Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,585,517 G→A A436V (GCC→GTC)  ggt ← gamma‑glutamyltranspeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,585,5170GA97.3% 1477.2 / 16.1 441A436V (GCC→GTC) ggtgamma‑glutamyltranspeptidase
Reads supporting (aligned to +/- strand):  ref base G (8/4);  new base A (213/214);  total (222/219)
Fisher's exact test for biased strand distribution p-value = 3.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 441 total aligned reads displayed.

GCTACCGCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCT  >  NC_000913/3585369‑3585664
                                                                                                                                                    |                                                                                                                                                   
   aCCGCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACgg                                                                                                                                                  <  7:1379145/149‑1 (MQ=255)
     cGCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGgaga                                                                                                                                               >  7:314903/1‑150 (MQ=255)
     cGCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGaa                                                                                                                                              <  2:278935/151‑1 (MQ=255)
     cGCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGaa                                                                                                                                              <  5:586700/151‑1 (MQ=255)
      gCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGGCGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGaaa                                                                                                                                             <  1:1514742/151‑1 (MQ=255)
      gCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGaa                                                                                                                                              >  6:610367/1‑150 (MQ=255)
       ccTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAat                                                                                                                                            >  1:1399512/1‑151 (MQ=255)
        cTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAatc                                                                                                                                           >  1:529923/1‑151 (MQ=255)
          gggCTACCGGTCACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAatcat                                                                                                                                         <  4:1667655/151‑1 (MQ=255)
          gggCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAatcat                                                                                                                                         >  2:1287018/1‑151 (MQ=255)
           ggCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAatcatc                                                                                                                                        <  6:1267373/151‑1 (MQ=255)
            gCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATcatcc                                                                                                                                       >  3:1674254/1‑151 (MQ=255)
              tACCGGTAACCAGCCAGGTTTTACCGTATTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATcatccat                                                                                                                                     >  5:771914/1‑151 (MQ=255)
                ccGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCt                                                                                                                                   <  7:1229215/151‑1 (MQ=255)
                   gTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGt                                                                                                                                <  6:265230/151‑1 (MQ=255)
                   gTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGt                                                                                                                                <  3:193069/151‑1 (MQ=255)
                   gTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGt                                                                                                                                <  3:1325440/151‑1 (MQ=255)
                     aaCCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGtt                                                                                                                               <  6:774659/150‑1 (MQ=255)
                     aaCCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGtt                                                                                                                               <  5:108467/150‑1 (MQ=255)
                     aaCCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGtt                                                                                                                               <  8:76931/150‑1 (MQ=255)
                       ccagccagGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGttatt                                                                                                                            >  5:256379/1‑151 (MQ=255)
                       ccagccagGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGttatt                                                                                                                            <  1:420529/151‑1 (MQ=255)
                       ccagccagGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGttatt                                                                                                                            <  3:3601/151‑1 (MQ=255)
                          gccagGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGttatta                                                                                                                           >  6:1524812/1‑149 (MQ=255)
                           ccagGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGc                                                                                                                        <  6:134712/151‑1 (MQ=255)
                           ccagGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGc                                                                                                                        <  3:1261170/151‑1 (MQ=255)
                            cagGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGc                                                                                                                        >  5:1026679/1‑150 (MQ=255)
                            cagGCTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCa                                                                                                                       <  1:319715/151‑1 (MQ=255)
                             agGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGc                                                                                                                        >  3:1140472/1‑149 (MQ=255)
                             agGTTTTACCGTCTTTCACCACAATGGGCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAg                                                                                                                      <  5:39931/151‑1 (MQ=255)
                              ggTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGc                                                                                                                        >  3:695137/1‑148 (MQ=255)
                              ggTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGa                                                                                                                     <  3:1560200/151‑1 (MQ=255)
                                ttttACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAAt                                                                                                                   >  3:287902/1‑151 (MQ=255)
                                 tttACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTAGACGGAGAAATCATCCATCTGGTTATTAAGCAGAAt                                                                                                                   >  6:837679/1‑150 (MQ=255)
                                  ttACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATa                                                                                                                  <  2:69083/150‑1 (MQ=255)
                                  ttACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATAc                                                                                                                 >  2:36240/1‑151 (MQ=255)
                                    aCCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACTGCGTTGGCATCAGCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGATAAATCATCCATCTGGTTATTAAGCAGAATAcc                                                                                                                <  8:160289/150‑1 (MQ=255)
                                    aCCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCg                                                                                                               >  2:176088/1‑151 (MQ=255)
                                    aCCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCg                                                                                                               <  1:686982/151‑1 (MQ=255)
                                       gTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGctc                                                                                                            >  6:481505/1‑151 (MQ=255)
                                           ttCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCTTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGctct                                                                                                           <  7:1117028/148‑1 (MQ=255)
                                            tCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGc                                                                                                        <  2:1225148/150‑1 (MQ=255)
                                             caccacAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGcc                                                                                                       <  5:1676836/150‑1 (MQ=255)
                                               ccacAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCcgc                                                                                                    <  4:687755/151‑1 (MQ=255)
                                                  caATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGAc                                                                                                 >  4:81971/1‑151 (MQ=255)
                                                  caATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGAc                                                                                                 <  6:1062518/151‑1 (MQ=255)
                                                        tCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGcc                                                                                           >  7:1458094/1‑151 (MQ=255)
                                                         cGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGccc                                                                                          >  4:1150007/1‑151 (MQ=255)
                                                          ggCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCg                                                                                         >  6:985368/1‑151 (MQ=255)
                                                           gCGACATCGCCGACAGCGGGCGGTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGt                                                                                        <  7:995435/151‑1 (MQ=255)
                                                            cGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGt                                                                                        >  8:1645251/1‑150 (MQ=255)
                                                               cATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCg                                                                                    >  4:1321638/1‑151 (MQ=255)
                                                                  cgacgaCAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAg                                                                                 <  3:620104/151‑1 (MQ=255)
                                                                   gacgacAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAgg                                                                                >  7:1594103/1‑151 (MQ=255)
                                                                   gacgacAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAgg                                                                                >  3:92261/1‑151 (MQ=255)
                                                                    acgacAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAggt                                                                               >  4:14782/1‑151 (MQ=255)
                                                                      gacAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAggtgg                                                                             >  5:1215425/1‑151 (MQ=255)
                                                                          gCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGtt                                                                         <  7:1653500/151‑1 (MQ=255)
                                                                           cGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTc                                                                        <  1:76914/151‑1 (MQ=255)
                                                                             ggCGTGTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAg                                                                      <  1:1188327/151‑1 (MQ=255)
                                                                               cGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCg                                                                    >  4:497686/1‑151 (MQ=255)
                                                                                gTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGt                                                                   <  2:1023651/151‑1 (MQ=255)
                                                                                gTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGt                                                                   >  1:1080346/1‑151 (MQ=255)
                                                                                    gTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAg                                                               <  6:210484/151‑1 (MQ=255)
                                                                                      tCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAgg                                                              <  5:1589965/150‑1 (MQ=255)
                                                                                         gCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTcac                                                          >  8:1254317/1‑151 (MQ=255)
                                                                                              aCGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTcaccgcca                                                     <  4:1549481/151‑1 (MQ=255)
                                                                                              aCGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTcaccgcca                                                     <  4:1030683/151‑1 (MQ=255)
                                                                                               cGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTcaccgccac                                                    <  8:1085341/151‑1 (MQ=255)
                                                                                                ggCGTTGGCGTCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTcaccgccacc                                                   >  8:440795/1‑151 (MQ=255)
                                                                                                  ggTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGGTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTcaccgccaccgc                                                 <  1:1279382/150‑1 (MQ=255)
                                                                                                   gTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACcgcg                                                >  1:1469279/1‑151 (MQ=255)
                                                                                                        cATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTAcc                                           >  5:1434673/1‑151 (MQ=255)
                                                                                                           cACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATc                                        >  8:1471267/1‑151 (MQ=255)
                                                                                                            aCCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCt                                       <  1:1671748/151‑1 (MQ=255)
                                                                                                             acGCCCACCATCCCTTAATCTTTCTTGACGCCCGGTTTGTCGGAGAAATCATCCATCTGGTTCTTACGCAGAATACCGCTCGCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACGGCCACCGCGTTACCATCtt                                      <  6:399095/150‑1 (MQ=255)
                                                                                                              cGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCttt                                     >  6:1146042/1‑151 (MQ=255)
                                                                                                               gCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCttt                                     >  4:919550/1‑150 (MQ=255)
                                                                                                                 ccaccaGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATc                                  >  2:143170/1‑151 (MQ=255)
                                                                                                                 ccaccaGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATc                                  >  5:990759/1‑151 (MQ=255)
                                                                                                                  caccaGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATcc                                 <  8:1522803/151‑1 (MQ=255)
                                                                                                                    ccaGCCCGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATccac                               <  8:855553/151‑1 (MQ=255)
                                                                                                                         ccGTAAACGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTg                          >  2:798032/1‑151 (MQ=255)
                                                                                                                             aaaCGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCCGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGt                       >  3:1108490/1‑150 (MQ=255)
                                                                                                                              aaCGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTaa                     <  7:911062/151‑1 (MQ=255)
                                                                                                                                cGTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATCCCCGTACCGAAGCTGGTGTTCAGCGTATAGGTCAACGCCACCGCGTTACCATCTTTATCAACCACTGAGTAATg                   >  1:1344109/1‑151 (MQ=255)
                                                                                                                                 gTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATgg                  <  4:1368554/151‑1 (MQ=255)
                                                                                                                                 gTTCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATgg                  <  3:761960/151‑1 (MQ=255)
                                                                                                                                  ttCGGTACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATggg                 >  7:1344765/1‑151 (MQ=255)
                                                                                                                                       tACGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATGGGTAGtt            >  2:1074254/1‑151 (MQ=255)
                                                                                                                                        aCGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATGGGTAGttt           <  4:760809/151‑1 (MQ=255)
                                                                                                                                        aCGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATGGGTAGttt           <  4:630502/151‑1 (MQ=255)
                                                                                                                                        aCGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATGGGTAGtt            <  3:819353/150‑1 (MQ=255)
                                                                                                                                         cGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATGGGTAGtttt          >  2:478468/1‑150 (MQ=255)
                                                                                                                                         cGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTg          >  6:165891/1‑151 (MQ=255)
                                                                                                                                         cGCCCGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTg          <  4:275377/151‑1 (MQ=255)
                                                                                                                                           cccGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGAt        >  8:641187/1‑151 (MQ=255)
                                                                                                                                             cGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGAtt       >  3:453192/1‑150 (MQ=255)
                                                                                                                                             cGGTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTa      <  7:623789/151‑1 (MQ=255)
                                                                                                                                              ggTTTGACGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTGACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTAc     >  2:897669/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCTACCGCCTGGGCTACCGGTAACCAGCCAGGTTTTACCGTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCT  >  NC_000913/3585369‑3585664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑