Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 281,291 G→A I231I (ATC→ATT)  yagA ← CP4‑6 prophage; putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913281,2910GA95.1% 1578.4 / 47.6 488I231I (ATC→ATT) yagACP4‑6 prophage; putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (13/11);  new base A (269/195);  total (282/206)
Fisher's exact test for biased strand distribution p-value = 8.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 488 total aligned reads displayed.

AGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGGATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCG  >  NC_000913/281143‑281437
                                                                                                                                                    |                                                                                                                                                  
 gACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAAt                                                                                                                                                  >  2:227162/1‑150 (MQ=255)
 gACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAAt                                                                                                                                                  >  1:198695/1‑150 (MQ=255)
   ccGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCA‑TTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATAacc                                                                                                                                              >  4:1259238/1‑151 (MQ=255)
       ccGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAgg                                                                                                                                           <  8:83647/151‑1 (MQ=255)
       ccGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAgg                                                                                                                                           <  7:212673/151‑1 (MQ=255)
         gCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGgcc                                                                                                                                         >  1:1330206/1‑150 (MQ=255)
          ccAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGgcgc                                                                                                                                        <  2:803615/151‑1 (MQ=255)
             gTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATc                                                                                                                                     >  4:1432435/1‑151 (MQ=255)
                 tCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGcc                                                                                                                                 >  5:1324810/1‑151 (MQ=255)
                 tCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGcc                                                                                                                                 >  8:177292/1‑151 (MQ=255)
                  cGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCca                                                                                                                                <  1:65174/151‑1 (MQ=255)
                     aGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACACAGGCGCATCAGCcac                                                                                                                               >  6:1361061/1‑149 (MQ=255)
                       gcgcgcTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCcac                                                                                                                               >  6:488458/1‑147 (MQ=255)
                         gcgcTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTcc                                                                                                                         >  4:947941/1‑151 (MQ=255)
                          cgcTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCa                                                                                                                        <  5:192513/151‑1 (MQ=255)
                           gcTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCac                                                                                                                       >  4:306734/1‑150 (MQ=255)
                           gcTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAg                                                                                                                       <  4:1571144/151‑1 (MQ=255)
                             tGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGTGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAgcc                                                                                                                     >  4:465981/1‑150 (MQ=255)
                             tGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAgcg                                                                                                                     >  8:1554332/1‑151 (MQ=255)
                                aGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGGATACCCAGGCGCATCAGCCACAGCTCCAGCGccc                                                                                                                  >  3:733764/1‑150 (MQ=255)
                                    cACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTcc                                                                                                               >  4:1311629/1‑150 (MQ=255)
                                      ccGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAgg                                                                                                            <  8:409896/151‑1 (MQ=255)
                                         cTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGc                                                                                                         >  6:95959/1‑151 (MQ=255)
                                         cTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGc                                                                                                         >  5:626197/1‑151 (MQ=255)
                                          tGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGGATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGcc                                                                                                        >  1:1045079/1‑151 (MQ=255)
                                           gTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCGGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCg                                                                                                       <  2:189591/151‑1 (MQ=255)
                                            tCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCgg                                                                                                      >  2:1472519/1‑151 (MQ=255)
                                            tCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCgg                                                                                                      >  1:949867/1‑151 (MQ=255)
                                             cTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCggt                                                                                                     >  6:1043293/1‑151 (MQ=255)
                                                 gAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGgtgctg                                                                                                 >  2:911022/1‑151 (MQ=255)
                                                  aaCCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGgtgt                                                                                                <  2:729862/151‑1 (MQ=255)
                                                  aaCCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGgtgt                                                                                                >  1:783074/1‑151 (MQ=255)
                                                   aCCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTc                                                                                               >  7:986177/1‑151 (MQ=255)
                                                   aCCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTc                                                                                               <  7:839634/151‑1 (MQ=255)
                                                    ccATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCg                                                                                              >  2:269188/1‑151 (MQ=255)
                                                      aTTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGcc                                                                                            >  6:1303445/1‑151 (MQ=255)
                                                         ttCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGcccc                                                                                          >  2:1373940/1‑150 (MQ=255)
                                                           cccTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCAc                                                                                        <  3:1635933/150‑1 (MQ=255)
                                                              tGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTg                                                                                    >  6:1160906/1‑151 (MQ=255)
                                                               gcagcaCTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTg                                                                                    <  5:269032/150‑1 (MQ=255)
                                                               gcagcaCTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTg                                                                                    >  8:1625700/1‑150 (MQ=255)
                                                                cagcaCTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTg                                                                                    >  6:826702/1‑149 (MQ=255)
                                                                   caCTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCg                                                                               >  3:1266766/1‑151 (MQ=255)
                                                                      ttCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGtt                                                                             >  3:57116/1‑150 (MQ=255)
                                                                       tCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTAt                                                                           >  4:815239/1‑151 (MQ=255)
                                                                          gCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATcc                                                                         >  6:898447/1‑150 (MQ=255)
                                                                           ccTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCAt                                                                       <  6:1551095/151‑1 (MQ=255)
                                                                           ccTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCAt                                                                       >  3:424810/1‑151 (MQ=255)
                                                                             ttCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATgg                                                                     >  3:821449/1‑151 (MQ=255)
                                                                              tCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGt                                                                    >  1:1256087/1‑151 (MQ=255)
                                                                              tCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGGCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATg                                                                      <  2:571284/149‑1 (MQ=255)
                                                                               cAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTc                                                                   >  6:24415/1‑151 (MQ=255)
                                                                                aGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCTGTTATCCATGGTCa                                                                  <  6:345092/151‑1 (MQ=255)
                                                                                aGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTc                                                                   >  6:171350/1‑150 (MQ=255)
                                                                                aGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTc                                                                   >  8:658042/1‑150 (MQ=255)
                                                                                 ggCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCAt                                                                 >  2:1068586/1‑151 (MQ=255)
                                                                                  gCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCAtc                                                                <  1:28648/151‑1 (MQ=255)
                                                                                   cTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCAtcc                                                               >  1:1614442/1‑151 (MQ=255)
                                                                                    ggCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCAtccg                                                              <  8:51196/150‑1 (MQ=255)
                                                                                     gCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCAtccgg                                                             <  8:1322192/151‑1 (MQ=255)
                                                                                        gTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCAtccggtcc                                                          <  7:1613157/151‑1 (MQ=255)
                                                                                          gAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATACGGCCGGGAGTGCCCCACCCGAATACCCCGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCAtccggtccg                                                         <  4:67748/150‑1 (MQ=255)
                                                                                          gAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCTAATACCCAGTCGCATCAGCCACAGCTCCAGCGCCGTCCAGGAGCCGGTGGTCTCGCCCCACGGTGAGCCGTTATCCATGCTCAtccggtccgg                                                        >  6:1217737/1‑151 (MQ=255)
                                                                                             aaCGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAg                                                     <  7:640012/151‑1 (MQ=255)
                                                                                               cGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGc                                                   >  7:1496704/1‑151 (MQ=255)
                                                                                                  tCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGt                                                >  4:242708/1‑151 (MQ=255)
                                                                                                   ccAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGt                                                >  4:573300/1‑150 (MQ=255)
                                                                                                    cAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTaa                                              <  5:1412511/151‑1 (MQ=255)
                                                                                                    cAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTa                                               >  7:369494/1‑150 (MQ=255)
                                                                                                    cAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGGGCCCCACCCGAATACCCAGCCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTaa                                              <  5:1040523/151‑1 (MQ=255)
                                                                                                     aGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAAc                                             >  8:1490771/1‑151 (MQ=255)
                                                                                                     aGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAAc                                             >  6:1635149/1‑151 (MQ=255)
                                                                                                      gCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACg                                            <  3:1428887/151‑1 (MQ=255)
                                                                                                              tGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAac                                     >  8:139515/1‑150 (MQ=255)
                                                                                                              tGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAac                                     >  3:906550/1‑150 (MQ=255)
                                                                                                               gCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATGAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAaca                                    <  5:1562657/150‑1 (MQ=255)
                                                                                                               gCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAaca                                    <  3:1556612/150‑1 (MQ=255)
                                                                                                               gCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAaca                                    <  1:40543/150‑1 (MQ=255)
                                                                                                               gCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAaca                                    <  7:1253375/150‑1 (MQ=255)
                                                                                                                 gTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTTGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGc                                 <  7:1421007/151‑1 (MQ=255)
                                                                                                                 gTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGc                                 <  3:1034329/151‑1 (MQ=255)
                                                                                                                  tCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCt                                >  3:1328766/1‑151 (MQ=255)
                                                                                                                   cTGCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGCTGAGCCGATATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTg                               >  4:1637509/1‑151 (MQ=255)
                                                                                                                     gCGGATGATAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGTTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGa                              <  6:1453801/150‑1 (MQ=255)
                                                                                                                        gatgatAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACAAGCTGAcc                            >  2:414500/1‑149 (MQ=255)
                                                                                                                         atgatAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACTGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAgc                         >  1:490690/1‑151 (MQ=255)
                                                                                                                          tgatAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAgct                        >  5:411711/1‑151 (MQ=255)
                                                                                                                           gatAAGGCCGGGAGTGCCCCACCCGGATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAgctg                       <  5:1236887/151‑1 (MQ=255)
                                                                                                                           gatAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAgctg                       <  8:1177811/151‑1 (MQ=255)
                                                                                                                            atAAGGCCGGGAGTGCCCCACCCGGATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAgctgc                      >  8:960741/1‑151 (MQ=255)
                                                                                                                            atAAGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAgctgc                      >  3:307368/1‑151 (MQ=255)
                                                                                                                              aaGGCCGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAgctgct                     <  8:306350/150‑1 (MQ=255)
                                                                                                                               aGGCCGGGAGTGCCCCACCCGAATACCCAGGCCCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGCTGTCGCCCCACGGTGAGCCGTTATCCATGCTCATCCGCTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAgctgctgc                   >  5:477080/1‑151 (MQ=255)
                                                                                                                                  ccGGGAGTGCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCtgctgctgc                >  5:919498/1‑151 (MQ=255)
                                                                                                                                   cGGGAGTGCCCCAGCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGACAGGCCGTAACGCTCAAACACGCTGACCAGCtgctgctgcc               >  5:756533/1‑150 (MQ=255)
                                                                                                                                          gCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGtctc        <  6:641653/151‑1 (MQ=255)
                                                                                                                                          gCCCCACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGtctc        <  3:1093512/151‑1 (MQ=255)
                                                                                                                                            cccACCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTcgc      >  7:1157785/1‑151 (MQ=255)
                                                                                                                                               aCCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGcc   >  4:1072767/1‑151 (MQ=255)
                                                                                                                                               aCCCGAATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGcc   >  2:1203103/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGGATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTGCCGGTGGTGTCGCCCCACGGTGAGCCGTTATCCATGGTCATCCGGTCCGGCAGGCCGTAACGCTCAAACACGCTGACCAGCTGCTGCTGCACGGTCTCGCGCCG  >  NC_000913/281143‑281437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑