BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,162,216	G→A	Q152* (CAG→TAG)	ftsP ←	septal ring component that protects the divisome from stress; multicopy suppressor of ftsI(Ts)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,162,216	0	G	A	97.3%	1405.6 / 14.9	412	Q152* (CAG→TAG)	ftsP	septal ring component that protects the divisome from stress; multicopy suppressor of ftsI(Ts)
Reads supporting (aligned to +/- strand): ref base G (9/2); new base A (231/170); total (240/172)
Fisher's exact test for biased strand distribution p-value = 1.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 423 total aligned reads displayed.

TCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCAC  >  NC_000913/3162068‑3162363

 cGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACctactg                                                                                                                                                  >  8:1677399/1‑151 (MQ=255)

  gTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGa                                                                                                                                                 <  1:1465627/151‑1 (MQ=255)

    tGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCAACATTCAGGCAATGCCGTTATAGACCTACTGAGc                                                                                                                                               >  3:217400/1‑151 (MQ=255)

         tCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTgg                                                                                                                                          >  4:228338/1‑150 (MQ=255)

          cttgCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCg                                                                                                                                         >  5:1247314/4‑151 (MQ=255)

            ggCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGc                                                                                                                                          <  7:160858/148‑1 (MQ=255)

             gCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGtt                                                                                                                                      >  7:531438/1‑151 (MQ=255)

              cGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTg                                                                                                                                     <  6:1278932/151‑1 (MQ=255)

              cGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTg                                                                                                                                     <  8:1090723/151‑1 (MQ=255)

                 acctacgTTATCGAGCCGTTTCTCCTGGATAATGACCGGAACAGGATGCACACCATAATGGTTGGGGATAGGCTGCGAGTTGCTGACTTCATCTTCCACAAGCCACATTCCGGCAAGGCAGTTTTAGACCTACTGAGCCGTGCGGTTGGGa                                                                                                                                  <  8:906800/145‑1 (MQ=255)

                   cAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTCCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGt                                                                                                                                >  4:1232637/1‑151 (MQ=255)

                    aaaGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGt                                                                                                                                >  7:1214538/1‑150 (MQ=255)

                    aaaGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTa                                                                                                                               <  2:835506/151‑1 (MQ=255)

                     aaGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTAt                                                                                                                              >  1:935909/1‑151 (MQ=255)

                       gTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTg                                                                                                                            <  3:1471294/151‑1 (MQ=255)

                          aTCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCg                                                                                                                         >  8:1172580/1‑151 (MQ=255)

                           tCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGt                                                                                                                        >  4:593423/1‑151 (MQ=255)

                            ccAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGt                                                                                                                        <  4:879034/150‑1 (MQ=255)

                             cAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTg                                                                                                                       >  2:318495/1‑150 (MQ=255)

                             cAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTg                                                                                                                       >  2:404713/1‑150 (MQ=255)

                                 cGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATAcc                                                                                                                  >  5:1599473/1‑151 (MQ=255)

                                  gTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATAcc                                                                                                                  >  8:1250845/1‑150 (MQ=255)

                                  gTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACca                                                                                                                 <  3:1415686/151‑1 (MQ=255)

                                    ttATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACcac                                                                                                                >  8:312251/1‑150 (MQ=255)

                                     tATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACcac                                                                                                                >  7:684921/1‑149 (MQ=255)

                                       tCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACag                                                                                                              >  7:307756/1‑149 (MQ=255)

                                       tCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACag                                                                                                              >  3:310375/1‑149 (MQ=255)

                                       tCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACag                                                                                                              >  6:1047481/1‑149 (MQ=255)

                                         cTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGt                                                                                                           >  7:448229/1‑150 (MQ=255)

                                          tGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGt                                                                                                           >  7:1136073/1‑149 (MQ=255)

                                           ggATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTa                                                                                                          <  8:1618681/149‑1 (MQ=255)

                                           ggATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAg                                                                                                         <  3:869938/150‑1 (MQ=255)

                                           ggATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCGGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAg                                                                                                         <  5:351235/150‑1 (MQ=255)

                                               aaTGACCGGAAAATCATCCACACCATAATTGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGc                                                                                                     <  8:1560284/150‑1 (MQ=255)

                                                aTGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGt                                                                                                   >  3:1298756/1‑151 (MQ=255)

                                                  gACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTc                                                                                                 >  3:1168302/1‑151 (MQ=255)

                                                      ggAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGc                                                                                             <  6:473224/151‑1 (MQ=255)

                                                      ggAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGc                                                                                             <  8:765922/151‑1 (MQ=255)

                                                        aaaaTCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTCGCTGCGTTCTGGCg                                                                                            >  6:15768/1‑150 (MQ=255)

                                                        aaaaTCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCg                                                                                            >  8:297792/1‑150 (MQ=255)

                                                        aaaaTCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCATTCTGGCg                                                                                            >  6:1484975/1‑150 (MQ=255)

                                                         aaaTCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGaa                                                                                          <  4:1594443/151‑1 (MQ=255)

                                                         aaaTCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGa                                                                                           >  6:893550/1‑150 (MQ=255)

                                                           atcatcCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATg                                                                                        >  7:728552/1‑151 (MQ=255)

                                                           atcatcCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATg                                                                                        >  3:1561840/1‑151 (MQ=255)

                                                             catcCACTCGATTATGGTTGGGGATAGGCAGCGACTTGCTGAGTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTTAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATggg                                                                                      <  5:173739/151‑1 (MQ=255)

                                                             catcCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATggg                                                                                      <  1:747738/151‑1 (MQ=255)

                                                                 cacaCCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGt                                                                                  >  2:1333299/1‑151 (MQ=255)

                                                                    acCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACg                                                                               <  4:468674/151‑1 (MQ=255)

                                                                     ccATAATGGTTGGGGATAGGCAGCGACTTGCTGCCTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACgg                                                                              >  5:1065284/1‑151 (MQ=255)

                                                                      cATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACATACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACggg                                                                             >  8:857626/1‑151 (MQ=255)

                                                                       aTAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGt                                                                            >  6:360896/1‑151 (MQ=255)

                                                                       aTAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGt                                                                            >  5:269806/1‑151 (MQ=255)

                                                                       aTAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGt                                                                            >  8:729753/1‑151 (MQ=255)

                                                                           tGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGccc                                                                        >  2:921208/1‑151 (MQ=255)

                                                                            ggTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCa                                                                       <  8:1388642/151‑1 (MQ=255)

                                                                             gTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGccc                                                                        >  8:1065373/1‑149 (MQ=255)

                                                                             gTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAg                                                                      <  7:874573/151‑1 (MQ=255)

                                                                               tGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTc                                                                    >  6:1671286/1‑151 (MQ=255)

                                                                                ggtgATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGTGCAGTACGGGTGCCCAGTCa                                                                   <  2:219332/148‑1 (MQ=255)

                                                                                 gggATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGGGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAg                                                                  <  5:1161631/151‑1 (MQ=255)

                                                                                      aGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGttt                                                             <  1:1592912/151‑1 (MQ=255)

                                                                                      aGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGtt                                                              <  6:611033/150‑1 (MQ=255)

                                                                                         cAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGc                                                          >  4:1464641/1‑151 (MQ=255)

                                                                                         cAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGc                                                          >  1:365192/1‑151 (MQ=255)

                                                                                          aGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCg                                                         >  6:772884/1‑151 (MQ=255)

                                                                                          aGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCg                                                         >  8:145733/1‑151 (MQ=255)

                                                                                            cGACTTCCTGACTTCATCTTCCACCAGCCACATTCCGGCAACGCCGTTATAGACCTACTGATCCGTGCGGTTGGGAGTATTGGCGTCATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGa                                                        >  1:1041977/1‑150 (MQ=255)

                                                                                             gACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGAc                                                       >  5:413238/1‑150 (MQ=255)

                                                                                             gACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCAGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGAc                                                       >  3:474976/1‑150 (MQ=255)

                                                                                                  gCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGAcatcat                                                  <  3:1337553/150‑1 (MQ=255)

                                                                                                      aCTTCATCTTCCACCAGCCACATTCCGGGAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTg                                             <  6:1126369/151‑1 (MQ=255)

                                                                                                       cTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGc                                            >  1:654168/1‑151 (MQ=255)

                                                                                                       cTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTCGCGAATGCGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGc                                            >  4:1048678/1‑151 (MQ=255)

                                                                                                        ttCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGCCATCATCCGTGcc                                           >  4:140708/1‑151 (MQ=255)

                                                                                                          cATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCgg                                         >  8:487430/1‑151 (MQ=255)

                                                                                                          cATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCgg                                         >  3:527792/1‑151 (MQ=255)

                                                                                                                   ccaGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCa                                 >  6:1602560/1‑150 (MQ=255)

                                                                                                                   ccaGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCAt                                <  1:1173060/151‑1 (MQ=255)

                                                                                                                   ccaGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCAt                                <  1:1258849/151‑1 (MQ=255)

                                                                                                                   ccaGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCAt                                >  2:105970/1‑151 (MQ=255)

                                                                                                                    caGCCACATTCCGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATc                               >  3:589172/1‑151 (MQ=255)

                                                                                                                          caatCCGGCAAGGCGTTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCTTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCgtg                         <  6:455009/148‑3 (MQ=255)

                                                                                                                              ccGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCCTCAGCGGGCCTg                     >  1:819884/1‑151 (MQ=255)

                                                                                                                               cGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTgg                    >  7:1038334/1‑151 (MQ=255)

                                                                                                                               cGGCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTgg                    >  2:1435586/1‑151 (MQ=255)

                                                                                                                                 gCAAGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTa                  <  7:922989/151‑1 (MQ=255)

                                                                                                                                   aaGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTAcc                >  5:762980/1‑151 (MQ=255)

                                                                                                                                    aGGCCGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCt               >  4:1356048/1‑151 (MQ=255)

                                                                                                                                       ccGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGt             >  5:1405232/1‑150 (MQ=255)

                                                                                                                                       ccGTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTa            <  2:158331/151‑1 (MQ=255)

                                                                                                                                         gTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGc          <  2:567240/151‑1 (MQ=255)

                                                                                                                                         gTTATAGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGc          <  4:999067/151‑1 (MQ=255)

                                                                                                                                           tataGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGGAGccc        >  4:1165880/1‑151 (MQ=255)

                                                                                                                                           tataGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGGGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGcc         <  3:1015161/150‑1 (MQ=255)

                                                                                                                                            ataGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGccc        <  2:679807/150‑1 (MQ=255)

                                                                                                                                              aGACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGCGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCgg      <  7:138094/150‑1 (MQ=255)

                                                                                                                                               gACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGcc    >  8:422312/1‑151 (MQ=255)

                                                                                                                                               gACCTACTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGCGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCGCGGcc    >  2:1179874/1‑151 (MQ=255)

                                                                                                                                               gACCTACTGAGCCGTGCGGGTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGcc    <  5:1657297/151‑1 (MQ=255)

TCGTTGTATTCTGGCGTACCAAAGTTATCCAGCCGTTTATCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCAC  >  NC_000913/3162068‑3162363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`