150 (MQ=255)
                                                                            aacaacGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGgaa                                                                         <  2:1534605/150‑1 (MQ=255)
                                                                            aacaacGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGgaa                                                                         <  1:1084870/150‑1 (MQ=255)
                                                                            aacaacGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGga                                                                          >  1:1000300/1‑149 (MQ=255)
                                                                              caacGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGgaaga                                                                       >  5:1582901/1‑150 (MQ=255)
                                                                               aacGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAAt                                                                     <  6:143090/151‑1 (MQ=255)
                                                                               aacGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAAt                                                                     >  6:1296568/1‑151 (MQ=255)
                                                                                acGTTATCCGCACCACCATTGAAGCGGTGGCTGCTTCGCTGGGCGGTACTCAGTCACTGAATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATc                                                                    <  3:952773/151‑1 (MQ=255)
                                                                                acGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATc                                                                    <  3:1385191/151‑1 (MQ=255)
                                                                                 cGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATc                                                                    >  4:846386/1‑150 (MQ=255)
                                                                                  gTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATc                                                                    >  2:1015692/1‑149 (MQ=255)
                                                                                    tATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGa                                                                 >  8:794876/1‑150 (MQ=255)
                                                                                    tATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGa                                                                 >  8:1591836/1‑150 (MQ=255)
                                                                                    tATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGa                                                                 <  7:1151754/150‑1 (MQ=255)
                                                                                     aTCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAAc                                                               <  4:653699/151‑1 (MQ=255)
                                                                                       ccGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAActc                                                             >  7:687886/1‑151 (MQ=255)
                                                                                       ccGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAAct                                                              >  5:191288/1‑150 (MQ=255)
                                                                                         gCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTg                                                           >  8:1458163/1‑151 (MQ=255)
                                                                                         gCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTg                                                           >  1:1220903/1‑151 (MQ=255)
                                                                                          caccacCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGc                                                          >  2:1079652/1‑151 (MQ=255)
                                                                                           acctccATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGCCGAAGCGCTTGGTTTGCCTACCGATGTCTCAGCACGCATTGCGCGCCACACGCAGATCATCATCCAGGAAGAATCAGAGCTCTggc                                                         >  6:1704832/1‑149 (MQ=255)
                                                                                           accaccATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGcc                                                         >  2:847031/1‑151 (MQ=255)
                                                                                           accaccATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGcc                                                         <  4:751554/151‑1 (MQ=255)
                                                                                              accaTTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCa                                                      <  7:1491816/151‑1 (MQ=255)
                                                                                               ccaTTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCAc                                                     <  6:998205/151‑1 (MQ=255)
                                                                                                       gcgcTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATc                                             >  4:1335202/1‑151 (MQ=255)
                                                                                                         gctggctgCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATcc                                            >  4:694740/1‑150 (MQ=255)
                                                                                                         gctggctgCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCa                                           <  8:325475/151‑1 (MQ=255)
                                                                                                                gCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCg                                    <  3:843973/151‑1 (MQ=255)
                                                                                                                  gACGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCgg                                   >  3:1259359/1‑150 (MQ=255)
                                                                                                                   aCGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGAt                                 <  4:1461693/151‑1 (MQ=255)
                                                                                                                    cGCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATc                                <  1:867123/151‑1 (MQ=255)
                                                                                                                     gCTGGGCGGTACTCAGTCACTGCATAGCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATcc                               <  2:20254/151‑1 (MQ=255)
                                                                                                                     gCTGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATcc                               <  2:441333/151‑1 (MQ=255)
                                                                                                                       tGGGCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCt                              >  2:1366603/1‑150 (MQ=255)
                                                                                                                        gggCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCt                              <  8:1230341/149‑1 (MQ=255)
                                                                                                                        gggCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTAt                            <  6:718048/151‑1 (MQ=255)
                                                                                                                          gCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTAtt                           >  4:392450/1‑150 (MQ=255)
                                                                                                                          gCGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTAtt                           >  3:397350/1‑150 (MQ=255)
                                                                                                                           cGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTAc                         >  3:517644/1‑151 (MQ=255)
                                                                                                                           cGGTACTCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTAc                         >  7:1102815/1‑151 (MQ=255)
                                                                                                                            ggTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACa                        <  5:871046/151‑1 (MQ=255)
                                                                                                                                 tCAGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTg                     >  1:1542032/1‑149 (MQ=255)
                                                                                                                                   aGTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTc                 <  3:1638577/151‑1 (MQ=255)
                                                                                                                                    gTCACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCg                <  4:987741/151‑1 (MQ=255)
                                                                                                                                      cACTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCt              >  3:171872/1‑151 (MQ=255)
                                                                                                                                       aCTGCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTg             >  8:528091/1‑151 (MQ=255)
                                                                                                                                          gCATACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGAc           <  8:727075/150‑1 (MQ=255)
                                                                                                                                            aTACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGa        <  5:1426483/151‑1 (MQ=255)
                                                                                                                                            aTACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGa        <  1:391353/151‑1 (MQ=255)
                                                                                                                                            aTACCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTCCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGa        <  4:586046/151‑1 (MQ=255)
                                                                                                                                              aCCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGAt       <  7:1379205/150‑1 (MQ=255)
                                                                                                                                              aCCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATc      <  4:469753/151‑1 (MQ=255)
                                                                                                                                              aCCAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATc      <  3:1030264/151‑1 (MQ=255)
                                                                                                                                                cAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCa     >  8:696192/1‑150 (MQ=255)
                                                                                                                                                cAACGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCa     >  5:169705/1‑150 (MQ=255)
                                                                                                                                                 aaCGTCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCaa    >  5:796555/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
ACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAAT  >  NC_000913/3061767‑3062063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑