Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,589,956 G→A L24L (CTC→CTT ugpE ← sn‑glycerol‑3‑phosphate ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,589,9560GA97.3% 1502.1 / 18.6 442L24L (CTC→CTTugpEsn‑glycerol‑3‑phosphate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (8/4);  new base A (200/230);  total (208/234)
Fisher's exact test for biased strand distribution p-value = 2.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01

Only 100 of 444 total aligned reads displayed.

AGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGAC  >  NC_000913/3589808‑3590105
                                                                                                                                                    |                                                                                                                                                     
  aaCGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAgg                                                                                                                                                    >  3:226833/1‑150 (MQ=255)
  aaCGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAgg                                                                                                                                                    >  2:216402/1‑150 (MQ=255)
  aaCGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGa                                                                                                                                                   <  4:512389/151‑1 (MQ=255)
    cGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATc                                                                                                                                                 >  8:80189/1‑151 (MQ=255)
      gcgcgcTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCAc                                                                                                                                               <  8:1131738/151‑1 (MQ=255)
      gcgcgcTATTCGTGCCTACCCCGT‑‑ACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACcg                                                                                                                                             <  7:1594130/151‑1 (MQ=255)
       cgcgcTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCAcc                                                                                                                                              >  7:53989/1‑151 (MQ=255)
        gcgcTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACcg                                                                                                                                             <  6:406030/151‑1 (MQ=255)
          gcTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACcgcg                                                                                                                                           <  8:1230109/151‑1 (MQ=255)
           cTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACcgcg                                                                                                                                           >  4:313201/1‑150 (MQ=255)
            tATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGCATGAGCGTCAACGGCGCGGCATAGACGGCCTGGATATCAAGCGTCGCCGCCACAAACGCCACGTACAGCGGGAAAACGATCACCACGAt                                                                                                                                         >  6:1458145/1‑151 (MQ=255)
             aTTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCAAAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATc                                                                                                                                        <  1:822690/151‑1 (MQ=255)
              ttCGTGCCTACGCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGAGGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATc                                                                                                                                        <  6:1637270/150‑1 (MQ=255)
                 gTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGa                                                                                                                                    <  3:385930/151‑1 (MQ=255)
                  tGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGACCACCGCGATCCCg                                                                                                                                     >  4:1660960/1‑149 (MQ=255)
                  tGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCAGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCg                                                                                                                                     >  2:614866/1‑149 (MQ=255)
                  ggCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAg                                                                                                                                   <  5:458988/150‑1 (MQ=255)
                   gCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAgg                                                                                                                                  <  7:663703/151‑1 (MQ=255)
                   gCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAg                                                                                                                                   <  8:1471383/150‑1 (MQ=255)
                    ccTACCCCGTTCACCCAGATGTTGTGGATGTTTTCAAGCAGATGTGTGCCGGGGATGAGCGTCATGGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAgg                                                                                                                                  <  1:1452089/150‑1 (MQ=255)
                     cTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGAt                                                                                                                                >  6:591495/1‑151 (MQ=255)
                         cccGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGc                                                                                                                            <  4:648852/151‑1 (MQ=255)
                         cccGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGc                                                                                                                            <  6:916068/151‑1 (MQ=255)
                          ccGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGc                                                                                                                            >  3:1525081/1‑150 (MQ=255)
                          ccGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCa                                                                                                                           <  7:247298/151‑1 (MQ=255)
                              tCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGt                                                                                                                       >  4:1265404/1‑151 (MQ=255)
                              tCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGt                                                                                                                       >  2:1484096/1‑151 (MQ=255)
                              tCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCACGAGGATCAGCATgg                                                                                                                        <  7:396159/150‑1 (MQ=255)
                                aCCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTAt                                                                                                                     <  6:1049682/151‑1 (MQ=255)
                                aCCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGACCACCGCGATCCCGAGGATCAGCATGGTAt                                                                                                                     <  7:1653478/151‑1 (MQ=255)
                                  ccAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATgg                                                                                                                   >  4:759329/1‑151 (MQ=255)
                                        gttgtGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGaa                                                                                                              <  6:180200/150‑1 (MQ=255)
                                        gttgtGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAat                                                                                                             <  6:1593539/151‑1 (MQ=255)
                                             ggATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGt                                                                                                        >  5:1424446/1‑151 (MQ=255)
                                              gATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTc                                                                                                       <  3:904906/151‑1 (MQ=255)
                                              gATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTc                                                                                                       <  4:241600/151‑1 (MQ=255)
                                               aTGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCa                                                                                                      <  1:1675210/151‑1 (MQ=255)
                                                tGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTc                                                                                                       >  7:93145/1‑149 (MQ=255)
                                                   tttCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGcc                                                                                                   <  4:1548489/150‑1 (MQ=255)
                                                   tttCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGcc                                                                                                   >  8:1533401/1‑150 (MQ=255)
                                                    ttCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGGCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCa                                                                                                  <  8:296214/150‑1 (MQ=255)
                                                     tCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCacg                                                                                                >  3:1013768/1‑151 (MQ=255)
                                                     tCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCac                                                                                                 <  2:398286/150‑1 (MQ=255)
                                                       cagcagATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCacgga                                                                                              <  1:265273/151‑1 (MQ=255)
                                                        agcagATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCacggac                                                                                             <  4:142795/151‑1 (MQ=255)
                                                        agcagATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCacggac                                                                                             <  7:1308511/151‑1 (MQ=255)
                                                               gtgtgCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTtctc                                                                                      <  4:1022419/151‑1 (MQ=255)
                                                                tgtgCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGCCTGAATATCGTCAGCCACGGACGGTtctc                                                                                      >  6:225883/1‑150 (MQ=255)
                                                                 gtgCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCa                                                                                     >  3:1387540/1‑150 (MQ=255)
                                                                       gggATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTg                                                                              <  4:159002/151‑1 (MQ=255)
                                                                        ggATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTgg                                                                             <  5:705642/151‑1 (MQ=255)
                                                                           tGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTa                                                                           >  1:1014099/1‑150 (MQ=255)
                                                                            gAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTaa                                                                          <  6:1334777/150‑1 (MQ=255)
                                                                             aGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACg                                                                        >  7:1509822/1‑151 (MQ=255)
                                                                              gCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGc                                                                       <  3:563785/151‑1 (MQ=255)
                                                                              ccGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGc                                                                       <  6:1130952/150‑1 (MQ=255)
                                                                                   aTCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCtt                                                                  <  1:337261/151‑1 (MQ=255)
                                                                                   aTCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCtt                                                                  >  5:1649228/1‑151 (MQ=255)
                                                                                     cGGCGCGGCATAGACGGCCTGTTTATCCAGGGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGAAGGTTCTCAATCATTGGTAATGCACCTTg                                                                 <  1:544479/150‑1 (MQ=255)
                                                                                     cGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTg                                                                 <  6:447603/150‑1 (MQ=255)
                                                                                     cGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGc                                                                <  5:59294/151‑1 (MQ=255)
                                                                                       gcgcGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCtt                                                              >  5:339764/1‑151 (MQ=255)
                                                                                       gcgcGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCAAGTACAGCGGGAAAGGGATCACCGCTATCCCGAGGATCAGCATTGTATTGCTGAATATCTTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCtt                                                              <  3:71041/151‑1 (MQ=255)
                                                                                        cgcgGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCttt                                                             >  7:1219029/1‑151 (MQ=255)
                                                                                        cgcgGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCttt                                                             <  2:1302774/151‑1 (MQ=255)
                                                                                         gcgGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTc                                                            >  5:875190/1‑151 (MQ=255)
                                                                                          cgGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCa                                                           <  5:1234691/151‑1 (MQ=255)
                                                                                            gCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAAc                                                         >  1:616922/1‑151 (MQ=255)
                                                                                            gCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAAc                                                         >  6:361487/1‑151 (MQ=255)
                                                                                                aGACGGCCTGTTTACCCAGCCTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGAGCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATa                                                      <  2:1192149/150‑1 (MQ=255)
                                                                                                 gACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGc                                                    >  5:1130966/1‑151 (MQ=255)
                                                                                                   cGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTAGAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGAAGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCg                                                   <  7:839190/150‑1 (MQ=255)
                                                                                                   cGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCgg                                                  <  7:1051955/151‑1 (MQ=255)
                                                                                                    ggCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGa                                                 <  2:158182/151‑1 (MQ=255)
                                                                                                     gCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGa                                                 >  1:905565/1‑150 (MQ=255)
                                                                                                               ccAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCaccacc                                      >  4:1316901/1‑151 (MQ=255)
                                                                                                                cAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGTAACGGGACCACCg                                     <  1:1212102/151‑1 (MQ=255)
                                                                                                                   cGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCa                                  <  8:1325961/151‑1 (MQ=255)
                                                                                                                       gccgcGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCCCCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCAc                              >  6:503301/1‑151 (MQ=255)
                                                                                                                       gccgcGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCAc                              <  1:1115374/151‑1 (MQ=255)
                                                                                                                          gcgACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGAt                           <  8:1059690/151‑1 (MQ=255)
                                                                                                                            gACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATg                          >  4:1162135/1‑150 (MQ=255)
                                                                                                                            gACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGa                         <  3:179520/151‑1 (MQ=255)
                                                                                                                              cAAACTCCACGTACAGCGTGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACg                       <  1:1371535/151‑1 (MQ=255)
                                                                                                                               aaaCGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACg                       >  8:668177/1‑150 (MQ=255)
                                                                                                                               aaaCGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACg                       >  2:508417/1‑150 (MQ=255)
                                                                                                                                aaCGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACg                       >  7:784729/1‑149 (MQ=255)
                                                                                                                                 aCGCCACGTAGATCGGGAAAAGGAGCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAgg                    <  8:602244/151‑1 (MQ=255)
                                                                                                                                  cGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAgg                    >  8:73405/1‑150 (MQ=255)
                                                                                                                                   gCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGaa                  <  2:834425/151‑1 (MQ=255)
                                                                                                                                   gCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGa                   >  4:868704/1‑150 (MQ=255)
                                                                                                                                    ccACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAAc                 <  8:459131/151‑1 (MQ=255)
                                                                                                                                     cACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAAc                 >  7:1381805/1‑150 (MQ=255)
                                                                                                                                      aCGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACAt               >  1:376334/1‑151 (MQ=255)
                                                                                                                                       cGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATc              >  5:221652/1‑151 (MQ=255)
                                                                                                                                        gTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCa             <  3:110245/151‑1 (MQ=255)
                                                                                                                                          aCAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCaa            <  3:1446237/150‑1 (MQ=255)
                                                                                                                                          aCAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCaa            <  6:727307/150‑1 (MQ=255)
                                                                                                                                               gggAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAAcaccac      <  7:374439/151‑1 (MQ=255)
                                                                                                                                                   agagGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGAc  <  1:398882/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGAC  >  NC_000913/3589808‑3590105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: