Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,588,787 G→A P132S (CCG→TCG)  ugpC ← sn‑glycerol‑3‑phosphate ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,588,7870GA96.3% 1337.9 / 24.2 412P132S (CCG→TCG) ugpCsn‑glycerol‑3‑phosphate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base G (8/7);  new base A (156/237);  total (167/245)
Fisher's exact test for biased strand distribution p-value = 2.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 416 total aligned reads displayed.

TGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTG  >  NC_000913/3588639‑3588934
                                                                                                                                                    |                                                                                                                                                   
tGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGc                                                                                                                                                   >  1:1011738/1‑151 (MQ=255)
tGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGc                                                                                                                                                   >  8:1549416/1‑151 (MQ=255)
ggCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCACACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGc                                                                                                                                                   <  4:1252381/150‑1 (MQ=255)
     gtGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGt                                                                                                                                              <  5:134142/150‑1 (MQ=255)
       gTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGtt                                                                                                                                             <  8:463521/150‑1 (MQ=255)
         tccAGTTCACGAGGCAGCTCCACGCGCTGGTGGGCATCGCGGTTCGCGACCGGCGCATCAAACAGCACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGa                                                                                                                                          <  1:1398396/148‑1 (MQ=255)
         tGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTg                                                                                                                                           >  8:713464/1‑150 (MQ=255)
         tGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTg                                                                                                                                           >  2:548940/1‑150 (MQ=255)
          gCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTg                                                                                                                                           >  2:1386005/1‑149 (MQ=255)
          gCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAg                                                                                                                                         <  7:469682/151‑1 (MQ=255)
            aGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGc                                                                                                                                        >  6:1236756/1‑150 (MQ=255)
              ttCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTGTGAGc                                                                                                                                        >  2:1338538/1‑148 (MQ=255)
                 aaGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCg                                                                                                                                  >  6:527580/1‑151 (MQ=255)
                 aaGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCg                                                                                                                                  <  5:672677/151‑1 (MQ=255)
                   gACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGCCCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTc                                                                                                                                >  1:1562223/1‑151 (MQ=255)
                    aCGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTcc                                                                                                                               <  2:596687/151‑1 (MQ=255)
                     cGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCa                                                                                                                              <  2:1091938/151‑1 (MQ=255)
                     cGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCa                                                                                                                              <  1:1479970/151‑1 (MQ=255)
                          cTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTcc                                                                                                                         >  8:326893/1‑151 (MQ=255)
                          cTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCACGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCCCCGGAAAGCTCCCGCGAGCGCCGTATGAGCAGACCGTCCAGCTcc                                                                                                                         >  3:1443221/1‑151 (MQ=255)
                            gCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAg                                                                                                                       <  4:197489/151‑1 (MQ=255)
                            gCAAGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTTGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTc                                                                                                                          <  7:140438/148‑1 (MQ=255)
                              aCGGGCAGGTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACTGGAACACCGCCGGATCGCGCAGAATCGCGCTGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGaa                                                                                                                     <  3:1562887/151‑1 (MQ=255)
                               cgcgCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAAt                                                                                                                    <  6:392473/151‑1 (MQ=255)
                               cgcgCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAAt                                                                                                                    <  8:1172777/151‑1 (MQ=255)
                                gcgcAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATg                                                                                                                   <  4:643028/151‑1 (MQ=255)
                                 cgcAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATgc                                                                                                                  <  2:905155/151‑1 (MQ=255)
                                  gcAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATgcg                                                                                                                 >  3:777708/1‑151 (MQ=255)
                                     gCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGAGCGGTCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATgcgcgc                                                                                                              >  8:625687/1‑151 (MQ=255)
                                        tGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGcgccgc                                                                                                           >  1:681205/1‑151 (MQ=255)
                                        tGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGcgccg                                                                                                            <  5:1492446/150‑1 (MQ=255)
                                          gCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGctt                                                                                                         >  2:1188231/1‑151 (MQ=255)
                                           cagcgcggTTAGAGAGCGGCTCATCAAACAGCACCACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGcttc                                                                                                        <  1:946798/145‑1 (MQ=255)
                                            aTCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGcttct                                                                                                       >  8:913307/1‑151 (MQ=255)
                                              cGAGGTTAGAGAGCGGCTCATCAAACAGGACCACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCttt                                                                                                     <  5:687215/151‑1 (MQ=255)
                                                aGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTaa                                                                                                   <  8:1048592/151‑1 (MQ=255)
                                                aGGTTAGAGAGCGGCTCATCAAAAAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCCCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGAATCTTTa                                                                                                    >  8:782170/1‑150 (MQ=255)
                                                 ggTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAAc                                                                                                  <  5:1028474/151‑1 (MQ=255)
                                                  gTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAAcg                                                                                                 <  3:680325/151‑1 (MQ=255)
                                                       cgagCGGCGCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTc                                                                                            <  4:1379978/150‑1 (MQ=255)
                                                          gCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGc                                                                                         <  3:200811/151‑1 (MQ=255)
                                                            ggCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCTCACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCaa                                                                                       <  1:771658/151‑1 (MQ=255)
                                                              cTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAAtt                                                                                     >  7:1090449/1‑151 (MQ=255)
                                                               tcatcaAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAAttt                                                                                    >  3:1210354/1‑151 (MQ=255)
                                                               gcatcaAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCACACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAAttt                                                                                    <  8:1345483/150‑1 (MQ=255)
                                                                catcaAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAAtt                                                                                     <  1:570608/149‑1 (MQ=255)
                                                                catcaAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAAtt                                                                                     <  6:1624195/149‑1 (MQ=255)
                                                                 atcaAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTtgc                                                                                  <  6:636592/151‑1 (MQ=255)
                                                                  tcaAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTtgct                                                                                 >  3:1120555/1‑151 (MQ=255)
                                                                    aaaCAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTtgctgc                                                                               <  3:513792/151‑1 (MQ=255)
                                                                     aaCAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTgctgct                                                                              <  8:871884/151‑1 (MQ=255)
                                                                      aCAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGAGGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCtt                                                                             <  4:294637/151‑1 (MQ=255)
                                                                      aCAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCtt                                                                             >  3:610840/1‑151 (MQ=255)
                                                                      aCAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCtt                                                                             <  1:608171/151‑1 (MQ=255)
                                                                      aCAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCtt                                                                             <  5:614476/151‑1 (MQ=255)
                                                                       cAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTg                                                                            <  5:784146/151‑1 (MQ=255)
                                                                         ggAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGcc                                                                          >  8:244428/1‑151 (MQ=255)
                                                                           aaCACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGccc                                                                         >  8:222064/1‑150 (MQ=255)
                                                                           aaCACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCa                                                                        <  6:1410411/151‑1 (MQ=255)
                                                                             cacCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATg                                                                      <  6:270655/151‑1 (MQ=255)
                                                                                  cctgATCTCTCACAATCGCGCGGCCCATCTCCACACGCTGGCGCTGACCGCAGTAAAGCTCGCTCGAGCGACGTTTGAGCAGACCGTCCAGATCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCcgcg                                                                 <  7:985037/148‑1 (MQ=255)
                                                                                  ccgGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCcgcg                                                                 <  4:74127/151‑1 (MQ=255)
                                                                                            cacaATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGc                                                       >  5:236961/1‑151 (MQ=255)
                                                                                              caATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGccc                                                     >  8:1455941/1‑151 (MQ=255)
                                                                                              caATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGccc                                                     <  4:444402/151‑1 (MQ=255)
                                                                                              caATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGccc                                                     >  4:883869/1‑151 (MQ=255)
                                                                                               aaTCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGcccc                                                    <  5:1132082/151‑1 (MQ=255)
                                                                                                aTCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGccccc                                                   <  6:1584663/151‑1 (MQ=255)
                                                                                                  cgcgcgGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCa                                                  <  7:204106/150‑1 (MQ=255)
                                                                                                   gcgcgGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACg                                                <  8:1616434/151‑1 (MQ=255)
                                                                                                     gcgGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGcc                                              >  3:1398198/1‑151 (MQ=255)
                                                                                                        gCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATg                                           <  7:565716/151‑1 (MQ=255)
                                                                                                        gCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATg                                           <  2:1628182/151‑1 (MQ=255)
                                                                                                        gCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATg                                           <  3:1650529/151‑1 (MQ=255)
                                                                                                          ccATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGtt                                         <  4:366939/151‑1 (MQ=255)
                                                                                                          ccATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGtt                                         <  7:278492/151‑1 (MQ=255)
                                                                                                          ccATCGCCACACGCTGGCGATGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGTCCAGCTCCAGACTGCGCGCCCCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCACACGCCATGtt                                         >  2:78246/1‑151 (MQ=255)
                                                                                                           cATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGttt                                        >  8:1270567/1‑151 (MQ=255)
                                                                                                           cATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGttt                                        <  2:197866/151‑1 (MQ=255)
                                                                                                            aTCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGtttt                                       >  3:1529502/1‑151 (MQ=255)
                                                                                                                ccACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTttcttc                                   >  3:974000/1‑151 (MQ=255)
                                                                                                                         ggCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCat                          <  7:718382/151‑1 (MQ=255)
                                                                                                                          gcgcTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCata                         <  2:1247820/151‑1 (MQ=255)
                                                                                                                           cgcTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACTCGCTCTTCAATTTGCTGATTGCCCATGCCGCGAATTTTCAGCCCCCACTCCATTTTTTCTTCGACACTCatat                        <  1:1255820/151‑1 (MQ=255)
                                                                                                                                aCCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATATTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCgg                    >  6:758681/1‑150 (MQ=255)
                                                                                                                                  cgccgGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATa                 <  1:252970/151‑1 (MQ=255)
                                                                                                                                   gccgGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATa                 >  1:841780/1‑150 (MQ=255)
                                                                                                                                    ccgGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATaaa               <  6:1334990/151‑1 (MQ=255)
                                                                                                                                     cgGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAg              <  5:552672/151‑1 (MQ=255)
                                                                                                                                     cgGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGACGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATaa                >  1:1138942/1‑149 (MQ=255)
                                                                                                                                      ggAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCCCGCAATGTTTTCTTCGACACTCATATGCGGATaacgg             >  2:1537465/1‑148 (MQ=255)
                                                                                                                                      ggAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAgc             <  2:974387/151‑1 (MQ=255)
                                                                                                                                      ggAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAgc             <  5:1393876/151‑1 (MQ=255)
                                                                                                                                      ggAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAgc             >  7:1625422/1‑151 (MQ=255)
                                                                                                                                      ggAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAgc             <  3:334938/151‑1 (MQ=255)
                                                                                                                                         aaGCTCGCGCGGGCGACGTTTGAGCAGACCGTCCAGCTCGAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGcgcg          <  3:571636/151‑1 (MQ=255)
                                                                                                                                              cgcgcgAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAg       <  7:775543/149‑1 (MQ=255)
                                                                                                                                               gcgcgGGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTc    >  2:3267/1‑151 (MQ=255)
                                                                                                                                                 gcgagcgaCGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTg  >  2:439339/1‑151 (MQ=255)
                                                                                                                                                 gcgagcgaCGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTg  <  5:947564/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTG  >  NC_000913/3588639‑3588934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: