BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,227,339	C→T	T369I (ACC→ATC)	ygjI →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,227,339	0	C	T	97.3%	1261.5 / 13.8	374	T369I (ACC→ATC)	ygjI	putative transporter
Reads supporting (aligned to +/- strand): ref base C (1/9); new base T (200/163); total (202/172)
Fisher's exact test for biased strand distribution p-value = 6.87e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

Only 100 of 392 total aligned reads displayed.

TTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATATTTGCCG  >  NC_000913/3227191‑3227488

tttGGTAAGAAAACCGTCGAGCTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATCAATACTATTATTAATATGAtcg                                                                                                                                                     >  6:1126594/1‑151 (MQ=255)

 ttGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGTTCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAtcg                                                                                                                                                     <  8:1607089/150‑1 (MQ=255)

 ttGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAtcg                                                                                                                                                     <  8:620390/150‑1 (MQ=255)

 ttGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAtcg                                                                                                                                                     <  4:810270/150‑1 (MQ=255)

  tcgTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTTATGAATACTATTATAAATATGAtcgcc                                                                                                                                                   >  6:602237/3‑151 (MQ=255)

   ggTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAtcgccg                                                                                                                                                  <  1:78493/151‑1 (MQ=255)

   ggTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAccgccg                                                                                                                                                  >  5:622948/1‑151 (MQ=255)

    gTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATcgccgc                                                                                                                                                 <  3:1561281/151‑1 (MQ=255)

       aGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGc                                                                                                                                              <  8:862977/151‑1 (MQ=255)

                 cGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCtt                                                                                                                                    <  7:597589/151‑1 (MQ=255)

                 cGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCt                                                                                                                                     <  3:1263099/150‑1 (MQ=255)

                   aaCTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGGGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTcc                                                                                                                                  <  7:472762/151‑1 (MQ=255)

                      tGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTccgcc                                                                                                                               >  7:1630224/1‑151 (MQ=255)

                       gAACGAAACCGGCGGTCCGGCGCGCGCAGCGTGGAGCCAGTTCCTGATCGTCATCCGGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTccgc                                                                                                                                <  6:806106/149‑1 (MQ=255)

                       gAACGAAAACGGCGTTCCGGCGCGCGCAGCGGGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTACAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTccgccg                                                                                                                              <  4:522115/151‑1 (MQ=255)

                        aaCGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCCTGCTTCCGCCGt                                                                                                                             >  5:1190292/1‑151 (MQ=255)

                         aCGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGtt                                                                                                                            <  1:1327823/151‑1 (MQ=255)

                             aaaCGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATtc                                                                                                                        <  3:1210766/151‑1 (MQ=255)

                                 ggCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCATTACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCGGTTATtcatc                                                                                                                     >  2:1167879/1‑150 (MQ=255)

                                  gCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTcatcat                                                                                                                   >  4:440206/1‑151 (MQ=255)

                                        ccgcgcgcgcAGCGTGGATCCAGTTCCTCATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGAGCAGCATCTGATGAATACTATTATTAATATGAACGCCGCAGCGACCATGCTTCCGCCGTTATACATCAAGCTGGc                                                                                                             >  4:1081088/3‑151 (MQ=255)

                                        cGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGc                                                                                                             >  7:368658/1‑151 (MQ=255)

                                          gcgcgcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGTATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCtt                                                                                                           <  6:720602/151‑1 (MQ=255)

                                           cgcgcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCtt                                                                                                           >  8:1320098/1‑150 (MQ=255)

                                           cgcgcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTa                                                                                                          <  8:1017770/151‑1 (MQ=255)

                                            gcgcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTAc                                                                                                         >  3:1447115/1‑151 (MQ=255)

                                             cgcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTAcc                                                                                                        <  5:838786/151‑1 (MQ=255)

                                              gcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCt                                                                                                       >  2:543004/1‑151 (MQ=255)

                                                 cAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGaa                                                                                                    <  5:478344/151‑1 (MQ=255)

                                                  aGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAAt                                                                                                   >  6:487216/1‑151 (MQ=255)

                                                  aGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAAt                                                                                                   >  4:788777/1‑151 (MQ=255)

                                                   gCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAAtt                                                                                                  <  1:1156967/151‑1 (MQ=255)

                                                   gCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATAAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAAtt                                                                                                  >  2:991213/1‑151 (MQ=255)

                                                    cGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAAttt                                                                                                 <  5:111608/151‑1 (MQ=255)

                                                    cGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAAttt                                                                                                 >  6:853273/1‑151 (MQ=255)

                                                     gTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTa                                                                                                <  7:1335452/151‑1 (MQ=255)

                                                      tGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTAc                                                                                               >  6:1527180/1‑151 (MQ=255)

                                                       ggATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTAcc                                                                                              >  1:1432886/1‑150 (MQ=255)

                                                        gATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTAcgc                                                                                             <  5:1524068/151‑1 (MQ=255)

                                                         aTCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTAcgcc                                                                                            >  2:706208/1‑150 (MQ=255)

                                                           ccAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGcc                                                                                          <  4:1651257/151‑1 (MQ=255)

                                                             aGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCa                                                                                         >  5:746505/1‑150 (MQ=255)

                                                               ttCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAAt                                                                                      >  4:649817/1‑151 (MQ=255)

                                                                tCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAAt                                                                                      <  6:384162/150‑1 (MQ=255)

                                                                    gATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAg                                                                                   <  6:202299/149‑1 (MQ=255)

                                                                    gATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGAt                                                                                 <  2:1128747/151‑1 (MQ=255)

                                                                    gATCGTCAACCCGCTGCTGAATATCCCGATGCTCCGTTCCAAAACCGTGCACGATCTGATGAATACTAATAATAATAAGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGAt                                                                                 <  1:338770/151‑1 (MQ=255)

                                                                     aTCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATc                                                                                <  8:459219/151‑1 (MQ=255)

                                                                      tCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATc                                                                                >  7:1654783/1‑150 (MQ=255)

                                                                       cGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCa                                                                               <  8:1422226/150‑1 (MQ=255)

                                                                           aTCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGc                                                                          >  7:826987/1‑151 (MQ=255)

                                                                             cccGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCa                                                                        <  8:1691126/151‑1 (MQ=255)

                                                                              ccGCTGATGATTATCCCGATGCTCAGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCAc                                                                       >  3:839089/1‑151 (MQ=255)

                                                                               cGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCAcg                                                                      <  2:484450/151‑1 (MQ=255)

                                                                               cGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCAcg                                                                      <  4:1028792/151‑1 (MQ=255)

                                                                                  tgatgaTTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCAcgcg                                                                    >  4:1558283/1‑150 (MQ=255)

                                                                                   gatgatTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGa                                                                   <  2:224648/150‑1 (MQ=255)

                                                                                       atTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTcc                                                              <  2:1389565/151‑1 (MQ=255)

                                                                                          aTCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGt                                                            >  1:188617/1‑150 (MQ=255)

                                                                                           tCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGACACGCGATTTCCGTAt                                                          >  6:1655951/1‑151 (MQ=255)

                                                                                             ccGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATgg                                                        >  5:1251166/1‑151 (MQ=255)

                                                                                             ccGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATgg                                                        <  6:1398042/151‑1 (MQ=255)

                                                                                                aTGCTCGGTTCCAATACCGTGCAGGATCTGATGACTACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCAGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCt                                                     <  1:961227/151‑1 (MQ=255)

                                                                                                aTGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCt                                                     <  7:574450/151‑1 (MQ=255)

                                                                                                aTGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCt                                                     <  5:1574977/151‑1 (MQ=255)

                                                                                                aTGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCAGCAGAGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCt                                                     >  2:863919/1‑151 (MQ=255)

                                                                                                    tCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCccg                                                 >  7:1420930/1‑151 (MQ=255)

                                                                                                     cGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCccgc                                                <  3:777247/151‑1 (MQ=255)

                                                                                                        ttCCAATACCGTGCAGGATCTGATGAATACTATTATTAATAAGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCcgccgc                                             >  1:706674/1‑151 (MQ=255)

                                                                                                           cAATACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCAcc                                          >  3:627143/1‑151 (MQ=255)

                                                                                                            aaTACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCg                                         <  3:1158029/151‑1 (MQ=255)

                                                                                                            aaTACCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTACCGCCGCACCg                                         >  2:132678/1‑151 (MQ=255)

                                                                                                               aCCGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGt                                       >  7:1267549/1‑150 (MQ=255)

                                                                                                                ccGTGCAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGtat                                     <  4:264348/151‑1 (MQ=255)

                                                                                                                     cAGGATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCg                                >  8:759238/1‑151 (MQ=255)

                                                                                                                        gATCTGATGAATACTATTATTAATATGATCGCCGCAGCGTCCCTGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCgttg                             >  4:1427842/1‑151 (MQ=255)

                                                                                                                             gatgaATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTc                         >  5:1363535/1‑150 (MQ=255)

                                                                                                                             gatgaATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCa                        <  1:162812/151‑1 (MQ=255)

                                                                                                                               tgaATACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAAt                      >  8:1107020/1‑151 (MQ=255)

                                                                                                                                gaATACTATTATTAATATGCTCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACATGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATg                     <  7:669871/151‑1 (MQ=255)

                                                                                                                                 aaTACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGc                    >  1:1279036/1‑151 (MQ=255)

                                                                                                                                 aaTACTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGc                    >  6:1272629/1‑151 (MQ=255)

                                                                                                                                    accattattAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTg                  <  1:461051/147‑1 (MQ=255)

                                                                                                                                    aCTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTg                  <  7:863432/150‑1 (MQ=255)

                                                                                                                                    aCTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTg                  >  2:1485409/1‑150 (MQ=255)

                                                                                                                                     cTATTATTAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGAt                >  4:1231701/1‑151 (MQ=255)

                                                                                                                                       attattAATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTg              >  2:165503/1‑151 (MQ=255)

                                                                                                                                         tcttaATATGCTCGCCGCAGCGGCCCTGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGc             <  6:1311907/148‑1 (MQ=255)

                                                                                                                                         tattaATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCg            >  7:1113298/1‑151 (MQ=255)

                                                                                                                                            taATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGat          >  4:916425/1‑150 (MQ=255)

                                                                                                                                            taATATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGat          >  6:282358/1‑150 (MQ=255)

                                                                                                                                             aaTATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGatat        >  5:977986/1‑151 (MQ=255)

                                                                                                                                             aaTATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGatat        <  8:1066038/151‑1 (MQ=255)

                                                                                                                                             aaTATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGatat        <  3:240927/151‑1 (MQ=255)

                                                                                                                                             aaTATGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGata         <  8:1368184/150‑1 (MQ=255)

                                                                                                                                              atatGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATAtt       >  5:322758/1‑151 (MQ=255)

                                                                                                                                                atGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATATTTg     >  8:294205/1‑151 (MQ=255)

                                                                                                                                                atGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATATTTg     <  7:1207701/151‑1 (MQ=255)

                                                                                                                                                 tGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATATTTGc    >  3:511427/1‑151 (MQ=255)

                                                                                                                                                 tGATCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATATTTGc    >  1:1032490/1‑151 (MQ=255)

TTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATATTTGCCG  >  NC_000913/3227191‑3227488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`