Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,587,531 G→A L195F (CTC→TTC)  ugpQ ← glycerophosphodiester phosphodiesterase, cytosolic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,587,5310GA96.5% 1443.3 / 23.3 431L195F (CTC→TTC) ugpQglycerophosphodiester phosphodiesterase, cytosolic
Reads supporting (aligned to +/- strand):  ref base G (6/9);  new base A (215/201);  total (221/210)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 431 total aligned reads displayed.

AGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGAC  >  NC_000913/3587384‑3587679
                                                                                                                                                   |                                                                                                                                                    
  ttCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCCGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCGTTCAACTGCATCACTCGCGCTTTATTGAATAACt                                                                                                                                                 >  6:338700/1‑151 (MQ=255)
   tCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACt                                                                                                                                                 <  8:225425/150‑1 (MQ=255)
   tCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACt                                                                                                                                                 <  1:660568/150‑1 (MQ=255)
    cGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTa                                                                                                                                               <  5:883361/151‑1 (MQ=255)
     ggACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTAt                                                                                                                                              >  4:249026/1‑151 (MQ=255)
      gACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATg                                                                                                                                             <  6:645247/151‑1 (MQ=255)
      gACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATg                                                                                                                                             <  4:574903/151‑1 (MQ=255)
      gACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATg                                                                                                                                             <  2:1137656/151‑1 (MQ=255)
       aCCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGa                                                                                                                                            <  2:917069/151‑1 (MQ=255)
        ccAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGAt                                                                                                                                           <  4:1232001/151‑1 (MQ=255)
         cAATCACGTCAATCGGATCGGTACAGTTGCAATCCACACCCCAGCGCAGGAACTCTGCTGCGCGCTGGGGTTTATTGACTTTATAAACCAGAATCCCCAGTCCGGCGTGTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGAtt                                                                                                                                          <  2:699951/151‑1 (MQ=255)
         cAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGAtt                                                                                                                                          <  3:1599291/151‑1 (MQ=255)
          aaTCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTg                                                                                                                                         >  8:844817/1‑151 (MQ=255)
           aTCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTga                                                                                                                                        <  5:1283351/151‑1 (MQ=255)
             cACGTCAGTCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTgag                                                                                                                                       <  3:1164818/150‑1 (MQ=255)
             aaCGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTgaga                                                                                                                                      <  4:1475859/150‑1 (MQ=255)
                gacAATCGCATCGCTACAGCTGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCCCACCGCTTAATTCCCGCAATAAACCAGAATCCGCACTCCCGCCACAGTCAACACCATCACACGCGCTGTAATGAGTAACTTAAGAATGAGATg                                                                                                                                    >  6:246220/3‑150 (MQ=255)
                gTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGa                                                                                                                                   <  2:134420/151‑1 (MQ=255)
                  cAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCATCTCTGCTGCGCGCTTGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGaa                                                                                                                                  <  4:1096425/150‑1 (MQ=255)
                  cAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGaa                                                                                                                                  <  6:1304823/150‑1 (MQ=255)
                  cAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAAt                                                                                                                                 <  4:898165/151‑1 (MQ=255)
                   aaTCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACACTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCCCTTAATTGAATAAGTTATGATTGAGATGAAt                                                                                                                                 >  1:367049/1‑150 (MQ=255)
                    aTCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATag                                                                                                                               <  7:1348454/151‑1 (MQ=255)
                      cGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATag                                                                                                                               >  3:651509/1‑149 (MQ=255)
                       gCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAgaga                                                                                                                            <  3:575810/151‑1 (MQ=255)
                       gCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAgaga                                                                                                                            <  6:1380232/151‑1 (MQ=255)
                       ccATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAgaga                                                                                                                            <  1:872394/150‑1 (MQ=255)
                         aTCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACg                                                                                                                          <  2:125167/151‑1 (MQ=255)
                          tCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGc                                                                                                                         >  2:1067111/1‑151 (MQ=255)
                            ggTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGc                                                                                                                         >  8:1469608/1‑149 (MQ=255)
                             gTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGcagc                                                                                                                      >  1:5339/1‑151 (MQ=255)
                             gTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGcagc                                                                                                                      >  6:119393/1‑151 (MQ=255)
                              tACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTTTAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGAATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGcagcc                                                                                                                     >  1:72881/1‑151 (MQ=255)
                              tACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATATAGACGcagcc                                                                                                                     >  7:895021/1‑151 (MQ=255)
                                 aGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGcagccc                                                                                                                    >  6:984167/1‑149 (MQ=255)
                                   aTGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGcagcccagcc                                                                                                                <  2:315853/151‑1 (MQ=255)
                                     gCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCcgc                                                                                                              >  3:974552/1‑151 (MQ=255)
                                     gCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAAATGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCcgc                                                                                                              <  7:390529/151‑1 (MQ=255)
                                      cAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCcgcg                                                                                                             >  7:1602489/1‑151 (MQ=255)
                                      cAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCcgcg                                                                                                             <  3:1293552/151‑1 (MQ=255)
                                          ccACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCgg                                                                                                          <  1:1457790/150‑1 (MQ=255)
                                           cacaCCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTc                                                                                                        <  3:415676/151‑1 (MQ=255)
                                              acCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGt                                                                                                     >  3:172433/1‑151 (MQ=255)
                                               ccccAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGtt                                                                                                    <  1:953796/151‑1 (MQ=255)
                                                cccAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTc                                                                                                   >  1:1209990/1‑151 (MQ=255)
                                                 ccAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTcg                                                                                                  >  6:841945/1‑151 (MQ=255)
                                                   aGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTcgcg                                                                                                >  8:309561/1‑151 (MQ=255)
                                                     cgcAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGcc                                                                                              <  2:1497542/151‑1 (MQ=255)
                                                      gcagcaACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTcgaccc                                                                                              >  1:227778/1‑146 (MQ=255)
                                                            aCTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATTATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAgtcgtc                                                                                       <  5:1417315/151‑1 (MQ=255)
                                                            aCTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAgtcgtc                                                                                       <  3:1042272/151‑1 (MQ=255)
                                                            aCTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAgtcgtc                                                                                       <  5:576564/151‑1 (MQ=255)
                                                              gctgctgCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTcgc                                                                                     <  1:1025279/150‑1 (MQ=255)
                                                                tgctgcGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCgcgc                                                                                   >  7:729156/1‑151 (MQ=255)
                                                                   tgcGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCAc                                                                                >  7:500127/1‑151 (MQ=255)
                                                                    gcgctcTGGGGTTTATTGACGGTATATACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGTGAGGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACt                                                                               <  1:1546330/151‑1 (MQ=255)
                                                                       cgcTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCAt                                                                            >  8:1404477/1‑151 (MQ=255)
                                                                                ttATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGcaac                                                                    >  4:341093/1‑150 (MQ=255)
                                                                                  aTTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGcaacaa                                                                  >  3:760803/1‑150 (MQ=255)
                                                                                     gACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACcg                                                              >  7:957375/1‑151 (MQ=255)
                                                                                      aCGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACcgc                                                             <  4:1168532/151‑1 (MQ=255)
                                                                                         gTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACcgcgcg                                                          >  3:317564/1‑151 (MQ=255)
                                                                                             aaaCCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGc                                                        >  8:1520901/1‑149 (MQ=255)
                                                                                             aaaCCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCa                                                       <  5:678941/150‑1 (MQ=255)
                                                                                              acccagtctcccgaGTCCGCCGGCTTTCAACTGCATCCCTCGCGCTTTATTGAATAACTTATGATTGAGATGACGAGATCCGCCGCGCAGCCGCGCGGGCAGTTCGCGGCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGt                                                     <  5:103027/138‑1 (MQ=255)
                                                                                              aaCCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATTATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGt                                                     >  8:1040890/1‑151 (MQ=255)
                                                                                                 cAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTcc                                                  <  4:446745/151‑1 (MQ=255)
                                                                                                  aGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCg                                                 >  4:788710/1‑151 (MQ=255)
                                                                                                   gAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCgg                                                <  6:174406/151‑1 (MQ=255)
                                                                                                     aTCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCCCTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTg                                              >  4:1582167/1‑151 (MQ=255)
                                                                                                     aTCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTg                                              <  7:628708/151‑1 (MQ=255)
                                                                                                     aTCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTg                                              <  8:664251/151‑1 (MQ=255)
                                                                                                      tCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTgc                                             >  5:39642/1‑151 (MQ=255)
                                                                                                        cGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTgccg                                           <  6:1465439/151‑1 (MQ=255)
                                                                                                        cGCAGTCCGGCGTCTGTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTgccg                                           >  5:265245/1‑151 (MQ=255)
                                                                                                         gCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTgccgc                                          <  6:747683/151‑1 (MQ=255)
                                                                                                          cAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTgccgcc                                         >  6:1051548/1‑151 (MQ=255)
                                                                                                           aGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCt                                        <  6:956329/151‑1 (MQ=255)
                                                                                                               cGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCtgttg                                    <  7:1412075/151‑1 (MQ=255)
                                                                                                                ggCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCtgttgt                                   >  8:421340/1‑151 (MQ=255)
                                                                                                                     cTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGc                              >  3:883087/1‑151 (MQ=255)
                                                                                                                       ttCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGATCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCtt                            >  8:736031/1‑151 (MQ=255)
                                                                                                                         cAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTc                           <  4:396641/150‑1 (MQ=255)
                                                                                                                         cAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCt                          <  5:1569479/151‑1 (MQ=255)
                                                                                                                            cTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTaa                        >  5:488021/1‑150 (MQ=255)
                                                                                                                              gCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGc                     <  3:514676/151‑1 (MQ=255)
                                                                                                                                aTCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCAt                   >  8:1225525/1‑151 (MQ=255)
                                                                                                                                aTCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCAt                   >  2:519504/1‑151 (MQ=255)
                                                                                                                                   aCTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCa                 >  7:178828/1‑150 (MQ=255)
                                                                                                                                      cgcgCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAAtct             >  4:205182/1‑151 (MQ=255)
                                                                                                                                      cgcgCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAAt               <  6:1558525/149‑1 (MQ=255)
                                                                                                                                        cgcTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAAtctc            >  1:136796/1‑150 (MQ=255)
                                                                                                                                        cgcTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAAtctc            >  8:1342996/1‑150 (MQ=255)
                                                                                                                                         gcTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCaa          <  7:1543639/151‑1 (MQ=255)
                                                                                                                                         gcTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCa           >  2:1571243/1‑150 (MQ=255)
                                                                                                                                          cTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCaa          >  8:477690/1‑150 (MQ=255)
                                                                                                                                          cTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCaa          >  7:1146527/1‑150 (MQ=255)
                                                                                                                                            ttATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCaaa         <  8:1604884/149‑1 (MQ=255)
                                                                                                                                             tATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACg       >  2:1686977/1‑150 (MQ=255)
                                                                                                                                                tGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATg  >  7:6732/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGAC  >  NC_000913/3587384‑3587679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: