| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,224,895 | C→T | L755L (CTG→TTG) | ebgA → | evolved beta‑D‑galactosidase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,224,895 | 0 | C | T | 96.1% | 1348.9 / 27.5 | 417 | L755L (CTG→TTG) | ebgA | evolved beta‑D‑galactosidase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (11/5); new base T (194/205); total (206/211) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01 | |||||||||||
Only 100 of 428 total aligned reads displayed.
GCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGC > NC_000913/3224747‑3225042 | gCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGctgttg < 7:812053/151‑1 (MQ=255) gTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTc > 8:653246/1‑151 (MQ=255) tGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTcg > 2:1635659/1‑151 (MQ=255) gCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTcgc < 7:171030/151‑1 (MQ=255) gCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTcgc < 8:1524341/151‑1 (MQ=255) ttCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCCATCACCTTCTCAAAAATGAGTGGCAGACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGCCTCGAGAGc > 6:1400529/1‑151 (MQ=255) gCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCaa < 3:201700/151‑1 (MQ=255) cACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCaaa < 1:1148140/151‑1 (MQ=255) cACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTATGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCAGCTTCTCAAAAATGAGTGGCAACCCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCaa > 4:1169056/1‑150 (MQ=255) aCCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCCCCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCaa > 6:29564/1‑149 (MQ=255) aCCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCaa > 8:566632/1‑149 (MQ=255) cAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCACACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCaa > 1:1199563/1‑147 (MQ=255) aataatGCGCGTCTGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCa < 3:752441/151‑1 (MQ=255) aataatGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATc > 1:1596250/1‑150 (MQ=255) aatGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAAct > 3:580195/1‑151 (MQ=255) aatGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAAct < 7:1510778/151‑1 (MQ=255) gcgcGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAActtct > 6:1198047/1‑151 (MQ=255) gTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCaa < 7:583550/151‑1 (MQ=255) ccGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGc < 1:608475/151‑1 (MQ=255) ccGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGc > 8:775331/1‑151 (MQ=255) cGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGcc > 8:1047635/1‑151 (MQ=255) gCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCg > 8:272909/1‑151 (MQ=255) aCGCTGTATGACGATCGTTTGAGCTGCACCGTTCTCGGCTACTACTTGGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATGGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCgatg < 6:402128/150‑1 (MQ=255) cGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGTCGAATAGCTGTTGAGTCGCGAGCCAAAGATCAACTTATTCAAGCCgatgat > 6:93559/1‑151 (MQ=255) cGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCgatgat > 8:1229481/1‑151 (MQ=255) gCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATc > 3:464215/1‑151 (MQ=255) gCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATc < 1:1305508/151‑1 (MQ=255) tGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACCTCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCgc > 2:1383791/1‑150 (MQ=255) ggAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGAc > 4:733137/1‑151 (MQ=255) gAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGCCTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGAc > 4:6717/1‑150 (MQ=255) aaGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACa > 4:1578643/1‑150 (MQ=255) gACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAAcc < 2:557844/151‑1 (MQ=255) aCGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAAcc > 4:397637/1‑150 (MQ=255) cGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACcac < 7:467063/151‑1 (MQ=255) cGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACcac < 8:1148177/151‑1 (MQ=255) gATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACcaca < 4:565970/151‑1 (MQ=255) cGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAg < 3:1449817/150‑1 (MQ=255) ttGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAgg > 3:125961/1‑151 (MQ=255) tGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAgg > 2:391845/1‑150 (MQ=255) gAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAg < 8:898880/151‑1 (MQ=255) gCTGCACCGTTCTCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGGAAACCGACCTCCTTGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAACATCAACTTCTTCAAGGCGATGATCGACAACCACAAGCAgtagta < 2:331925/151‑1 (MQ=255) cTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTAc > 5:1129885/1‑151 (MQ=255) gCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTAc < 4:276370/149‑1 (MQ=255) cACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGaa < 1:355935/151‑1 (MQ=255) cACCGTTCGCGGCTACAACAACGCGATCACCATCTCAAAAATGAGTGGCAAACCGACATCCTGGCACGAGAATGCCGAATCGCACAAGACTCGCGAGCCAAAGATCAACTTCTTCAACCCGATGATCGACAACCACAAGCAGGACAACGa > 2:837618/1‑150 (MQ=255) ccGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAgg > 4:797113/1‑151 (MQ=255) ccGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAgg < 2:1241153/151‑1 (MQ=255) tCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCtg > 2:1666621/1‑151 (MQ=255) tCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCt < 8:44435/150‑1 (MQ=255) gcgGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAACCCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGCCTCGCGAGCCAAAGATCAACATCTTCAAGCCGATGATCGACAACCACACCCAGGAGTCCGAAGGGCggtg > 5:1460395/1‑151 (MQ=255) gcgGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCtgtg > 5:1536137/1‑151 (MQ=255) gcgGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCtgtg > 5:1663718/1‑151 (MQ=255) ggCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGc < 2:186806/151‑1 (MQ=255) ggCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGc < 5:506772/151‑1 (MQ=255) aCAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAAcc < 1:1520580/151‑1 (MQ=255) aaCTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCg > 6:158247/1‑150 (MQ=255) aaCTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCg > 7:298219/1‑150 (MQ=255) cgcgATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGCTCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCAt > 1:879770/1‑151 (MQ=255) cgcgATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCAt < 7:1337882/151‑1 (MQ=255) gcgATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGATTCAtt > 6:1055806/1‑151 (MQ=255) gcgATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCAtt > 1:1021713/1‑151 (MQ=255) cgATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCAttt > 7:627573/1‑151 (MQ=255) cgATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCAttt > 2:1083240/1‑151 (MQ=255) gATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTg < 3:373284/151‑1 (MQ=255) aTCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGc < 1:957258/151‑1 (MQ=255) tCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCGAAAGATCAACTTCTTCAAGCCGATGATCGCGAACCTCAAGCAGGAGTCCGAAGGGCTGTGGCGCCCGAATCAGTTGcg > 2:1428699/1‑150 (MQ=255) cACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGc > 1:240546/1‑149 (MQ=255) ccTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGAt < 5:1039675/151‑1 (MQ=255) ctcAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATAAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCACCCGCATCCTTTGCAGATCATg > 2:611562/1‑151 (MQ=255) cAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCAt < 8:1452747/148‑1 (MQ=255) cAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGc < 6:788236/150‑1 (MQ=255) cAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCa < 7:730400/151‑1 (MQ=255) aaaaTGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGCGCCAAAGATCAACTTATTCACGCCGATGAACGCCACCCACAAGCAGGAGTACGAAGGCATGTGCCAACCGAATCATTTGACGATCATGCAgg > 3:885656/1‑151 (MQ=255) aaTGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGaa < 6:20824/151‑1 (MQ=255) aTGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAAc > 8:1618312/1‑151 (MQ=255) gAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACAt < 3:406880/151‑1 (MQ=255) gAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAAGCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACAt < 2:971750/151‑1 (MQ=255) cAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTgcgc < 1:451221/151‑1 (MQ=255) aaaCCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGcgcg < 2:714299/151‑1 (MQ=255) aCCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGAc < 6:1687448/151‑1 (MQ=255) gACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACttt < 1:1450480/151‑1 (MQ=255) gACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCAAAAGATCAACTTCTTCAAGCCGATGATCGACACCCACAACCAGGAGTACGAAGGGCTGTGGCAACCCAATCATTTGCAGAACATTCAGGAACATATGCGCGACttt > 4:143368/1‑151 (MQ=255) cATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGc > 1:765679/1‑151 (MQ=255) aTCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGcc < 1:497987/151‑1 (MQ=255) tCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCg > 5:304824/1‑151 (MQ=255) tGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGt > 1:1060219/1‑149 (MQ=255) ggCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAg < 3:1581546/150‑1 (MQ=255) ggCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGa < 3:989709/151‑1 (MQ=255) ggCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGa < 7:1102876/151‑1 (MQ=255) aGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCACGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACa < 2:780505/151‑1 (MQ=255) ggTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACag < 1:1678425/151‑1 (MQ=255) gAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACagag < 5:765514/150‑1 (MQ=255) gAATGGCGAATCGCTGCTGACTTGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGc > 3:1158421/1‑151 (MQ=255) aaTGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGca < 7:1029979/151‑2 (MQ=255) aTGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCg > 6:49360/1‑150 (MQ=255) tGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGAt > 5:1602610/1‑151 (MQ=255) gAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTg > 2:154997/1‑151 (MQ=255) aaTCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCTCGACTTTGCCGTAGAACAGAGCGATGGTg > 5:613557/1‑150 (MQ=255) cGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGt > 6:1521475/1‑151 (MQ=255) cGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGt > 5:874763/1‑151 (MQ=255) | GCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGC > NC_000913/3224747‑3225042 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |