Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,506,393 T→C D315G (GAC→GGC)  yhfT ← inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,506,3930TC95.5% 1411.5 / 26.7 404D315G (GAC→GGC) yhfTinner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (9/9);  new base C (191/194);  total (201/203)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01

Only 100 of 408 total aligned reads displayed.

ATTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGTCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTT  >  NC_000913/3506245‑3506539
                                                                                                                                                    |                                                                                                                                                  
  tCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCAc                                                                                                                                                >  5:979731/1‑151 (MQ=255)
   cATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCAc                                                                                                                                                <  2:1137629/150‑1 (MQ=255)
    aTGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTCCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCg                                                                                                                                              >  3:340146/1‑151 (MQ=255)
      ggCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGc                                                                                                                                             <  5:85231/150‑1 (MQ=255)
        cGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCAt                                                                                                                                           >  4:1609634/1‑150 (MQ=255)
        cGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATa                                                                                                                                          <  6:1219736/151‑1 (MQ=255)
         gTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATaa                                                                                                                                         <  1:512211/151‑1 (MQ=255)
           tACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATaaa                                                                                                                                        <  1:1273484/150‑1 (MQ=255)
              ggATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACaa                                                                                                                                     >  8:1642013/1‑150 (MQ=255)
                   ttATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTa                                                                                                                                >  5:1127547/1‑150 (MQ=255)
                   ttATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTa                                                                                                                                <  5:206969/150‑1 (MQ=255)
                    tATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTaa                                                                                                                               <  7:727680/150‑1 (MQ=255)
                    tATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTaa                                                                                                                               >  2:1414481/1‑150 (MQ=255)
                    tATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTaa                                                                                                                               >  6:1098168/1‑150 (MQ=255)
                     aTCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTaaa                                                                                                                              <  5:92695/150‑1 (MQ=255)
                     aTCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAg                                                                                                                             <  6:657552/151‑1 (MQ=255)
                         gACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCg                                                                                                                         <  5:536899/151‑1 (MQ=255)
                         gACGCATTACGCACCGACGGGTAGAGTCCCCCCCAGTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGACATAGCCCACCTCATAAACAAAGGTAAAGcccc                                                                                                                         >  6:1506621/1‑150 (MQ=255)
                         gACGCATTACGCACCGACGCGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGCATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGcccc                                                                                                                         >  6:462234/1‑150 (MQ=255)
                              aTTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACt                                                                                                                    >  4:123766/1‑151 (MQ=255)
                                tACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTg                                                                                                                   <  7:1349826/150‑1 (MQ=255)
                                tACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTg                                                                                                                   <  5:1461927/150‑1 (MQ=255)
                                  cGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCAt                                                                                                                >  1:218806/1‑151 (MQ=255)
                                  cGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCAt                                                                                                                <  3:1168322/151‑1 (MQ=255)
                                    cACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATac                                                                                                              >  6:358787/1‑151 (MQ=255)
                                     aCCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATac                                                                                                              >  5:1649843/1‑150 (MQ=255)
                                        gACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGTCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATacacac                                                                                                          >  8:451003/1‑151 (MQ=255)
                                           gggTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCg                                                                                                       <  3:605364/151‑1 (MQ=255)
                                              tAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGTCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTg                                                                                                    >  4:1083381/1‑151 (MQ=255)
                                               aGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGc                                                                                                   >  2:156881/1‑151 (MQ=255)
                                                 cGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTa                                                                                                 <  6:394892/151‑1 (MQ=255)
                                                   tCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAAc                                                                                               >  1:9256/1‑151 (MQ=255)
                                                    cccAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAAc                                                                                               <  8:666731/150‑1 (MQ=255)
                                                    cccAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAAc                                                                                               <  1:1329295/150‑1 (MQ=255)
                                                     ccAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATACCCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAAcgc                                                                                             <  1:999866/151‑1 (MQ=255)
                                                          cATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCggtgg                                                                                        <  6:770892/151‑1 (MQ=255)
                                                               gCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGc                                                                                    >  2:865337/1‑150 (MQ=255)
                                                                  gATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATc                                                                                <  8:114/151‑1 (MQ=255)
                                                                  gATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATc                                                                                <  8:365683/151‑1 (MQ=255)
                                                                     cgaacgaaGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCaa                                                                              <  3:1237255/150‑1 (MQ=255)
                                                                      gaccgaaGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACg                                                                            <  4:293338/148‑1 (MQ=255)
                                                                       aacgaaGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACgg                                                                           <  2:1325927/151‑1 (MQ=255)
                                                                          gaaGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGcac                                                                        >  4:371197/1‑151 (MQ=255)
                                                                          gaaGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGcac                                                                        <  3:207407/151‑1 (MQ=255)
                                                                           aaGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGcac                                                                        >  6:957403/1‑150 (MQ=255)
                                                                              cagcaAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAAc                                                                    <  8:1316819/151‑1 (MQ=255)
                                                                              cagcaAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAAc                                                                    <  2:683615/151‑1 (MQ=255)
                                                                              cagcaAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAAc                                                                    <  4:998479/151‑1 (MQ=255)
                                                                                 caAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAAcccc                                                                 <  3:1668557/151‑1 (MQ=255)
                                                                                 caAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAAcccc                                                                 >  4:673988/1‑151 (MQ=255)
                                                                                   aGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAg                                                               <  7:827743/151‑1 (MQ=255)
                                                                                      cTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTcc                                                            >  1:1528880/1‑151 (MQ=255)
                                                                                      cTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGGAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTcc                                                            >  1:773103/1‑151 (MQ=255)
                                                                                        tCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTcc                                                            <  5:314587/149‑1 (MQ=255)
                                                                                        tCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTcc                                                            <  2:169452/149‑1 (MQ=255)
                                                                                        tCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCcg                                                           <  7:939238/150‑1 (MQ=255)
                                                                                           gccgAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAACCCCCAGTCCgcgc                                                        >  7:1667293/1‑150 (MQ=255)
                                                                                            ccgAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCAt                                                      >  7:618613/1‑151 (MQ=255)
                                                                                            ccgAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCAt                                                      <  7:536274/151‑1 (MQ=255)
                                                                                             cgAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCAt                                                      >  1:484623/1‑150 (MQ=255)
                                                                                              caaaTAACCACTGCGCCTAATACCGCTCCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATaa                                                    <  2:315639/150‑1 (MQ=255)
                                                                                               aaaTAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATaaa                                                   <  5:1342987/151‑1 (MQ=255)
                                                                                               aaaTAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATaaa                                                   <  8:50898/151‑1 (MQ=255)
                                                                                                aaTAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAAt                                                  >  2:984056/1‑151 (MQ=255)
                                                                                                aaTAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAAt                                                  >  4:706909/1‑151 (MQ=255)
                                                                                                aaTAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACACCAGTCCGCGCATAAAt                                                  >  6:1646214/1‑151 (MQ=255)
                                                                                                     ccACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGc                                             <  4:909903/151‑1 (MQ=255)
                                                                                                      cACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGcc                                            >  5:120407/1‑151 (MQ=255)
                                                                                                          gcgcCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCagag                                        <  1:1499807/151‑1 (MQ=255)
                                                                                                          gcgcCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCag                                          <  1:428112/149‑1 (MQ=255)
                                                                                                           cgcCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAgc                                       >  4:488002/1‑151 (MQ=255)
                                                                                                            gcCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAgcc                                      <  6:127611/151‑1 (MQ=255)
                                                                                                             ccTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAgccg                                     <  7:1239435/151‑1 (MQ=255)
                                                                                                             ccTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAgccg                                     <  2:1208956/151‑1 (MQ=255)
                                                                                                                  tACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTg                                 >  3:1093780/1‑150 (MQ=255)
                                                                                                                    ccGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGAtt                              <  1:100370/151‑1 (MQ=255)
                                                                                                                    ccGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGAtt                              <  1:290594/151‑1 (MQ=255)
                                                                                                                      gCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTa                             >  3:1595587/1‑150 (MQ=255)
                                                                                                                       cTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAAt                           >  8:785291/1‑151 (MQ=255)
                                                                                                                        tGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATc                          >  3:1458690/1‑151 (MQ=255)
                                                                                                                          cAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATc                          >  3:1297526/1‑149 (MQ=255)
                                                                                                                          cAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAg                        <  8:1591442/151‑1 (MQ=255)
                                                                                                                          cAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAg                        <  7:989557/151‑1 (MQ=255)
                                                                                                                           aaCCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGc                       <  6:1416844/151‑1 (MQ=255)
                                                                                                                                 cGGATTCGGCTAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTTCATAAACACCCGTTGCTAACGCGGTTGTGGCAATCAACGGCACAAACCCCAGTCCTCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGc                 <  7:426168/151‑1 (MQ=255)
                                                                                                                                 cGGATTCGGCGAGAGATAGTCCACCGCATAAACAAAGGTAAAGCCCGCTACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCGCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTg                  <  4:569904/150‑1 (MQ=255)
                                                                                                                                 cGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTg                  <  3:401444/150‑1 (MQ=255)
                                                                                                                                 cGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGc                 >  8:192582/1‑151 (MQ=255)
                                                                                                                                 cGGATTCGGCGAGAGATAGCCCAACGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTg                  <  8:893232/150‑1 (MQ=255)
                                                                                                                                   gATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCg                >  3:662437/1‑150 (MQ=255)
                                                                                                                                      tCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTg            >  5:428406/1‑151 (MQ=255)
                                                                                                                                        ggCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACCCACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGtt          >  4:1466860/1‑151 (MQ=255)
                                                                                                                                           gagagaTAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCg       >  3:285729/1‑151 (MQ=255)
                                                                                                                                           gagagaTAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCg       >  7:292819/1‑151 (MQ=255)
                                                                                                                                            agagaTAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCgg      <  5:725504/151‑1 (MQ=255)
                                                                                                                                            agagaTAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCgg      >  6:856226/1‑151 (MQ=255)
                                                                                                                                             gagaTAGCCCACCGCATATAGAAAGGTAAAGCCCGCAAGTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGc     <  2:620134/151‑1 (MQ=255)
                                                                                                                                             gagaTAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGc     >  8:1248918/1‑151 (MQ=255)
                                                                                                                                               gaTAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGCCACAAACCCCAGTCCGCCCATAAATTCTCCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGt   >  2:398360/1‑151 (MQ=255)
                                                                                                                                                aTAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGtt  >  6:211650/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ATTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGTCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTT  >  NC_000913/3506245‑3506539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑