Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,089,260 T→C V326A (GTG→GCG)  galP → D‑galactose transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,089,2600TC97.5% 1292.2 / 8.4 356V326A (GTG→GCG) galPD‑galactose transporter
Reads supporting (aligned to +/- strand):  ref base T (5/4);  new base C (142/204);  total (148/208)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.61e-01

Only 100 of 357 total aligned reads displayed.

TCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCT  >  NC_000913/3089113‑3089406
                                                                                                                                                   |                                                                                                                                                  
tCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGAt                                                                                                                                                 >  5:367031/1‑151 (MQ=255)
   tccaaCTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGGCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATgg                                                                                                                                               <  6:144873/147‑1 (MQ=255)
   tCGAACTGGCGGGTTATACCAACACTACCGAGCAACTGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGtaa                                                                                                                                                  <  7:961036/147‑3 (MQ=255)
   tCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATg                                                                                                                                                <  8:379717/149‑1 (MQ=255)
   tCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGACCGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAATCCAACGCTAACTCTGGCATTCCTGGCTATgg                                                                                                                                               >  2:1454497/1‑150 (MQ=255)
    cGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGgct                                                                                                                                             <  4:1408266/151‑1 (MQ=255)
       aCTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGgctgct                                                                                                                                          <  1:1046763/151‑1 (MQ=255)
         tGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTgg                                                                                                                                        >  7:603470/1‑151 (MQ=255)
          ggCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGctgctg                                                                                                                                         <  7:695339/149‑1 (MQ=255)
          ggCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGctgctg                                                                                                                                         <  5:496071/149‑1 (MQ=255)
            cGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCAt                                                                                                                                     >  5:1135516/1‑151 (MQ=255)
            cGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCAt                                                                                                                                     <  2:94728/151‑1 (MQ=255)
             gggTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATg                                                                                                                                    <  3:1470685/151‑1 (MQ=255)
              ggTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATgg                                                                                                                                   <  1:88984/151‑1 (MQ=255)
               gTTATACCAACACTACCGAGGAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATggg                                                                                                                                  <  5:1566384/151‑1 (MQ=255)
                 tataCCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGc                                                                                                                                 <  8:855384/150‑1 (MQ=255)
                 tataCCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCg                                                                                                                                >  5:1537491/1‑151 (MQ=255)
                  ataCCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGt                                                                                                                               >  3:512450/1‑151 (MQ=255)
                     ccAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGCCCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACt                                                                                                                            >  5:671105/1‑151 (MQ=255)
                     ccAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCGTGGGCGTACt                                                                                                                            >  6:1357399/1‑151 (MQ=255)
                     ccAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACt                                                                                                                            <  8:1625200/151‑1 (MQ=255)
                     ccAACACTACCGAGCAAATGTGGGGGACCGTGATGGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACt                                                                                                                            <  1:305211/151‑1 (MQ=255)
                      cAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGGCTGACCAACGTACTTGCCACCTTTATCGCAATAGGCCTTCGTGACCGCTGCGGACGTAAACCAACGCAAACGCAGGGCTTCCTGGCGATGGCTGCTGGCATGGGCCTACTc                                                                                                                           >  4:1073004/1‑151 (MQ=255)
                       aaCACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCg                                                                                                                          >  6:492466/1‑151 (MQ=255)
                       aaCACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCg                                                                                                                          <  6:330559/151‑1 (MQ=255)
                         cacTACCGAGCAAATGT‑GGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGt                                                                                                                        >  1:1138276/1‑150 (MQ=255)
                           cTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTAc                                                                                                                      >  5:980423/1‑151 (MQ=255)
                                 aGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAatgat                                                                                                                >  1:198032/1‑151 (MQ=255)
                                    aaaTGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGc                                                                                                              >  4:986217/1‑150 (MQ=255)
                                      aTGTGGGGGCCCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGCTGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCaa                                                                                                            >  6:79350/1‑149 (MQ=255)
                                            gggACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTa                                                                                                     <  2:1258995/151‑1 (MQ=255)
                                            gggACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTa                                                                                                     <  3:172938/151‑1 (MQ=255)
                                              gcccGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTAtt                                                                                                   <  2:889863/149‑1 (MQ=255)
                                              gACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAatgatg                                                                                                               >  1:701887/1‑139 (MQ=255)
                                              gACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAatgatg                                                                                                               <  5:700235/139‑1 (MQ=255)
                                                  gTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCAct                                                                                               >  3:1325561/1‑151 (MQ=255)
                                                    gATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCActct                                                                                             <  8:132504/151‑1 (MQ=255)
                                                     aTTGTCGGCCTGACCAACGGAGTTGCCACGTTTCTCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTCGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACtctc                                                                                            <  4:555624/151‑1 (MQ=255)
                                                       tGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTcc                                                                                           <  8:492985/150‑1 (MQ=255)
                                                        gTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCg                                                                                          <  8:1223326/150‑1 (MQ=255)
                                                          cGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCg                                                                                       <  5:1631466/151‑1 (MQ=255)
                                                             ccTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGgcg                                                                                    >  3:937104/1‑151 (MQ=255)
                                                               ggACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCGGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCa                                                                                  <  5:905179/150‑1 (MQ=255)
                                                                gACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCa                                                                                  <  4:435732/150‑1 (MQ=255)
                                                                gACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCa                                                                                  <  5:722220/150‑1 (MQ=255)
                                                                 aCCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCCCTCTCCGTCGGCGCAGt                                                                                >  1:1182658/1‑151 (MQ=255)
                                                                  ccAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGt                                                                                >  7:1179439/1‑150 (MQ=255)
                                                                    aaCGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTAtt                                                                             <  1:367994/151‑1 (MQ=255)
                                                                      cGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTtc                                                                           >  7:760491/1‑151 (MQ=255)
                                                                      cGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTtc                                                                           <  2:1407338/151‑1 (MQ=255)
                                                                       gTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTtcg                                                                          >  5:1205107/1‑151 (MQ=255)
                                                                       gTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTtcg                                                                          <  3:637005/151‑1 (MQ=255)
                                                                         aCTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCGGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTtcgc                                                                         <  4:1608481/150‑1 (MQ=255)
                                                                            tGCGACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTtggccat                                                                      <  2:1171013/150‑1 (MQ=255)
                                                                            tGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTtcgcca                                                                       <  1:1415742/149‑1 (MQ=255)
                                                                                   tttATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCAtgc                                                              >  3:990967/1‑151 (MQ=255)
                                                                                    ttATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCAtgct                                                             >  5:415028/1‑151 (MQ=255)
                                                                                     tATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCAtgctg                                                            >  2:744486/1‑151 (MQ=255)
                                                                                      atcgctttgggCGTTGTTGACCGCTGGGGACGTAATGGATAGCTAATTCTGTGCTTCGTGGCGATGGCTGCTTGAATGGTCGTACTAGGTACAATGATGGATATCGGTATTCACTCTACGTCGGCGCAGTATTTggcgatcgcgattgtg                                                            <  4:1259991/141‑5 (MQ=255)
                                                                                      aTCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCAtgctgc                                                           >  3:589261/1‑151 (MQ=255)
                                                                                        cGCAATCGGCCTTGTTGACCGCTGGGGACGTACACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGctgctg                                                         <  6:1083818/151‑1 (MQ=255)
                                                                                        cGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATgctgct                                                          <  2:1582594/150‑1 (MQ=255)
                                                                                        cGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATgctgct                                                          <  3:75390/150‑1 (MQ=255)
                                                                                        cGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGctgctg                                                         >  2:1528310/1‑151 (MQ=255)
                                                                                         gCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGctgctg                                                         >  4:481982/1‑150 (MQ=255)
                                                                                          cAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGAt                                                       <  6:603872/151‑1 (MQ=255)
                                                                                           aaTCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATg                                                      <  2:477079/151‑1 (MQ=255)
                                                                                            aTCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGt                                                     >  1:1619084/1‑151 (MQ=255)
                                                                                            aTCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGt                                                     >  5:1647515/1‑151 (MQ=255)
                                                                                              cGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGttt                                                   <  3:1096733/151‑1 (MQ=255)
                                                                                                   ttgttgACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGt                                              >  3:1427274/1‑151 (MQ=255)
                                                                                                   ttgttgACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGt                                              >  8:1483265/1‑151 (MQ=255)
                                                                                                    tgttgAGCGCTGGGGACGTAAACCAACGCTAACGCTGTGCTTCCTGGCGATGGCTGCTGGCATGGGCGTAGTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGt                                              <  4:808564/150‑1 (MQ=255)
                                                                                                    tgttgACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTg                                               <  8:462456/149‑1 (MQ=255)
                                                                                                     gttgACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTc                                             <  2:315927/150‑1 (MQ=255)
                                                                                                      ttgACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCgg                                           >  3:1303329/1‑151 (MQ=255)
                                                                                                        gACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGtt                                         <  7:217214/151‑1 (MQ=255)
                                                                                                          ccGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGtttt                                       <  4:1207565/151‑1 (MQ=255)
                                                                                                           cGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTg                                      <  5:528936/151‑1 (MQ=255)
                                                                                                           cGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTg                                      <  4:1657441/151‑1 (MQ=255)
                                                                                                              tGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGcc                                    >  5:1333559/1‑150 (MQ=255)
                                                                                                                 ggACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATg                                 >  5:288666/1‑150 (MQ=255)
                                                                                                                     gTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGc                            >  5:853002/1‑151 (MQ=255)
                                                                                                                       aaaCCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCg                          <  7:1383328/151‑1 (MQ=255)
                                                                                                                       aaaCCAACGCTAACGCTGGGCTTCCTGGCGATGGCGGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCTGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCg                          <  3:1205905/151‑1 (MQ=255)
                                                                                                                       aaaCCAACGCTAACGCTGGGCTTCCTCGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGACATGCTGCTGATGTTTATTGACGGTTTTGCCATGAGTGCCg                          >  1:1359233/1‑151 (MQ=255)
                                                                                                                           cAACGCTAACGCTGGGCTGCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTcc                      <  3:561415/151‑1 (MQ=255)
                                                                                                                            aacgctaagggtGGGCTTCCTGGCGATGGCTGCTGGGATGGGCGTACTCGGTTGAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGGGATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTcgg                     <  7:1107038/151‑3 (MQ=255)
                                                                                                                            aacgctaacgctGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCg                     >  7:431261/1‑151 (MQ=255)
                                                                                                                                 taacgctGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGAt                >  1:1240013/1‑151 (MQ=255)
                                                                                                                                  aacgctGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGAtt               <  3:114784/151‑1 (MQ=255)
                                                                                                                                  aacgctGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGAtt               <  3:877076/151‑1 (MQ=255)
                                                                                                                                    cgctGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTg             >  6:706538/1‑151 (MQ=255)
                                                                                                                                    cgctGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTg             <  2:779288/151‑1 (MQ=255)
                                                                                                                                     gctGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTgg            >  2:1068882/1‑151 (MQ=255)
                                                                                                                                          gCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGGTGATTTGGGTACt       >  7:1560483/1‑151 (MQ=255)
                                                                                                                                            ttCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACtgt     >  3:1266930/1‑151 (MQ=255)
                                                                                                                                            ttCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACtgt     >  1:8261/1‑151 (MQ=255)
                                                                                                                                            ttCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACtgt     >  8:76513/1‑151 (MQ=255)
                                                                                                                                                tGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTg             <  2:614809/139‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCT  >  NC_000913/3089113‑3089406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑