Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,558,291 C→T G8G (GGC→GGT rtcR → sigma 54‑dependent transcriptional regulator of rtcBA expression

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,558,2910CT97.7% 1459.7 / 11.9 433G8G (GGC→GGTrtcRsigma 54‑dependent transcriptional regulator of rtcBA expression
Reads supporting (aligned to +/- strand):  ref base C (5/5);  new base T (230/191);  total (236/197)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.59e-01

Only 100 of 460 total aligned reads displayed.

ATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGCTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGA  >  NC_000913/3558143‑3558437
                                                                                                                                                    |                                                                                                                                                  
   agcagcTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGt                                                                                                                                               >  2:458354/1‑151 (MQ=255)
   agcagcTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGt                                                                                                                                               <  6:180828/151‑1 (MQ=255)
    gcagcTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACACTGCCTTTTGGTTTTGTc                                                                                                                                              >  6:219509/1‑151 (MQ=255)
      agcTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCgg                                                                                                                                            <  4:1224084/151‑1 (MQ=255)
       gcTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGt                                                                                                                                           >  7:679147/1‑151 (MQ=255)
       ccTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGAATTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGt                                                                                                                                           <  4:304023/150‑1 (MQ=255)
            aTTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGt                                                                                                                                      >  1:1589013/1‑151 (MQ=255)
             ttttttAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCTGTACCGt                                                                                                                                      >  1:1641514/1‑150 (MQ=255)
              tttttAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTa                                                                                                                                     <  5:170436/150‑1 (MQ=255)
              tttttAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTAc                                                                                                                                    >  4:1145623/1‑151 (MQ=255)
               ttttAATTCATTTTTTTAAAAAAGATTATATCTTTACGTACGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTAc                                                                                                                                    <  1:1263695/150‑1 (MQ=255)
                tttAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACt                                                                                                                                   <  4:1388328/150‑1 (MQ=255)
                tttAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTg                                                                                                                                  >  3:303226/1‑151 (MQ=255)
                    aTTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGAtt                                                                                                                              >  5:1222988/1‑151 (MQ=255)
                    aTTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGAtt                                                                                                                              >  3:1384071/1‑151 (MQ=255)
                    aTTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATAGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGAtt                                                                                                                              >  2:1029494/1‑151 (MQ=255)
                     ttCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGAtt                                                                                                                              >  3:1447689/1‑150 (MQ=255)
                              ttAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGgc                                                                                                                     <  4:1535041/150‑1 (MQ=255)
                                aaaaaaGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGcgcg                                                                                                                  >  7:1531171/1‑151 (MQ=255)
                                 aaaaaGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCgg                                                                                                                 <  3:1589427/151‑1 (MQ=255)
                                 aaaaaGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCgg                                                                                                                 >  5:512659/1‑151 (MQ=255)
                                     aGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGt                                                                                                             >  2:1468706/1‑151 (MQ=255)
                                     aGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGt                                                                                                             >  6:464953/1‑151 (MQ=255)
                                     aGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCAAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCTTACTGGATTATGAAGGGCGCGTCAGt                                                                                                             >  4:41904/1‑151 (MQ=255)
                                      gATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAACGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTc                                                                                                            <  7:796282/151‑1 (MQ=255)
                                       aTTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTc                                                                                                            >  3:193213/1‑150 (MQ=255)
                                       aTTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCa                                                                                                           <  6:780452/151‑1 (MQ=255)
                                       aTTATATCTTTACGTCCGTAACCGGAGATATCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTATATAAGGATATCTAAGATGCGTAAAACACTGGCATTTGGTTTTGTCGGTACCGTACTGGAATATGCCGGCCGCGGCAGTc                                                                                                            >  4:1675662/1‑150 (MQ=255)
                                        ttATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTc                                                                                                            >  2:1615899/1‑149 (MQ=255)
                                             tCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGAAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGGCGGGCGCGGCAGTCAGCGCTg                                                                                                     >  6:379264/1‑151 (MQ=255)
                                              cTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTATGGATTTCTCGGTACCGTACTGGATTATGCCGGGCGCGGCACTCACCGCTgg                                                                                                    >  1:961709/1‑151 (MQ=255)
                                                ttACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCt                                                                                                      <  5:1019346/147‑1 (MQ=255)
                                                 tACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTc                                                                                                  >  6:129239/1‑150 (MQ=255)
                                                  aCGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCaa                                                                                                <  2:1076511/151‑1 (MQ=255)
                                                  aCGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCa                                                                                                 >  6:1473638/1‑150 (MQ=255)
                                                   cGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGCTCaa                                                                                                >  7:1386692/1‑150 (MQ=255)
                                                    gTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCaaa                                                                                               >  1:393251/1‑150 (MQ=255)
                                                       cGTAACCGGAGATTTCCCGCAAAGACAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGGTGGTCAAAATgg                                                                                           >  8:756234/1‑151 (MQ=255)
                                                          aaCCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGt                                                                                        >  7:1273303/1‑151 (MQ=255)
                                                            ccGGAGATTTCCCGCCAAGCCCATTTACCGATAATGAACTCTCGTCTTTTATAAGGATATCTAAGATGCGTAACACAGTGGCTTTTGGTTTTGGCGGTACCGTACGGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGGcc                                                                                      <  5:853483/151‑1 (MQ=255)
                                                            ccGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTcc                                                                                      <  7:196043/151‑1 (MQ=255)
                                                              ggAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCGGGTCAAAATGGCGTCCgc                                                                                    >  2:774929/1‑150 (MQ=255)
                                                               gagaTTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGac                                                                                   >  5:1062010/1‑151 (MQ=255)
                                                                 gaTTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGacac                                                                                 >  8:1215384/1‑151 (MQ=255)
                                                                  aTTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACAct                                                                                >  7:1631576/1‑151 (MQ=255)
                                                                  aTTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACAct                                                                                <  1:500392/151‑1 (MQ=255)
                                                                     tCCAGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACActct                                                                              <  5:673298/150‑1 (MQ=255)
                                                                         gCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGttt                                                                          >  8:644587/1‑150 (MQ=255)
                                                                         gCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGCCGTCCGACACTCTGttt                                                                          >  3:589266/1‑150 (MQ=255)
                                                                            aaGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAACCAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGGCAAAATGGCGTCCGACACTCTGTTTATGc                                                                      <  6:559417/151‑1 (MQ=255)
                                                                             aGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATgac                                                                     <  1:217294/151‑3 (MQ=255)
                                                                             aGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGcc                                                                     >  6:712001/1‑151 (MQ=255)
                                                                               ccAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAg                                                                   <  5:927012/151‑1 (MQ=255)
                                                                               ccAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAg                                                                   <  6:1593822/151‑1 (MQ=255)
                                                                                     tACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCAGGGAGCTGCAGTCAGCGCTGGTCAAAATGGCATCCGCCACTCTGTTTATGCCAGCAAGa                                                              >  6:891720/1‑150 (MQ=255)
                                                                                       ccGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATc                                                           <  5:962186/151‑1 (MQ=255)
                                                                                       ccGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCGTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATc                                                           <  6:1060438/151‑1 (MQ=255)
                                                                                        cGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCg                                                          >  8:1504431/1‑151 (MQ=255)
                                                                                            aaTGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTg                                                       <  1:1021869/150‑1 (MQ=255)
                                                                                               gAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTc                                                    >  2:506155/1‑150 (MQ=255)
                                                                                               gAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGAGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGACAGCAAGAATCGTTGGTc                                                    >  2:58075/1‑150 (MQ=255)
                                                                                                   tatCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCatcg                                                >  3:1141544/1‑150 (MQ=255)
                                                                                                    atCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCatcgat                                              >  7:44079/1‑151 (MQ=255)
                                                                                                    atCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCatcga                                               <  3:1000983/150‑1 (MQ=255)
                                                                                                    atCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCatcga                                               <  1:676154/150‑1 (MQ=255)
                                                                                                      cGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCatcgatcg                                            <  7:497599/151‑1 (MQ=255)
                                                                                                        tCTTTTATAAGGATATCTAAGATGTGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGAc                                          >  7:1156677/1‑151 (MQ=255)
                                                                                                         gttttATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTGGGTGATCGATCGACt                                         >  8:1271245/2‑151 (MQ=255)
                                                                                                         cTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACt                                         >  8:1678539/1‑151 (MQ=255)
                                                                                                          ttttATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAACAAGAATCGTTGGTCATCGATCGACTg                                        >  5:1346188/1‑151 (MQ=255)
                                                                                                              ataAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGaa                                     <  1:1420435/150‑1 (MQ=255)
                                                                                                                aaGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGCTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAAttg                                  <  1:751944/151‑1 (MQ=255)
                                                                                                                 aGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAAttgt                                 >  2:229258/1‑151 (MQ=255)
                                                                                                                 aGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATAGATCGACTGGAAttgt                                 >  7:935103/1‑151 (MQ=255)
                                                                                                                  ggATATCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATAGATCGACTGGAAttgtt                                >  2:1353149/1‑151 (MQ=255)
                                                                                                                     tatCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGc                              >  1:1118334/1‑150 (MQ=255)
                                                                                                                      atCTAAGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTCGTCAAAATGGCGTCCGACACTCTGTTTAGGCCAGCAAGAATCGTTGGTCATCGATAGACTGGAATTGTTGCac                            >  8:413598/1‑151 (MQ=255)
                                                                                                                          aaGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCacgacg                        <  1:31603/151‑1 (MQ=255)
                                                                                                                          aaGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCacgacc                        >  8:1458286/1‑150 (MQ=255)
                                                                                                                          aaGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCacg                           <  4:1581962/148‑1 (MQ=255)
                                                                                                                          aaGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCacg                           <  5:1450263/148‑1 (MQ=255)
                                                                                                                          aaGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCacg                           <  5:233907/148‑1 (MQ=255)
                                                                                                                          aaGATGCGTAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCacg                           <  2:180062/148‑1 (MQ=255)
                                                                                                                          aaGATGCGTAAAACAGTGGCTTTTGGCTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCacgacg                        <  7:131806/151‑1 (MQ=255)
                                                                                                                             aTGCGTAAAACAGTGGCTTTTGGCTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGccc                     >  4:1263088/1‑151 (MQ=255)
                                                                                                                                  tAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCcgctcg                >  7:343525/1‑151 (MQ=255)
                                                                                                                                  tAAAACAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCcgctcg                >  3:670312/1‑151 (MQ=255)
                                                                                                                                   aaaaCAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCcgctcg                <  3:1125125/150‑1 (MQ=255)
                                                                                                                                    aaaCAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGGGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCcgctcgcg              <  2:414315/151‑1 (MQ=255)
                                                                                                                                    aaaCAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCcgctcgcg              >  8:583764/1‑151 (MQ=255)
                                                                                                                                    aaaCAGTGGCTTTTGGCTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCcgctcgcg              <  4:647950/151‑1 (MQ=255)
                                                                                                                                     aaCAGTTGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATAGATCGACTGGAATTGTTGCACGACGCCcgctcgcgc             <  8:240501/151‑1 (MQ=255)
                                                                                                                                      aCAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTTGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCcgctcgcgct            <  3:1533192/151‑1 (MQ=255)
                                                                                                                                       cAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGTGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCcgctcgcgctc           <  3:868341/151‑1 (MQ=255)
                                                                                                                                       cAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCcgctcgcgctc           <  3:455877/151‑1 (MQ=255)
                                                                                                                                       cAGTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCcgctcgcgctc           <  4:593628/151‑1 (MQ=255)
                                                                                                                                         gTGGCTTTTGGTTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCgctcgcgctcgc         <  5:406616/151‑1 (MQ=255)
                                                                                                                                             cttttggttttGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTAtt     >  7:1208121/1‑151 (MQ=255)
                                                                                                                                               tttggttttGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTAtt     <  3:1047012/149‑1 (MQ=255)
                                                                                                                                                ttggttttGTGGGTAGCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTg   <  7:159672/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGCTTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGA  >  NC_000913/3558143‑3558437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: