Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,120,786 G→A T165I (ACT→ATT)  glcA ← glycolate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,120,7860GA96.7% 1344.3 / 19.8 400T165I (ACT→ATT) glcAglycolate transporter
Reads supporting (aligned to +/- strand):  ref base G (9/4);  new base A (189/197);  total (199/201)
Fisher's exact test for biased strand distribution p-value = 1.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 402 total aligned reads displayed.

CATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGC  >  NC_000913/3120638‑3120932
                                                                                                                                                    |                                                                                                                                                  
   catTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATAtt                                                                                                                                                <  8:1200302/150‑1 (MQ=255)
   catTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATAtt                                                                                                                                                <  4:425774/150‑1 (MQ=255)
   catTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATAtt                                                                                                                                                <  3:965087/150‑1 (MQ=255)
    atTGCTACCAGACAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTCGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAAAGCCACCGGCGCAATAttcg                                                                                                                                              >  5:478994/1‑149 (MQ=255)
            cagccagAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGa                                                                                                                                      <  6:76269/151‑1 (MQ=255)
                cagAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGAcaca                                                                                                                                  <  7:395562/151‑1 (MQ=255)
                 agAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGacac                                                                                                                                   >  5:1119551/1‑149 (MQ=255)
                 agAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTAGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGacac                                                                                                                                   >  5:128828/1‑149 (MQ=255)
                  gAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAATGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGc                                                                                                                                >  2:440500/1‑151 (MQ=255)
                  gAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGc                                                                                                                                >  8:137128/1‑151 (MQ=255)
                  gAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGc                                                                                                                                <  8:83333/151‑1 (MQ=255)
                   aaCGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGcc                                                                                                                               >  3:575425/1‑151 (MQ=255)
                    aCGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGccc                                                                                                                              >  3:837551/1‑151 (MQ=255)
                    aCGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGccc                                                                                                                              >  6:304979/1‑151 (MQ=255)
                     cGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGcccc                                                                                                                             >  3:1254576/1‑151 (MQ=255)
                       gCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCccgc                                                                                                                           <  1:1178049/151‑1 (MQ=255)
                        cacaAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCAGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCccgcc                                                                                                                          <  6:1696271/151‑1 (MQ=255)
                            aGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGt                                                                                                                      >  8:452807/1‑151 (MQ=255)
                              aaCCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGtat                                                                                                                    <  1:495415/151‑1 (MQ=255)
                               aCCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGtata                                                                                                                   <  7:85298/151‑1 (MQ=255)
                                  gACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACg                                                                                                                >  5:746706/1‑151 (MQ=255)
                                     aGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGtt                                                                                                             >  7:1546365/1‑151 (MQ=255)
                                        aaCGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTg                                                                                                           >  5:308211/1‑150 (MQ=255)
                                        aaCGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTg                                                                                                           >  5:248905/1‑150 (MQ=255)
                                        aaCGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGa                                                                                                          <  1:1601408/151‑1 (MQ=255)
                                         aCGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGaa                                                                                                         <  4:1100920/151‑1 (MQ=255)
                                         aCGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGa                                                                                                          >  4:876702/1‑150 (MQ=255)
                                         aCGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGa                                                                                                          >  8:1582877/1‑150 (MQ=255)
                                          cGGTAACTGACGTCCCGCCATTGCGCCAATGCGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAACCGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAg                                                                                                        <  4:219142/151‑1 (MQ=255)
                                                     gTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCccac                                                                                             >  8:1478173/1‑151 (MQ=255)
                                                       cccGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGACGCGTATAACGGTTTGAAGCCCAGGCccacc                                                                                            >  6:1183758/1‑150 (MQ=255)
                                                        ccGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACcag                                                                                          <  6:1585702/151‑1 (MQ=255)
                                                         cGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACcagc                                                                                         <  4:1514149/151‑1 (MQ=255)
                                                         cGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACcagc                                                                                         <  6:604671/151‑1 (MQ=255)
                                                          gCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACcagca                                                                                        <  4:137091/151‑1 (MQ=255)
                                                            cATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCagcagc                                                                                      <  6:1009822/151‑1 (MQ=255)
                                                              ttGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAgcgc                                                                                    >  2:1248244/1‑151 (MQ=255)
                                                                  gcCAATGTGGACCGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCAGCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGGATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCg                                                                                <  2:1157857/151‑1 (MQ=255)
                                                                    cAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCACACCAGCAGCGCACCGGt                                                                              >  4:927783/1‑151 (MQ=255)
                                                                     aaTGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACAAGCAGCGCACCGGt                                                                              >  3:705799/1‑150 (MQ=255)
                                                                        gtgGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCGACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATc                                                                          >  6:912260/1‑151 (MQ=255)
                                                                        gtgGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATc                                                                          <  3:14567/151‑1 (MQ=255)
                                                                        gtgGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATc                                                                          <  2:1336989/151‑1 (MQ=255)
                                                                        gtgGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATc                                                                          <  1:1467338/151‑1 (MQ=255)
                                                                            aaCGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGcc                                                                       >  3:456033/1‑150 (MQ=255)
                                                                            aaCGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCa                                                                      <  5:1622633/151‑1 (MQ=255)
                                                                                 aTCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGgc                                                                 >  8:335303/1‑151 (MQ=255)
                                                                                    gATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGgcgc                                                               >  7:988356/1‑150 (MQ=255)
                                                                                     aTTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCAc                                                             >  6:1469597/1‑151 (MQ=255)
                                                                                       tCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAATGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCAcc                                                            >  2:863515/1‑150 (MQ=255)
                                                                                         ccGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCaaa                                                         <  1:187323/151‑1 (MQ=255)
                                                                                         ccGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCaaa                                                         <  4:1280181/151‑1 (MQ=255)
                                                                                          cGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGGTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAag                                                        <  4:313994/151‑1 (MQ=255)
                                                                                           gTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAagc                                                       >  6:691918/1‑151 (MQ=255)
                                                                                             tACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGAAATCAGACACAGCCCAGCCGCGTATAACGGATTGAAGCACAGGCCAACAACCATCTCAACGGTAATCGCCACAGCCCCACAAAagcc                                                      >  6:1360367/1‑150 (MQ=255)
                                                                                              aCCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAagccag                                                    <  3:1595525/151‑1 (MQ=255)
                                                                                              aCCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAagcc                                                      >  3:312838/1‑149 (MQ=255)
                                                                                              aCCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAagcc                                                      >  3:1194296/1‑149 (MQ=255)
                                                                                              aCCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAagcc                                                      >  5:937018/1‑149 (MQ=255)
                                                                                              aCCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAagcc                                                      >  7:171231/1‑149 (MQ=255)
                                                                                                  gACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCCGCAGCGCACCGGTAATCGCCACCGGCGCACCAACGCCAGCCGc                                                >  6:395452/1‑151 (MQ=255)
                                                                                                   aCCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCt                                               >  2:1456673/1‑151 (MQ=255)
                                                                                                     cGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTcc                                             <  4:1158020/151‑1 (MQ=255)
                                                                                                      ggCGACCTGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCGCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCt                                            <  4:6600/151‑1 (MQ=255)
                                                                                                      ggCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCt                                            >  6:503461/1‑151 (MQ=255)
                                                                                                          aCCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCa                                        <  1:672863/151‑1 (MQ=255)
                                                                                                           ccAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGCCACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCAACAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTcc                                         >  1:1672005/1‑149 (MQ=255)
                                                                                                           ccAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTcc                                         >  5:608473/1‑149 (MQ=255)
                                                                                                            cAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACCCAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTGCCAGc                                      >  7:664814/1‑151 (MQ=255)
                                                                                                            cAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGc                                      <  2:83372/151‑1 (MQ=255)
                                                                                                             aGAATCGGCACGCCCCACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCGCCGCGGATAACGGTTTGAAGCCCCGGCCCACCCGCAGCGCCCCGGTCATCGCCACCGGCGCACCCAAGCCAGCCGCGCCTTCCAGcc                                     >  1:431092/1‑150 (MQ=255)
                                                                                                              gAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCaa                                    <  5:1063349/151‑1 (MQ=255)
                                                                                                              caagcGGCACGCCCAACGCACCAAACGCCAACGGCGCAATATTGGCAATCAGACCCAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGTCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCaa                                    <  3:23271/147‑1 (MQ=255)
                                                                                                               aaTCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCGCCGCCTCGTATAACGGTTTGAAGCCCAGGCGCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAAc                                   <  7:924927/151‑1 (MQ=255)
                                                                                                               aaTCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAAc                                   <  2:603591/151‑1 (MQ=255)
                                                                                                               aaTCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAAc                                   >  2:712411/1‑151 (MQ=255)
                                                                                                                     cACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCAcc                              >  7:1287350/1‑150 (MQ=255)
                                                                                                                     cACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCAcc                              >  7:1030276/1‑150 (MQ=255)
                                                                                                                     cACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCa                             <  5:1432353/151‑1 (MQ=255)
                                                                                                                       cGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCaaa                           <  8:1053062/151‑1 (MQ=255)
                                                                                                                          ccAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAgg                         >  7:20761/1‑150 (MQ=255)
                                                                                                                          ccAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGga                        <  1:849333/151‑1 (MQ=255)
                                                                                                                           cAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCCCCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAgg                         >  8:492859/1‑149 (MQ=255)
                                                                                                                           cAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAgg                         >  1:1390545/1‑149 (MQ=255)
                                                                                                                           cAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCCCCGCGTATAACGGTTTGAAGCCCAGGCCCAACAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCTCTCCTTCCAGCAACGCACCAAACgac                       >  4:747138/1‑150 (MQ=255)
                                                                                                                           aaaCGCACCAAACTCCACGCGCGCAAAATTGCCAAACAAACACACCCCCGCCGCGTATAACGGTGTGAAGCCCACGCCCACCAGCAGCGCAACGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACTCACCAAAGgag                       <  6:199578/150‑1 (MQ=255)
                                                                                                                            aaCGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAgg                         >  3:91462/1‑148 (MQ=255)
                                                                                                                            aaCGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGgaga                      <  8:735832/151‑1 (MQ=255)
                                                                                                                              cGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGaa                     >  1:129925/1‑150 (MQ=255)
                                                                                                                                cACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAAcc                  <  7:477113/151‑1 (MQ=255)
                                                                                                                                   cAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGGTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAAt               <  2:1633251/151‑1 (MQ=255)
                                                                                                                                        gCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGt           >  1:1093502/1‑150 (MQ=255)
                                                                                                                                         ccACCGGCGCAATATTGGCAATCAGCCACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGCAACCAATCAGTa          >  3:1022554/1‑150 (MQ=255)
                                                                                                                                           aCCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAacc       >  2:109252/1‑150 (MQ=255)
                                                                                                                                           aCCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAacc       >  2:785505/1‑150 (MQ=255)
                                                                                                                                           aCCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAacc       >  3:109857/1‑150 (MQ=255)
                                                                                                                                           aCCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAaca       <  1:930364/151‑1 (MQ=255)
                                                                                                                                              ggCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCt    <  1:284132/151‑1 (MQ=255)
                                                                                                                                                cgcAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGc  <  2:304500/151‑1 (MQ=255)
                                                                                                                                                 gcAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACTGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGc  >  4:1446549/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGC  >  NC_000913/3120638‑3120932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑